YBR077C / SLM4

Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; gene exhibits synthetic genetic interaction with MSS4

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR077C / SLM4

Click on Significant Values for Screen Details ID:SGTC_17|Compound:0960-0276|FD-Score:3.71|P-value:3.83E-4 ID:SGTC_56|Compound:0957-0365|FD-Score:9.09|P-value:5.44E-17 ID:SGTC_58|Compound:0957-0393|FD-Score:7.86|P-value:3.88E-13 ID:SGTC_71|Compound:0417-1750|FD-Score:5.44|P-value:3.65E-7 ID:SGTC_78|Compound:0336-0123|FD-Score:4.19|P-value:7.04E-5 ID:SGTC_138|Compound:0850-0259|FD-Score:9.22|P-value:1.93E-17 ID:SGTC_166|Compound:k072-0058|FD-Score:5.50|P-value:2.73E-7 ID:SGTC_210|Compound:3551-0445|FD-Score:3.52|P-value:7.02E-4 ID:SGTC_228|Compound:caffeine|FD-Score:8.59|P-value:2.30E-15 ID:SGTC_309|Compound:k408-0051|FD-Score:3.87|P-value:2.20E-4 ID:SGTC_311|Compound:1315-0136|FD-Score:4.54|P-value:1.85E-5 ID:SGTC_354|Compound:2783-3500|FD-Score:3.76|P-value:3.30E-4 ID:SGTC_356|Compound:1634-0012|FD-Score:5.73|P-value:9.28E-8 ID:SGTC_393|Compound:acivicin|FD-Score:-3.80|P-value:2.16E-4 ID:SGTC_451|Compound:chloral hydrate|FD-Score:4.51|P-value:2.11E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:5.96|P-value:2.82E-8 ID:SGTC_525|Compound:k781-2675|FD-Score:-3.41|P-value:7.65E-4 ID:SGTC_574|Compound:1315-0397|FD-Score:5.13|P-value:1.55E-6 ID:SGTC_586|Compound:0957-0265|FD-Score:4.48|P-value:2.37E-5 ID:SGTC_640|Compound:3381-0960|FD-Score:3.94|P-value:1.78E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:4.20|P-value:6.99E-5 ID:SGTC_736|Compound:0958-0254|FD-Score:3.93|P-value:1.84E-4 ID:SGTC_837|Compound:0091-0265|FD-Score:3.65|P-value:4.69E-4 ID:SGTC_985|Compound:1493-0307|FD-Score:3.46|P-value:8.61E-4 ID:SGTC_1146|Compound:3579-1023|FD-Score:3.98|P-value:1.55E-4 ID:SGTC_1203|Compound:3992-3522|FD-Score:-3.38|P-value:8.43E-4 ID:SGTC_1218|Compound:1348-1416|FD-Score:4.85|P-value:5.23E-6 ID:SGTC_1242|Compound:0402-0103|FD-Score:-3.72|P-value:2.77E-4 ID:SGTC_1259|Compound:0671-0132|FD-Score:3.57|P-value:6.13E-4 ID:SGTC_1313|Compound:1220-0047|FD-Score:3.90|P-value:2.00E-4 ID:SGTC_1513|Compound:0337-0559|FD-Score:-3.58|P-value:4.46E-4 ID:SGTC_1514|Compound:1189-1591|FD-Score:5.05|P-value:2.19E-6 ID:SGTC_1516|Compound:st013856|FD-Score:-4.28|P-value:3.66E-5 ID:SGTC_1518|Compound:st038567|FD-Score:3.96|P-value:1.63E-4 ID:SGTC_1526|Compound:st051518|FD-Score:3.58|P-value:5.81E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-4.94|P-value:2.48E-6 ID:SGTC_1734|Compound:st037168|FD-Score:3.82|P-value:2.67E-4 ID:SGTC_1749|Compound:st037932|FD-Score:-3.56|P-value:4.73E-4 ID:SGTC_1754|Compound:st042065|FD-Score:4.15|P-value:8.18E-5 ID:SGTC_1844|Compound:rapamycin|FD-Score:12.60|P-value:3.25E-31 ID:SGTC_1877|Compound:wortmannin|FD-Score:10.60|P-value:2.09E-22 