YBR061C / TRM7

2'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of the tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR061C / TRM7

Click on Significant Values for Screen Details ID:SGTC_55|Compound:0767-0285|FD-Score:-3.17|P-value:6.63E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:6.94|P-value:1.07E-12 ID:SGTC_64|Compound:000l-0018|FD-Score:-6.67|P-value:7.42E-12 ID:SGTC_142|Compound:0335-0849|FD-Score:-3.24|P-value:5.14E-4 ID:SGTC_178|Compound:4092-0806|FD-Score:4.02|P-value:2.33E-5 ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:3.72|P-value:8.39E-5 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:3.38|P-value:3.11E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:3.75|P-value:7.44E-5 ID:SGTC_312|Compound:4092-0653|FD-Score:7.89|P-value:7.33E-16 ID:SGTC_320|Compound:4092-0846|FD-Score:7.02|P-value:6.15E-13 ID:SGTC_329|Compound:k048-0121|FD-Score:-3.68|P-value:9.99E-5 ID:SGTC_332|Compound:4112-0506|FD-Score:4.09|P-value:1.78E-5 ID:SGTC_346|Compound:4513-0042|FD-Score:4.46|P-value:3.15E-6 ID:SGTC_408|Compound:cantharidin|FD-Score:3.14|P-value:7.47E-4 ID:SGTC_422|Compound:cantharidin|FD-Score:3.42|P-value:2.64E-4 ID:SGTC_444|Compound:curcumin|FD-Score:-3.11|P-value:8.14E-4 ID:SGTC_451|Compound:chloral hydrate|FD-Score:4.59|P-value:1.71E-6 ID:SGTC_455|Compound:paf C-16|FD-Score:3.77|P-value:6.75E-5 ID:SGTC_461|Compound:N-acetylfarnesylcysteine|FD-Score:3.11|P-value:8.25E-4 ID:SGTC_473|Compound:2-arachidonoylglycerol|FD-Score:-3.34|P-value:3.69E-4 ID:SGTC_492|Compound:tmb-8|FD-Score:3.24|P-value:5.25E-4 ID:SGTC_597|Compound:k018-0003|FD-Score:8.51|P-value:3.69E-18 ID:SGTC_608|Compound:k048-0125|FD-Score:12.80|P-value:2.16E-38 ID:SGTC_659|Compound:k018-0002|FD-Score:11.30|P-value:1.94E-30 ID:SGTC_678|Compound:1218-2179|FD-Score:5.32|P-value:3.68E-8 ID:SGTC_712|Compound:r015-0009|FD-Score:9.73|P-value:3.48E-23 ID:SGTC_782|Compound:0083-0095|FD-Score:3.61|P-value:1.31E-4 ID:SGTC_905|Compound:2-aminofluorene|FD-Score:3.08|P-value:9.25E-4 ID:SGTC_1008|Compound:4106-0037|FD-Score:4.70|P-value:1.01E-6 ID:SGTC_1014|Compound:4130-3005|FD-Score:-3.28|P-value:4.59E-4 ID:SGTC_1047|Compound:2043-3044|FD-Score:-3.52|P-value:1.81E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:5.68|P-value:4.54E-9 ID:SGTC_1084|Compound:voriconazole|FD-Score:4.08|P-value:1.79E-5 ID:SGTC_1129|Compound:3453-1384|FD-Score:-3.25|P-value:5.00E-4 ID:SGTC_1133|Compound:3473-0247|FD-Score:5.26|P-value:5.17E-8 ID:SGTC_1277|Compound:0873-0036|FD-Score:3.09|P-value:8.82E-4 ID:SGTC_1286|Compound:0929-0070|FD-Score:3.06|P-value:9.85E-4 ID:SGTC_1287|Compound:0971-0001|FD-Score:4.70|P-value:9.72E-7 ID:SGTC_1304|Compound:1165-0502|FD-Score:3.57|P-value:1.54E-4 ID:SGTC_1360|Compound:1598-0022|FD-Score:3.26|P-value:4.79E-4 