YBR102C / EXO84

Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate

Zygosity: Heterozygous strain
fixedexpanded
Profile for YBR102C / EXO84

Click on Significant Values for Screen Details ID:SGTC_57|Compound:miconazole|FD-Score:10.40|P-value:6.43E-31 ID:SGTC_77|Compound:0828-0286|FD-Score:-3.43|P-value:7.40E-5 ID:SGTC_171|Compound:k064-0041|FD-Score:3.13|P-value:2.57E-4 ID:SGTC_178|Compound:4092-0806|FD-Score:3.52|P-value:4.71E-5 ID:SGTC_252|Compound:miconazole|FD-Score:9.44|P-value:6.59E-26 ID:SGTC_256|Compound:clotrimazole|FD-Score:4.76|P-value:6.58E-8 ID:SGTC_270|Compound:tamoxifen|FD-Score:3.06|P-value:3.47E-4 ID:SGTC_291|Compound:4130-1278|FD-Score:4.02|P-value:4.12E-6 ID:SGTC_305|Compound:1682-2595|FD-Score:2.91|P-value:6.16E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:12.70|P-value:4.36E-45 ID:SGTC_320|Compound:4092-0846|FD-Score:7.13|P-value:1.36E-15 ID:SGTC_327|Compound:1273-0057|FD-Score:4.33|P-value:8.10E-7 ID:SGTC_333|Compound:3486-0131|FD-Score:2.95|P-value:5.43E-4 ID:SGTC_407|Compound:eburnamonine|FD-Score:3.70|P-value:2.09E-5 ID:SGTC_589|Compound:1227-0070|FD-Score:-4.18|P-value:1.84E-6 ID:SGTC_724|Compound:3910-0327|FD-Score:2.83|P-value:8.43E-4 ID:SGTC_764|Compound:1951-2361|FD-Score:-2.87|P-value:7.55E-4 ID:SGTC_775|Compound:4577-0580|FD-Score:3.24|P-value:1.62E-4 ID:SGTC_842|Compound:0271-0008|FD-Score:3.65|P-value:2.60E-5 ID:SGTC_844|Compound:0330-0132|FD-Score:3.07|P-value:3.27E-4 ID:SGTC_846|Compound:0335-0850|FD-Score:3.81|P-value:1.21E-5 ID:SGTC_951|Compound:1171-0579|FD-Score:-3.74|P-value:1.73E-5 ID:SGTC_1066|Compound:ketoconazole|FD-Score:4.20|P-value:1.61E-6 ID:SGTC_1072|Compound:oxiconazole|FD-Score:5.70|P-value:1.30E-10 ID:SGTC_1082|Compound:camptothecin|FD-Score:-3.40|P-value:8.49E-5 ID:SGTC_1139|Compound:k015-0024|FD-Score:3.31|P-value:1.24E-4 ID:SGTC_1170|Compound:0310-0127|FD-Score:4.10|P-value:2.75E-6 ID:SGTC_1207|Compound:2099-0101|FD-Score:3.19|P-value:2.05E-4 ID:SGTC_1209|Compound:2323-2347|FD-Score:3.31|P-value:1.20E-4 ID:SGTC_1255|Compound:0566-0045|FD-Score:2.88|P-value:7.05E-4 ID:SGTC_1276|Compound:0871-0068|FD-Score:3.90|P-value:7.53E-6 ID:SGTC_1300|Compound:1094-0077|FD-Score:3.14|P-value:2.55E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.14|P-value:2.56E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:-3.03|P-value:3.92E-4 ID:SGTC_1524|Compound:corynanthine|FD-Score:3.05|P-value:3.55E-4 ID:SGTC_1567|Compound:st069303|FD-Score:4.42|P-value:4.88E-7 ID:SGTC_1568|Compound:digitonin|FD-Score:3.21|P-value:1.85E-4 ID:SGTC_1665|Compound:st014639|FD-Score:-3.01|P-value:4.33E-4 ID:SGTC_1679|Compound:4130-1276|FD-Score:5.92|P-value:2.62E-11 ID:SGTC_1787|Compound:fluconazole|FD-Score:-2.82|P-value:9.12E-4 ID:SGTC_1886|Compound:st057356|FD-Score:3.67|P-value:2.40E-5 ID:SGTC_1887|Compound:st057648|FD-Score:4.43|P-value:4.50E-7 ID:SGTC_1899|Compound:st063254|FD-Score:-7.46|P-value:6.79E-17 ID:SGTC_1902|Compound:st060212|FD-Score:-5.91|P-value:3.06E-11 ID:SGTC_2015|Compound:5119600|FD-Score:2.99|P-value:4.61E-4 