YBR086C / IST2

Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localizes to the mother cell in small-budded cells and to the bud in medium- and large-budded cells; mRNA is transported to the bud tip by an actomyosin-driven process

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR086C / IST2

Click on Significant Values for Screen Details ID:SGTC_36|Compound:0335-0881|FD-Score:-3.70|P-value:1.59E-4 ID:SGTC_42|Compound:0133-0086|FD-Score:-3.98|P-value:5.44E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:-4.48|P-value:6.51E-6 ID:SGTC_64|Compound:000l-0018|FD-Score:-3.96|P-value:5.74E-5 ID:SGTC_363|Compound:0455-0021|FD-Score:4.42|P-value:1.04E-5 ID:SGTC_365|Compound:0121-0052|FD-Score:-3.37|P-value:5.13E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:-3.70|P-value:1.56E-4 ID:SGTC_443|Compound:clozapine|FD-Score:-4.61|P-value:3.64E-6 ID:SGTC_500|Compound:h-89|FD-Score:-3.87|P-value:8.30E-5 ID:SGTC_562|Compound:3772-2733|FD-Score:3.66|P-value:2.16E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:3.42|P-value:5.10E-4 ID:SGTC_724|Compound:3910-0327|FD-Score:3.44|P-value:4.69E-4 ID:SGTC_922|Compound:1866-0035|FD-Score:8.03|P-value:4.63E-15 ID:SGTC_930|Compound:2810-3379|FD-Score:4.74|P-value:2.53E-6 ID:SGTC_959|Compound:1222-0044|FD-Score:-3.75|P-value:1.29E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:-3.24|P-value:7.99E-4 ID:SGTC_1087|Compound:pterostilbene|FD-Score:3.48|P-value:4.08E-4 ID:SGTC_1687|Compound:st020778|FD-Score:6.15|P-value:1.53E-9 ID:SGTC_1826|Compound:st055655|FD-Score:3.65|P-value:2.19E-4 ID:SGTC_1919|Compound:st067113|FD-Score:3.68|P-value:1.98E-4 ID:SGTC_1977|Compound:st070720|FD-Score:-3.28|P-value:6.92E-4 ID:SGTC_2018|Compound:5133068|FD-Score:3.61|P-value:2.58E-4 ID:SGTC_2051|Compound:5263410|FD-Score:3.33|P-value:6.86E-4 ID:SGTC_2162|Compound:5663267|FD-Score:4.53|P-value:6.38E-6 ID:SGTC_2321|Compound:7390090|FD-Score:3.30|P-value:7.48E-4 ID:SGTC_2529|Compound:gangaleoidin|FD-Score:5.23|P-value:2.35E-7 ID:SGTC_2533|Compound:agelasine|FD-Score:-3.18|P-value:9.58E-4 ID:SGTC_2534|Compound:helenine|FD-Score:3.38|P-value:5.83E-4 ID:SGTC_2788|Compound:5282734|FD-Score:-3.65|P-value:1.89E-4 ID:SGTC_3054|Compound:9094397|FD-Score:3.36|P-value:6.08E-4 ID:SGTC_3061|Compound:glucosamine|FD-Score:-3.50|P-value:3.20E-4 ID:SGTC_3153|Compound:9100258|FD-Score:-3.80|P-value:1.08E-4 ID:SGTC_3192|Compound:9110362|FD-Score:4.11|P-value:3.83E-5 ID:SGTC_3280|Compound:9140961|FD-Score:3.25|P-value:8.79E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:-3.70|P-value:1.59E-4 ID:SGTC_42|Compound:0133-0086|FD-Score:-3.98|P-value:5.44E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:-4.48|P-value:6.51E-6 ID:SGTC_64|Compound:000l-0018|FD-Score:-3.96|P-value:5.74E-5 ID:SGTC_363|Compound:0455-0021|FD-Score:4.42|P-value:1.04E-5 ID:SGTC_365|Compound:0121-0052|FD-Score:-3.37|P-value:5.13E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:-3.70|P-value:1.56E-4 ID:SGTC_443|Compound:clozapine|FD-Score:-4.61|P-value:3.64E-6 ID:SGTC_500|Compound:h-89|FD-Score:-3.87|P-value:8.30E-5 ID:SGTC_562|Compound:3772-2733|FD-Score:3.66|P-value:2.16E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:3.42|P-value:5.10E-4 ID:SGTC_724|Compound:3910-0327|FD-Score:3.44|P-value:4.69E-4 ID:SGTC_922|Compound:1866-0035|FD-Score:8.03|P-value:4.63E-15 ID:SGTC_930|Compound:2810-3379|FD-Score:4.74|P-value:2.53E-6 ID:SGTC_959|Compound:1222-0044|FD-Score:-3.75|P-value:1.29E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:-3.24|P-value:7.99E-4 ID:SGTC_1087|Compound:pterostilbene|FD-Score:3.48|P-value:4.08E-4 ID:SGTC_1687|Compound:st020778|FD-Score:6.15|P-value:1.53E-9 ID:SGTC_1826|Compound:st055655|FD-Score:3.65|P-value:2.19E-4 ID:SGTC_1919|Compound:st067113|FD-Score:3.68|P-value:1.98E-4 ID:SGTC_1977|Compound:st070720|FD-Score:-3.28|P-value:6.92E-4 ID:SGTC_2018|Compound:5133068|FD-Score:3.61|P-value:2.58E-4 ID:SGTC_2051|Compound:5263410|FD-Score:3.33|P-value:6.86E-4 ID:SGTC_2162|Compound:5663267|FD-Score:4.53|P-value:6.38E-6 ID:SGTC_2321|Compound:7390090|FD-Score:3.30|P-value:7.48E-4 ID:SGTC_2529|Compound:gangaleoidin|FD-Score:5.23|P-value:2.35E-7 ID:SGTC_2533|Compound:agelasine|FD-Score:-3.18|P-value:9.58E-4 ID:SGTC_2534|Compound:helenine|FD-Score:3.38|P-value:5.83E-4 ID:SGTC_2788|Compound:5282734|FD-Score:-3.65|P-value:1.89E-4 ID:SGTC_3054|Compound:9094397|FD-Score:3.36|P-value:6.08E-4 ID:SGTC_3061|Compound:glucosamine|FD-Score:-3.50|P-value:3.20E-4 ID:SGTC_3153|Compound:9100258|FD-Score:-3.80|P-value:1.08E-4 ID:SGTC_3192|Compound:9110362|FD-Score:4.11|P-value:3.83E-5 ID:SGTC_3280|Compound:9140961|FD-Score:3.25|P-value:8.79E-4

Top fitness defect scores for YBR086C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_922 1866-0035 8.03 480.0 µM 4.63E-15 excess fatty acid 9 4.97E-16
2 SGTC_1687 st020778 6.15 55.4 µM 1.53E-9 106 3.96E-10
3 SGTC_2529 gangaleoidin 5.23 93.6 µM 2.35E-7 6 8.59E-8
4 SGTC_930 2810-3379 4.74 265.0 µM 2.53E-6 5 1.09E-6
5 SGTC_2162 5663267 4.53 200.0 µM 6.38E-6 8 2.93E-6
6 SGTC_363 0455-0021 4.42 3.1 µM 1.04E-5 azole & statin 49 4.96E-6
7 SGTC_3192 9110362 4.11 49.5 µM 3.83E-5 mitochondrial processes 10 1.99E-5
8 SGTC_1919 st067113 3.68 52.3 µM 1.98E-4 TSC3-RPN4 40 1.16E-4
9 SGTC_562 3772-2733 3.66 112.0 µM 2.16E-4 ERG2 92 1.27E-4
10 SGTC_1826 st055655 3.65 41.6 µM 2.19E-4 ERG2 50 1.29E-4
11 SGTC_2018 5133068 3.61 143.0 µM 2.58E-4 31 1.53E-4
12 SGTC_1087 pterostilbene 3.48 9.1 µM 4.08E-4 46 2.50E-4
13 SGTC_724 3910-0327 3.44 137.0 µM 4.69E-4 ERG2 36 2.90E-4
14 SGTC_568 1193-0097 3.42 7.5 µM 5.10E-4 ERAD & cell cycle 183 3.18E-4
15 SGTC_2534 helenine 3.38 20.1 µM 5.83E-4 RSC complex & mRNA processing 23 3.66E-4
16 SGTC_3054 9094397 3.36 49.5 µM 6.08E-4 fatty acid desaturase (OLE1) 53 3.83E-4
17 SGTC_2051 5263410 3.33 176.0 µM 6.86E-4 35 4.36E-4
18 SGTC_2321 7390090 3.30 138.8 µM 7.48E-4 56 4.78E-4
19 SGTC_3280 9140961 3.25 49.5 µM 8.79E-4 60S ribosome export 36 5.68E-4
20 SGTC_626 0214-0009 3.21 33.0 µM 0.00100 copper-dependent oxidative stress 47 6.54E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.140 3.14E-16 YPL120W VPS30 hom Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1
0.138 1.04E-15 YJL024C APS3 hom Small subunit of the clathrin-associated adaptor complex AP-3; involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; protein abundance increases in response to DNA replication stress
0.131 2.65E-14 YOR007C SGT2 hom Glutamine-rich cytoplasmic cochaperone; serves as a scaffold bringing together Get4, Get5p, and other TRC complex members that are required to mediate posttranslational insertion of tail-anchored proteins into the ER membrane; interacts with the prion domain of Sup35p; amyloid sensor; plays a role in targeting chaperones to prion aggregates; has similarity to human cochaperone SGT; forms cytoplasmic foci upon DNA replication stress
0.122 1.37E-12 YLR087C CSF1 hom Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.122 1.44E-12 YOR024W_d YOR024W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.116 1.49E-11 YJR033C RAV1 hom Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate
0.116 1.73E-11 YGR261C APL6 hom Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools
0.112 7.50E-11 YDL100C GET3 hom Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress
0.108 3.36E-10 YNL190W_p YNL190W_p hom Hydrophilin essential in dessication-rehydration process; cell wall protein; contains a putative GPI-attachment site
0.107 4.71E-10 YEL062W NPR2 hom Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; Iml1p interacts primarily with phosphorylated Npr2p; homolog of human NPRL2; target of Grr1p; required for growth on urea and proline
0.107 5.25E-10 YLL011W SOF1 het Essential protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p
0.105 1.02E-9 YBR288C APM3 hom Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway
0.102 3.45E-9 YOR215C AIM41 hom Putative protein of unknown function; the authentic protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss
0.101 4.13E-9 YHL023C NPR3 hom Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; required for Npr2p phosphorylation and Iml1p-Npr2p interaction; null mutant shows delayed meiotic DNA replication and double-strand break repair
0.098 1.16E-8 YHL002W HSE1 hom Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes