YBR082C / UBC4

Ubiquitin-conjugating enzyme (E2); mediates degradation of abnormal or excess proteins, including calmodulin and histone H3; interacts with many SCF ubiquitin protein ligases; component of the cellular stress response; UBC4 has a paralog, UBC5, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR082C / UBC4

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:5.19|P-value:4.91E-8 ID:SGTC_77|Compound:0828-0286|FD-Score:-3.40|P-value:3.67E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:6.18|P-value:1.16E-10 ID:SGTC_298|Compound:1391-0729|FD-Score:-3.78|P-value:8.30E-5 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.83|P-value:6.79E-5 ID:SGTC_312|Compound:4092-0653|FD-Score:-3.64|P-value:1.47E-4 ID:SGTC_314|Compound:1671-0008|FD-Score:-3.19|P-value:7.71E-4 ID:SGTC_320|Compound:4092-0846|FD-Score:-3.28|P-value:5.69E-4 ID:SGTC_359|Compound:0081-0058|FD-Score:-3.24|P-value:6.50E-4 ID:SGTC_377|Compound:3970-0964|FD-Score:3.53|P-value:1.31E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:-6.30|P-value:1.31E-10 ID:SGTC_433|Compound:pimozide|FD-Score:4.72|P-value:6.07E-7 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:3.23|P-value:4.16E-4 ID:SGTC_471|Compound:1-acyl-paf|FD-Score:3.21|P-value:4.44E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:3.44|P-value:1.87E-4 ID:SGTC_546|Compound:peri-Naphthalide|FD-Score:3.23|P-value:4.15E-4 ID:SGTC_558|Compound:k064-0027|FD-Score:3.58|P-value:1.08E-4 ID:SGTC_622|Compound:1348-1566|FD-Score:5.00|P-value:1.38E-7 ID:SGTC_690|Compound:glyoxal|FD-Score:4.31|P-value:4.43E-6 ID:SGTC_791|Compound:1348-1292|FD-Score:3.21|P-value:4.50E-4 ID:SGTC_895|Compound:0964-0006|FD-Score:4.59|P-value:1.13E-6 ID:SGTC_906|Compound:1165-0455|FD-Score:4.15|P-value:9.32E-6 ID:SGTC_911|Compound:3456-4043|FD-Score:3.60|P-value:1.01E-4 ID:SGTC_923|Compound:1900-2434|FD-Score:-3.85|P-value:6.33E-5 ID:SGTC_978|Compound:1416-0512|FD-Score:3.01|P-value:9.04E-4 ID:SGTC_1008|Compound:4106-0037|FD-Score:4.77|P-value:4.69E-7 ID:SGTC_1011|Compound:4112-3381|FD-Score:-5.21|P-value:9.03E-8 ID:SGTC_1026|Compound:k007-1105|FD-Score:-4.90|P-value:4.87E-7 ID:SGTC_1052|Compound:3253-3998|FD-Score:4.23|P-value:6.50E-6 ID:SGTC_1082|Compound:camptothecin|FD-Score:3.44|P-value:1.89E-4 ID:SGTC_1106|Compound:tegaserod maleate|FD-Score:3.14|P-value:5.66E-4 ID:SGTC_1108|Compound:betulinic acid|FD-Score:-6.98|P-value:1.17E-12 ID:SGTC_1111|Compound:tnp00062|FD-Score:-4.32|P-value:7.97E-6 ID:SGTC_1121|Compound:fusaric acid|FD-Score:-3.25|P-value:6.26E-4 ID:SGTC_1123|Compound:mangostin|FD-Score:-3.46|P-value:2.93E-4 ID:SGTC_1133|Compound:3473-0247|FD-Score:5.47|P-value:1.00E-8 ID:SGTC_1137|Compound:ixabepilone|FD-Score:6.42|P-value:2.42E-11 ID:SGTC_1374|Compound:2889-5571|FD-Score:3.51|P-value:1.41E-4 ID:SGTC_1583|Compound:clindamycin|FD-Score:3.56|P-value:1.18E-4 ID:SGTC_1584|Compound:6-fluoro-dl-tryptophan|FD-Score:3.41|P-value:2.09E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:-3.47|P-value:2.81E-4 ID:SGTC_1601|Compound:4'-hydroxyflavanone|FD-Score:-3.83|P-value:6.87E-5 ID:SGTC_1707|Compound:st032323|FD-Score:3.74|P-value:5.60E-5 ID:SGTC_1807|Compound:st050092|FD-Score:3.83|P-value:3.90E-5 