YBR098W / MMS4

Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR098W / MMS4

Click on Significant Values for Screen Details ID:SGTC_93|Compound:0109-0045|FD-Score:4.01|P-value:1.40E-5 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:3.60|P-value:8.56E-5 ID:SGTC_244|Compound:nsc-207895|FD-Score:13.40|P-value:9.18E-45 ID:SGTC_247|Compound:mechlorethamine|FD-Score:3.41|P-value:1.87E-4 ID:SGTC_248|Compound:streptozotocin|FD-Score:9.39|P-value:5.80E-23 ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:9.71|P-value:2.22E-24 ID:SGTC_268|Compound:camptothecin|FD-Score:12.40|P-value:2.91E-38 ID:SGTC_273|Compound:hydroxyurea|FD-Score:4.27|P-value:4.07E-6 ID:SGTC_479|Compound:n-linoleoylglycine|FD-Score:-3.78|P-value:4.16E-5 ID:SGTC_584|Compound:0986-0246|FD-Score:3.52|P-value:1.19E-4 ID:SGTC_745|Compound:0469-0705|FD-Score:3.70|P-value:5.54E-5 ID:SGTC_880|Compound:0833-0487|FD-Score:-3.89|P-value:2.56E-5 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:4.13|P-value:8.23E-6 ID:SGTC_1079|Compound:idarubicin|FD-Score:4.20|P-value:5.78E-6 ID:SGTC_1082|Compound:camptothecin|FD-Score:9.43|P-value:3.98E-23 ID:SGTC_1176|Compound:0848-0131|FD-Score:3.83|P-value:3.13E-5 ID:SGTC_1237|Compound:0330-0133|FD-Score:3.34|P-value:2.49E-4 ID:SGTC_1357|Compound:1503-1303|FD-Score:7.04|P-value:1.06E-13 ID:SGTC_1604|Compound:st002052|FD-Score:2.99|P-value:8.89E-4 ID:SGTC_1707|Compound:st032323|FD-Score:8.70|P-value:5.98E-20 ID:SGTC_1845|Compound:st056753|FD-Score:6.71|P-value:1.31E-12 ID:SGTC_1959|Compound:st077091|FD-Score:3.26|P-value:3.37E-4 ID:SGTC_2062|Compound:5245942|FD-Score:-3.33|P-value:2.67E-4 ID:SGTC_2148|Compound:5546263|FD-Score:3.85|P-value:2.91E-5 ID:SGTC_2612|Compound:dictamnine|FD-Score:7.94|P-value:6.35E-17 ID:SGTC_2633|Compound:5,7-dihydroxyisoflavone|FD-Score:-4.62|P-value:7.70E-7 ID:SGTC_2635|Compound:hydroquinidine|FD-Score:-3.59|P-value:9.57E-5 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:4.09|P-value:1.00E-5 ID:SGTC_2638|Compound:epoxomicin|FD-Score:3.53|P-value:1.13E-4 ID:SGTC_2671|Compound:danthron|FD-Score:3.05|P-value:7.21E-4 ID:SGTC_2738|Compound:aclarubicin|FD-Score:4.57|P-value:9.30E-7 ID:SGTC_2942|Compound:9052777|FD-Score:-2.98|P-value:9.76E-4 ID:SGTC_3214|Compound:9129694|FD-Score:3.21|P-value:4.05E-4 ID:SGTC_3220|Compound:9131269|FD-Score:-3.40|P-value:2.05E-4 ID:SGTC_3315|Compound:9136395|FD-Score:2.97|P-value:9.48E-4 ID:SGTC_93|Compound:0109-0045|FD-Score:4.01|P-value:1.40E-5 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:3.60|P-value:8.56E-5 ID:SGTC_244|Compound:nsc-207895|FD-Score:13.40|P-value:9.18E-45 ID:SGTC_247|Compound:mechlorethamine|FD-Score:3.41|P-value:1.87E-4 ID:SGTC_248|Compound:streptozotocin|FD-Score:9.39|P-value:5.80E-23 ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:9.71|P-value:2.22E-24 ID:SGTC_268|Compound:camptothecin|FD-Score:12.40|P-value:2.91E-38 ID:SGTC_273|Compound:hydroxyurea|FD-Score:4.27|P-value:4.07E-6 ID:SGTC_479|Compound:n-linoleoylglycine|FD-Score:-3.78|P-value:4.16E-5 ID:SGTC_584|Compound:0986-0246|FD-Score:3.52|P-value:1.19E-4 ID:SGTC_745|Compound:0469-0705|FD-Score:3.70|P-value:5.54E-5 ID:SGTC_880|Compound:0833-0487|FD-Score:-3.89|P-value:2.56E-5 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:4.13|P-value:8.23E-6 ID:SGTC_1079|Compound:idarubicin|FD-Score:4.20|P-value:5.78E-6 ID:SGTC_1082|Compound:camptothecin|FD-Score:9.43|P-value:3.98E-23 ID:SGTC_1176|Compound:0848-0131|FD-Score:3.83|P-value:3.13E-5 ID:SGTC_1237|Compound:0330-0133|FD-Score:3.34|P-value:2.49E-4 ID:SGTC_1357|Compound:1503-1303|FD-Score:7.04|P-value:1.06E-13 ID:SGTC_1604|Compound:st002052|FD-Score:2.99|P-value:8.89E-4 ID:SGTC_1707|Compound:st032323|FD-Score:8.70|P-value:5.98E-20 ID:SGTC_1845|Compound:st056753|FD-Score:6.71|P-value:1.31E-12 ID:SGTC_1959|Compound:st077091|FD-Score:3.26|P-value:3.37E-4 ID:SGTC_2062|Compound:5245942|FD-Score:-3.33|P-value:2.67E-4 ID:SGTC_2148|Compound:5546263|FD-Score:3.85|P-value:2.91E-5 ID:SGTC_2612|Compound:dictamnine|FD-Score:7.94|P-value:6.35E-17 ID:SGTC_2633|Compound:5,7-dihydroxyisoflavone|FD-Score:-4.62|P-value:7.70E-7 ID:SGTC_2635|Compound:hydroquinidine|FD-Score:-3.59|P-value:9.57E-5 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:4.09|P-value:1.00E-5 ID:SGTC_2638|Compound:epoxomicin|FD-Score:3.53|P-value:1.13E-4 ID:SGTC_2671|Compound:danthron|FD-Score:3.05|P-value:7.21E-4 ID:SGTC_2738|Compound:aclarubicin|FD-Score:4.57|P-value:9.30E-7 ID:SGTC_2942|Compound:9052777|FD-Score:-2.98|P-value:9.76E-4 ID:SGTC_3214|Compound:9129694|FD-Score:3.21|P-value:4.05E-4 ID:SGTC_3220|Compound:9131269|FD-Score:-3.40|P-value:2.05E-4 ID:SGTC_3315|Compound:9136395|FD-Score:2.97|P-value:9.48E-4

Top fitness defect scores for YBR098W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_244 nsc-207895 13.40 1.3 µM 9.18E-45 DNA damage response 2 2.40E-41
2 SGTC_268 camptothecin 12.40 39.6 µM 2.91E-38 DNA damage response 4 2.33E-35
3 SGTC_267 4-Nitroquinoline-1-oxide (4-NQO) 9.71 44.4 nM 2.22E-24 DNA damage response 15 1.42E-22
4 SGTC_1082 camptothecin 9.43 424.9 nM 3.98E-23 DNA damage response 5 2.02E-21
5 SGTC_248 streptozotocin 9.39 5.2 mM 5.80E-23 DNA damage response 2 2.86E-21
6 SGTC_1707 st032323 8.70 6.3 µM 5.98E-20 DNA damage response 13 1.71E-18
7 SGTC_2612 dictamnine 7.94 15.5 µM 6.35E-17 RNA pol III & RNase P/MRP 6 1.05E-15
8 SGTC_1357 1503-1303 7.04 39.5 µM 1.06E-13 4 9.77E-13
9 SGTC_1845 st056753 6.71 3.0 µM 1.31E-12 TSC3-RPN4 7 9.92E-12
10 SGTC_2738 aclarubicin 4.57 5.5 µM 9.30E-7 RNA pol III & RNase P/MRP 5 2.45E-6
11 SGTC_273 hydroxyurea 4.27 18.1 mM 4.07E-6 heme biosynthesis & mitochondrial translocase 27 9.57E-6
12 SGTC_1079 idarubicin 4.20 2.4 µM 5.78E-6 DNA damage response 17 1.32E-5
13 SGTC_915 methyl methanesulfonate (MMS) 4.13 110.0 mM 8.23E-6 DNA damage response 36 1.83E-5
14 SGTC_2636 apigenin dimethyl ether 4.09 100.0 µM 1.00E-5 42 2.20E-5
15 SGTC_93 0109-0045 4.01 59.2 µM 1.40E-5 DNA damage response 44 2.99E-5
16 SGTC_2148 5546263 3.85 113.6 µM 2.91E-5 21 5.87E-5
17 SGTC_1176 0848-0131 3.83 83.3 µM 3.13E-5 Golgi 27 6.28E-5
18 SGTC_745 0469-0705 3.70 296.0 µM 5.54E-5 mitochondrial processes 28 1.07E-4
19 SGTC_219 mitoxanthrone 3.60 164.4 µM 8.56E-5 DNA intercalators 61 1.59E-4
20 SGTC_2638 epoxomicin 3.53 18.0 µM 1.13E-4 47 2.06E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.666 0 YDR386W MUS81 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.597 1.38E-322 YBR099C_d YBR099C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4
0.551 8.52E-266 YDL059C RAD59 hom Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p
0.436 5.88E-156 YDR076W RAD55 hom Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p
0.423 1.83E-145 YJL092W SRS2 hom DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability
0.415 4.13E-140 YGL163C RAD54 hom DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress
0.415 1.38E-139 YDR004W RAD57 hom Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p
0.381 2.24E-116 YGL175C SAE2 hom Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents
0.354 1.74E-99 YJL047C RTT101 hom Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p
0.264 2.42E-54 YBL088C TEL1 hom Protein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; functionally redundant with Mec1p; regulates P-body formation induced by replication stress; homolog of human ataxia-telangiectasia mutated (ATM) gene, the gene responsible for ataxia telangiectasia (AT) (OMIM 607585)
0.260 6.54E-53 YML095C RAD10 hom Single-stranded DNA endonuclease (with Rad1p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein
0.247 5.47E-48 YGR258C RAD2 hom Single-stranded DNA endonuclease, cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein
0.245 5.67E-47 YMR201C RAD14 hom Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein
0.238 1.40E-44 YDR092W UBC13 hom Ubiquitin-conjugating enzyme involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus
0.235 3.54E-43 YER095W RAD51 hom Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein