YBR070C / ALG14

Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases

Zygosity: Heterozygous strain
fixedexpanded
Profile for YBR070C / ALG14

Click on Significant Values for Screen Details ID:SGTC_68|Compound:0986-0284|FD-Score:-4.74|P-value:1.89E-6 ID:SGTC_148|Compound:3253-0744|FD-Score:-4.43|P-value:8.03E-6 ID:SGTC_152|Compound:4469-0003|FD-Score:-3.26|P-value:7.26E-4 ID:SGTC_251|Compound:melphalan|FD-Score:3.21|P-value:9.25E-4 ID:SGTC_260|Compound:nsc-65238|FD-Score:3.70|P-value:1.60E-4 ID:SGTC_371|Compound:0195-0006|FD-Score:-4.08|P-value:3.53E-5 ID:SGTC_738|Compound:1315-0003|FD-Score:4.54|P-value:5.17E-6 ID:SGTC_953|Compound:1188-0045|FD-Score:3.50|P-value:3.42E-4 ID:SGTC_964|Compound:1287-0107|FD-Score:4.84|P-value:1.25E-6 ID:SGTC_993|Compound:3909-8757|FD-Score:4.07|P-value:3.89E-5 ID:SGTC_1100|Compound:amiodarone|FD-Score:3.43|P-value:4.24E-4 ID:SGTC_1105|Compound:epirubicin|FD-Score:-4.05|P-value:3.87E-5 ID:SGTC_1388|Compound:3346-4487|FD-Score:3.20|P-value:9.39E-4 ID:SGTC_1402|Compound:3909-7673|FD-Score:3.52|P-value:3.11E-4 ID:SGTC_1428|Compound:4049-0204|FD-Score:3.52|P-value:3.18E-4 ID:SGTC_1457|Compound:k015-0032|FD-Score:-3.17|P-value:9.83E-4 ID:SGTC_1632|Compound:st005650|FD-Score:3.24|P-value:8.13E-4 ID:SGTC_1657|Compound:st012939|FD-Score:3.24|P-value:8.37E-4 ID:SGTC_1784|Compound:st039334|FD-Score:6.16|P-value:1.05E-9 ID:SGTC_2002|Compound:4023503|FD-Score:-5.48|P-value:4.53E-8 ID:SGTC_2012|Compound:4023501|FD-Score:-4.45|P-value:7.28E-6 ID:SGTC_2023|Compound:5144124|FD-Score:3.34|P-value:5.91E-4 ID:SGTC_2102|Compound:5377816|FD-Score:4.18|P-value:2.45E-5 ID:SGTC_2206|Compound:7254556|FD-Score:3.63|P-value:2.12E-4 ID:SGTC_2223|Compound:6903751|FD-Score:3.96|P-value:5.86E-5 ID:SGTC_2274|Compound:7947094|FD-Score:-3.95|P-value:5.96E-5 ID:SGTC_2347|Compound:9049783|FD-Score:-3.21|P-value:8.61E-4 ID:SGTC_2469|Compound:5655019|FD-Score:4.25|P-value:1.79E-5 ID:SGTC_2488|Compound:5268135|FD-Score:3.37|P-value:5.23E-4 ID:SGTC_2561|Compound:acetosyringone|FD-Score:3.24|P-value:8.23E-4 ID:SGTC_2564|Compound:betulinic acid|FD-Score:13.70|P-value:8.08E-41 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:4.82|P-value:1.43E-6 ID:SGTC_2578|Compound:7,8-dihydroxyflavone|FD-Score:-3.48|P-value:3.48E-4 ID:SGTC_2593|Compound:nobiletin|FD-Score:3.91|P-value:7.38E-5 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:4.44|P-value:8.07E-6 ID:SGTC_2701|Compound:st077767|FD-Score:-3.48|P-value:3.43E-4 ID:SGTC_2703|Compound:st077852|FD-Score:5.91|P-value:4.61E-9 ID:SGTC_2750|Compound:bromebric acid|FD-Score:3.71|P-value:1.55E-4 ID:SGTC_2762|Compound:leflunomide|FD-Score:3.84|P-value:9.51E-5 ID:SGTC_2788|Compound:5282734|FD-Score:3.58|P-value:2.57E-4 ID:SGTC_2989|Compound:9073649|FD-Score:-3.27|P-value:7.03E-4 ID:SGTC_3044|Compound:9093193|FD-Score:-3.39|P-value:4.69E-4 ID:SGTC_3050|Compound:9093513|FD-Score:3.41|P-value:4.56E-4 ID:SGTC_3054|Compound:9094397|FD-Score:4.42|P-value:8.59E-6 ID:SGTC_3098|Compound:9119039|FD-Score:3.35|P-value:5.62E-4 ID:SGTC_3147|Compound:9097027|FD-Score:3.65|P-value:1.92E-4 ID:SGTC_3148|Compound:9097893|FD-Score:3.48|P-value:3.63E-4 ID:SGTC_3282|Compound:9108498|FD-Score:-3.53|P-value:2.84E-4 ID:SGTC_3292|Compound:9120825|FD-Score:-3.37|P-value:5.03E-4 ID:SGTC_68|Compound:0986-0284|FD-Score:-4.74|P-value:1.89E-6 ID:SGTC_148|Compound:3253-0744|FD-Score:-4.43|P-value:8.03E-6 ID:SGTC_152|Compound:4469-0003|FD-Score:-3.26|P-value:7.26E-4 ID:SGTC_251|Compound:melphalan|FD-Score:3.21|P-value:9.25E-4 ID:SGTC_260|Compound:nsc-65238|FD-Score:3.70|P-value:1.60E-4 ID:SGTC_371|Compound:0195-0006|FD-Score:-4.08|P-value:3.53E-5 ID:SGTC_738|Compound:1315-0003|FD-Score:4.54|P-value:5.17E-6 ID:SGTC_953|Compound:1188-0045|FD-Score:3.50|P-value:3.42E-4 ID:SGTC_964|Compound:1287-0107|FD-Score:4.84|P-value:1.25E-6 ID:SGTC_993|Compound:3909-8757|FD-Score:4.07|P-value:3.89E-5 ID:SGTC_1100|Compound:amiodarone|FD-Score:3.43|P-value:4.24E-4 ID:SGTC_1105|Compound:epirubicin|FD-Score:-4.05|P-value:3.87E-5 ID:SGTC_1388|Compound:3346-4487|FD-Score:3.20|P-value:9.39E-4 ID:SGTC_1402|Compound:3909-7673|FD-Score:3.52|P-value:3.11E-4 ID:SGTC_1428|Compound:4049-0204|FD-Score:3.52|P-value:3.18E-4 ID:SGTC_1457|Compound:k015-0032|FD-Score:-3.17|P-value:9.83E-4 ID:SGTC_1632|Compound:st005650|FD-Score:3.24|P-value:8.13E-4 ID:SGTC_1657|Compound:st012939|FD-Score:3.24|P-value:8.37E-4 ID:SGTC_1784|Compound:st039334|FD-Score:6.16|P-value:1.05E-9 ID:SGTC_2002|Compound:4023503|FD-Score:-5.48|P-value:4.53E-8 ID:SGTC_2012|Compound:4023501|FD-Score:-4.45|P-value:7.28E-6 ID:SGTC_2023|Compound:5144124|FD-Score:3.34|P-value:5.91E-4 ID:SGTC_2102|Compound:5377816|FD-Score:4.18|P-value:2.45E-5 ID:SGTC_2206|Compound:7254556|FD-Score:3.63|P-value:2.12E-4 ID:SGTC_2223|Compound:6903751|FD-Score:3.96|P-value:5.86E-5 ID:SGTC_2274|Compound:7947094|FD-Score:-3.95|P-value:5.96E-5 ID:SGTC_2347|Compound:9049783|FD-Score:-3.21|P-value:8.61E-4 ID:SGTC_2469|Compound:5655019|FD-Score:4.25|P-value:1.79E-5 ID:SGTC_2488|Compound:5268135|FD-Score:3.37|P-value:5.23E-4 ID:SGTC_2561|Compound:acetosyringone|FD-Score:3.24|P-value:8.23E-4 ID:SGTC_2564|Compound:betulinic acid|FD-Score:13.70|P-value:8.08E-41 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:4.82|P-value:1.43E-6 ID:SGTC_2578|Compound:7,8-dihydroxyflavone|FD-Score:-3.48|P-value:3.48E-4 ID:SGTC_2593|Compound:nobiletin|FD-Score:3.91|P-value:7.38E-5 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:4.44|P-value:8.07E-6 ID:SGTC_2701|Compound:st077767|FD-Score:-3.48|P-value:3.43E-4 ID:SGTC_2703|Compound:st077852|FD-Score:5.91|P-value:4.61E-9 ID:SGTC_2750|Compound:bromebric acid|FD-Score:3.71|P-value:1.55E-4 ID:SGTC_2762|Compound:leflunomide|FD-Score:3.84|P-value:9.51E-5 ID:SGTC_2788|Compound:5282734|FD-Score:3.58|P-value:2.57E-4 ID:SGTC_2989|Compound:9073649|FD-Score:-3.27|P-value:7.03E-4 ID:SGTC_3044|Compound:9093193|FD-Score:-3.39|P-value:4.69E-4 ID:SGTC_3050|Compound:9093513|FD-Score:3.41|P-value:4.56E-4 ID:SGTC_3054|Compound:9094397|FD-Score:4.42|P-value:8.59E-6 ID:SGTC_3098|Compound:9119039|FD-Score:3.35|P-value:5.62E-4 ID:SGTC_3147|Compound:9097027|FD-Score:3.65|P-value:1.92E-4 ID:SGTC_3148|Compound:9097893|FD-Score:3.48|P-value:3.63E-4 ID:SGTC_3282|Compound:9108498|FD-Score:-3.53|P-value:2.84E-4 ID:SGTC_3292|Compound:9120825|FD-Score:-3.37|P-value:5.03E-4