ID:SGTC_1416|Compound:3966-0321|FD-Score:3.36|P-value:3.37E-4 ID:SGTC_1507|Compound:4075-3230|FD-Score:5.47|P-value:1.51E-8 ID:SGTC_1568|Compound:digitonin|FD-Score:3.39|P-value:3.04E-4 ID:SGTC_1777|Compound:st045864|FD-Score:3.20|P-value:6.00E-4 ID:SGTC_1783|Compound:st039459|FD-Score:5.60|P-value:7.40E-9 ID:SGTC_1788|Compound:fluconazole|FD-Score:5.40|P-value:2.33E-8 ID:SGTC_1789|Compound:tunicamycin|FD-Score:-4.11|P-value:1.60E-5 ID:SGTC_1799|Compound:st045822|FD-Score:3.17|P-value:6.59E-4 ID:SGTC_1800|Compound:st050079|FD-Score:3.21|P-value:5.84E-4 ID:SGTC_1877|Compound:wortmannin|FD-Score:4.17|P-value:1.25E-5 ID:SGTC_1915|Compound:st067105|FD-Score:6.51|P-value:2.29E-11 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-4.77|P-value:6.80E-7 ID:SGTC_1934|Compound:st074720|FD-Score:4.01|P-value:2.48E-5 ID:SGTC_2002|Compound:4023503|FD-Score:3.80|P-value:5.96E-5 ID:SGTC_2011|Compound:4012611|FD-Score:4.28|P-value:7.28E-6 ID:SGTC_2033|Compound:5192550|FD-Score:3.24|P-value:5.15E-4 ID:SGTC_2207|Compound:7258803|FD-Score:3.43|P-value:2.56E-4 ID:SGTC_2245|Compound:6637752|FD-Score:5.54|P-value:1.04E-8 ID:SGTC_2250|Compound:alverine citrate|FD-Score:4.82|P-value:5.46E-7 ID:SGTC_2304|Compound:7443016|FD-Score:3.18|P-value:6.51E-4 ID:SGTC_2422|Compound:chlorphenesin carbamate|FD-Score:3.76|P-value:7.17E-5 ID:SGTC_2481|Compound:5478948|FD-Score:3.56|P-value:1.57E-4 ID:SGTC_2486|Compound:5283202|FD-Score:-3.50|P-value:1.96E-4 ID:SGTC_2489|Compound:cantharidin|FD-Score:6.04|P-value:4.95E-10 ID:SGTC_2504|Compound:purpurogallin|FD-Score:-3.41|P-value:2.79E-4 ID:SGTC_2517|Compound:totarol|FD-Score:4.28|P-value:7.43E-6 ID:SGTC_2625|Compound:euphol|FD-Score:3.14|P-value:7.53E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:6.27|P-value:1.12E-10 ID:SGTC_2730|Compound:imipramine|FD-Score:-3.52|P-value:1.81E-4 ID:SGTC_2739|Compound:tacrine|FD-Score:-4.28|P-value:7.39E-6 ID:SGTC_2785|Compound:7756463|FD-Score:3.23|P-value:5.47E-4 ID:SGTC_2791|Compound:5663188|FD-Score:3.64|P-value:1.16E-4 ID:SGTC_2809|Compound:7964133|FD-Score:3.16|P-value:6.99E-4 ID:SGTC_2815|Compound:7968870|FD-Score:4.26|P-value:8.06E-6 ID:SGTC_2978|Compound:9011784|FD-Score:3.11|P-value:8.23E-4 ID:SGTC_2985|Compound:9022375|FD-Score:-3.20|P-value:6.00E-4 ID:SGTC_2989|Compound:9073649|FD-Score:5.66|P-value:4.93E-9 ID:SGTC_2993|Compound:9054257|FD-Score:-4.45|P-value:3.40E-6 ID:SGTC_3003|Compound:9075612|FD-Score:4.07|P-value:1.89E-5 ID:SGTC_3019|Compound:9081971|FD-Score:4.01|P-value:2.52E-5 ID:SGTC_3020|Compound:9089140|FD-Score:7.54|P-value:1.14E-14 ID:SGTC_3178|Compound:9107506|FD-Score:3.12|P-value:7.82E-4 ID:SGTC_3191|Compound:9110352|FD-Score:-3.26|P-value:4.85E-4 ID:SGTC_3347|Compound:9188721|FD-Score:5.48|P-value:1.43E-8 ID:SGTC_55|Compound:0767-0285|FD-Score:-3.17|P-value:6.63E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:6.94|P-value:1.07E-12 ID:SGTC_64|Compound:000l-0018|FD-Score:-6.67|P-value:7.42E-12 ID:SGTC_142|Compound:0335-0849|FD-Score:-3.24|P-value:5.14E-4 ID:SGTC_178|Compound:4092-0806|FD-Score:4.02|P-value:2.33E-5 ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:3.72|P-value:8.39E-5 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:3.38|P-value:3.11E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:3.75|P-value:7.44E-5 ID:SGTC_312|Compound:4092-0653|FD-Score:7.89|P-value:7.33E-16 ID:SGTC_320|Compound:4092-0846|FD-Score:7.02|P-value:6.15E-13 ID:SGTC_329|Compound:k048-0121|FD-Score:-3.68|P-value:9.99E-5 ID:SGTC_332|Compound:4112-0506|FD-Score:4.09|P-value:1.78E-5 ID:SGTC_346|Compound:4513-0042|FD-Score:4.46|P-value:3.15E-6 ID:SGTC_408|Compound:cantharidin|FD-Score:3.14|P-value:7.47E-4 ID:SGTC_422|Compound:cantharidin|FD-Score:3.42|P-value:2.64E-4 ID:SGTC_444|Compound:curcumin|FD-Score:-3.11|P-value:8.14E-4 ID:SGTC_451|Compound:chloral hydrate|FD-Score:4.59|P-value:1.71E-6 ID:SGTC_455|Compound:paf C-16|FD-Score:3.77|P-value:6.75E-5 ID:SGTC_461|Compound:N-acetylfarnesylcysteine|FD-Score:3.11|P-value:8.25E-4 ID:SGTC_473|Compound:2-arachidonoylglycerol|FD-Score:-3.34|P-value:3.69E-4 ID:SGTC_492|Compound:tmb-8|FD-Score:3.24|P-value:5.25E-4 ID:SGTC_597|Compound:k018-0003|FD-Score:8.51|P-value:3.69E-18 ID:SGTC_608|Compound:k048-0125|FD-Score:12.80|P-value:2.16E-38 ID:SGTC_659|Compound:k018-0002|FD-Score:11.30|P-value:1.94E-30 ID:SGTC_678|Compound:1218-2179|FD-Score:5.32|P-value:3.68E-8 ID:SGTC_712|Compound:r015-0009|FD-Score:9.73|P-value:3.48E-23 ID:SGTC_782|Compound:0083-0095|FD-Score:3.61|P-value:1.31E-4 ID:SGTC_905|Compound:2-aminofluorene|FD-Score:3.08|P-value:9.25E-4 ID:SGTC_1008|Compound:4106-0037|FD-Score:4.70|P-value:1.01E-6 ID:SGTC_1014|Compound:4130-3005|FD-Score:-3.28|P-value:4.59E-4 ID:SGTC_1047|Compound:2043-3044|FD-Score:-3.52|P-value:1.81E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:5.68|P-value:4.54E-9 ID:SGTC_1084|Compound:voriconazole|FD-Score:4.08|P-value:1.79E-5 ID:SGTC_1129|Compound:3453-1384|FD-Score:-3.25|P-value:5.00E-4 ID:SGTC_1133|Compound:3473-0247|FD-Score:5.26|P-value:5.17E-8 ID:SGTC_1277|Compound:0873-0036|FD-Score:3.09|P-value:8.82E-4 ID:SGTC_1286|Compound:0929-0070|FD-Score:3.06|P-value:9.85E-4 ID:SGTC_1287|Compound:0971-0001|FD-Score:4.70|P-value:9.72E-7 ID:SGTC_1304|Compound:1165-0502|FD-Score:3.57|P-value:1.54E-4 ID:SGTC_1360|Compound:1598-0022|FD-Score:3.26|P-value:4.79E-4 ID:SGTC_1416|Compound:3966-0321|FD-Score:3.36|P-value:3.37E-4 ID:SGTC_1507|Compound:4075-3230|FD-Score:5.47|P-value:1.51E-8 ID:SGTC_1568|Compound:digitonin|FD-Score:3.39|P-value:3.04E-4 