YBR060C / ORC2

Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; interacts with Spp1p and with trimethylated histone H3; phosphorylated by Cdc28p

Zygosity: Heterozygous strain
fixedexpanded
Profile for YBR060C / ORC2

Click on Significant Values for Screen Details ID:SGTC_190|Compound:3970-0790|FD-Score:-2.90|P-value:9.64E-4 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:3.63|P-value:6.85E-5 ID:SGTC_230|Compound:MPP+|FD-Score:4.73|P-value:3.18E-7 ID:SGTC_264|Compound:promethazine|FD-Score:5.53|P-value:2.78E-9 ID:SGTC_320|Compound:4092-0846|FD-Score:3.05|P-value:6.81E-4 ID:SGTC_333|Compound:3486-0131|FD-Score:-3.89|P-value:1.61E-5 ID:SGTC_422|Compound:cantharidin|FD-Score:3.22|P-value:3.64E-4 ID:SGTC_574|Compound:1315-0397|FD-Score:-3.27|P-value:2.31E-4 ID:SGTC_644|Compound:1498-1197|FD-Score:-3.20|P-value:3.10E-4 ID:SGTC_655|Compound:k060-0012|FD-Score:4.69|P-value:3.92E-7 ID:SGTC_658|Compound:3253-0951|FD-Score:3.11|P-value:5.50E-4 ID:SGTC_660|Compound:4031-1742|FD-Score:3.88|P-value:2.25E-5 ID:SGTC_767|Compound:0929-0023|FD-Score:3.03|P-value:7.28E-4 ID:SGTC_890|Compound:0929-0006|FD-Score:3.06|P-value:6.69E-4 ID:SGTC_893|Compound:0958-0369|FD-Score:4.05|P-value:1.04E-5 ID:SGTC_900|Compound:1112-0045|FD-Score:3.05|P-value:6.99E-4 ID:SGTC_903|Compound:1134-0042|FD-Score:3.52|P-value:1.10E-4 ID:SGTC_922|Compound:1866-0035|FD-Score:3.23|P-value:3.43E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:3.28|P-value:2.85E-4 ID:SGTC_1279|Compound:0874-0590|FD-Score:3.10|P-value:5.68E-4 ID:SGTC_1285|Compound:0929-0069|FD-Score:4.86|P-value:1.51E-7 ID:SGTC_1287|Compound:0971-0001|FD-Score:2.98|P-value:8.84E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:5.94|P-value:1.89E-10 ID:SGTC_1334|Compound:1486-0610|FD-Score:2.98|P-value:8.86E-4 ID:SGTC_1335|Compound:1486-0900|FD-Score:2.97|P-value:9.21E-4 ID:SGTC_1487|Compound:3970-0795|FD-Score:3.08|P-value:6.24E-4 ID:SGTC_1748|Compound:st037455|FD-Score:3.16|P-value:4.49E-4 ID:SGTC_1850|Compound:st056238|FD-Score:-3.72|P-value:3.52E-5 ID:SGTC_1979|Compound:st071809|FD-Score:-3.43|P-value:1.24E-4 ID:SGTC_1981|Compound:st071826|FD-Score:-3.35|P-value:1.70E-4 ID:SGTC_1994|Compound:st072947|FD-Score:-3.43|P-value:1.21E-4 ID:SGTC_2006|Compound:4011487|FD-Score:-3.26|P-value:2.41E-4 ID:SGTC_2012|Compound:4023501|FD-Score:4.48|P-value:1.19E-6 ID:SGTC_2079|Compound:5212404|FD-Score:3.09|P-value:6.04E-4 ID:SGTC_2088|Compound:5278280|FD-Score:4.86|P-value:1.56E-7 ID:SGTC_2245|Compound:6637752|FD-Score:3.45|P-value:1.46E-4 ID:SGTC_2303|Compound:7774051|FD-Score:-3.33|P-value:1.84E-4 ID:SGTC_2425|Compound:5628681|FD-Score:3.02|P-value:7.69E-4 ID:SGTC_2579|Compound:2',5'-dihydroxy-4-methoxychalcone|FD-Score:3.80|P-value:3.18E-5 ID:SGTC_2613|Compound:3',4'-dimethoxydalbergione|FD-Score:-4.64|P-value:3.83E-7 ID:SGTC_2696|Compound:st077691|FD-Score:4.02|P-value:1.17E-5 ID:SGTC_2808|Compound:7946468|FD-Score:-4.49|P-value:8.55E-7 ID:SGTC_2813|Compound:7971799|FD-Score:5.65|P-value:1.30E-9 ID:SGTC_2814|Compound:7977482|FD-Score:-3.05|P-value:5.57E-4 ID:SGTC_2846|Compound:9008642|FD-Score:-2.95|P-value:7.96E-4 ID:SGTC_2919|Compound:7978188|FD-Score:6.01|P-value:1.21E-10 ID:SGTC_2965|Compound:9082772|FD-Score:-3.22|P-value:2.83E-4 ID:SGTC_3014|Compound:9081533|FD-Score:3.03|P-value:7.49E-4 ID:SGTC_3030|Compound:9087619|FD-Score:3.58|P-value:8.60E-5 ID:SGTC_3055|Compound:9094636|FD-Score:-3.79|P-value:2.57E-5 ID:SGTC_3101|Compound:9120531|FD-Score:4.92|P-value:1.12E-7 ID:SGTC_3108|Compound:9121785|FD-Score:5.41|P-value:5.95E-9 ID:SGTC_3114|Compound:9122766|FD-Score:3.14|P-value:4.83E-4 ID:SGTC_3346|Compound:9158254|FD-Score:3.68|P-value:5.57E-5 ID:SGTC_190|Compound:3970-0790|FD-Score:-2.90|P-value:9.64E-4 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:3.63|P-value:6.85E-5 ID:SGTC_230|Compound:MPP+|FD-Score:4.73|P-value:3.18E-7 ID:SGTC_264|Compound:promethazine|FD-Score:5.53|P-value:2.78E-9 ID:SGTC_320|Compound:4092-0846|FD-Score:3.05|P-value:6.81E-4 ID:SGTC_333|Compound:3486-0131|FD-Score:-3.89|P-value:1.61E-5 ID:SGTC_422|Compound:cantharidin|FD-Score:3.22|P-value:3.64E-4 ID:SGTC_574|Compound:1315-0397|FD-Score:-3.27|P-value:2.31E-4 ID:SGTC_644|Compound:1498-1197|FD-Score:-3.20|P-value:3.10E-4 ID:SGTC_655|Compound:k060-0012|FD-Score:4.69|P-value:3.92E-7 ID:SGTC_658|Compound:3253-0951|FD-Score:3.11|P-value:5.50E-4 ID:SGTC_660|Compound:4031-1742|FD-Score:3.88|P-value:2.25E-5 ID:SGTC_767|Compound:0929-0023|FD-Score:3.03|P-value:7.28E-4 ID:SGTC_890|Compound:0929-0006|FD-Score:3.06|P-value:6.69E-4 ID:SGTC_893|Compound:0958-0369|FD-Score:4.05|P-value:1.04E-5 ID:SGTC_900|Compound:1112-0045|FD-Score:3.05|P-value:6.99E-4 ID:SGTC_903|Compound:1134-0042|FD-Score:3.52|P-value:1.10E-4 ID:SGTC_922|Compound:1866-0035|FD-Score:3.23|P-value:3.43E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:3.28|P-value:2.85E-4 ID:SGTC_1279|Compound:0874-0590|FD-Score:3.10|P-value:5.68E-4 ID:SGTC_1285|Compound:0929-0069|FD-Score:4.86|P-value:1.51E-7 ID:SGTC_1287|Compound:0971-0001|FD-Score:2.98|P-value:8.84E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:5.94|P-value:1.89E-10 ID:SGTC_1334|Compound:1486-0610|FD-Score:2.98|P-value:8.86E-4 ID:SGTC_1335|Compound:1486-0900|FD-Score:2.97|P-value:9.21E-4 ID:SGTC_1487|Compound:3970-0795|FD-Score:3.08|P-value:6.24E-4 ID:SGTC_1748|Compound:st037455|FD-Score:3.16|P-value:4.49E-4 ID:SGTC_1850|Compound:st056238|FD-Score:-3.72|P-value:3.52E-5 ID:SGTC_1979|Compound:st071809|FD-Score:-3.43|P-value:1.24E-4 ID:SGTC_1981|Compound:st071826|FD-Score:-3.35|P-value:1.70E-4 ID:SGTC_1994|Compound:st072947|FD-Score:-3.43|P-value:1.21E-4 ID:SGTC_2006|Compound:4011487|FD-Score:-3.26|P-value:2.41E-4 ID:SGTC_2012|Compound:4023501|FD-Score:4.48|P-value:1.19E-6 ID:SGTC_2079|Compound:5212404|FD-Score:3.09|P-value:6.04E-4 ID:SGTC_2088|Compound:5278280|FD-Score:4.86|P-value:1.56E-7 ID:SGTC_2245|Compound:6637752|FD-Score:3.45|P-value:1.46E-4 ID:SGTC_2303|Compound:7774051|FD-Score:-3.33|P-value:1.84E-4 ID:SGTC_2425|Compound:5628681|FD-Score:3.02|P-value:7.69E-4 ID:SGTC_2579|Compound:2',5'-dihydroxy-4-methoxychalcone|FD-Score:3.80|P-value:3.18E-5 ID:SGTC_2613|Compound:3',4'-dimethoxydalbergione|FD-Score:-4.64|P-value:3.83E-7 ID:SGTC_2696|Compound:st077691|FD-Score:4.02|P-value:1.17E-5 ID:SGTC_2808|Compound:7946468|FD-Score:-4.49|P-value:8.55E-7 ID:SGTC_2813|Compound:7971799|FD-Score:5.65|P-value:1.30E-9 ID:SGTC_2814|Compound:7977482|FD-Score:-3.05|P-value:5.57E-4 ID:SGTC_2846|Compound:9008642|FD-Score:-2.95|P-value:7.96E-4 ID:SGTC_2919|Compound:7978188|FD-Score:6.01|P-value:1.21E-10 ID:SGTC_2965|Compound:9082772|FD-Score:-3.22|P-value:2.83E-4 ID:SGTC_3014|Compound:9081533|FD-Score:3.03|P-value:7.49E-4 ID:SGTC_3030|Compound:9087619|FD-Score:3.58|P-value:8.60E-5 ID:SGTC_3055|Compound:9094636|FD-Score:-3.79|P-value:2.57E-5 ID:SGTC_3101|Compound:9120531|FD-Score:4.92|P-value:1.12E-7 ID:SGTC_3108|Compound:9121785|FD-Score:5.41|P-value:5.95E-9 ID:SGTC_3114|Compound:9122766|FD-Score:3.14|P-value:4.83E-4 ID:SGTC_3346|Compound:9158254|FD-Score:3.68|P-value:5.57E-5

Top fitness defect scores for YBR060C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2919 7978188 6.01 46.4 µM 1.21E-10 1 9.47E-10 1.87
2 SGTC_1290 0986-0249 5.94 10.2 µM 1.89E-10 DNA intercalators 2 1.42E-9 0.76
3 SGTC_2813 7971799 5.65 71.4 µM 1.30E-9 2 8.01E-9 0.99
4 SGTC_264 promethazine 5.53 12.5 mM 2.78E-9 DNA intercalators 12 1.58E-8 0.22
5 SGTC_3108 9121785 5.41 49.5 µM 5.95E-9 1 3.13E-8 0.51
6 SGTC_3101 9120531 4.92 49.5 µM 1.12E-7 4 4.37E-7 0.76
7 SGTC_1285 0929-0069 4.86 65.5 µM 1.51E-7 2 5.73E-7 0.98
8 SGTC_2088 5278280 4.86 127.4 µM 1.56E-7 2 5.91E-7 0.86
9 SGTC_230 MPP+ 4.73 141.7 µM 3.18E-7 3 1.12E-6 0.15
10 SGTC_655 k060-0012 4.69 42.0 µM 3.92E-7 1 1.35E-6 0.40
11 SGTC_2012 4023501 4.48 200.0 µM 1.19E-6 7 3.67E-6 0.26
12 SGTC_893 0958-0369 4.05 48.9 µM 1.04E-5 3 2.59E-5 0.27
13 SGTC_2696 st077691 4.02 74.3 µM 1.17E-5 11 2.87E-5 0.03
14 SGTC_660 4031-1742 3.88 102.0 µM 2.25E-5 redox potentiating 14 5.20E-5 0.01
15 SGTC_2579 2',5'-dihydroxy-4-methoxychalcone 3.80 13.2 µM 3.18E-5 10 7.10E-5 0.25
16 SGTC_3346 9158254 3.68 43.6 µM 5.57E-5 17 1.18E-4 0.08
17 SGTC_219 mitoxanthrone 3.63 164.4 µM 6.85E-5 DNA intercalators 16 1.42E-4 0.03
18 SGTC_3030 9087619 3.58 49.5 µM 8.60E-5 10 1.74E-4 0.05
19 SGTC_903 1134-0042 3.52 114.0 µM 1.10E-4 10 2.19E-4 0.05
20 SGTC_2245 6637752 3.45 200.0 µM 1.46E-4 7 2.81E-4 0.03

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.185 3.10E-27 YHL039W EFM1 hom Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.171 2.33E-23 YMR168C CEP3 het Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
0.142 1.41E-16 YMR166C_p YMR166C_p hom Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene
0.142 1.57E-16 YOR133W EFT1 hom Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication
0.138 9.62E-16 YPL157W TGS1 hom Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs
0.137 2.03E-15 YMR289W ABZ2 hom Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis
0.126 2.21E-13 YJR135C MCM22 hom Outer kinetochore protein and component of the Ctf3 subcomplex; binds to centromeric DNA in a Ctf19p-dependent manner; involved in chromosome segregation and minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-K and fission yeast sim4
0.122 1.23E-12 YKL067W YNK1 hom Nucleoside diphosphate kinase; catalyzes the transfer of gamma phosphates from nucleoside triphosphates, usually ATP, to nucleoside diphosphates by a mechanism that involves formation of an autophosphorylated enzyme intermediate; protein abundance increases in response to DNA replication stress
0.111 1.14E-10 YML118W NGL3 hom 3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication
0.111 1.37E-10 YDL223C HBT1 hom Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis
0.103 2.67E-9 YGL205W POX1 hom Fatty-acyl coenzyme A oxidase, involved in the fatty acid beta-oxidation pathway; localized to the peroxisomal matrix
0.102 4.05E-9 YNL009W IDP3 hom Peroxisomal NADP-dependent isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate with the formation of NADP(H+), required for growth on unsaturated fatty acids; IDP3 has a paralog, IDP2, that arose from the whole genome duplication
0.099 9.64E-9 YBR286W APE3 hom Vacuolar aminopeptidase Y, processed to mature form by Prb1p
0.097 1.67E-8 YDR164C SEC1 het Sm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function
0.095 4.26E-8 YDR339C FCF1 het Putative PINc domain nuclease required for early cleavages of 35S pre-rRNA and maturation of 18S rRNA; component of the SSU (small subunit) processome involved in 40S ribosomal subunit biogenesis; copurifies with Faf1p