Top fitness defect scores for YBR070C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2564 betulinic acid 13.70 100.0 µM 8.08E-41 1 5.10E-43 7.63
2 SGTC_1784 st039334 6.16 36.3 µM 1.05E-9 2 3.60E-10 0.74
3 SGTC_2703 st077852 5.91 53.4 µM 4.61E-9 2 1.72E-9 1.37
4 SGTC_964 1287-0107 4.84 19.4 µM 1.25E-6 cell wall 11 6.38E-7 0.08
5 SGTC_2567 aureobasidin a 4.82 60.0 nM 1.43E-6 30 7.30E-7 0.03
6 SGTC_738 1315-0003 4.54 72.4 µM 5.17E-6 4 2.85E-6 0.24
7 SGTC_2599 2',3-dihydroxy-4,4',6'-trimethoxychalcone 4.44 100.0 µM 8.07E-6 6 4.55E-6 0.02
8 SGTC_3054 9094397 4.42 49.5 µM 8.59E-6 fatty acid desaturase (OLE1) 4 4.86E-6 0.08
9 SGTC_2469 5655019 4.25 90.8 µM 1.79E-5 2 1.05E-5 0.63
10 SGTC_2102 5377816 4.18 72.8 µM 2.45E-5 3 1.47E-5 0.34
11 SGTC_993 3909-8757 4.07 62.7 µM 3.89E-5 3 2.39E-5 0.05
12 SGTC_2223 6903751 3.96 107.6 µM 5.86E-5 superoxide 7 3.68E-5 0.17
13 SGTC_2593 nobiletin 3.91 86.2 µM 7.38E-5 11 4.69E-5 0.06
14 SGTC_2762 leflunomide 3.84 42.1 µM 9.51E-5 4 6.13E-5 0.00
15 SGTC_2750 bromebric acid 3.71 70.2 µM 1.55E-4 7 1.03E-4 0.06
16 SGTC_260 nsc-65238 3.70 4.0 µM 1.60E-4 endomembrane recycling 5 1.06E-4 0.01
17 SGTC_3147 9097027 3.65 49.5 µM 1.92E-4 12 1.29E-4 0.04
18 SGTC_2206 7254556 3.63 147.0 µM 2.12E-4 60S ribosome export 5 1.43E-4 0.13
19 SGTC_2788 5282734 3.58 71.4 µM 2.57E-4 15 1.75E-4 0.04
20 SGTC_1402 3909-7673 3.52 35.9 µM 3.11E-4 7 2.14E-4 0.24

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.186 1.81E-27 YCR088W ABP1 hom Actin-binding protein of the cortical actin cytoskeleton, important for activation of the Arp2/3 complex that plays a key role actin in cytoskeleton organization; phosphorylation within its PRR (Proline-Rich Region), mediated by Cdc28p and Pho85p, protects Abp1p from proteolysis mediated by its own PEST sequences
0.147 1.13E-17 YBL063W KIP1 hom Kinesin-related motor protein required for mitotic spindle assembly, chromosome segregation, and 2 micron plasmid partitioning; functionally redundant with Cin8p for chromosomal but not plasmid functions
0.140 3.47E-16 YKL004W AUR1 het Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance
0.133 9.11E-15 YGL217C_d YGL217C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF KIP3/YGL216W
0.131 2.85E-14 YLL019C KNS1 hom Protein kinase involved in negative regulation of PolIII transcription; effector kinase of the TOR signaling pathway and phosphorylates Rpc53p to regulate ribosome and tRNA biosynthesis; member of the LAMMER family of protein kinases, which are serine/threonine kinases also capable of phosphorylating tyrosine residues; capable of autophosphorylation
0.128 9.34E-14 YPR045C THP3 hom Protein that forms a complex with Csn12p that is recruited to transcribed genes and may have a role in transcription elongation; possibly involved in splicing based on pre-mRNA accumulation defect for many intron-containing genes
0.120 2.97E-12 YDR155C CPR1 hom Cytoplasmic peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds the drug cyclosporin A; protein abundance increases in response to DNA replication stress
0.119 4.82E-12 YDR367W KEI1 het Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene
0.114 4.18E-11 YDL114W_p YDL114W_p hom Putative short-chain dehydrogenase/reductase; YDL114W is not an essential gene
0.113 4.56E-11 YBL057C PTH2 hom One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth
0.112 8.36E-11 YBR211C AME1 het Essential kinetochore protein associated with microtubules and SPBs; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-U and fission yeast Mis17; relative distribution to the nucleus increases upon DNA replication stress
0.111 1.28E-10 YNL124W NAF1 het RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p
0.107 5.86E-10 YDR109C_p YDR109C_p hom Putative kinase
0.106 6.39E-10 YEL002C WBP1 het Beta subunit of the oligosaccharyl transferase (OST) glycoprotein complex; required for N-linked glycosylation of proteins in the endoplasmic reticulum
0.106 8.75E-10 YDR299W BFR2 het Essential protein that is a component of 90S preribosomes; may be involved in rRNA processing; multicopy suppressor of sensitivity to Brefeldin A; expression is induced during lag phase and also by cold shock