YBR121C / GRS1

Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication

Zygosity: Heterozygous strain
fixedexpanded
Profile for YBR121C / GRS1

Click on Significant Values for Screen Details ID:SGTC_353|Compound:1988-1175|FD-Score:-3.25|P-value:1.91E-4 ID:SGTC_412|Compound:gemfibrozil|FD-Score:-2.86|P-value:8.83E-4 ID:SGTC_432|Compound:NS 1619|FD-Score:-3.24|P-value:2.05E-4 ID:SGTC_587|Compound:k089-0003|FD-Score:3.42|P-value:1.18E-4 ID:SGTC_758|Compound:0868-0029|FD-Score:-5.68|P-value:3.34E-10 ID:SGTC_771|Compound:0250-0039|FD-Score:3.86|P-value:1.70E-5 ID:SGTC_835|Compound:0312-0010|FD-Score:3.26|P-value:2.30E-4 ID:SGTC_858|Compound:0438-0351|FD-Score:-3.28|P-value:1.69E-4 ID:SGTC_1021|Compound:4476-0760|FD-Score:-2.92|P-value:7.02E-4 ID:SGTC_1121|Compound:fusaric acid|FD-Score:3.00|P-value:6.46E-4 ID:SGTC_1189|Compound:1431-2119|FD-Score:3.34|P-value:1.66E-4 ID:SGTC_1264|Compound:0783-0040|FD-Score:-3.77|P-value:2.00E-5 ID:SGTC_1284|Compound:0929-0043|FD-Score:-3.01|P-value:5.14E-4 ID:SGTC_1288|Compound:0973-0013|FD-Score:3.00|P-value:6.40E-4 ID:SGTC_1291|Compound:0993-0025|FD-Score:-3.24|P-value:1.99E-4 ID:SGTC_1299|Compound:1079-1439|FD-Score:3.13|P-value:3.90E-4 ID:SGTC_1316|Compound:1272-0050|FD-Score:3.19|P-value:3.06E-4 ID:SGTC_1331|Compound:1472-0049|FD-Score:-2.90|P-value:7.75E-4 ID:SGTC_1336|Compound:1486-0901|FD-Score:3.91|P-value:1.32E-5 ID:SGTC_1350|Compound:1495-0454|FD-Score:4.25|P-value:2.41E-6 ID:SGTC_1372|Compound:2752-0194|FD-Score:-3.23|P-value:2.16E-4 ID:SGTC_1415|Compound:3966-0071|FD-Score:-3.14|P-value:3.08E-4 ID:SGTC_1488|Compound:4013-0813|FD-Score:-2.89|P-value:7.87E-4 ID:SGTC_1527|Compound:coumarin|FD-Score:3.81|P-value:2.12E-5 ID:SGTC_1628|Compound:st004252|FD-Score:3.10|P-value:4.43E-4 ID:SGTC_1634|Compound:st006576|FD-Score:-3.16|P-value:2.87E-4 ID:SGTC_1705|Compound:st027868|FD-Score:-2.84|P-value:9.72E-4 ID:SGTC_1733|Compound:st037357|FD-Score:3.04|P-value:5.52E-4 ID:SGTC_1737|Compound:st035557|FD-Score:-3.41|P-value:1.00E-4 ID:SGTC_1756|Compound:st041558|FD-Score:4.13|P-value:4.57E-6 ID:SGTC_1965|Compound:Pectolinarigenin|FD-Score:3.02|P-value:6.03E-4 ID:SGTC_1981|Compound:st071826|FD-Score:3.07|P-value:4.98E-4 ID:SGTC_2110|Compound:5483698|FD-Score:3.03|P-value:5.79E-4 ID:SGTC_2246|Compound:7293507|FD-Score:-4.20|P-value:2.36E-6 ID:SGTC_2316|Compound:5844804|FD-Score:3.73|P-value:3.09E-5 ID:SGTC_2436|Compound:5633522|FD-Score:3.10|P-value:4.39E-4 ID:SGTC_2455|Compound:5377712|FD-Score:-3.48|P-value:7.51E-5 ID:SGTC_2465|Compound:5809242|FD-Score:3.12|P-value:4.02E-4 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:3.17|P-value:3.36E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:-4.39|P-value:8.84E-7 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:3.77|P-value:2.53E-5 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:3.01|P-value:6.22E-4 ID:SGTC_2699|Compound:st077704|FD-Score:-3.34|P-value:1.34E-4 ID:SGTC_2701|Compound:st077767|FD-Score:-3.04|P-value:4.48E-4 ID:SGTC_2903|Compound:5923464|FD-Score:-3.39|P-value:1.10E-4 ID:SGTC_2965|Compound:9082772|FD-Score:-3.25|P-value:1.96E-4 ID:SGTC_3014|Compound:9081533|FD-Score:2.93|P-value:8.50E-4 ID:SGTC_3016|Compound:9080866|FD-Score:3.99|P-value:9.14E-6 ID:SGTC_3065|Compound:9113888|FD-Score:4.10|P-value:5.29E-6 ID:SGTC_3151|Compound:9099821|FD-Score:2.90|P-value:9.45E-4 ID:SGTC_3191|Compound:9110352|FD-Score:5.40|P-value:3.05E-9 ID:SGTC_3246|Compound:9135460|FD-Score:3.23|P-value:2.68E-4 ID:SGTC_353|Compound:1988-1175|FD-Score:-3.25|P-value:1.91E-4 ID:SGTC_412|Compound:gemfibrozil|FD-Score:-2.86|P-value:8.83E-4 ID:SGTC_432|Compound:NS 1619|FD-Score:-3.24|P-value:2.05E-4 ID:SGTC_587|Compound:k089-0003|FD-Score:3.42|P-value:1.18E-4 ID:SGTC_758|Compound:0868-0029|FD-Score:-5.68|P-value:3.34E-10 ID:SGTC_771|Compound:0250-0039|FD-Score:3.86|P-value:1.70E-5 ID:SGTC_835|Compound:0312-0010|FD-Score:3.26|P-value:2.30E-4 ID:SGTC_858|Compound:0438-0351|FD-Score:-3.28|P-value:1.69E-4 ID:SGTC_1021|Compound:4476-0760|FD-Score:-2.92|P-value:7.02E-4 ID:SGTC_1121|Compound:fusaric acid|FD-Score:3.00|P-value:6.46E-4 ID:SGTC_1189|Compound:1431-2119|FD-Score:3.34|P-value:1.66E-4 ID:SGTC_1264|Compound:0783-0040|FD-Score:-3.77|P-value:2.00E-5 ID:SGTC_1284|Compound:0929-0043|FD-Score:-3.01|P-value:5.14E-4 ID:SGTC_1288|Compound:0973-0013|FD-Score:3.00|P-value:6.40E-4 ID:SGTC_1291|Compound:0993-0025|FD-Score:-3.24|P-value:1.99E-4 ID:SGTC_1299|Compound:1079-1439|FD-Score:3.13|P-value:3.90E-4 ID:SGTC_1316|Compound:1272-0050|FD-Score:3.19|P-value:3.06E-4 ID:SGTC_1331|Compound:1472-0049|FD-Score:-2.90|P-value:7.75E-4 ID:SGTC_1336|Compound:1486-0901|FD-Score:3.91|P-value:1.32E-5 ID:SGTC_1350|Compound:1495-0454|FD-Score:4.25|P-value:2.41E-6 ID:SGTC_1372|Compound:2752-0194|FD-Score:-3.23|P-value:2.16E-4 ID:SGTC_1415|Compound:3966-0071|FD-Score:-3.14|P-value:3.08E-4 ID:SGTC_1488|Compound:4013-0813|FD-Score:-2.89|P-value:7.87E-4 ID:SGTC_1527|Compound:coumarin|FD-Score:3.81|P-value:2.12E-5 ID:SGTC_1628|Compound:st004252|FD-Score:3.10|P-value:4.43E-4 ID:SGTC_1634|Compound:st006576|FD-Score:-3.16|P-value:2.87E-4 ID:SGTC_1705|Compound:st027868|FD-Score:-2.84|P-value:9.72E-4 ID:SGTC_1733|Compound:st037357|FD-Score:3.04|P-value:5.52E-4 ID:SGTC_1737|Compound:st035557|FD-Score:-3.41|P-value:1.00E-4 ID:SGTC_1756|Compound:st041558|FD-Score:4.13|P-value:4.57E-6 ID:SGTC_1965|Compound:Pectolinarigenin|FD-Score:3.02|P-value:6.03E-4 ID:SGTC_1981|Compound:st071826|FD-Score:3.07|P-value:4.98E-4 ID:SGTC_2110|Compound:5483698|FD-Score:3.03|P-value:5.79E-4 ID:SGTC_2246|Compound:7293507|FD-Score:-4.20|P-value:2.36E-6 ID:SGTC_2316|Compound:5844804|FD-Score:3.73|P-value:3.09E-5 ID:SGTC_2436|Compound:5633522|FD-Score:3.10|P-value:4.39E-4 ID:SGTC_2455|Compound:5377712|FD-Score:-3.48|P-value:7.51E-5 ID:SGTC_2465|Compound:5809242|FD-Score:3.12|P-value:4.02E-4 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:3.17|P-value:3.36E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:-4.39|P-value:8.84E-7 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:3.77|P-value:2.53E-5 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:3.01|P-value:6.22E-4 ID:SGTC_2699|Compound:st077704|FD-Score:-3.34|P-value:1.34E-4 ID:SGTC_2701|Compound:st077767|FD-Score:-3.04|P-value:4.48E-4 ID:SGTC_2903|Compound:5923464|FD-Score:-3.39|P-value:1.10E-4 ID:SGTC_2965|Compound:9082772|FD-Score:-3.25|P-value:1.96E-4 ID:SGTC_3014|Compound:9081533|FD-Score:2.93|P-value:8.50E-4 ID:SGTC_3016|Compound:9080866|FD-Score:3.99|P-value:9.14E-6 ID:SGTC_3065|Compound:9113888|FD-Score:4.10|P-value:5.29E-6 ID:SGTC_3151|Compound:9099821|FD-Score:2.90|P-value:9.45E-4 ID:SGTC_3191|Compound:9110352|FD-Score:5.40|P-value:3.05E-9 ID:SGTC_3246|Compound:9135460|FD-Score:3.23|P-value:2.68E-4