ID:SGTC_1921|Compound:5740136|FD-Score:5.83|P-value:5.50E-8 ID:SGTC_1923|Compound:st058458|FD-Score:-3.53|P-value:5.28E-4 ID:SGTC_2018|Compound:5133068|FD-Score:3.43|P-value:9.56E-4 ID:SGTC_2026|Compound:5148700|FD-Score:4.04|P-value:1.23E-4 ID:SGTC_2127|Compound:5253358|FD-Score:4.74|P-value:8.34E-6 ID:SGTC_2177|Compound:5811945|FD-Score:8.83|P-value:3.76E-16 ID:SGTC_2178|Compound:5812956|FD-Score:4.11|P-value:9.49E-5 ID:SGTC_2340|Compound:7987427|FD-Score:-4.11|P-value:7.02E-5 ID:SGTC_2349|Compound:9023730|FD-Score:5.08|P-value:1.94E-6 ID:SGTC_2408|Compound:sulbentine|FD-Score:5.82|P-value:5.95E-8 ID:SGTC_2422|Compound:chlorphenesin carbamate|FD-Score:3.66|P-value:4.57E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:7.82|P-value:4.76E-13 ID:SGTC_2620|Compound:irigenin|FD-Score:4.12|P-value:9.37E-5 ID:SGTC_2651|Compound:1327-0036|FD-Score:6.20|P-value:7.98E-9 ID:SGTC_2679|Compound:rapamycin|FD-Score:17.00|P-value:8.19E-55 ID:SGTC_2705|Compound:st078069|FD-Score:3.69|P-value:4.14E-4 ID:SGTC_2766|Compound:oltipraz|FD-Score:4.78|P-value:7.11E-6 ID:SGTC_2912|Compound:7964298|FD-Score:5.85|P-value:5.07E-8 ID:SGTC_3013|Compound:9080133|FD-Score:3.47|P-value:8.26E-4 ID:SGTC_3037|Compound:9091026|FD-Score:4.60|P-value:1.46E-5 ID:SGTC_3302|Compound:9125395|FD-Score:3.66|P-value:4.48E-4 ID:SGTC_3350|Compound:9150499|FD-Score:7.41|P-value:7.21E-12 ID:SGTC_3351|Compound:9150312|FD-Score:6.53|P-value:1.35E-9 ID:SGTC_17|Compound:0960-0276|FD-Score:3.71|P-value:3.83E-4 ID:SGTC_56|Compound:0957-0365|FD-Score:9.09|P-value:5.44E-17 ID:SGTC_58|Compound:0957-0393|FD-Score:7.86|P-value:3.88E-13 ID:SGTC_71|Compound:0417-1750|FD-Score:5.44|P-value:3.65E-7 ID:SGTC_78|Compound:0336-0123|FD-Score:4.19|P-value:7.04E-5 ID:SGTC_138|Compound:0850-0259|FD-Score:9.22|P-value:1.93E-17 ID:SGTC_166|Compound:k072-0058|FD-Score:5.50|P-value:2.73E-7 ID:SGTC_210|Compound:3551-0445|FD-Score:3.52|P-value:7.02E-4 ID:SGTC_228|Compound:caffeine|FD-Score:8.59|P-value:2.30E-15 ID:SGTC_309|Compound:k408-0051|FD-Score:3.87|P-value:2.20E-4 ID:SGTC_311|Compound:1315-0136|FD-Score:4.54|P-value:1.85E-5 ID:SGTC_354|Compound:2783-3500|FD-Score:3.76|P-value:3.30E-4 ID:SGTC_356|Compound:1634-0012|FD-Score:5.73|P-value:9.28E-8 ID:SGTC_393|Compound:acivicin|FD-Score:-3.80|P-value:2.16E-4 ID:SGTC_451|Compound:chloral hydrate|FD-Score:4.51|P-value:2.11E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:5.96|P-value:2.82E-8 ID:SGTC_525|Compound:k781-2675|FD-Score:-3.41|P-value:7.65E-4 ID:SGTC_574|Compound:1315-0397|FD-Score:5.13|P-value:1.55E-6 ID:SGTC_586|Compound:0957-0265|FD-Score:4.48|P-value:2.37E-5 ID:SGTC_640|Compound:3381-0960|FD-Score:3.94|P-value:1.78E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:4.20|P-value:6.99E-5 ID:SGTC_736|Compound:0958-0254|FD-Score:3.93|P-value:1.84E-4 ID:SGTC_837|Compound:0091-0265|FD-Score:3.65|P-value:4.69E-4 