ID:SGTC_1808|Compound:st050063|FD-Score:3.43|P-value:1.98E-4 ID:SGTC_2009|Compound:4012297|FD-Score:3.48|P-value:1.58E-4 ID:SGTC_2106|Compound:5357045|FD-Score:-3.18|P-value:8.08E-4 ID:SGTC_2124|Compound:5246320|FD-Score:3.56|P-value:1.16E-4 ID:SGTC_2248|Compound:tofa|FD-Score:3.07|P-value:7.43E-4 ID:SGTC_2329|Compound:6138490|FD-Score:3.57|P-value:1.14E-4 ID:SGTC_2333|Compound:9017922|FD-Score:-3.37|P-value:4.09E-4 ID:SGTC_2336|Compound:9001819|FD-Score:3.51|P-value:1.43E-4 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:6.83|P-value:1.36E-12 ID:SGTC_2582|Compound:agaric acid|FD-Score:15.70|P-value:5.14E-58 ID:SGTC_2607|Compound:solasodine|FD-Score:-5.80|P-value:3.10E-9 ID:SGTC_2608|Compound:thymoquinone|FD-Score:3.37|P-value:2.41E-4 ID:SGTC_2612|Compound:dictamnine|FD-Score:6.38|P-value:3.20E-11 ID:SGTC_2822|Compound:7994718|FD-Score:-4.12|P-value:1.95E-5 ID:SGTC_2870|Compound:9038528|FD-Score:3.58|P-value:1.09E-4 ID:SGTC_3269|Compound:9137823|FD-Score:3.19|P-value:4.70E-4 ID:SGTC_3271|Compound:9137798|FD-Score:3.40|P-value:2.20E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:5.19|P-value:4.91E-8 ID:SGTC_77|Compound:0828-0286|FD-Score:-3.40|P-value:3.67E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:6.18|P-value:1.16E-10 ID:SGTC_298|Compound:1391-0729|FD-Score:-3.78|P-value:8.30E-5 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.83|P-value:6.79E-5 ID:SGTC_312|Compound:4092-0653|FD-Score:-3.64|P-value:1.47E-4 ID:SGTC_314|Compound:1671-0008|FD-Score:-3.19|P-value:7.71E-4 ID:SGTC_320|Compound:4092-0846|FD-Score:-3.28|P-value:5.69E-4 ID:SGTC_359|Compound:0081-0058|FD-Score:-3.24|P-value:6.50E-4 ID:SGTC_377|Compound:3970-0964|FD-Score:3.53|P-value:1.31E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:-6.30|P-value:1.31E-10 ID:SGTC_433|Compound:pimozide|FD-Score:4.72|P-value:6.07E-7 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:3.23|P-value:4.16E-4 ID:SGTC_471|Compound:1-acyl-paf|FD-Score:3.21|P-value:4.44E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:3.44|P-value:1.87E-4 ID:SGTC_546|Compound:peri-Naphthalide|FD-Score:3.23|P-value:4.15E-4 ID:SGTC_558|Compound:k064-0027|FD-Score:3.58|P-value:1.08E-4 ID:SGTC_622|Compound:1348-1566|FD-Score:5.00|P-value:1.38E-7 ID:SGTC_690|Compound:glyoxal|FD-Score:4.31|P-value:4.43E-6 ID:SGTC_791|Compound:1348-1292|FD-Score:3.21|P-value:4.50E-4 ID:SGTC_895|Compound:0964-0006|FD-Score:4.59|P-value:1.13E-6 ID:SGTC_906|Compound:1165-0455|FD-Score:4.15|P-value:9.32E-6 ID:SGTC_911|Compound:3456-4043|FD-Score:3.60|P-value:1.01E-4 ID:SGTC_923|Compound:1900-2434|FD-Score:-3.85|P-value:6.33E-5 ID:SGTC_978|Compound:1416-0512|FD-Score:3.01|P-value:9.04E-4 ID:SGTC_1008|Compound:4106-0037|FD-Score:4.77|P-value:4.69E-7 ID:SGTC_1011|Compound:4112-3381|FD-Score:-5.21|P-value:9.03E-8 ID:SGTC_1026|Compound:k007-1105|FD-Score:-4.90|P-value:4.87E-7 ID:SGTC_1052|Compound:3253-3998|FD-Score:4.23|P-value:6.50E-6 ID:SGTC_1082|Compound:camptothecin|FD-Score:3.44|P-value:1.89E-4 ID:SGTC_1106|Compound:tegaserod maleate|FD-Score:3.14|P-value:5.66E-4 ID:SGTC_1108|Compound:betulinic acid|FD-Score:-6.98|P-value:1.17E-12 ID:SGTC_1111|Compound:tnp00062|FD-Score:-4.32|P-value:7.97E-6 ID:SGTC_1121|Compound:fusaric acid|FD-Score:-3.25|P-value:6.26E-4 ID:SGTC_1123|Compound:mangostin|FD-Score:-3.46|P-value:2.93E-4 ID:SGTC_1133|Compound:3473-0247|FD-Score:5.47|P-value:1.00E-8 ID:SGTC_1137|Compound:ixabepilone|FD-Score:6.42|P-value:2.42E-11 ID:SGTC_1374|Compound:2889-5571|FD-Score:3.51|P-value:1.41E-4 ID:SGTC_1583|Compound:clindamycin|FD-Score:3.56|P-value:1.18E-4 ID:SGTC_1584|Compound:6-fluoro-dl-tryptophan|FD-Score:3.41|P-value:2.09E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:-3.47|P-value:2.81E-4 ID:SGTC_1601|Compound:4'-hydroxyflavanone|FD-Score:-3.83|P-value:6.87E-5 ID:SGTC_1707|Compound:st032323|FD-Score:3.74|P-value:5.60E-5 ID:SGTC_1807|Compound:st050092|FD-Score:3.83|P-value:3.90E-5 ID:SGTC_1808|Compound:st050063|FD-Score:3.43|P-value:1.98E-4 ID:SGTC_2009|Compound:4012297|FD-Score:3.48|P-value:1.58E-4 ID:SGTC_2106|Compound:5357045|FD-Score:-3.18|P-value:8.08E-4 ID:SGTC_2124|Compound:5246320|FD-Score:3.56|P-value:1.16E-4 ID:SGTC_2248|Compound:tofa|FD-Score:3.07|P-value:7.43E-4 ID:SGTC_2329|Compound:6138490|FD-Score:3.57|P-value:1.14E-4 ID:SGTC_2333|Compound:9017922|FD-Score:-3.37|P-value:4.09E-4 ID:SGTC_2336|Compound:9001819|FD-Score:3.51|P-value:1.43E-4 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:6.83|P-value:1.36E-12 ID:SGTC_2582|Compound:agaric acid|FD-Score:15.70|P-value:5.14E-58 ID:SGTC_2607|Compound:solasodine|FD-Score:-5.80|P-value:3.10E-9 ID:SGTC_2608|Compound:thymoquinone|FD-Score:3.37|P-value:2.41E-4 ID:SGTC_2612|Compound:dictamnine|FD-Score:6.38|P-value:3.20E-11 ID:SGTC_2822|Compound:7994718|FD-Score:-4.12|P-value:1.95E-5 ID:SGTC_2870|Compound:9038528|FD-Score:3.58|P-value:1.09E-4 ID:SGTC_3269|Compound:9137823|FD-Score:3.19|P-value:4.70E-4 ID:SGTC_3271|Compound:9137798|FD-Score:3.40|P-value:2.20E-4

Top fitness defect scores for YBR082C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2582 agaric acid 15.70 100.0 µM 5.14E-58 redox potentiating 11 5.32E-56
2 SGTC_2502 avocadyne acetate 6.83 18.6 µM 1.36E-12 4 4.36E-12
3 SGTC_1137 ixabepilone 6.42 100.0 µM 2.42E-11 5 6.97E-11
4 SGTC_2612 dictamnine 6.38 15.5 µM 3.20E-11 RNA pol III & RNase P/MRP 10 9.12E-11
5 SGTC_268 camptothecin 6.18 39.6 µM 1.16E-10 DNA damage response 26 3.16E-10
6 SGTC_1133 3473-0247 5.47 50.0 µM 1.00E-8 RSC complex & mRNA processing 14 2.31E-8
7 SGTC_58 0957-0393 5.19 9.0 µM 4.91E-8 cell wall 264 1.06E-7
8 SGTC_622 1348-1566 5.00 26.3 µM 1.38E-7 7 2.86E-7
9 SGTC_1008 4106-0037 4.77 6.0 µM 4.69E-7 PDR1 8 9.29E-7
10 SGTC_433 pimozide 4.72 108.0 µM 6.07E-7 8 1.19E-6
11 SGTC_895 0964-0006 4.59 13.7 µM 1.13E-6 4 2.17E-6
12 SGTC_690 glyoxal 4.31 20.3 µM 4.43E-6 68 8.02E-6
13 SGTC_1052 3253-3998 4.23 198.0 µM 6.50E-6 10 1.16E-5
14 SGTC_906 1165-0455 4.15 526.0 µM 9.32E-6 9 1.64E-5
15 SGTC_1807 st050092 3.83 58.9 µM 3.90E-5 60S ribosome export 13 6.46E-5
16 SGTC_1707 st032323 3.74 6.3 µM 5.60E-5 DNA damage response 66 9.15E-5
17 SGTC_911 3456-4043 3.60 236.0 µM 1.01E-4 mitochondrial processes 37 1.61E-4
18 SGTC_558 k064-0027 3.58 21.3 µM 1.08E-4 DNA damage response 36 1.72E-4
19 SGTC_2870 9038528 3.58 13.0 µM 1.09E-4 26 1.73E-4
20 SGTC_2329 6138490 3.57 200.0 µM 1.14E-4 44 1.80E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.335 4.79E-89 YML103C NUP188 hom Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC organization and nucleocytoplasmic transport; homologous to human NUP188
0.322 7.14E-82 YNL106C INP52 hom Polyphosphatidylinositol phosphatase, dephosphorylates a number of phosphatidylinositols (PIs) to PI; involved in endocytosis; hyperosmotic stress causes translocation to actin patches; synaptojanin-like protein with a Sac1 domain
0.258 5.75E-52 YEL007W MIT1 hom Transcriptional regulator of pseudohyphal growth; protein with sequence similarity to S. pombe gti1+ (gluconate transport inducer 1) and C. albicans Wor1
0.245 6.05E-47 YOR049C RSB1 hom Suppressor of sphingoid long chain base (LCB) sensitivity of an LCB-lyase mutation; putative integral membrane transporter or flippase that may transport LCBs from the cytoplasmic side toward the extracytoplasmic side of the membrane
0.238 2.87E-44 YGL178W MPT5 hom mRNA-binding protein of the PUF family; binds to the 3' UTR of specific mRNAs, including those involved in mating type switching, cell wall integrity, chronological lifespan, chromatin modification, and spindle pole body architecture; recruits the CCR4-NOT deadenylase complex to mRNAs along with Dhh1p and Dcp1p to promote deadenylation, decapping, and decay; also interacts with the Caf20p translational initiation repressor, affecting its mRNA target specificity
0.236 1.29E-43 YNL215W IES2 hom Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress
0.235 3.30E-43 YML052W SUR7 hom Plasma membrane protein that localizes to furrow-like invaginations (MCC patches); component of eisosomes; associated with endocytosis, along with Pil1p and Lsp1p; sporulation and plasma membrane sphingolipid content are altered in mutants
0.234 6.70E-43 YNL027W CRZ1 hom Transcription factor, activates transcription of stress response genes; nuclear localization is positively regulated by calcineurin-mediated dephosphorylation; rapidly localizes to the nucleus under blue light stress
0.232 2.93E-42 YNL105W_d RRT16_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene INP52; identified in a screen for mutants with decreased levels of rDNA transcription
0.230 1.13E-41 YMR109W MYO5 hom One of two type I myosins; contains proline-rich tail homology 2 (TH2) and SH3 domains; MYO5 deletion has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization
0.223 3.51E-39 YOR050C_d YOR050C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutation is viable
0.222 7.18E-39 YOL013C HRD1 hom Ubiquitin-protein ligase; required for endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger; likely plays a general role in targeting proteins that persistently associate with and potentially obstruct the ER-localized translocon
0.215 1.77E-36 YML102W CAC2 hom Subunit of chromatin assembly factor I (CAF-1), with Rlf2p and Msi1p; chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription
0.215 2.69E-36 YLR206W ENT2 hom Epsin-like protein required for endocytosis and actin patch assembly and functionally redundant with Ent1p; contains clathrin-binding motif at C-terminus
0.214 3.17E-36 YIL129C TAO3 het Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with protein kinase Cbk1p and also with Kic1p