ID:SGTC_1777|Compound:st045864|FD-Score:3.20|P-value:6.00E-4 ID:SGTC_1783|Compound:st039459|FD-Score:5.60|P-value:7.40E-9 ID:SGTC_1788|Compound:fluconazole|FD-Score:5.40|P-value:2.33E-8 ID:SGTC_1789|Compound:tunicamycin|FD-Score:-4.11|P-value:1.60E-5 ID:SGTC_1799|Compound:st045822|FD-Score:3.17|P-value:6.59E-4 ID:SGTC_1800|Compound:st050079|FD-Score:3.21|P-value:5.84E-4 ID:SGTC_1877|Compound:wortmannin|FD-Score:4.17|P-value:1.25E-5 ID:SGTC_1915|Compound:st067105|FD-Score:6.51|P-value:2.29E-11 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-4.77|P-value:6.80E-7 ID:SGTC_1934|Compound:st074720|FD-Score:4.01|P-value:2.48E-5 ID:SGTC_2002|Compound:4023503|FD-Score:3.80|P-value:5.96E-5 ID:SGTC_2011|Compound:4012611|FD-Score:4.28|P-value:7.28E-6 ID:SGTC_2033|Compound:5192550|FD-Score:3.24|P-value:5.15E-4 ID:SGTC_2207|Compound:7258803|FD-Score:3.43|P-value:2.56E-4 ID:SGTC_2245|Compound:6637752|FD-Score:5.54|P-value:1.04E-8 ID:SGTC_2250|Compound:alverine citrate|FD-Score:4.82|P-value:5.46E-7 ID:SGTC_2304|Compound:7443016|FD-Score:3.18|P-value:6.51E-4 ID:SGTC_2422|Compound:chlorphenesin carbamate|FD-Score:3.76|P-value:7.17E-5 ID:SGTC_2481|Compound:5478948|FD-Score:3.56|P-value:1.57E-4 ID:SGTC_2486|Compound:5283202|FD-Score:-3.50|P-value:1.96E-4 ID:SGTC_2489|Compound:cantharidin|FD-Score:6.04|P-value:4.95E-10 ID:SGTC_2504|Compound:purpurogallin|FD-Score:-3.41|P-value:2.79E-4 ID:SGTC_2517|Compound:totarol|FD-Score:4.28|P-value:7.43E-6 ID:SGTC_2625|Compound:euphol|FD-Score:3.14|P-value:7.53E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:6.27|P-value:1.12E-10 ID:SGTC_2730|Compound:imipramine|FD-Score:-3.52|P-value:1.81E-4 ID:SGTC_2739|Compound:tacrine|FD-Score:-4.28|P-value:7.39E-6 ID:SGTC_2785|Compound:7756463|FD-Score:3.23|P-value:5.47E-4 ID:SGTC_2791|Compound:5663188|FD-Score:3.64|P-value:1.16E-4 ID:SGTC_2809|Compound:7964133|FD-Score:3.16|P-value:6.99E-4 ID:SGTC_2815|Compound:7968870|FD-Score:4.26|P-value:8.06E-6 ID:SGTC_2978|Compound:9011784|FD-Score:3.11|P-value:8.23E-4 ID:SGTC_2985|Compound:9022375|FD-Score:-3.20|P-value:6.00E-4 ID:SGTC_2989|Compound:9073649|FD-Score:5.66|P-value:4.93E-9 ID:SGTC_2993|Compound:9054257|FD-Score:-4.45|P-value:3.40E-6 ID:SGTC_3003|Compound:9075612|FD-Score:4.07|P-value:1.89E-5 ID:SGTC_3019|Compound:9081971|FD-Score:4.01|P-value:2.52E-5 ID:SGTC_3020|Compound:9089140|FD-Score:7.54|P-value:1.14E-14 ID:SGTC_3178|Compound:9107506|FD-Score:3.12|P-value:7.82E-4 ID:SGTC_3191|Compound:9110352|FD-Score:-3.26|P-value:4.85E-4 ID:SGTC_3347|Compound:9188721|FD-Score:5.48|P-value:1.43E-8

Top fitness defect scores for YBR061C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_608 k048-0125 12.80 159.0 µM 2.16E-38 2 1.55E-37
2 SGTC_659 k018-0002 11.30 107.0 µM 1.94E-30 1 9.11E-30
3 SGTC_712 r015-0009 9.73 80.6 µM 3.48E-23 2 1.11E-22
4 SGTC_597 k018-0003 8.51 82.8 µM 3.69E-18 RNA pol III & RNase P/MRP 1 8.93E-18
5 SGTC_312 4092-0653 7.89 6.8 µM 7.33E-16 ergosterol depletion effects on membrane 22 1.57E-15
6 SGTC_3020 9089140 7.54 49.5 µM 1.14E-14 2 2.29E-14
7 SGTC_320 4092-0846 7.02 5.3 µM 6.15E-13 ergosterol depletion effects on membrane 8 1.13E-12
8 SGTC_58 0957-0393 6.94 9.0 µM 1.07E-12 cell wall 165 1.94E-12
9 SGTC_1915 st067105 6.51 30.6 µM 2.29E-11 RNA pol III & RNase P/MRP 7 3.86E-11
10 SGTC_2651 1327-0036 6.27 5.0 µM 1.12E-10 65 1.81E-10
11 SGTC_2489 cantharidin 6.04 73.4 µM 4.95E-10 cell wall signaling 9 7.78E-10
12 SGTC_1083 didemethylchlorpromazine sulfoxide 5.68 707.4 nM 4.54E-9 redox potentiating 32 6.78E-9
13 SGTC_2989 9073649 5.66 71.4 µM 4.93E-9 10 7.35E-9
14 SGTC_1783 st039459 5.60 87.5 µM 7.40E-9 Golgi 6 1.09E-8
15 SGTC_2245 6637752 5.54 200.0 µM 1.04E-8 10 1.53E-8
16 SGTC_3347 9188721 5.48 44.7 µM 1.43E-8 8 2.08E-8
17 SGTC_1507 4075-3230 5.47 155.0 µM 1.51E-8 5 2.20E-8
18 SGTC_1788 fluconazole 5.40 20.0 µM 2.33E-8 azole & statin 38 3.35E-8
19 SGTC_678 1218-2179 5.32 85.9 µM 3.68E-8 RNA pol III & RNase P/MRP 2 5.24E-8
20 SGTC_1133 3473-0247 5.26 50.0 µM 5.17E-8 RSC complex & mRNA processing 18 7.30E-8

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.319 6.56E-80 YPL183C RTT10 hom WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6
0.248 4.32E-48 YLR060W FRS1 het Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control
0.162 3.24E-21 YIL068C SEC6 het Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p
0.152 8.82E-19 YHR209W CRG1 hom S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin
0.152 9.42E-19 YFL022C FRS2 het Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar
0.127 1.47E-13 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.124 4.91E-13 YIL160C POT1 hom 3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids
0.122 1.20E-12 YOR116C RPO31 het RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
0.116 1.46E-11 YJR074W MOG1 hom Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p
0.111 1.05E-10 YDR407C TRS120 het One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic
0.111 1.09E-10 YML103C NUP188 hom Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC organization and nucleocytoplasmic transport; homologous to human NUP188
0.111 1.10E-10 YBR103W SIF2 hom WD40 repeat-containing subunit of the Set3C histone deacetylase complex, which represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus
0.110 1.64E-10 YHR007C ERG11 het Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
0.110 1.69E-10 YBR102C EXO84 het Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate
0.110 1.97E-10 YDR435C PPM1 hom Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits