YBR080C / SEC18

ATPase required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, autophagy, and protein secretion; releases Sec17p from SNAP complexes; has similarity to mammalian NSF

Zygosity: Heterozygous strain
fixedexpanded
Profile for YBR080C / SEC18

Click on Significant Values for Screen Details ID:SGTC_67|Compound:0335-0847|FD-Score:3.77|P-value:1.29E-5 ID:SGTC_144|Compound:4161-2749|FD-Score:3.30|P-value:1.17E-4 ID:SGTC_145|Compound:3737-0113|FD-Score:2.92|P-value:5.57E-4 ID:SGTC_226|Compound:1,3-diallylurea|FD-Score:2.79|P-value:9.20E-4 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:3.73|P-value:1.57E-5 ID:SGTC_565|Compound:1418-0001|FD-Score:2.92|P-value:5.59E-4 ID:SGTC_634|Compound:1611-4317|FD-Score:3.06|P-value:3.14E-4 ID:SGTC_787|Compound:4152-0075|FD-Score:-2.85|P-value:8.03E-4 ID:SGTC_805|Compound:3852-0218|FD-Score:-4.00|P-value:4.73E-6 ID:SGTC_808|Compound:0711-0032|FD-Score:4.18|P-value:1.58E-6 ID:SGTC_1062|Compound:mesoridazine|FD-Score:-3.49|P-value:5.63E-5 ID:SGTC_1065|Compound:prochlorperazine maleate|FD-Score:-3.13|P-value:2.62E-4 ID:SGTC_1088|Compound:bifonazole|FD-Score:3.50|P-value:4.80E-5 ID:SGTC_1110|Compound:3-hydroxy-3'-methoxyflavone|FD-Score:-3.10|P-value:3.03E-4 ID:SGTC_1138|Compound:3761-0006|FD-Score:4.54|P-value:2.08E-7 ID:SGTC_1148|Compound:3909-7663|FD-Score:-3.09|P-value:3.16E-4 ID:SGTC_1153|Compound:4055-0057|FD-Score:-3.44|P-value:6.99E-5 ID:SGTC_1218|Compound:1348-1416|FD-Score:-3.11|P-value:2.91E-4 ID:SGTC_1246|Compound:0422-0085|FD-Score:3.17|P-value:2.00E-4 ID:SGTC_1335|Compound:1486-0900|FD-Score:-2.95|P-value:5.45E-4 ID:SGTC_1529|Compound:apigenin|FD-Score:3.45|P-value:5.97E-5 ID:SGTC_1563|Compound:conessine|FD-Score:-3.06|P-value:3.55E-4 ID:SGTC_1564|Compound:D-Arginine|FD-Score:4.72|P-value:7.40E-8 ID:SGTC_1567|Compound:st069303|FD-Score:6.54|P-value:1.62E-13 ID:SGTC_1570|Compound:verbenone|FD-Score:2.79|P-value:9.20E-4 ID:SGTC_1572|Compound:epiandrosterone|FD-Score:-3.08|P-value:3.24E-4 ID:SGTC_1601|Compound:4'-hydroxyflavanone|FD-Score:2.85|P-value:7.25E-4 ID:SGTC_1650|Compound:st011916|FD-Score:3.45|P-value:6.00E-5 ID:SGTC_1661|Compound:st013063|FD-Score:-3.11|P-value:2.90E-4 ID:SGTC_1667|Compound:st014849|FD-Score:-4.03|P-value:3.86E-6 ID:SGTC_1741|Compound:st038134|FD-Score:2.80|P-value:8.83E-4 ID:SGTC_1848|Compound:st056003|FD-Score:-3.10|P-value:2.99E-4 ID:SGTC_1934|Compound:st074720|FD-Score:-2.92|P-value:6.07E-4 ID:SGTC_1999|Compound:st074831|FD-Score:3.98|P-value:4.45E-6 ID:SGTC_2042|Compound:p-Dimethylaminoacetophenone|FD-Score:-2.89|P-value:6.83E-4 ID:SGTC_2071|Compound:5230951|FD-Score:-2.83|P-value:8.58E-4 ID:SGTC_2335|Compound:9018011|FD-Score:3.24|P-value:1.52E-4 ID:SGTC_2343|Compound:7990716|FD-Score:-4.92|P-value:2.36E-8 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:3.77|P-value:1.28E-5 