ID:SGTC_985|Compound:1493-0307|FD-Score:3.46|P-value:8.61E-4 ID:SGTC_1146|Compound:3579-1023|FD-Score:3.98|P-value:1.55E-4 ID:SGTC_1203|Compound:3992-3522|FD-Score:-3.38|P-value:8.43E-4 ID:SGTC_1218|Compound:1348-1416|FD-Score:4.85|P-value:5.23E-6 ID:SGTC_1242|Compound:0402-0103|FD-Score:-3.72|P-value:2.77E-4 ID:SGTC_1259|Compound:0671-0132|FD-Score:3.57|P-value:6.13E-4 ID:SGTC_1313|Compound:1220-0047|FD-Score:3.90|P-value:2.00E-4 ID:SGTC_1513|Compound:0337-0559|FD-Score:-3.58|P-value:4.46E-4 ID:SGTC_1514|Compound:1189-1591|FD-Score:5.05|P-value:2.19E-6 ID:SGTC_1516|Compound:st013856|FD-Score:-4.28|P-value:3.66E-5 ID:SGTC_1518|Compound:st038567|FD-Score:3.96|P-value:1.63E-4 ID:SGTC_1526|Compound:st051518|FD-Score:3.58|P-value:5.81E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-4.94|P-value:2.48E-6 ID:SGTC_1734|Compound:st037168|FD-Score:3.82|P-value:2.67E-4 ID:SGTC_1749|Compound:st037932|FD-Score:-3.56|P-value:4.73E-4 ID:SGTC_1754|Compound:st042065|FD-Score:4.15|P-value:8.18E-5 ID:SGTC_1844|Compound:rapamycin|FD-Score:12.60|P-value:3.25E-31 ID:SGTC_1877|Compound:wortmannin|FD-Score:10.60|P-value:2.09E-22 ID:SGTC_1921|Compound:5740136|FD-Score:5.83|P-value:5.50E-8 ID:SGTC_1923|Compound:st058458|FD-Score:-3.53|P-value:5.28E-4 ID:SGTC_2018|Compound:5133068|FD-Score:3.43|P-value:9.56E-4 ID:SGTC_2026|Compound:5148700|FD-Score:4.04|P-value:1.23E-4 ID:SGTC_2127|Compound:5253358|FD-Score:4.74|P-value:8.34E-6 ID:SGTC_2177|Compound:5811945|FD-Score:8.83|P-value:3.76E-16 ID:SGTC_2178|Compound:5812956|FD-Score:4.11|P-value:9.49E-5 ID:SGTC_2340|Compound:7987427|FD-Score:-4.11|P-value:7.02E-5 ID:SGTC_2349|Compound:9023730|FD-Score:5.08|P-value:1.94E-6 ID:SGTC_2408|Compound:sulbentine|FD-Score:5.82|P-value:5.95E-8 ID:SGTC_2422|Compound:chlorphenesin carbamate|FD-Score:3.66|P-value:4.57E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:7.82|P-value:4.76E-13 ID:SGTC_2620|Compound:irigenin|FD-Score:4.12|P-value:9.37E-5 ID:SGTC_2651|Compound:1327-0036|FD-Score:6.20|P-value:7.98E-9 ID:SGTC_2679|Compound:rapamycin|FD-Score:17.00|P-value:8.19E-55 ID:SGTC_2705|Compound:st078069|FD-Score:3.69|P-value:4.14E-4 ID:SGTC_2766|Compound:oltipraz|FD-Score:4.78|P-value:7.11E-6 ID:SGTC_2912|Compound:7964298|FD-Score:5.85|P-value:5.07E-8 ID:SGTC_3013|Compound:9080133|FD-Score:3.47|P-value:8.26E-4 ID:SGTC_3037|Compound:9091026|FD-Score:4.60|P-value:1.46E-5 ID:SGTC_3302|Compound:9125395|FD-Score:3.66|P-value:4.48E-4 ID:SGTC_3350|Compound:9150499|FD-Score:7.41|P-value:7.21E-12 ID:SGTC_3351|Compound:9150312|FD-Score:6.53|P-value:1.35E-9

Top fitness defect scores for YBR077C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2679 rapamycin 17.00 1.0 nM 8.19E-55 4 4.42E-65
2 SGTC_1844 rapamycin 12.60 4.0 nM 3.25E-31 18 5.74E-37
3 SGTC_1877 wortmannin 10.60 25.0 µM 2.09E-22 9 1.80E-26
