Hsp70 (Ssa1p) nucleotide exchange factor; cytosolic homolog of Sil1p, which is the nucleotide exchange factor for BiP (Kar2p) in the endoplasmic reticulum; protein abundance increases in response to DNA replication stress
Zygosity: Homozygous strain
fixedexpanded
![]() ![]() Click on Significant Values for Screen Details |
Top fitness defect scores for YBR101C deletion by condition
Download Fitness data (tab-delimited text) (excel) |
Correlation | pval | ORF | Gene | Zygosity | Description |
---|---|---|---|---|---|
0.189 | 2.54E-28 | YNR043W | MVD1 | het | Mevalonate pyrophosphate decarboxylase, essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer |
0.124 | 6.60E-13 | YHR107C | CDC12 | het | Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells |
0.121 | 2.33E-12 | YDR269C_d | YDR269C_d | hom | Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data |
0.115 | 2.60E-11 | YDL129W | YDL129W | hom | Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; YDL129W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress |
0.112 | 7.52E-11 | YJL094C | KHA1 | hom | Putative K+/H+ antiporter with a probable role in intracellular cation homeostasis, localized to Golgi vesicles and detected in highly purified mitochondria in high-throughput studies |
0.109 | 2.64E-10 | YDR010C_d | YDR010C_d | hom | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data |
0.106 | 6.91E-10 | YLL024C | SSA2 | hom | ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shock protein 70 (HSP70) family; associated with the chaperonin-containing T-complex; present in the cytoplasm, vacuolar membrane and cell wall; 98% identical with paralog Ssa1p, but subtle differences between the two proteins provide functional specificity with respect to propagation of yeast [URE3] prions and vacuolar-mediated degradations of gluconeogenesis enzymes |
0.106 | 8.33E-10 | YHR139C | SPS100 | hom | Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall; expression also induced in cells treated with the mycotoxin patulin |
0.105 | 9.49E-10 | YEL060C | PRB1 | hom | Vacuolar proteinase B (yscB); serine protease of the subtilisin family; involved in protein degradation in the vacuole and required for full protein degradation during sporulation; activity inhibited by Pbi2p; protein abundance increases in response to DNA replication stress |
0.102 | 2.79E-9 | YDR270W | CCC2 | hom | Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism |
0.099 | 8.93E-9 | YHR023W | MYO1 | het | Type II myosin heavy chain, required for wild-type cytokinesis and cell separation; localizes to the actomyosin ring; binds to myosin light chains Mlc1p and Mlc2p through its IQ1 and IQ2 motifs respectively |
0.096 | 2.76E-8 | YNL102W | POL1 | het | Catalytic subunit of the DNA polymerase I alpha-primase complex, required for the initiation of DNA replication during mitotic DNA synthesis and premeiotic DNA synthesis |
0.095 | 3.58E-8 | YJR040W | GEF1 | hom | Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism |
0.095 | 3.90E-8 | YNL009W | IDP3 | hom | Peroxisomal NADP-dependent isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate with the formation of NADP(H+), required for growth on unsaturated fatty acids; IDP3 has a paralog, IDP2, that arose from the whole genome duplication |
0.089 | 2.20E-7 | YBR094W | PBY1 | hom | Putative tubulin tyrosine ligase associated with P-bodies; forms cytoplasmic foci upon DNA replication stress |