ID:SGTC_2056|Compound:5235370|FD-Score:4.24|P-value:1.30E-6 ID:SGTC_2083|Compound:5306046|FD-Score:-3.35|P-value:1.02E-4 ID:SGTC_2087|Compound:5276107|FD-Score:-3.47|P-value:5.97E-5 ID:SGTC_2089|Compound:5281248|FD-Score:-3.47|P-value:6.04E-5 ID:SGTC_2249|Compound:haloperidol|FD-Score:3.98|P-value:5.20E-6 ID:SGTC_2250|Compound:alverine citrate|FD-Score:2.94|P-value:5.63E-4 ID:SGTC_2355|Compound:9061122|FD-Score:3.27|P-value:1.44E-4 ID:SGTC_2356|Compound:9061756|FD-Score:3.74|P-value:1.71E-5 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:3.59|P-value:3.52E-5 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:3.63|P-value:2.87E-5 ID:SGTC_2620|Compound:irigenin|FD-Score:4.25|P-value:1.25E-6 ID:SGTC_2703|Compound:st077852|FD-Score:-3.99|P-value:5.01E-6 ID:SGTC_2708|Compound:st078559|FD-Score:3.26|P-value:1.52E-4 ID:SGTC_2778|Compound:tioconazole|FD-Score:4.11|P-value:2.55E-6 ID:SGTC_2814|Compound:7977482|FD-Score:3.81|P-value:1.18E-5 ID:SGTC_2831|Compound:7999036|FD-Score:3.21|P-value:1.89E-4 ID:SGTC_2852|Compound:9023854|FD-Score:2.83|P-value:8.49E-4 ID:SGTC_2910|Compound:7948405|FD-Score:2.85|P-value:7.93E-4 ID:SGTC_2930|Compound:9013230|FD-Score:3.56|P-value:4.05E-5 ID:SGTC_3103|Compound:9120822|FD-Score:3.33|P-value:1.13E-4 ID:SGTC_3152|Compound:9099253|FD-Score:3.51|P-value:5.00E-5 ID:SGTC_3162|Compound:9099653|FD-Score:3.28|P-value:1.37E-4 ID:SGTC_3214|Compound:9129694|FD-Score:3.12|P-value:2.72E-4 ID:SGTC_57|Compound:miconazole|FD-Score:10.40|P-value:6.43E-31 ID:SGTC_77|Compound:0828-0286|FD-Score:-3.43|P-value:7.40E-5 ID:SGTC_171|Compound:k064-0041|FD-Score:3.13|P-value:2.57E-4 ID:SGTC_178|Compound:4092-0806|FD-Score:3.52|P-value:4.71E-5 ID:SGTC_252|Compound:miconazole|FD-Score:9.44|P-value:6.59E-26 ID:SGTC_256|Compound:clotrimazole|FD-Score:4.76|P-value:6.58E-8 ID:SGTC_270|Compound:tamoxifen|FD-Score:3.06|P-value:3.47E-4 ID:SGTC_291|Compound:4130-1278|FD-Score:4.02|P-value:4.12E-6 ID:SGTC_305|Compound:1682-2595|FD-Score:2.91|P-value:6.16E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:12.70|P-value:4.36E-45 ID:SGTC_320|Compound:4092-0846|FD-Score:7.13|P-value:1.36E-15 ID:SGTC_327|Compound:1273-0057|FD-Score:4.33|P-value:8.10E-7 ID:SGTC_333|Compound:3486-0131|FD-Score:2.95|P-value:5.43E-4 ID:SGTC_407|Compound:eburnamonine|FD-Score:3.70|P-value:2.09E-5 ID:SGTC_589|Compound:1227-0070|FD-Score:-4.18|P-value:1.84E-6 ID:SGTC_724|Compound:3910-0327|FD-Score:2.83|P-value:8.43E-4 ID:SGTC_764|Compound:1951-2361|FD-Score:-2.87|P-value:7.55E-4 ID:SGTC_775|Compound:4577-0580|FD-Score:3.24|P-value:1.62E-4 ID:SGTC_842|Compound:0271-0008|FD-Score:3.65|P-value:2.60E-5 ID:SGTC_844|Compound:0330-0132|FD-Score:3.07|P-value:3.27E-4 ID:SGTC_846|Compound:0335-0850|FD-Score:3.81|P-value:1.21E-5 ID:SGTC_951|Compound:1171-0579|FD-Score:-3.74|P-value:1.73E-5 ID:SGTC_1066|Compound:ketoconazole|FD-Score:4.20|P-value:1.61E-6 ID:SGTC_1072|Compound:oxiconazole|FD-Score:5.70|P-value:1.30E-10 ID:SGTC_1082|Compound:camptothecin|FD-Score:-3.40|P-value:8.49E-5 ID:SGTC_1139|Compound:k015-0024|FD-Score:3.31|P-value:1.24E-4 ID:SGTC_1170|Compound:0310-0127|FD-Score:4.10|P-value:2.75E-6 ID:SGTC_1207|Compound:2099-0101|FD-Score:3.19|P-value:2.05E-4 ID:SGTC_1209|Compound:2323-2347|FD-Score:3.31|P-value:1.20E-4 ID:SGTC_1255|Compound:0566-0045|FD-Score:2.88|P-value:7.05E-4 ID:SGTC_1276|Compound:0871-0068|FD-Score:3.90|P-value:7.53E-6 ID:SGTC_1300|Compound:1094-0077|FD-Score:3.14|P-value:2.55E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.14|P-value:2.56E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:-3.03|P-value:3.92E-4 ID:SGTC_1524|Compound:corynanthine|FD-Score:3.05|P-value:3.55E-4 ID:SGTC_1567|Compound:st069303|FD-Score:4.42|P-value:4.88E-7 ID:SGTC_1568|Compound:digitonin|FD-Score:3.21|P-value:1.85E-4 ID:SGTC_1665|Compound:st014639|FD-Score:-3.01|P-value:4.33E-4 ID:SGTC_1679|Compound:4130-1276|FD-Score:5.92|P-value:2.62E-11 ID:SGTC_1787|Compound:fluconazole|FD-Score:-2.82|P-value:9.12E-4 ID:SGTC_1886|Compound:st057356|FD-Score:3.67|P-value:2.40E-5 ID:SGTC_1887|Compound:st057648|FD-Score:4.43|P-value:4.50E-7 ID:SGTC_1899|Compound:st063254|FD-Score:-7.46|P-value:6.79E-17 ID:SGTC_1902|Compound:st060212|FD-Score:-5.91|P-value:3.06E-11 ID:SGTC_2015|Compound:5119600|FD-Score:2.99|P-value:4.61E-4 ID:SGTC_2056|Compound:5235370|FD-Score:4.24|P-value:1.30E-6 ID:SGTC_2083|Compound:5306046|FD-Score:-3.35|P-value:1.02E-4 ID:SGTC_2087|Compound:5276107|FD-Score:-3.47|P-value:5.97E-5 ID:SGTC_2089|Compound:5281248|FD-Score:-3.47|P-value:6.04E-5 ID:SGTC_2249|Compound:haloperidol|FD-Score:3.98|P-value:5.20E-6 ID:SGTC_2250|Compound:alverine citrate|FD-Score:2.94|P-value:5.63E-4 ID:SGTC_2355|Compound:9061122|FD-Score:3.27|P-value:1.44E-4 ID:SGTC_2356|Compound:9061756|FD-Score:3.74|P-value:1.71E-5 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:3.59|P-value:3.52E-5 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:3.63|P-value:2.87E-5 ID:SGTC_2620|Compound:irigenin|FD-Score:4.25|P-value:1.25E-6 ID:SGTC_2703|Compound:st077852|FD-Score:-3.99|P-value:5.01E-6 ID:SGTC_2708|Compound:st078559|FD-Score:3.26|P-value:1.52E-4 ID:SGTC_2778|Compound:tioconazole|FD-Score:4.11|P-value:2.55E-6 ID:SGTC_2814|Compound:7977482|FD-Score:3.81|P-value:1.18E-5 ID:SGTC_2831|Compound:7999036|FD-Score:3.21|P-value:1.89E-4 ID:SGTC_2852|Compound:9023854|FD-Score:2.83|P-value:8.49E-4 ID:SGTC_2910|Compound:7948405|FD-Score:2.85|P-value:7.93E-4 ID:SGTC_2930|Compound:9013230|FD-Score:3.56|P-value:4.05E-5 ID:SGTC_3103|Compound:9120822|FD-Score:3.33|P-value:1.13E-4 ID:SGTC_3152|Compound:9099253|FD-Score:3.51|P-value:5.00E-5 ID:SGTC_3162|Compound:9099653|FD-Score:3.28|P-value:1.37E-4 ID:SGTC_3214|Compound:9129694|FD-Score:3.12|P-value:2.72E-4

Top fitness defect scores for YBR102C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_312 4092-0653 12.70 6.8 µM 4.36E-45 ergosterol depletion effects on membrane 4 4.23E-37 1.98
2 SGTC_57 miconazole 10.40 162.5 nM 6.43E-31 ergosterol depletion effects on membrane 5 1.53E-25 0.41
3 SGTC_252 miconazole 9.44 1.3 nM 6.59E-26 ergosterol depletion effects on membrane 5 1.87E-21 1.29
4 SGTC_320 4092-0846 7.13 5.3 µM 1.36E-15 ergosterol depletion effects on membrane 6 4.96E-13 0.86
5 SGTC_1679 4130-1276 5.92 6.7 µM 2.62E-11 Golgi 14 1.58E-9 0.11
6 SGTC_1072 oxiconazole 5.70 14.9 nM 1.30E-10 ergosterol depletion effects on membrane 10 5.87E-9 0.06
7 SGTC_256 clotrimazole 4.76 625.0 nM 6.58E-8 ergosterol depletion effects on membrane 13 9.64E-7 0.22
8 SGTC_1887 st057648 4.43 70.8 µM 4.50E-7 3 4.67E-6 0.33
9 SGTC_1567 st069303 4.42 68.4 µM 4.88E-7 mitochondrial processes 9 4.99E-6 0.06
10 SGTC_327 1273-0057 4.33 4.0 µM 8.10E-7 azole & statin 7 7.56E-6 0.02
11 SGTC_2620 irigenin 4.25 100.0 µM 1.25E-6 12 1.08E-5 0.21
12 SGTC_2056 5235370 4.24 200.0 µM 1.30E-6 4 1.12E-5 0.04
13 SGTC_1066 ketoconazole 4.20 628.0 nM 1.61E-6 1 1.33E-5 0.37
14 SGTC_2778 tioconazole 4.11 65.1 nM 2.55E-6 ergosterol depletion effects on membrane 26 1.94E-5 0.05
15 SGTC_1170 0310-0127 4.10 64.8 µM 2.75E-6 7 2.06E-5 0.10
16 SGTC_291 4130-1278 4.02 35.5 µM 4.12E-6 Golgi 5 2.88E-5 0.03
17 SGTC_2249 haloperidol 3.98 116.4 µM 5.20E-6 ergosterol depletion effects on membrane 42 3.48E-5 0.09
18 SGTC_1276 0871-0068 3.90 94.2 µM 7.53E-6 7 4.72E-5 0.10
19 SGTC_2814 7977482 3.81 43.0 µM 1.18E-5 13 6.84E-5 0.10
20 SGTC_846 0335-0850 3.81 148.0 µM 1.21E-5 ubiquinone biosynthesis & proteasome 6 6.99E-5 0.37

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.478 7.64E-191 YPR055W SEC8 het Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress
0.409 2.24E-135 YIL068C SEC6 het Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p
0.405 1.04E-132 YGL233W SEC15 het Essential 113 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; interacts with and functions as a downstream effector of active, GTP-bound Sec4p, a Rab family GTPase
0.330 5.02E-86 YMR218C TRS130 het One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in ER to Golgi membrane traffic; mutation activates transcription of OCH1; mutants are defective in cytoplasm-to-vacuole targeting (Cvt) pathway and starvation-induced autophagy
0.319 6.40E-80 YER008C SEC3 het Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress
0.297 3.29E-69 YDR166C SEC5 het Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip
0.280 2.89E-61 YFL034W_p YFL034W_p hom Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk
0.263 4.03E-54 YLR169W_d YLR169W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.254 1.26E-50 YFL005W SEC4 het Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane
0.250 6.24E-49 YBL010C_p YBL010C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles
0.232 4.37E-42 YLR170C APS1 hom Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex
0.231 8.76E-42 YJL085W EXO70 het Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.226 4.67E-40 YMR071C TVP18 hom Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments
0.218 1.92E-37 YAL038W CDC19 het Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication
0.210 6.93E-35 YPL259C APM1 hom Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved in clathrin-dependent Golgi protein sorting