YBR087W / RFC5

Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon

Zygosity: Heterozygous strain
fixedexpanded
Profile for YBR087W / RFC5

Click on Significant Values for Screen Details ID:SGTC_10|Compound:0279-0212|FD-Score:3.18|P-value:2.52E-4 ID:SGTC_69|Compound:0096-0274|FD-Score:6.47|P-value:5.69E-13 ID:SGTC_105|Compound:0111-0055|FD-Score:2.96|P-value:6.06E-4 ID:SGTC_136|Compound:0097-0015|FD-Score:-2.82|P-value:8.68E-4 ID:SGTC_342|Compound:k048-0088|FD-Score:3.28|P-value:1.66E-4 ID:SGTC_506|Compound:n9-isopropylolomoucine|FD-Score:2.93|P-value:6.77E-4 ID:SGTC_619|Compound:1652-1634|FD-Score:3.69|P-value:2.66E-5 ID:SGTC_719|Compound:2810-4230|FD-Score:-2.80|P-value:9.38E-4 ID:SGTC_845|Compound:0330-0138|FD-Score:3.27|P-value:1.69E-4 ID:SGTC_995|Compound:3931-2021|FD-Score:3.17|P-value:2.60E-4 ID:SGTC_1094|Compound:duloxetine|FD-Score:4.58|P-value:2.44E-7 ID:SGTC_1175|Compound:0741-0056|FD-Score:3.40|P-value:9.87E-5 ID:SGTC_1334|Compound:1486-0610|FD-Score:3.58|P-value:4.26E-5 ID:SGTC_1496|Compound:4469-0713|FD-Score:2.94|P-value:6.44E-4 ID:SGTC_1558|Compound:st069300|FD-Score:3.89|P-value:1.01E-5 ID:SGTC_1726|Compound:st035556|FD-Score:2.82|P-value:9.93E-4 ID:SGTC_1730|Compound:st033808|FD-Score:2.97|P-value:5.70E-4 ID:SGTC_1771|Compound:st048882|FD-Score:-3.49|P-value:5.56E-5 ID:SGTC_1797|Compound:st048695|FD-Score:5.68|P-value:2.22E-10 ID:SGTC_2042|Compound:p-Dimethylaminoacetophenone|FD-Score:3.48|P-value:6.76E-5 ID:SGTC_2050|Compound:5263056|FD-Score:3.31|P-value:1.44E-4 ID:SGTC_2054|Compound:5233173|FD-Score:-5.23|P-value:3.78E-9 ID:SGTC_2354|Compound:9060492|FD-Score:3.71|P-value:2.41E-5 ID:SGTC_2406|Compound:ethylparaben|FD-Score:3.16|P-value:2.65E-4 ID:SGTC_2482|Compound:5981709|FD-Score:-2.83|P-value:8.55E-4 ID:SGTC_2594|Compound:a-mangostin|FD-Score:3.48|P-value:6.86E-5 ID:SGTC_2882|Compound:9058044|FD-Score:-3.20|P-value:1.91E-4 ID:SGTC_2936|Compound:9038235|FD-Score:-3.34|P-value:1.08E-4 ID:SGTC_2937|Compound:9044753|FD-Score:3.01|P-value:4.89E-4 ID:SGTC_2972|Compound:9093619|FD-Score:4.17|P-value:2.40E-6 ID:SGTC_2975|Compound:9084847|FD-Score:-5.08|P-value:9.92E-9 ID:SGTC_3144|Compound:9087057|FD-Score:3.60|P-value:3.90E-5 ID:SGTC_3148|Compound:9097893|FD-Score:2.95|P-value:6.15E-4 ID:SGTC_3269|Compound:9137823|FD-Score:2.85|P-value:8.87E-4 ID:SGTC_10|Compound:0279-0212|FD-Score:3.18|P-value:2.52E-4 ID:SGTC_69|Compound:0096-0274|FD-Score:6.47|P-value:5.69E-13 ID:SGTC_105|Compound:0111-0055|FD-Score:2.96|P-value:6.06E-4 ID:SGTC_136|Compound:0097-0015|FD-Score:-2.82|P-value:8.68E-4 ID:SGTC_342|Compound:k048-0088|FD-Score:3.28|P-value:1.66E-4 ID:SGTC_506|Compound:n9-isopropylolomoucine|FD-Score:2.93|P-value:6.77E-4 ID:SGTC_619|Compound:1652-1634|FD-Score:3.69|P-value:2.66E-5 ID:SGTC_719|Compound:2810-4230|FD-Score:-2.80|P-value:9.38E-4 ID:SGTC_845|Compound:0330-0138|FD-Score:3.27|P-value:1.69E-4 ID:SGTC_995|Compound:3931-2021|FD-Score:3.17|P-value:2.60E-4 ID:SGTC_1094|Compound:duloxetine|FD-Score:4.58|P-value:2.44E-7 ID:SGTC_1175|Compound:0741-0056|FD-Score:3.40|P-value:9.87E-5 ID:SGTC_1334|Compound:1486-0610|FD-Score:3.58|P-value:4.26E-5 ID:SGTC_1496|Compound:4469-0713|FD-Score:2.94|P-value:6.44E-4 ID:SGTC_1558|Compound:st069300|FD-Score:3.89|P-value:1.01E-5 ID:SGTC_1726|Compound:st035556|FD-Score:2.82|P-value:9.93E-4 ID:SGTC_1730|Compound:st033808|FD-Score:2.97|P-value:5.70E-4 ID:SGTC_1771|Compound:st048882|FD-Score:-3.49|P-value:5.56E-5 ID:SGTC_1797|Compound:st048695|FD-Score:5.68|P-value:2.22E-10 ID:SGTC_2042|Compound:p-Dimethylaminoacetophenone|FD-Score:3.48|P-value:6.76E-5 ID:SGTC_2050|Compound:5263056|FD-Score:3.31|P-value:1.44E-4 ID:SGTC_2054|Compound:5233173|FD-Score:-5.23|P-value:3.78E-9 ID:SGTC_2354|Compound:9060492|FD-Score:3.71|P-value:2.41E-5 ID:SGTC_2406|Compound:ethylparaben|FD-Score:3.16|P-value:2.65E-4 ID:SGTC_2482|Compound:5981709|FD-Score:-2.83|P-value:8.55E-4 ID:SGTC_2594|Compound:a-mangostin|FD-Score:3.48|P-value:6.86E-5 ID:SGTC_2882|Compound:9058044|FD-Score:-3.20|P-value:1.91E-4 ID:SGTC_2936|Compound:9038235|FD-Score:-3.34|P-value:1.08E-4 ID:SGTC_2937|Compound:9044753|FD-Score:3.01|P-value:4.89E-4 ID:SGTC_2972|Compound:9093619|FD-Score:4.17|P-value:2.40E-6 ID:SGTC_2975|Compound:9084847|FD-Score:-5.08|P-value:9.92E-9 ID:SGTC_3144|Compound:9087057|FD-Score:3.60|P-value:3.90E-5 ID:SGTC_3148|Compound:9097893|FD-Score:2.95|P-value:6.15E-4 ID:SGTC_3269|Compound:9137823|FD-Score:2.85|P-value:8.87E-4

Top fitness defect scores for YBR087W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_69 0096-0274 6.47 206.7 µM 5.69E-13 mitochondrial processes 17 4.80E-11 0.03
2 SGTC_1797 st048695 5.68 56.8 µM 2.22E-10 NEO1 7 6.76E-9 0.67
3 SGTC_1094 duloxetine 4.58 25.4 µM 2.44E-7 RPP1 & pyrimidine depletion 2 2.28E-6 0.46
4 SGTC_2972 9093619 4.17 59.9 µM 2.40E-6 5 1.53E-5 0.17
5 SGTC_1558 st069300 3.89 45.6 µM 1.01E-5 2 5.07E-5 0.20
6 SGTC_2354 9060492 3.71 162.0 µM 2.41E-5 7 1.05E-4 0.03
7 SGTC_619 1652-1634 3.69 108.0 µM 2.66E-5 PDR1 7 1.14E-4 0.04
8 SGTC_3144 9087057 3.60 49.5 µM 3.90E-5 5 1.57E-4 0.06
9 SGTC_1334 1486-0610 3.58 236.0 µM 4.26E-5 60S ribosome export 7 1.69E-4 0.32
10 SGTC_2042 p-Dimethylaminoacetophenone 3.48 200.0 µM 6.76E-5 12 2.49E-4 0.08
11 SGTC_2594 a-mangostin 3.48 100.0 µM 6.86E-5 11 2.52E-4 0.03
12 SGTC_1175 0741-0056 3.40 27.6 µM 9.87E-5 9 3.41E-4 0.06
13 SGTC_2050 5263056 3.31 171.0 µM 1.44E-4 fatty acid desaturase (OLE1) 38 4.69E-4 0.11
14 SGTC_342 k048-0088 3.28 23.3 µM 1.66E-4 RSC & ERG11 18 5.27E-4 0.02
15 SGTC_845 0330-0138 3.27 4.5 µM 1.69E-4 12 5.34E-4 0.06
16 SGTC_10 0279-0212 3.18 42.6 µM 2.52E-4 TRP & mitochondrial translation 11 7.47E-4 0.03
17 SGTC_995 3931-2021 3.17 34.6 µM 2.60E-4 RPP1 & pyrimidine depletion 12 7.69E-4 0.11
18 SGTC_2406 ethylparaben 3.16 405.7 µM 2.65E-4 15 7.81E-4 0.06
19 SGTC_2937 9044753 3.01 11.3 µM 4.89E-4 60S ribosome export 18 0.00130 0.02
20 SGTC_1730 st033808 2.97 45.1 µM 5.70E-4 8 0.00148 0.03

