YBR114W / RAD16

Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR114W / RAD16

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:-4.71|P-value:1.24E-6 ID:SGTC_72|Compound:0744-0168|FD-Score:5.05|P-value:2.87E-7 ID:SGTC_73|Compound:0327-0325|FD-Score:5.32|P-value:6.93E-8 ID:SGTC_120|Compound:rhodamine 6G|FD-Score:-4.10|P-value:2.03E-5 ID:SGTC_121|Compound:0557-0495|FD-Score:-4.95|P-value:3.81E-7 ID:SGTC_135|Compound:0933-0004|FD-Score:6.81|P-value:7.60E-12 ID:SGTC_137|Compound:0438-0332|FD-Score:-4.78|P-value:8.75E-7 ID:SGTC_138|Compound:0850-0259|FD-Score:-3.82|P-value:6.59E-5 ID:SGTC_140|Compound:0062-0056|FD-Score:4.34|P-value:8.94E-6 ID:SGTC_141|Compound:0443-0274|FD-Score:-3.53|P-value:2.03E-4 ID:SGTC_213|Compound:1246-0773|FD-Score:4.58|P-value:2.91E-6 ID:SGTC_361|Compound:000s-0510|FD-Score:-3.29|P-value:4.88E-4 ID:SGTC_366|Compound:0181-0206|FD-Score:-3.22|P-value:6.21E-4 ID:SGTC_412|Compound:gemfibrozil|FD-Score:3.75|P-value:1.04E-4 ID:SGTC_527|Compound:4476-4215|FD-Score:7.66|P-value:1.50E-14 ID:SGTC_532|Compound:1548-0774|FD-Score:5.99|P-value:1.44E-9 ID:SGTC_534|Compound:k292-0788|FD-Score:4.09|P-value:2.60E-5 ID:SGTC_536|Compound:3852-0578|FD-Score:3.24|P-value:6.98E-4 ID:SGTC_541|Compound:4513-1321|FD-Score:4.56|P-value:3.23E-6 ID:SGTC_565|Compound:1418-0001|FD-Score:-4.02|P-value:2.96E-5 ID:SGTC_579|Compound:4491-1009|FD-Score:3.31|P-value:5.35E-4 ID:SGTC_865|Compound:0488-0499|FD-Score:-3.13|P-value:8.47E-4 ID:SGTC_869|Compound:0564-0080|FD-Score:3.67|P-value:1.43E-4 ID:SGTC_1024|Compound:4507-0395|FD-Score:3.54|P-value:2.34E-4 ID:SGTC_1027|Compound:k015-0025|FD-Score:5.24|P-value:1.04E-7 ID:SGTC_1035|Compound:k081-0041|FD-Score:-4.37|P-value:6.17E-6 ID:SGTC_1038|Compound:k292-0204|FD-Score:3.26|P-value:6.51E-4 ID:SGTC_1150|Compound:3909-7983|FD-Score:4.01|P-value:3.72E-5 ID:SGTC_1175|Compound:0741-0056|FD-Score:3.92|P-value:5.40E-5 ID:SGTC_1227|Compound:3909-8010|FD-Score:-3.12|P-value:8.92E-4 ID:SGTC_1247|Compound:0448-0043|FD-Score:3.16|P-value:9.10E-4 ID:SGTC_1264|Compound:0783-0040|FD-Score:3.77|P-value:9.73E-5 ID:SGTC_1584|Compound:6-fluoro-dl-tryptophan|FD-Score:-3.63|P-value:1.39E-4 ID:SGTC_1699|Compound:st031318|FD-Score:3.90|P-value:5.73E-5 ID:SGTC_1744|Compound:st037937|FD-Score:3.18|P-value:8.36E-4 ID:SGTC_1865|Compound:st057639|FD-Score:3.42|P-value:3.69E-4 ID:SGTC_1907|Compound:st060210|FD-Score:3.16|P-value:9.09E-4 ID:SGTC_1980|Compound:st071811|FD-Score:3.21|P-value:7.57E-4 ID:SGTC_2008|Compound:4012253|FD-Score:-3.60|P-value:1.58E-4 ID:SGTC_2019|Compound:4016004|FD-Score:5.44|P-value:3.63E-8 ID:SGTC_2136|Compound:5328288|FD-Score:-3.41|P-value:3.23E-4 ID:SGTC_2240|Compound:6690706|FD-Score:3.82|P-value:7.93E-5 ID:SGTC_2241|Compound:6772625|FD-Score:4.90|P-value:6.03E-7 ID:SGTC_2317|Compound:5986382|FD-Score:3.19|P-value:8.29E-4 ID:SGTC_2379|Compound:9035213|FD-Score:-3.13|P-value:8.54E-4 ID:SGTC_2401|Compound:5128683|FD-Score:-3.25|P-value:5.67E-4 ID:SGTC_2406|Compound:ethylparaben|FD-Score:-3.50|P-value:2.32E-4 ID:SGTC_2564|Compound:betulinic acid|FD-Score:5.21|P-value:1.26E-7 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:6.90|P-value:3.90E-12 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:5.03|P-value:3.09E-7 ID:SGTC_2634|Compound:4'-methoxyflavone|FD-Score:-3.66|P-value:1.24E-4 ID:SGTC_2640|Compound:pentamidine|FD-Score:-5.29|P-value:6.45E-8 ID:SGTC_2837|Compound:9008476|FD-Score:3.30|P-value:5.65E-4 ID:SGTC_2913|Compound:7966556|FD-Score:3.54|P-value:2.34E-4 ID:SGTC_2917|Compound:7979103|FD-Score:3.59|P-value:1.93E-4 ID:SGTC_2921|Compound:7974240|FD-Score:-3.22|P-value:6.26E-4 ID:SGTC_3059|Compound:1327-0034|FD-Score:3.15|P-value:9.42E-4 ID:SGTC_3341|Compound:9147222|FD-Score:3.65|P-value:1.54E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-4.71|P-value:1.24E-6 ID:SGTC_72|Compound:0744-0168|FD-Score:5.05|P-value:2.87E-7 ID:SGTC_73|Compound:0327-0325|FD-Score:5.32|P-value:6.93E-8 ID:SGTC_120|Compound:rhodamine 6G|FD-Score:-4.10|P-value:2.03E-5 ID:SGTC_121|Compound:0557-0495|FD-Score:-4.95|P-value:3.81E-7 ID:SGTC_135|Compound:0933-0004|FD-Score:6.81|P-value:7.60E-12 ID:SGTC_137|Compound:0438-0332|FD-Score:-4.78|P-value:8.75E-7 ID:SGTC_138|Compound:0850-0259|FD-Score:-3.82|P-value:6.59E-5 ID:SGTC_140|Compound:0062-0056|FD-Score:4.34|P-value:8.94E-6 ID:SGTC_141|Compound:0443-0274|FD-Score:-3.53|P-value:2.03E-4 ID:SGTC_213|Compound:1246-0773|FD-Score:4.58|P-value:2.91E-6 ID:SGTC_361|Compound:000s-0510|FD-Score:-3.29|P-value:4.88E-4 ID:SGTC_366|Compound:0181-0206|FD-Score:-3.22|P-value:6.21E-4 ID:SGTC_412|Compound:gemfibrozil|FD-Score:3.75|P-value:1.04E-4 ID:SGTC_527|Compound:4476-4215|FD-Score:7.66|P-value:1.50E-14 ID:SGTC_532|Compound:1548-0774|FD-Score:5.99|P-value:1.44E-9 ID:SGTC_534|Compound:k292-0788|FD-Score:4.09|P-value:2.60E-5 ID:SGTC_536|Compound:3852-0578|FD-Score:3.24|P-value:6.98E-4 ID:SGTC_541|Compound:4513-1321|FD-Score:4.56|P-value:3.23E-6 ID:SGTC_565|Compound:1418-0001|FD-Score:-4.02|P-value:2.96E-5 ID:SGTC_579|Compound:4491-1009|FD-Score:3.31|P-value:5.35E-4 ID:SGTC_865|Compound:0488-0499|FD-Score:-3.13|P-value:8.47E-4 ID:SGTC_869|Compound:0564-0080|FD-Score:3.67|P-value:1.43E-4 ID:SGTC_1024|Compound:4507-0395|FD-Score:3.54|P-value:2.34E-4 ID:SGTC_1027|Compound:k015-0025|FD-Score:5.24|P-value:1.04E-7 ID:SGTC_1035|Compound:k081-0041|FD-Score:-4.37|P-value:6.17E-6 ID:SGTC_1038|Compound:k292-0204|FD-Score:3.26|P-value:6.51E-4 ID:SGTC_1150|Compound:3909-7983|FD-Score:4.01|P-value:3.72E-5 ID:SGTC_1175|Compound:0741-0056|FD-Score:3.92|P-value:5.40E-5 ID:SGTC_1227|Compound:3909-8010|FD-Score:-3.12|P-value:8.92E-4 ID:SGTC_1247|Compound:0448-0043|FD-Score:3.16|P-value:9.10E-4 ID:SGTC_1264|Compound:0783-0040|FD-Score:3.77|P-value:9.73E-5 ID:SGTC_1584|Compound:6-fluoro-dl-tryptophan|FD-Score:-3.63|P-value:1.39E-4 ID:SGTC_1699|Compound:st031318|FD-Score:3.90|P-value:5.73E-5 ID:SGTC_1744|Compound:st037937|FD-Score:3.18|P-value:8.36E-4 ID:SGTC_1865|Compound:st057639|FD-Score:3.42|P-value:3.69E-4 ID:SGTC_1907|Compound:st060210|FD-Score:3.16|P-value:9.09E-4 ID:SGTC_1980|Compound:st071811|FD-Score:3.21|P-value:7.57E-4 ID:SGTC_2008|Compound:4012253|FD-Score:-3.60|P-value:1.58E-4 ID:SGTC_2019|Compound:4016004|FD-Score:5.44|P-value:3.63E-8 ID:SGTC_2136|Compound:5328288|FD-Score:-3.41|P-value:3.23E-4 ID:SGTC_2240|Compound:6690706|FD-Score:3.82|P-value:7.93E-5 ID:SGTC_2241|Compound:6772625|FD-Score:4.90|P-value:6.03E-7 ID:SGTC_2317|Compound:5986382|FD-Score:3.19|P-value:8.29E-4 ID:SGTC_2379|Compound:9035213|FD-Score:-3.13|P-value:8.54E-4 ID:SGTC_2401|Compound:5128683|FD-Score:-3.25|P-value:5.67E-4 ID:SGTC_2406|Compound:ethylparaben|FD-Score:-3.50|P-value:2.32E-4 ID:SGTC_2564|Compound:betulinic acid|FD-Score:5.21|P-value:1.26E-7 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:6.90|P-value:3.90E-12 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:5.03|P-value:3.09E-7 ID:SGTC_2634|Compound:4'-methoxyflavone|FD-Score:-3.66|P-value:1.24E-4 ID:SGTC_2640|Compound:pentamidine|FD-Score:-5.29|P-value:6.45E-8 ID:SGTC_2837|Compound:9008476|FD-Score:3.30|P-value:5.65E-4 ID:SGTC_2913|Compound:7966556|FD-Score:3.54|P-value:2.34E-4 ID:SGTC_2917|Compound:7979103|FD-Score:3.59|P-value:1.93E-4 ID:SGTC_2921|Compound:7974240|FD-Score:-3.22|P-value:6.26E-4 ID:SGTC_3059|Compound:1327-0034|FD-Score:3.15|P-value:9.42E-4 ID:SGTC_3341|Compound:9147222|FD-Score:3.65|P-value:1.54E-4

Top fitness defect scores for YBR114W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_527 4476-4215 7.66 268.0 µM 1.50E-14 PDR1 5 9.14E-15
2 SGTC_2631 bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone 6.90 100.0 µM 3.90E-12 7 2.56E-12
3 SGTC_135 0933-0004 6.81 26.4 µM 7.60E-12 60S ribosome export 1 5.04E-12
4 SGTC_532 1548-0774 5.99 215.0 µM 1.44E-9 1 1.03E-9
5 SGTC_2019 4016004 5.44 108.0 µM 3.63E-8 2 2.72E-8
6 SGTC_73 0327-0325 5.32 16.2 µM 6.93E-8 DNA damage response 20 5.24E-8
7 SGTC_1027 k015-0025 5.24 8.7 µM 1.04E-7 10 7.89E-8
8 SGTC_2564 betulinic acid 5.21 100.0 µM 1.26E-7 11 9.64E-8
9 SGTC_72 0744-0168 5.05 198.4 µM 2.87E-7 RPP1 & pyrimidine depletion 13 2.22E-7
10 SGTC_2632 3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone 5.03 100.0 µM 3.09E-7 9 2.39E-7
11 SGTC_2241 6772625 4.90 37.8 µM 6.03E-7 13 4.71E-7
12 SGTC_213 1246-0773 4.58 967.0 nM 2.91E-6 13 2.33E-6
13 SGTC_541 4513-1321 4.56 94.0 µM 3.23E-6 12 2.59E-6
14 SGTC_140 0062-0056 4.34 502.5 µM 8.94E-6 16 7.26E-6
15 SGTC_534 k292-0788 4.09 63.4 µM 2.60E-5 mitochondrial processes 26 2.15E-5
16 SGTC_1150 3909-7983 4.01 10.9 µM 3.72E-5 20 3.09E-5
17 SGTC_1175 0741-0056 3.92 27.6 µM 5.40E-5 17 4.51E-5
18 SGTC_1699 st031318 3.90 29.1 µM 5.73E-5 22 4.79E-5
19 SGTC_2240 6690706 3.82 185.9 µM 7.93E-5 20 6.66E-5
20 SGTC_1264 0783-0040 3.77 158.0 µM 9.73E-5 34 8.21E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.188 6.47E-28 YBR148W YSW1 hom Protein required for normal prospore membrane formation; interacts with Gip1p, which is the meiosis-specific regulatory subunit of the Glc7p protein phosphatase; expressed specifically in spores and localizes to the prospore membrane
0.115 2.63E-11 YGL042C_d YGL042C_d hom Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene DST1
0.097 1.60E-8 YDR045C RPC11 het RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS
0.093 7.22E-8 YNR027W BUD17 hom Putative pyridoxal kinase, a key enzyme in vitamin B6 metabolism; involved in bud-site selection; diploid mutants display a random rather than a bipolar budding pattern; similarity to yeast BUD16 and human pyridoxal kinase (PDXK)
0.092 1.10E-7 YKL116C PRR1 hom Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor
0.087 3.85E-7 YIL046W-A_p YIL046W-A_p hom Putative protein of unknown function; identified by expression profiling and mass spectrometry
0.087 4.08E-7 YDR147W EKI1 hom Ethanolamine kinase; primarily responsible for phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; exhibits some choline kinase activity, thus contributing to phosphatidylcholine synthesis via the CDP-choline pathway; EKI1 has a paralog, CKI1, that arose from the whole genome duplication
0.087 4.67E-7 YPR115W RGC1 hom Pleckstrin homology domain containing protein; proposed to function as a glycerol channel activator; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; RGC1 has a paralog, ASK10, that arose from the whole genome duplication
0.086 5.58E-7 YOR326W MYO2 het Type V myosin motor involved in actin-based transport of cargos; required for the polarized delivery of secretory vesicles, the vacuole, late Golgi elements, peroxisomes, and the mitotic spindle; MYO2 has a paralog, MYO4, that arose from the whole genome duplication
0.085 7.32E-7 YIL139C REV7 hom Accessory subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair
0.083 1.43E-6 YCL044C MGR1 hom Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr3p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA
0.081 2.28E-6 YDL240W LRG1 hom Putative GTPase-activating protein (GAP) involved in the Pkc1p-mediated signaling pathway that controls cell wall integrity; appears to specifically regulate 1,3-beta-glucan synthesis
0.080 3.27E-6 YIL145C PAN6 hom Pantothenate synthase, also known as pantoate-beta-alanine ligase, required for pantothenic acid biosynthesis, deletion causes pantothenic acid auxotrophy, homologous to E. coli panC
0.080 3.48E-6 YOL100W PKH2 hom Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p
0.079 4.01E-6 YCR088W ABP1 hom Actin-binding protein of the cortical actin cytoskeleton, important for activation of the Arp2/3 complex that plays a key role actin in cytoskeleton organization; phosphorylation within its PRR (Proline-Rich Region), mediated by Cdc28p and Pho85p, protects Abp1p from proteolysis mediated by its own PEST sequences