ID:SGTC_2594|Compound:a-mangostin|FD-Score:3.07|P-value:3.06E-4 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:3.23|P-value:1.57E-4 ID:SGTC_2601|Compound:securinine|FD-Score:-4.34|P-value:7.43E-7 ID:SGTC_2785|Compound:7756463|FD-Score:2.97|P-value:4.52E-4 ID:SGTC_2940|Compound:9060638|FD-Score:-3.64|P-value:2.80E-5 ID:SGTC_2999|Compound:9069765|FD-Score:2.91|P-value:5.80E-4 ID:SGTC_3202|Compound:9113761|FD-Score:4.66|P-value:1.02E-7 ID:SGTC_3204|Compound:9114183|FD-Score:3.34|P-value:9.83E-5 ID:SGTC_3244|Compound:9134489|FD-Score:2.92|P-value:5.58E-4 ID:SGTC_3299|Compound:9121982|FD-Score:3.15|P-value:2.18E-4 ID:SGTC_3326|Compound:9140064|FD-Score:3.05|P-value:3.30E-4 ID:SGTC_67|Compound:0335-0847|FD-Score:3.77|P-value:1.29E-5 ID:SGTC_144|Compound:4161-2749|FD-Score:3.30|P-value:1.17E-4 ID:SGTC_145|Compound:3737-0113|FD-Score:2.92|P-value:5.57E-4 ID:SGTC_226|Compound:1,3-diallylurea|FD-Score:2.79|P-value:9.20E-4 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:3.73|P-value:1.57E-5 ID:SGTC_565|Compound:1418-0001|FD-Score:2.92|P-value:5.59E-4 ID:SGTC_634|Compound:1611-4317|FD-Score:3.06|P-value:3.14E-4 ID:SGTC_787|Compound:4152-0075|FD-Score:-2.85|P-value:8.03E-4 ID:SGTC_805|Compound:3852-0218|FD-Score:-4.00|P-value:4.73E-6 ID:SGTC_808|Compound:0711-0032|FD-Score:4.18|P-value:1.58E-6 ID:SGTC_1062|Compound:mesoridazine|FD-Score:-3.49|P-value:5.63E-5 ID:SGTC_1065|Compound:prochlorperazine maleate|FD-Score:-3.13|P-value:2.62E-4 ID:SGTC_1088|Compound:bifonazole|FD-Score:3.50|P-value:4.80E-5 ID:SGTC_1110|Compound:3-hydroxy-3'-methoxyflavone|FD-Score:-3.10|P-value:3.03E-4 ID:SGTC_1138|Compound:3761-0006|FD-Score:4.54|P-value:2.08E-7 ID:SGTC_1148|Compound:3909-7663|FD-Score:-3.09|P-value:3.16E-4 ID:SGTC_1153|Compound:4055-0057|FD-Score:-3.44|P-value:6.99E-5 ID:SGTC_1218|Compound:1348-1416|FD-Score:-3.11|P-value:2.91E-4 ID:SGTC_1246|Compound:0422-0085|FD-Score:3.17|P-value:2.00E-4 ID:SGTC_1335|Compound:1486-0900|FD-Score:-2.95|P-value:5.45E-4 ID:SGTC_1529|Compound:apigenin|FD-Score:3.45|P-value:5.97E-5 ID:SGTC_1563|Compound:conessine|FD-Score:-3.06|P-value:3.55E-4 ID:SGTC_1564|Compound:D-Arginine|FD-Score:4.72|P-value:7.40E-8 ID:SGTC_1567|Compound:st069303|FD-Score:6.54|P-value:1.62E-13 ID:SGTC_1570|Compound:verbenone|FD-Score:2.79|P-value:9.20E-4 ID:SGTC_1572|Compound:epiandrosterone|FD-Score:-3.08|P-value:3.24E-4 ID:SGTC_1601|Compound:4'-hydroxyflavanone|FD-Score:2.85|P-value:7.25E-4 ID:SGTC_1650|Compound:st011916|FD-Score:3.45|P-value:6.00E-5 ID:SGTC_1661|Compound:st013063|FD-Score:-3.11|P-value:2.90E-4 ID:SGTC_1667|Compound:st014849|FD-Score:-4.03|P-value:3.86E-6 ID:SGTC_1741|Compound:st038134|FD-Score:2.80|P-value:8.83E-4 ID:SGTC_1848|Compound:st056003|FD-Score:-3.10|P-value:2.99E-4 ID:SGTC_1934|Compound:st074720|FD-Score:-2.92|P-value:6.07E-4 