YBR101C / FES1

Hsp70 (Ssa1p) nucleotide exchange factor; cytosolic homolog of Sil1p, which is the nucleotide exchange factor for BiP (Kar2p) in the endoplasmic reticulum; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR101C / FES1

Click on Significant Values for Screen Details ID:SGTC_71|Compound:0417-1750|FD-Score:-3.08|P-value:9.95E-4 ID:SGTC_143|Compound:4032-1194|FD-Score:4.93|P-value:3.69E-7 ID:SGTC_151|Compound:3013-0144|FD-Score:9.42|P-value:1.49E-21 ID:SGTC_155|Compound:k072-0202|FD-Score:4.12|P-value:1.70E-5 ID:SGTC_157|Compound:1348-1695|FD-Score:5.54|P-value:1.30E-8 ID:SGTC_649|Compound:1123-0311|FD-Score:3.67|P-value:1.10E-4 ID:SGTC_683|Compound:panaxydol|FD-Score:3.47|P-value:2.46E-4 ID:SGTC_769|Compound:0302-0231|FD-Score:3.98|P-value:3.11E-5 ID:SGTC_889|Compound:0919-1524|FD-Score:4.15|P-value:1.51E-5 ID:SGTC_922|Compound:1866-0035|FD-Score:3.38|P-value:3.45E-4 ID:SGTC_973|Compound:1325-0077|FD-Score:3.89|P-value:4.53E-5 ID:SGTC_977|Compound:1348-1378|FD-Score:3.80|P-value:6.67E-5 ID:SGTC_1028|Compound:k015-0029|FD-Score:-3.34|P-value:3.94E-4 ID:SGTC_1129|Compound:3453-1384|FD-Score:3.41|P-value:3.04E-4 ID:SGTC_1185|Compound:1319-0113|FD-Score:3.20|P-value:6.50E-4 ID:SGTC_1254|Compound:0550-0035|FD-Score:3.43|P-value:2.78E-4 ID:SGTC_1256|Compound:0570-0116|FD-Score:4.42|P-value:4.33E-6 ID:SGTC_1259|Compound:0671-0132|FD-Score:4.19|P-value:1.27E-5 ID:SGTC_1321|Compound:1326-2144|FD-Score:3.85|P-value:5.35E-5 ID:SGTC_1369|Compound:1866-0067|FD-Score:3.47|P-value:2.44E-4 ID:SGTC_1838|Compound:st055369|FD-Score:-3.34|P-value:3.97E-4 ID:SGTC_1920|Compound:st075728|FD-Score:4.28|P-value:8.31E-6 ID:SGTC_2016|Compound:5121758|FD-Score:-3.51|P-value:2.12E-4 ID:SGTC_2082|Compound:5219693|FD-Score:-3.21|P-value:6.39E-4 ID:SGTC_2093|Compound:5331342|FD-Score:4.42|P-value:4.48E-6 ID:SGTC_2097|Compound:5376186|FD-Score:3.49|P-value:2.22E-4 ID:SGTC_2116|Compound:5511452|FD-Score:4.75|P-value:8.97E-7 ID:SGTC_2134|Compound:5324836|FD-Score:3.11|P-value:8.77E-4 ID:SGTC_2166|Compound:5933061|FD-Score:4.08|P-value:2.09E-5 ID:SGTC_2321|Compound:7390090|FD-Score:3.55|P-value:1.81E-4 ID:SGTC_2325|Compound:6158266|FD-Score:4.04|P-value:2.43E-5 ID:SGTC_2469|Compound:5655019|FD-Score:3.11|P-value:8.96E-4 ID:SGTC_2495|Compound:cafestol|FD-Score:-3.45|P-value:2.66E-4 ID:SGTC_2506|Compound:ethyl hematommate|FD-Score:-3.44|P-value:2.76E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:-3.08|P-value:9.76E-4 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:4.37|P-value:5.54E-6 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:-3.58|P-value:1.64E-4 ID:SGTC_2752|Compound:diclazuril|FD-Score:4.05|P-value:2.30E-5 ID:SGTC_2829|Compound:7992743|FD-Score:3.08|P-value:9.79E-4 ID:SGTC_2874|Compound:9043904|FD-Score:3.89|P-value:4.59E-5 ID:SGTC_3097|Compound:9117246|FD-Score:3.80|P-value:6.58E-5 ID:SGTC_3166|Compound:9102297|FD-Score:-3.25|P-value:5.45E-4 ID:SGTC_3173|Compound:9105693|FD-Score:-4.91|P-value:4.07E-7 ID:SGTC_3211|Compound:9128781|FD-Score:-3.26|P-value:5.21E-4 ID:SGTC_71|Compound:0417-1750|FD-Score:-3.08|P-value:9.95E-4 ID:SGTC_143|Compound:4032-1194|FD-Score:4.93|P-value:3.69E-7 ID:SGTC_151|Compound:3013-0144|FD-Score:9.42|P-value:1.49E-21 ID:SGTC_155|Compound:k072-0202|FD-Score:4.12|P-value:1.70E-5 ID:SGTC_157|Compound:1348-1695|FD-Score:5.54|P-value:1.30E-8 ID:SGTC_649|Compound:1123-0311|FD-Score:3.67|P-value:1.10E-4 ID:SGTC_683|Compound:panaxydol|FD-Score:3.47|P-value:2.46E-4 ID:SGTC_769|Compound:0302-0231|FD-Score:3.98|P-value:3.11E-5 ID:SGTC_889|Compound:0919-1524|FD-Score:4.15|P-value:1.51E-5 ID:SGTC_922|Compound:1866-0035|FD-Score:3.38|P-value:3.45E-4 ID:SGTC_973|Compound:1325-0077|FD-Score:3.89|P-value:4.53E-5 ID:SGTC_977|Compound:1348-1378|FD-Score:3.80|P-value:6.67E-5 ID:SGTC_1028|Compound:k015-0029|FD-Score:-3.34|P-value:3.94E-4 ID:SGTC_1129|Compound:3453-1384|FD-Score:3.41|P-value:3.04E-4 ID:SGTC_1185|Compound:1319-0113|FD-Score:3.20|P-value:6.50E-4 ID:SGTC_1254|Compound:0550-0035|FD-Score:3.43|P-value:2.78E-4 ID:SGTC_1256|Compound:0570-0116|FD-Score:4.42|P-value:4.33E-6 ID:SGTC_1259|Compound:0671-0132|FD-Score:4.19|P-value:1.27E-5 ID:SGTC_1321|Compound:1326-2144|FD-Score:3.85|P-value:5.35E-5 ID:SGTC_1369|Compound:1866-0067|FD-Score:3.47|P-value:2.44E-4 ID:SGTC_1838|Compound:st055369|FD-Score:-3.34|P-value:3.97E-4 ID:SGTC_1920|Compound:st075728|FD-Score:4.28|P-value:8.31E-6 ID:SGTC_2016|Compound:5121758|FD-Score:-3.51|P-value:2.12E-4 ID:SGTC_2082|Compound:5219693|FD-Score:-3.21|P-value:6.39E-4 ID:SGTC_2093|Compound:5331342|FD-Score:4.42|P-value:4.48E-6 ID:SGTC_2097|Compound:5376186|FD-Score:3.49|P-value:2.22E-4 ID:SGTC_2116|Compound:5511452|FD-Score:4.75|P-value:8.97E-7 ID:SGTC_2134|Compound:5324836|FD-Score:3.11|P-value:8.77E-4 ID:SGTC_2166|Compound:5933061|FD-Score:4.08|P-value:2.09E-5 ID:SGTC_2321|Compound:7390090|FD-Score:3.55|P-value:1.81E-4 ID:SGTC_2325|Compound:6158266|FD-Score:4.04|P-value:2.43E-5 ID:SGTC_2469|Compound:5655019|FD-Score:3.11|P-value:8.96E-4 ID:SGTC_2495|Compound:cafestol|FD-Score:-3.45|P-value:2.66E-4 ID:SGTC_2506|Compound:ethyl hematommate|FD-Score:-3.44|P-value:2.76E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:-3.08|P-value:9.76E-4 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:4.37|P-value:5.54E-6 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:-3.58|P-value:1.64E-4 ID:SGTC_2752|Compound:diclazuril|FD-Score:4.05|P-value:2.30E-5 ID:SGTC_2829|Compound:7992743|FD-Score:3.08|P-value:9.79E-4 ID:SGTC_2874|Compound:9043904|FD-Score:3.89|P-value:4.59E-5 ID:SGTC_3097|Compound:9117246|FD-Score:3.80|P-value:6.58E-5 ID:SGTC_3166|Compound:9102297|FD-Score:-3.25|P-value:5.45E-4 ID:SGTC_3173|Compound:9105693|FD-Score:-4.91|P-value:4.07E-7 ID:SGTC_3211|Compound:9128781|FD-Score:-3.26|P-value:5.21E-4

Top fitness defect scores for YBR101C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_151 3013-0144 9.42 2.3 µM 1.49E-21 iron homeostasis 2 2.35E-21
2 SGTC_157 1348-1695 5.54 65.8 µM 1.30E-8 iron homeostasis 23 1.53E-8
3 SGTC_143 4032-1194 4.93 15.7 µM 3.69E-7 32 4.21E-7
4 SGTC_2116 5511452 4.75 16.9 µM 8.97E-7 7 1.01E-6
5 SGTC_1256 0570-0116 4.42 285.0 µM 4.33E-6 11 4.82E-6
6 SGTC_2093 5331342 4.42 194.4 µM 4.48E-6 22 4.98E-6
7 SGTC_2572 astragaloside iv 4.37 100.0 µM 5.54E-6 15 6.15E-6
8 SGTC_1920 st075728 4.28 45.7 µM 8.31E-6 15 9.19E-6
9 SGTC_1259 0671-0132 4.19 43.0 µM 1.27E-5 21 1.39E-5
10 SGTC_889 0919-1524 4.15 82.7 µM 1.51E-5 10 1.66E-5
11 SGTC_155 k072-0202 4.12 43.6 µM 1.70E-5 15 1.87E-5
12 SGTC_2166 5933061 4.08 31.5 µM 2.09E-5 tubulin folding & SWR complex 40 2.29E-5
13 SGTC_2752 diclazuril 4.05 33.1 µM 2.30E-5 114 2.52E-5
14 SGTC_2325 6158266 4.04 200.0 µM 2.43E-5 21 2.66E-5
15 SGTC_769 0302-0231 3.98 76.0 µM 3.11E-5 39 3.39E-5
16 SGTC_973 1325-0077 3.89 65.9 µM 4.53E-5 8 4.93E-5
17 SGTC_2874 9043904 3.89 71.4 µM 4.59E-5 13 4.99E-5
18 SGTC_1321 1326-2144 3.85 215.0 µM 5.35E-5 12 5.81E-5
19 SGTC_3097 9117246 3.80 49.5 µM 6.58E-5 12 7.14E-5
20 SGTC_977 1348-1378 3.80 135.0 µM 6.67E-5 26 7.23E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.189 2.54E-28 YNR043W MVD1 het Mevalonate pyrophosphate decarboxylase, essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer
0.124 6.60E-13 YHR107C CDC12 het Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
0.121 2.33E-12 YDR269C_d YDR269C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.115 2.60E-11 YDL129W YDL129W hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; YDL129W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress
0.112 7.52E-11 YJL094C KHA1 hom Putative K+/H+ antiporter with a probable role in intracellular cation homeostasis, localized to Golgi vesicles and detected in highly purified mitochondria in high-throughput studies
0.109 2.64E-10 YDR010C_d YDR010C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.106 6.91E-10 YLL024C SSA2 hom ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shock protein 70 (HSP70) family; associated with the chaperonin-containing T-complex; present in the cytoplasm, vacuolar membrane and cell wall; 98% identical with paralog Ssa1p, but subtle differences between the two proteins provide functional specificity with respect to propagation of yeast [URE3] prions and vacuolar-mediated degradations of gluconeogenesis enzymes
0.106 8.33E-10 YHR139C SPS100 hom Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall; expression also induced in cells treated with the mycotoxin patulin
0.105 9.49E-10 YEL060C PRB1 hom Vacuolar proteinase B (yscB); serine protease of the subtilisin family; involved in protein degradation in the vacuole and required for full protein degradation during sporulation; activity inhibited by Pbi2p; protein abundance increases in response to DNA replication stress
0.102 2.79E-9 YDR270W CCC2 hom Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism
0.099 8.93E-9 YHR023W MYO1 het Type II myosin heavy chain, required for wild-type cytokinesis and cell separation; localizes to the actomyosin ring; binds to myosin light chains Mlc1p and Mlc2p through its IQ1 and IQ2 motifs respectively
0.096 2.76E-8 YNL102W POL1 het Catalytic subunit of the DNA polymerase I alpha-primase complex, required for the initiation of DNA replication during mitotic DNA synthesis and premeiotic DNA synthesis
0.095 3.58E-8 YJR040W GEF1 hom Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism
0.095 3.90E-8 YNL009W IDP3 hom Peroxisomal NADP-dependent isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate with the formation of NADP(H+), required for growth on unsaturated fatty acids; IDP3 has a paralog, IDP2, that arose from the whole genome duplication
0.089 2.20E-7 YBR094W PBY1 hom Putative tubulin tyrosine ligase associated with P-bodies; forms cytoplasmic foci upon DNA replication stress