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.183 1.52E-26 YKL122C SRP21 het Subunit of the signal recognition particle (SRP), which functions in protein targeting to the endoplasmic reticulum membrane; not found in mammalian SRP; forms a pre-SRP structure in the nucleolus that is translocated to the cytoplasm
0.120 3.42E-12 YDR331W GPI8 het ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog
0.119 4.91E-12 YPL243W SRP68 het Core component of the signal recognition particle (SRP) complex; SRP complex functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
0.110 1.70E-10 YER039C-A_p YER039C-A_p hom Putative protein of unknown function; YER039C-A is not an essential gene
0.108 3.48E-10 YBL035C POL12 het B subunit of DNA polymerase alpha-primase complex, required for initiation of DNA replication during mitotic and premeiotic DNA synthesis; also functions in telomere capping and length regulation
0.108 4.02E-10 YMR290W-A_d YMR290W-A_d het Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase
0.102 3.85E-9 YNL334C SNO2 hom Protein of unknown function, nearly identical to Sno3p; expression is induced before the diauxic shift and also in the absence of thiamin
0.100 6.44E-9 YDR352W YPQ2 hom Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; mutant phenotype is functionally complemented by rat PQLC2 vacuolar transporter
0.100 7.36E-9 YDR397C NCB2 het Subunit of a heterodimeric NC2 transcription regulator complex with Bur6p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2beta
0.098 1.21E-8 YDR365C ESF1 het Nucleolar protein involved in pre-rRNA processing; depletion causes severely decreased 18S rRNA levels
0.096 2.39E-8 YOR268C_p YOR268C_p hom Putative protein of unknown function; sporulation is abnormal in homozygous diploid; YOR268C is not an essential gene
0.095 4.07E-8 YPL213W LEA1 hom Component of U2 snRNP; disruption causes reduced U2 snRNP levels; physically interacts with Msl1p; putative homolog of human U2A' snRNP protein
0.094 5.65E-8 YKL139W CTK1 hom Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13
0.093 6.49E-8 YBL030C PET9 het Major ADP/ATP carrier of the mitochondrial inner membrane; exchanges cytosolic ADP for mitochondrially synthesized ATP; also imports heme and ATP; phosphorylated; required for viability in many lab strains that carry a sal1 mutation; PET9 has a paralog, AAC3, that arose from the whole genome duplication
0.093 6.79E-8 YDR037W KRS1 het Lysyl-tRNA synthetase