YBR038W / CHS2

Chitin synthase II; catalyzes transfer of N-acetylglucosamine (GlcNAc) to chitin upon activation of zymogenic form; required for chitin synthesis in the primary septum during cytokinesis; localization regulated by Cdk1p during mitosis; phosphorylation by Dbf2p kinase regulates its dynamics and chitin synthesis during cytokinesis

Zygosity: Heterozygous strain
fixedexpanded
Profile for YBR038W / CHS2

Click on Significant Values for Screen Details ID:SGTC_34|Compound:0469-0683|FD-Score:3.90|P-value:2.17E-4 ID:SGTC_41|Compound:0478-0632|FD-Score:-5.85|P-value:7.79E-8 ID:SGTC_46|Compound:0329-0187a|FD-Score:-5.38|P-value:6.97E-7 ID:SGTC_82|Compound:0054-0107|FD-Score:3.90|P-value:2.13E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:3.97|P-value:1.68E-4 ID:SGTC_531|Compound:3788-1495|FD-Score:4.40|P-value:3.64E-5 ID:SGTC_535|Compound:2293-4129|FD-Score:-4.35|P-value:4.89E-5 ID:SGTC_539|Compound:4859-0462|FD-Score:5.04|P-value:2.72E-6 ID:SGTC_586|Compound:0957-0265|FD-Score:3.75|P-value:3.60E-4 ID:SGTC_753|Compound:1322-0200|FD-Score:-3.62|P-value:5.99E-4 ID:SGTC_818|Compound:4072-2707|FD-Score:4.68|P-value:1.22E-5 ID:SGTC_823|Compound:0417-1665|FD-Score:3.58|P-value:6.19E-4 ID:SGTC_839|Compound:0122-0028|FD-Score:3.57|P-value:6.24E-4 ID:SGTC_923|Compound:1900-2434|FD-Score:3.58|P-value:6.21E-4 ID:SGTC_955|Compound:1216-0110|FD-Score:4.14|P-value:9.21E-5 ID:SGTC_1159|Compound:4092-0538|FD-Score:3.46|P-value:8.82E-4 ID:SGTC_1331|Compound:1472-0049|FD-Score:3.70|P-value:4.17E-4 ID:SGTC_1335|Compound:1486-0900|FD-Score:-3.49|P-value:8.87E-4 ID:SGTC_1469|Compound:k284-2957|FD-Score:-4.41|P-value:3.89E-5 ID:SGTC_1789|Compound:tunicamycin|FD-Score:3.75|P-value:3.51E-4 ID:SGTC_1928|Compound:4'-methoxychalcone|FD-Score:-3.69|P-value:4.83E-4 ID:SGTC_1937|Compound:st074714|FD-Score:3.68|P-value:4.49E-4 ID:SGTC_1940|Compound:st075990|FD-Score:-3.56|P-value:7.23E-4 ID:SGTC_2010|Compound:4012422|FD-Score:-3.69|P-value:4.79E-4 ID:SGTC_2116|Compound:5511452|FD-Score:3.55|P-value:6.71E-4 ID:SGTC_2255|Compound:7941087|FD-Score:-3.88|P-value:2.53E-4 ID:SGTC_2302|Compound:7771753|FD-Score:3.64|P-value:5.01E-4 ID:SGTC_2383|Compound:9036125|FD-Score:4.12|P-value:9.88E-5 ID:SGTC_2419|Compound:paclitaxel|FD-Score:3.46|P-value:8.87E-4 ID:SGTC_2487|Compound:5918373|FD-Score:4.42|P-value:3.27E-5 ID:SGTC_2606|Compound:farnesol|FD-Score:3.81|P-value:2.90E-4 ID:SGTC_2781|Compound:5567276|FD-Score:3.49|P-value:8.03E-4 ID:SGTC_2901|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:3.43|P-value:9.72E-4 ID:SGTC_3080|Compound:9117754|FD-Score:3.51|P-value:7.62E-4 ID:SGTC_3097|Compound:9117246|FD-Score:4.73|P-value:9.93E-6 ID:SGTC_3104|Compound:9120930|FD-Score:3.65|P-value:4.95E-4 ID:SGTC_3162|Compound:9099653|FD-Score:-3.85|P-value:2.87E-4 ID:SGTC_34|Compound:0469-0683|FD-Score:3.90|P-value:2.17E-4 ID:SGTC_41|Compound:0478-0632|FD-Score:-5.85|P-value:7.79E-8 ID:SGTC_46|Compound:0329-0187a|FD-Score:-5.38|P-value:6.97E-7 ID:SGTC_82|Compound:0054-0107|FD-Score:3.90|P-value:2.13E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:3.97|P-value:1.68E-4 ID:SGTC_531|Compound:3788-1495|FD-Score:4.40|P-value:3.64E-5 ID:SGTC_535|Compound:2293-4129|FD-Score:-4.35|P-value:4.89E-5 ID:SGTC_539|Compound:4859-0462|FD-Score:5.04|P-value:2.72E-6 ID:SGTC_586|Compound:0957-0265|FD-Score:3.75|P-value:3.60E-4 ID:SGTC_753|Compound:1322-0200|FD-Score:-3.62|P-value:5.99E-4 ID:SGTC_818|Compound:4072-2707|FD-Score:4.68|P-value:1.22E-5 ID:SGTC_823|Compound:0417-1665|FD-Score:3.58|P-value:6.19E-4 ID:SGTC_839|Compound:0122-0028|FD-Score:3.57|P-value:6.24E-4 ID:SGTC_923|Compound:1900-2434|FD-Score:3.58|P-value:6.21E-4 ID:SGTC_955|Compound:1216-0110|FD-Score:4.14|P-value:9.21E-5 ID:SGTC_1159|Compound:4092-0538|FD-Score:3.46|P-value:8.82E-4 ID:SGTC_1331|Compound:1472-0049|FD-Score:3.70|P-value:4.17E-4 ID:SGTC_1335|Compound:1486-0900|FD-Score:-3.49|P-value:8.87E-4 ID:SGTC_1469|Compound:k284-2957|FD-Score:-4.41|P-value:3.89E-5 ID:SGTC_1789|Compound:tunicamycin|FD-Score:3.75|P-value:3.51E-4 ID:SGTC_1928|Compound:4'-methoxychalcone|FD-Score:-3.69|P-value:4.83E-4 ID:SGTC_1937|Compound:st074714|FD-Score:3.68|P-value:4.49E-4 ID:SGTC_1940|Compound:st075990|FD-Score:-3.56|P-value:7.23E-4 ID:SGTC_2010|Compound:4012422|FD-Score:-3.69|P-value:4.79E-4 ID:SGTC_2116|Compound:5511452|FD-Score:3.55|P-value:6.71E-4 ID:SGTC_2255|Compound:7941087|FD-Score:-3.88|P-value:2.53E-4 ID:SGTC_2302|Compound:7771753|FD-Score:3.64|P-value:5.01E-4 ID:SGTC_2383|Compound:9036125|FD-Score:4.12|P-value:9.88E-5 ID:SGTC_2419|Compound:paclitaxel|FD-Score:3.46|P-value:8.87E-4 ID:SGTC_2487|Compound:5918373|FD-Score:4.42|P-value:3.27E-5 ID:SGTC_2606|Compound:farnesol|FD-Score:3.81|P-value:2.90E-4 ID:SGTC_2781|Compound:5567276|FD-Score:3.49|P-value:8.03E-4 ID:SGTC_2901|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:3.43|P-value:9.72E-4 ID:SGTC_3080|Compound:9117754|FD-Score:3.51|P-value:7.62E-4 ID:SGTC_3097|Compound:9117246|FD-Score:4.73|P-value:9.93E-6 ID:SGTC_3104|Compound:9120930|FD-Score:3.65|P-value:4.95E-4 ID:SGTC_3162|Compound:9099653|FD-Score:-3.85|P-value:2.87E-4

Top fitness defect scores for YBR038W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_539 4859-0462 5.04 2.5 µM 2.72E-6 RNA pol III & RNase P/MRP 12 2.31E-7 0.15
2 SGTC_3097 9117246 4.73 49.5 µM 9.93E-6 1 1.12E-6 1.19
3 SGTC_818 4072-2707 4.68 53.5 µM 1.22E-5 4 1.44E-6 0.16
4 SGTC_2487 5918373 4.42 44.0 µM 3.27E-5 3 4.82E-6 0.30
5 SGTC_531 3788-1495 4.40 174.0 µM 3.64E-5 unfolded protein response 6 5.51E-6 0.15
6 SGTC_955 1216-0110 4.14 33.0 µM 9.21E-5 sphingolipid biosynthesis & PDR1 5 1.71E-5 0.39
7 SGTC_2383 9036125 4.12 200.0 µM 9.88E-5 2 1.86E-5 0.63
8 SGTC_528 3492-0019 3.97 172.0 µM 1.68E-4 calcium & mitochondrial duress 19 3.56E-5 0.01
9 SGTC_82 0054-0107 3.90 293.8 µM 2.13E-4 5 4.76E-5 0.13
10 SGTC_34 0469-0683 3.90 81.8 µM 2.17E-4 SWF1 & branched chain AA biosynthesis 5 4.85E-5 0.11
11 SGTC_2606 farnesol 3.81 9.8 µM 2.90E-4 12 6.92E-5 0.14
12 SGTC_1789 tunicamycin 3.75 25.0 nM 3.51E-4 unfolded protein response 17 8.73E-5 0.19
13 SGTC_586 0957-0265 3.75 8.2 µM 3.60E-4 cell wall 38 9.01E-5 0.04
14 SGTC_1331 1472-0049 3.70 76.2 µM 4.17E-4 6 1.08E-4 0.05
15 SGTC_1937 st074714 3.68 47.2 µM 4.49E-4 3 1.18E-4 0.20
16 SGTC_3104 9120930 3.65 49.5 µM 4.95E-4 4 1.33E-4 0.17
17 SGTC_2302 7771753 3.64 200.0 µM 5.01E-4 9 1.35E-4 0.03
18 SGTC_823 0417-1665 3.58 178.0 µM 6.19E-4 6 1.74E-4 0.10
19 SGTC_923 1900-2434 3.58 73.3 µM 6.21E-4 RNA processing & uracil transport 17 1.75E-4 0.09
20 SGTC_839 0122-0028 3.57 230.0 µM 6.24E-4 RSC complex & mRNA processing 42 1.76E-4 0.06

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.134 7.10E-15 YDR475C JIP4 hom Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence
0.095 3.18E-8 YAL032C PRP45 het Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene
0.088 3.84E-7 YOR086C TCB1 hom Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact
0.086 5.68E-7 YPL012W RRP12 het Protein required for export of the ribosomal subunits; associates with the RNA components of the pre-ribosomes; has a role in nuclear import in association with Pse1p; contains HEAT-repeats
0.084 9.91E-7 YGL203C KEX1 hom Protease involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins
0.084 1.14E-6 YDL071C_d YDL071C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF BDF2/YDL070W
0.084 1.25E-6 YBR131W CCZ1 hom Protein involved in vacuolar assembly, essential for autophagy and the cytoplasm-to-vacuole pathway
0.081 2.47E-6 YLR431C ATG23 hom Peripheral membrane protein required for the cytoplasm-to-vacuole targeting (Cvt) pathway and efficient macroautophagy; cycles between the phagophore assembly site (PAS) and non-PAS locations; forms a complex with Atg9p and Atg27p
0.080 3.35E-6 YOL050C_d YOL050C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
0.079 4.60E-6 YBR167C POP7 het Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
0.077 7.72E-6 YDR380W ARO10 hom Phenylpyruvate decarboxylase, catalyzes decarboxylation of phenylpyruvate to phenylacetaldehyde, which is the first specific step in the Ehrlich pathway
0.071 3.68E-5 YKL094W YJU3 hom Monoglyceride lipase (MGL), functional ortholog of mammalian MGL, localizes to lipid particles and membranes, also member of the eukaryotic serine hydrolase family
0.069 5.66E-5 YDR093W DNF2 hom Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase
0.069 6.33E-5 YLR404W FLD1 hom Seipin protein involved in lipid droplet morphology, number, and size; proposed to be involved in lipid metabolism; related to the human BSCL2 which is associated with lipodystrophy
0.068 7.35E-5 YIL099W SGA1 hom Intracellular sporulation-specific glucoamylase involved in glycogen degradation; induced during starvation of a/a diploids late in sporulation, but dispensable for sporulation