4 SGTC_138 0850-0259 9.22 40.9 µM 1.93E-17 cell wall 52 1.49E-20
5 SGTC_56 0957-0365 9.09 83.2 µM 5.44E-17 Golgi 8 5.10E-20
6 SGTC_2177 5811945 8.83 192.0 µM 3.76E-16 3 5.12E-19
7 SGTC_228 caffeine 8.59 993.1 µM 2.30E-15 2 4.43E-18
8 SGTC_58 0957-0393 7.86 9.0 µM 3.88E-13 cell wall 129 2.00E-15
9 SGTC_2619 obliquin 7.82 87.9 µM 4.76E-13 cell wall 44 2.55E-15
10 SGTC_3350 9150499 7.41 3.6 µM 7.21E-12 cell wall 9 6.49E-14
11 SGTC_3351 9150312 6.53 21.6 µM 1.35E-9 cell wall 4 3.30E-11
12 SGTC_2651 1327-0036 6.20 5.0 µM 7.98E-9 67 2.75E-10
13 SGTC_453 enantio-paf c16 5.96 10.0 µM 2.82E-8 52 1.24E-9
14 SGTC_2912 7964298 5.85 10.9 µM 5.07E-8 cell wall 47 2.49E-9
15 SGTC_1921 5740136 5.83 50.0 µM 5.50E-8 TRP & mitochondrial translation 16 2.74E-9
16 SGTC_2408 sulbentine 5.82 18.4 µM 5.95E-8 24 3.02E-9
17 SGTC_356 1634-0012 5.73 32.5 µM 9.28E-8 7 5.12E-9
18 SGTC_166 k072-0058 5.50 166.4 µM 2.73E-7 DNA intercalators 9 1.85E-8
19 SGTC_71 0417-1750 5.44 47.8 µM 3.65E-7 Golgi 20 2.61E-8
20 SGTC_574 1315-0397 5.13 40.0 µM 1.55E-6 10 1.46E-7

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.572 1.50E-289 YML121W GTR1 hom Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB
0.516 8.47E-227 YGR163W GTR2 hom Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD
0.461 1.67E-175 YKR007W MEH1 hom Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; loss results in a defect in vacuolar acidification
0.351 9.82E-98 YLL043W FPS1 hom Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress
0.341 1.32E-91 YJR066W TOR1 hom PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication
0.338 5.96E-90 YHR186C KOG1 het Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors
0.324 8.01E-83 YPL180W TCO89 hom Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p), a complex that regulates growth in response to nutrient availability; cooperates with Ssd1p in the maintenance of cellular integrity; deletion strains are hypersensitive to rapamycin
0.313 4.05E-77 YKL203C TOR2 het PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
0.281 1.44E-61 YLR114C AVL9 hom Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.261 3.61E-53 YCR075W-A_p YCR075W-A_p hom Putative protein of unknown function; identified by homology to Ashbya gossypii; YCR075W-A has a paralog, YNR034W-A, that arose from the whole genome duplication
0.253 7.59E-50 YLR331C_d JIP3_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
0.242 7.45E-46 YKL110C KTI12 hom Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p
0.241 1.75E-45 YLR384C IKI3 hom Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD)
0.237 5.10E-44 YHR187W IKI1 hom Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin
0.235 5.02E-43 YNL119W NCS2 hom Protein required for thiolation of the uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae