HIPHOP chemogenomics database

YBR101C / FES1

Hsp70 (Ssa1p) nucleotide exchange factor; cytosolic homolog of Sil1p, which is the nucleotide exchange factor for BiP (Kar2p) in the endoplasmic reticulum; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain

fixedexpanded
Profile for YBR101C / FES1

Click on Significant Values for Screen Details

Top fitness defect scores for YBR101C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_151	3013-0144	9.42	2.3 µM	1.49E-21	iron homeostasis	2	2.35E-21
2	SGTC_157	1348-1695	5.54	65.8 µM	1.30E-8	iron homeostasis	23	1.53E-8
3	SGTC_143	4032-1194	4.93	15.7 µM	3.69E-7		32	4.21E-7
4	SGTC_2116	5511452	4.75	16.9 µM	8.97E-7		7	1.01E-6
5	SGTC_1256	0570-0116	4.42	285.0 µM	4.33E-6		11	4.82E-6
6	SGTC_2093	5331342	4.42	194.4 µM	4.48E-6		22	4.98E-6
7	SGTC_2572	astragaloside iv	4.37	100.0 µM	5.54E-6		15	6.15E-6
8	SGTC_1920	st075728	4.28	45.7 µM	8.31E-6		15	9.19E-6
9	SGTC_1259	0671-0132	4.19	43.0 µM	1.27E-5		21	1.39E-5
10	SGTC_889	0919-1524	4.15	82.7 µM	1.51E-5		10	1.66E-5
11	SGTC_155	k072-0202	4.12	43.6 µM	1.70E-5		15	1.87E-5
12	SGTC_2166	5933061	4.08	31.5 µM	2.09E-5	tubulin folding & SWR complex	40	2.29E-5
13	SGTC_2752	diclazuril	4.05	33.1 µM	2.30E-5		114	2.52E-5
14	SGTC_2325	6158266	4.04	200.0 µM	2.43E-5		21	2.66E-5
15	SGTC_769	0302-0231	3.98	76.0 µM	3.11E-5		39	3.39E-5
16	SGTC_973	1325-0077	3.89	65.9 µM	4.53E-5		8	4.93E-5
17	SGTC_2874	9043904	3.89	71.4 µM	4.59E-5		13	4.99E-5
18	SGTC_1321	1326-2144	3.85	215.0 µM	5.35E-5		12	5.81E-5
19	SGTC_3097	9117246	3.80	49.5 µM	6.58E-5		12	7.14E-5
20	SGTC_977	1348-1378	3.80	135.0 µM	6.67E-5		26	7.23E-5

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.189	2.54E-28	YNR043W	MVD1	het	Mevalonate pyrophosphate decarboxylase, essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer
0.124	6.60E-13	YHR107C	CDC12	het	Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
0.121	2.33E-12	YDR269C_d	YDR269C_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.115	2.60E-11	YDL129W	YDL129W	hom	Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; YDL129W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress
0.112	7.52E-11	YJL094C	KHA1	hom	Putative K+/H+ antiporter with a probable role in intracellular cation homeostasis, localized to Golgi vesicles and detected in highly purified mitochondria in high-throughput studies
0.109	2.64E-10	YDR010C_d	YDR010C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.106	6.91E-10	YLL024C	SSA2	hom	ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shock protein 70 (HSP70) family; associated with the chaperonin-containing T-complex; present in the cytoplasm, vacuolar membrane and cell wall; 98% identical with paralog Ssa1p, but subtle differences between the two proteins provide functional specificity with respect to propagation of yeast [URE3] prions and vacuolar-mediated degradations of gluconeogenesis enzymes
0.106	8.33E-10	YHR139C	SPS100	hom	Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall; expression also induced in cells treated with the mycotoxin patulin
0.105	9.49E-10	YEL060C	PRB1	hom	Vacuolar proteinase B (yscB); serine protease of the subtilisin family; involved in protein degradation in the vacuole and required for full protein degradation during sporulation; activity inhibited by Pbi2p; protein abundance increases in response to DNA replication stress
0.102	2.79E-9	YDR270W	CCC2	hom	Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism
0.099	8.93E-9	YHR023W	MYO1	het	Type II myosin heavy chain, required for wild-type cytokinesis and cell separation; localizes to the actomyosin ring; binds to myosin light chains Mlc1p and Mlc2p through its IQ1 and IQ2 motifs respectively
0.096	2.76E-8	YNL102W	POL1	het	Catalytic subunit of the DNA polymerase I alpha-primase complex, required for the initiation of DNA replication during mitotic DNA synthesis and premeiotic DNA synthesis
0.095	3.58E-8	YJR040W	GEF1	hom	Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism
0.095	3.90E-8	YNL009W	IDP3	hom	Peroxisomal NADP-dependent isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate with the formation of NADP(H+), required for growth on unsaturated fatty acids; IDP3 has a paralog, IDP2, that arose from the whole genome duplication
0.089	2.20E-7	YBR094W	PBY1	hom	Putative tubulin tyrosine ligase associated with P-bodies; forms cytoplasmic foci upon DNA replication stress