Top fitness defect scores for YBR121C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_3191 9110352 5.40 49.5 µM 3.05E-9 1 3.37E-8 1.14
2 SGTC_1350 1495-0454 4.25 146.0 µM 2.41E-6 1 1.07E-5 0.44
3 SGTC_1756 st041558 4.13 35.5 µM 4.57E-6 3 1.85E-5 0.41
4 SGTC_3065 9113888 4.10 49.5 µM 5.29E-6 5 2.10E-5 0.23
5 SGTC_3016 9080866 3.99 49.5 µM 9.14E-6 3 3.37E-5 0.26
6 SGTC_1336 1486-0901 3.91 104.0 µM 1.32E-5 4 4.64E-5 0.66
7 SGTC_771 0250-0039 3.86 27.3 µM 1.70E-5 2 5.76E-5 0.17
8 SGTC_1527 coumarin 3.81 137.0 µM 2.12E-5 6 6.98E-5 0.00
9 SGTC_2566 crystal violet carbinol 3.77 226.0 nM 2.53E-5 22 8.15E-5 0.06
10 SGTC_2316 5844804 3.73 197.4 µM 3.09E-5 12 9.69E-5 0.16
11 SGTC_587 k089-0003 3.42 57.0 µM 1.18E-4 2 3.09E-4 0.02
12 SGTC_1189 1431-2119 3.34 7.3 µM 1.66E-4 15 4.15E-4 0.29
13 SGTC_835 0312-0010 3.26 67.2 µM 2.30E-4 3 5.51E-4 0.15
14 SGTC_3246 9135460 3.23 33.0 µM 2.68E-4 3 6.30E-4 0.13
15 SGTC_1316 1272-0050 3.19 65.9 µM 3.06E-4 5 7.06E-4 0.14
16 SGTC_2502 avocadyne acetate 3.17 18.6 µM 3.36E-4 11 7.67E-4 0.02
17 SGTC_1299 1079-1439 3.13 3.7 µM 3.90E-4 TSC3-RPN4 9 8.74E-4 0.02
18 SGTC_2465 5809242 3.12 33.2 µM 4.02E-4 6 8.96E-4 0.02
19 SGTC_2436 5633522 3.10 8.1 µM 4.39E-4 22 9.66E-4 0.06
20 SGTC_1628 st004252 3.10 77.7 µM 4.43E-4 12 9.75E-4 0.02

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.134 7.07E-15 YML025C YML6 het Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP-L4 protein; essential for viability, unlike most other mitoribosomal proteins
0.131 2.45E-14 YHL045W_d YHL045W_d hom Putative protein of unknown function; not an essential gene
0.130 3.66E-14 YIL009W FAA3 hom Long chain fatty acyl-CoA synthetase, activates imported fatty acids; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
0.128 1.15E-13 YML029W USA1 hom Scaffold subunit of the Hrd1p ubiquitin ligase that also promotes ligase oligomerization; involved in ER-associated protein degradation (ERAD); interacts with the U1 snRNP-specific protein, Snp1p
0.126 2.86E-13 YPL246C RBD2 hom Possible rhomboid protease, has similarity to eukaryotic rhomboid proteases including Pcp1p
0.123 7.80E-13 YOR271C_p FSF1_p hom Putative protein, predicted to be an alpha-isopropylmalate carrier; belongs to the sideroblastic-associated protein family; non-tagged protein is detected in purified mitochondria; likely to play a role in iron homeostasis
0.119 4.22E-12 YDL155W CLB3 hom B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; relative distribution to the nucleus increases upon DNA replication stress
0.112 8.38E-11 YDR242W AMD2 hom Putative amidase
0.104 1.66E-9 YGR274C TAF1 het TFIID subunit (145 kDa), involved in RNA polymerase II transcription initiation; possesses in vitro histone acetyltransferase activity but its role in vivo appears to be minor; involved in promoter binding and G1/S progression
0.103 2.07E-9 YOR218C_d YOR218C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene RFC1/YOR217W
0.099 1.04E-8 YGL114W_p YGL114W_p hom Putative protein of unknown function; predicted member of the oligopeptide transporter (OPT) family of membrane transporters
0.095 3.62E-8 YER053C-A YER053C-A hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress
0.094 5.21E-8 YLR040C_p YLR040C_p hom Protein of unknown function; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W; mRNA identified as translated by ribosome profiling data; YLR040C is not essential
0.093 6.58E-8 YPL113C_p YPL113C_p hom Glyoxylate reductase; acts on glyoxylate and hydroxypyruvate substrates; YPL113C is not an essential gene
0.091 1.47E-7 YBR084W MIS1 hom Mitochondrial C1-tetrahydrofolate synthase, involved in interconversion between different oxidation states of tetrahydrofolate (THF); provides activities of formyl-THF synthetase, methenyl-THF cyclohydrolase, and methylene-THF dehydrogenase