ID:SGTC_1999|Compound:st074831|FD-Score:3.98|P-value:4.45E-6 ID:SGTC_2042|Compound:p-Dimethylaminoacetophenone|FD-Score:-2.89|P-value:6.83E-4 ID:SGTC_2071|Compound:5230951|FD-Score:-2.83|P-value:8.58E-4 ID:SGTC_2335|Compound:9018011|FD-Score:3.24|P-value:1.52E-4 ID:SGTC_2343|Compound:7990716|FD-Score:-4.92|P-value:2.36E-8 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:3.77|P-value:1.28E-5 ID:SGTC_2594|Compound:a-mangostin|FD-Score:3.07|P-value:3.06E-4 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:3.23|P-value:1.57E-4 ID:SGTC_2601|Compound:securinine|FD-Score:-4.34|P-value:7.43E-7 ID:SGTC_2785|Compound:7756463|FD-Score:2.97|P-value:4.52E-4 ID:SGTC_2940|Compound:9060638|FD-Score:-3.64|P-value:2.80E-5 ID:SGTC_2999|Compound:9069765|FD-Score:2.91|P-value:5.80E-4 ID:SGTC_3202|Compound:9113761|FD-Score:4.66|P-value:1.02E-7 ID:SGTC_3204|Compound:9114183|FD-Score:3.34|P-value:9.83E-5 ID:SGTC_3244|Compound:9134489|FD-Score:2.92|P-value:5.58E-4 ID:SGTC_3299|Compound:9121982|FD-Score:3.15|P-value:2.18E-4 ID:SGTC_3326|Compound:9140064|FD-Score:3.05|P-value:3.30E-4

Top fitness defect scores for YBR080C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1567 st069303 6.54 68.4 µM 1.62E-13 mitochondrial processes 3 3.07E-11 0.99
2 SGTC_1564 D-Arginine 4.72 115.0 µM 7.40E-8 4 1.21E-6 0.42
3 SGTC_3202 9113761 4.66 49.5 µM 1.02E-7 5 1.57E-6 0.15
4 SGTC_1138 3761-0006 4.54 1.0 µM 2.08E-7 1 2.80E-6 1.08
5 SGTC_808 0711-0032 4.18 135.0 µM 1.58E-6 8 1.45E-5 0.21
6 SGTC_1999 st074831 3.98 12.5 µM 4.45E-6 plasma membrane duress 10 3.39E-5 0.14
7 SGTC_2566 crystal violet carbinol 3.77 226.0 nM 1.28E-5 21 8.03E-5 0.00
8 SGTC_67 0335-0847 3.77 266.0 µM 1.29E-5 ubiquinone biosynthesis & proteasome 26 8.08E-5 0.24
9 SGTC_242 5hr heat shock (37°C) + 5-fluorouracil 3.73 4.6 µM 1.57E-5 heat shock/prefoldin 22 9.47E-5 0.02
10 SGTC_1088 bifonazole 3.50 369.0 nM 4.80E-5 4 2.35E-4 0.12
11 SGTC_1529 apigenin 3.45 74.0 µM 5.97E-5 8 2.81E-4 0.11
12 SGTC_1650 st011916 3.45 45.8 µM 6.00E-5 4 2.83E-4 0.11
13 SGTC_3204 9114183 3.34 49.5 µM 9.83E-5 13 4.23E-4 0.06
14 SGTC_144 4161-2749 3.30 112.4 µM 1.17E-4 DNA intercalators 24 4.89E-4 0.01
15 SGTC_2335 9018011 3.24 154.2 µM 1.52E-4 18 6.05E-4 0.08
16 SGTC_2599 2',3-dihydroxy-4,4',6'-trimethoxychalcone 3.23 100.0 µM 1.57E-4 14 6.19E-4 0.00
17 SGTC_1246 0422-0085 3.17 105.0 µM 2.00E-4 19 7.57E-4 0.13
18 SGTC_3299 9121982 3.15 43.1 µM 2.18E-4 11 8.10E-4 0.15
19 SGTC_2594 a-mangostin 3.07 100.0 µM 3.06E-4 19 0.00107 0.02
20 SGTC_634 1611-4317 3.06 11.2 µM 3.14E-4 ERG2 18 0.00109 0.16

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.151 1.49E-18 YPL011C TAF3 het TFIID subunit (47 kDa), involved in promoter binding and RNA polymerase II transcription initiation
0.120 3.38E-12 YOR291W YPK9 hom Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-type ATPase Spf1p; homolog of human ATP13A2 (PARK9), mutations in which are associated with Parkinson disease and Kufor-Rakeb syndrome
0.119 5.17E-12 YEL004W YEA4 hom Uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) transporter required for cell wall chitin synthesis; localized to the ER
0.113 5.39E-11 YJR041C URB2 het Nucleolar protein required for normal metabolism of the rRNA primary transcript, proposed to be involved in ribosome biogenesis
0.105 1.23E-9 YPL155C KIP2 hom Kinesin-related motor protein involved in mitotic spindle positioning, stabilizes microtubules by targeting Bik1p to the plus end; Kip2p levels are controlled during the cell cycle
0.103 2.48E-9 YKL015W PUT3 hom Transcriptional activator of proline utilization genes, constitutively binds PUT1 and PUT2 promoter sequences as a dimer and undergoes a conformational change to form the active state; differentially phosphorylated in the presence of different nitrogen sources; has a Zn(2)-Cys(6) binuclear cluster domain
0.099 9.89E-9 YBR169C SSE2 hom Member of the heat shock protein 70 (HSP70) family; may be involved in protein folding; localized to the cytoplasm; SSE2 has a paralog, SSE1, that arose from the whole genome duplication
0.096 2.17E-8 YDR454C GUK1 het Guanylate kinase, converts GMP to GDP; required for growth and mannose outer chain elongation of cell wall N-linked glycoproteins
0.094 4.26E-8 YPL160W CDC60 het Cytosolic leucyl tRNA synthetase, ligates leucine to the appropriate tRNA
0.094 4.44E-8 YMR110C HFD1 hom Hexadecenal dehydrogenase; involved in the conversion of sphingosine 1-phosphate breakdown product hexadecenal to hexadecenoic acid; located in the mitochondrial outer membrane and also in lipid particles; has similarity to ALDH3A2, a human fatty aldehyde dehydrogenase (FALDH) mutated in Sjogren-Larsson syndrome, a neurocutaneous disorder
0.093 6.48E-8 YOR230W WTM1 hom Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; required for nuclear localization of the ribonucleotide reductase small subunit Rnr2p and Rnr4p; contains WD repeats
0.092 8.21E-8 YMR092C AIP1 hom Actin cortical patch component; interacts with the actin depolymerizing factor cofilin; required to restrict cofilin localization to cortical patches; contains WD repeats; protein increases in abundance and relocalizes from cytoplasm to plasma membrane upon DNA replication stress
0.089 2.25E-7 YLR335W NUP2 hom Nucleoporin involved in nucleocytoplasmic transport, binds to either the nucleoplasmic or cytoplasmic faces of the nuclear pore complex depending on Ran-GTP levels; also has a role in chromatin organization
0.088 3.29E-7 YER014C-A BUD25 hom Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern
0.088 3.36E-7 YPR022C_p YPR022C_p hom Putative transcription factor, as suggested by computational analysis; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS