YBR131W / CCZ1

Protein involved in vacuolar assembly, essential for autophagy and the cytoplasm-to-vacuole pathway

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR131W / CCZ1

Click on Significant Values for Screen Details ID:SGTC_37|Compound:0566-0070|FD-Score:3.74|P-value:6.66E-5 ID:SGTC_44|Compound:0929-0055|FD-Score:-5.04|P-value:1.05E-7 ID:SGTC_65|Compound:0798-0905|FD-Score:8.21|P-value:2.08E-17 ID:SGTC_136|Compound:0097-0015|FD-Score:-3.09|P-value:7.10E-4 ID:SGTC_208|Compound:k072-0098|FD-Score:3.48|P-value:1.90E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:3.55|P-value:1.44E-4 ID:SGTC_245|Compound:methotrexate|FD-Score:4.71|P-value:7.34E-7 ID:SGTC_309|Compound:k408-0051|FD-Score:-3.33|P-value:2.96E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:3.18|P-value:5.70E-4 ID:SGTC_391|Compound:gliotoxin|FD-Score:11.80|P-value:3.06E-34 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:3.75|P-value:6.22E-5 ID:SGTC_528|Compound:3492-0019|FD-Score:10.20|P-value:4.74E-26 ID:SGTC_568|Compound:1193-0097|FD-Score:4.91|P-value:2.46E-7 ID:SGTC_661|Compound:1304-0007|FD-Score:3.06|P-value:8.90E-4 ID:SGTC_722|Compound:3534-0461|FD-Score:6.10|P-value:2.15E-10 ID:SGTC_805|Compound:3852-0218|FD-Score:6.40|P-value:2.80E-11 ID:SGTC_811|Compound:0416-0114|FD-Score:4.68|P-value:8.53E-7 ID:SGTC_824|Compound:1304-0008|FD-Score:3.63|P-value:1.05E-4 ID:SGTC_921|Compound:1561-0023|FD-Score:3.38|P-value:2.78E-4 ID:SGTC_1110|Compound:3-hydroxy-3'-methoxyflavone|FD-Score:4.88|P-value:3.00E-7 ID:SGTC_1133|Compound:3473-0247|FD-Score:3.16|P-value:6.14E-4 ID:SGTC_1217|Compound:1183-1422|FD-Score:3.83|P-value:4.52E-5 ID:SGTC_1413|Compound:3910-0535|FD-Score:3.76|P-value:5.94E-5 ID:SGTC_1456|Compound:k015-0012|FD-Score:3.10|P-value:7.81E-4 ID:SGTC_1457|Compound:k015-0032|FD-Score:3.06|P-value:8.81E-4 ID:SGTC_1520|Compound:flindersine|FD-Score:4.04|P-value:1.83E-5 ID:SGTC_1658|Compound:st012944|FD-Score:8.17|P-value:2.86E-17 ID:SGTC_1660|Compound:st012960|FD-Score:3.29|P-value:3.83E-4 ID:SGTC_1687|Compound:st020778|FD-Score:3.11|P-value:7.50E-4 ID:SGTC_1869|Compound:3',7'-dimethoxyflavone|FD-Score:-3.18|P-value:5.13E-4 ID:SGTC_1877|Compound:wortmannin|FD-Score:3.64|P-value:9.82E-5 ID:SGTC_1884|Compound:5633444|FD-Score:3.88|P-value:3.63E-5 ID:SGTC_1932|Compound:st074806|FD-Score:4.32|P-value:4.84E-6 ID:SGTC_1937|Compound:st074714|FD-Score:3.77|P-value:5.78E-5 ID:SGTC_1966|Compound:st070145|FD-Score:3.19|P-value:5.64E-4 ID:SGTC_2021|Compound:4019055|FD-Score:3.30|P-value:3.74E-4 ID:SGTC_2289|Compound:7946404|FD-Score:-3.16|P-value:5.69E-4 ID:SGTC_2306|Compound:7482397|FD-Score:-3.19|P-value:4.98E-4 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:4.31|P-value:5.16E-6 ID:SGTC_2443|Compound:5493264|FD-Score:3.98|P-value:2.33E-5 ID:SGTC_2481|Compound:5478948|FD-Score:9.03|P-value:1.06E-20 ID:SGTC_2586|Compound:curcumin|FD-Score:4.28|P-value:5.99E-6 ID:SGTC_2606|Compound:farnesol|FD-Score:10.50|P-value:2.29E-27 ID:SGTC_2613|Compound:3',4'-dimethoxydalbergione|FD-Score:4.07|P-value:1.54E-5 ID:SGTC_2701|Compound:st077767|FD-Score:3.15|P-value:6.55E-4 ID:SGTC_2702|Compound:st077768|FD-Score:3.27|P-value:4.10E-4 ID:SGTC_2737|Compound:bleomycin|FD-Score:3.22|P-value:5.03E-4 ID:SGTC_2743|Compound:auranofin|FD-Score:3.03|P-value:9.98E-4 ID:SGTC_2784|Compound:7567673|FD-Score:3.58|P-value:1.26E-4 ID:SGTC_2821|Compound:7996764|FD-Score:-4.11|P-value:1.13E-5 ID:SGTC_2931|Compound:9014674|FD-Score:3.39|P-value:2.66E-4 ID:SGTC_2948|Compound:9070189|FD-Score:4.12|P-value:1.24E-5 ID:SGTC_2958|Compound:9077254|FD-Score:3.42|P-value:2.33E-4 ID:SGTC_2967|Compound:9089538|FD-Score:3.22|P-value:5.04E-4 ID:SGTC_2970|Compound:9090938|FD-Score:5.56|P-value:6.19E-9 ID:SGTC_2971|Compound:9092186|FD-Score:6.71|P-value:3.12E-12 ID:SGTC_2972|Compound:9093619|FD-Score:-4.66|P-value:8.20E-7 ID:SGTC_2991|Compound:9074669|FD-Score:9.15|P-value:3.53E-21 ID:SGTC_2998|Compound:9066762|FD-Score:-4.93|P-value:1.92E-7 ID:SGTC_3061|Compound:glucosamine|FD-Score:3.39|P-value:2.67E-4 ID:SGTC_3081|Compound:9117243|FD-Score:3.58|P-value:1.26E-4 ID:SGTC_3193|Compound:9111931|FD-Score:3.28|P-value:3.98E-4 ID:SGTC_3200|Compound:9114385|FD-Score:3.23|P-value:4.86E-4 ID:SGTC_3351|Compound:9150312|FD-Score:-3.15|P-value:5.93E-4 ID:SGTC_37|Compound:0566-0070|FD-Score:3.74|P-value:6.66E-5 ID:SGTC_44|Compound:0929-0055|FD-Score:-5.04|P-value:1.05E-7 ID:SGTC_65|Compound:0798-0905|FD-Score:8.21|P-value:2.08E-17 ID:SGTC_136|Compound:0097-0015|FD-Score:-3.09|P-value:7.10E-4 ID:SGTC_208|Compound:k072-0098|FD-Score:3.48|P-value:1.90E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:3.55|P-value:1.44E-4 ID:SGTC_245|Compound:methotrexate|FD-Score:4.71|P-value:7.34E-7 ID:SGTC_309|Compound:k408-0051|FD-Score:-3.33|P-value:2.96E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:3.18|P-value:5.70E-4 ID:SGTC_391|Compound:gliotoxin|FD-Score:11.80|P-value:3.06E-34 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:3.75|P-value:6.22E-5 ID:SGTC_528|Compound:3492-0019|FD-Score:10.20|P-value:4.74E-26 ID:SGTC_568|Compound:1193-0097|FD-Score:4.91|P-value:2.46E-7 ID:SGTC_661|Compound:1304-0007|FD-Score:3.06|P-value:8.90E-4 ID:SGTC_722|Compound:3534-0461|FD-Score:6.10|P-value:2.15E-10 ID:SGTC_805|Compound:3852-0218|FD-Score:6.40|P-value:2.80E-11 ID:SGTC_811|Compound:0416-0114|FD-Score:4.68|P-value:8.53E-7 ID:SGTC_824|Compound:1304-0008|FD-Score:3.63|P-value:1.05E-4 ID:SGTC_921|Compound:1561-0023|FD-Score:3.38|P-value:2.78E-4 ID:SGTC_1110|Compound:3-hydroxy-3'-methoxyflavone|FD-Score:4.88|P-value:3.00E-7 ID:SGTC_1133|Compound:3473-0247|FD-Score:3.16|P-value:6.14E-4 ID:SGTC_1217|Compound:1183-1422|FD-Score:3.83|P-value:4.52E-5 ID:SGTC_1413|Compound:3910-0535|FD-Score:3.76|P-value:5.94E-5 ID:SGTC_1456|Compound:k015-0012|FD-Score:3.10|P-value:7.81E-4 ID:SGTC_1457|Compound:k015-0032|FD-Score:3.06|P-value:8.81E-4 ID:SGTC_1520|Compound:flindersine|FD-Score:4.04|P-value:1.83E-5 ID:SGTC_1658|Compound:st012944|FD-Score:8.17|P-value:2.86E-17 ID:SGTC_1660|Compound:st012960|FD-Score:3.29|P-value:3.83E-4 ID:SGTC_1687|Compound:st020778|FD-Score:3.11|P-value:7.50E-4 ID:SGTC_1869|Compound:3',7'-dimethoxyflavone|FD-Score:-3.18|P-value:5.13E-4 ID:SGTC_1877|Compound:wortmannin|FD-Score:3.64|P-value:9.82E-5 ID:SGTC_1884|Compound:5633444|FD-Score:3.88|P-value:3.63E-5 ID:SGTC_1932|Compound:st074806|FD-Score:4.32|P-value:4.84E-6 ID:SGTC_1937|Compound:st074714|FD-Score:3.77|P-value:5.78E-5 ID:SGTC_1966|Compound:st070145|FD-Score:3.19|P-value:5.64E-4 ID:SGTC_2021|Compound:4019055|FD-Score:3.30|P-value:3.74E-4 ID:SGTC_2289|Compound:7946404|FD-Score:-3.16|P-value:5.69E-4 ID:SGTC_2306|Compound:7482397|FD-Score:-3.19|P-value:4.98E-4 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:4.31|P-value:5.16E-6 ID:SGTC_2443|Compound:5493264|FD-Score:3.98|P-value:2.33E-5 ID:SGTC_2481|Compound:5478948|FD-Score:9.03|P-value:1.06E-20 ID:SGTC_2586|Compound:curcumin|FD-Score:4.28|P-value:5.99E-6 ID:SGTC_2606|Compound:farnesol|FD-Score:10.50|P-value:2.29E-27 ID:SGTC_2613|Compound:3',4'-dimethoxydalbergione|FD-Score:4.07|P-value:1.54E-5 ID:SGTC_2701|Compound:st077767|FD-Score:3.15|P-value:6.55E-4 ID:SGTC_2702|Compound:st077768|FD-Score:3.27|P-value:4.10E-4 ID:SGTC_2737|Compound:bleomycin|FD-Score:3.22|P-value:5.03E-4 ID:SGTC_2743|Compound:auranofin|FD-Score:3.03|P-value:9.98E-4 ID:SGTC_2784|Compound:7567673|FD-Score:3.58|P-value:1.26E-4 ID:SGTC_2821|Compound:7996764|FD-Score:-4.11|P-value:1.13E-5 ID:SGTC_2931|Compound:9014674|FD-Score:3.39|P-value:2.66E-4 ID:SGTC_2948|Compound:9070189|FD-Score:4.12|P-value:1.24E-5 ID:SGTC_2958|Compound:9077254|FD-Score:3.42|P-value:2.33E-4 ID:SGTC_2967|Compound:9089538|FD-Score:3.22|P-value:5.04E-4 ID:SGTC_2970|Compound:9090938|FD-Score:5.56|P-value:6.19E-9 ID:SGTC_2971|Compound:9092186|FD-Score:6.71|P-value:3.12E-12 ID:SGTC_2972|Compound:9093619|FD-Score:-4.66|P-value:8.20E-7 ID:SGTC_2991|Compound:9074669|FD-Score:9.15|P-value:3.53E-21 ID:SGTC_2998|Compound:9066762|FD-Score:-4.93|P-value:1.92E-7 ID:SGTC_3061|Compound:glucosamine|FD-Score:3.39|P-value:2.67E-4 ID:SGTC_3081|Compound:9117243|FD-Score:3.58|P-value:1.26E-4 ID:SGTC_3193|Compound:9111931|FD-Score:3.28|P-value:3.98E-4 ID:SGTC_3200|Compound:9114385|FD-Score:3.23|P-value:4.86E-4 ID:SGTC_3351|Compound:9150312|FD-Score:-3.15|P-value:5.93E-4

Top fitness defect scores for YBR131W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_391 gliotoxin 11.80 8.3 µM 3.06E-34 7 1.12E-32
2 SGTC_2606 farnesol 10.50 9.8 µM 2.29E-27 1 3.84E-26
3 SGTC_528 3492-0019 10.20 172.0 µM 4.74E-26 calcium & mitochondrial duress 38 6.86E-25
4 SGTC_2991 9074669 9.15 71.4 µM 3.53E-21 azole & statin 8 2.96E-20
5 SGTC_2481 5478948 9.03 45.2 µM 1.06E-20 PDR1 2 8.44E-20
6 SGTC_65 0798-0905 8.21 35.0 µM 2.08E-17 calcium & mitochondrial duress 15 1.14E-16
7 SGTC_1658 st012944 8.17 20.0 µM 2.86E-17 iron homeostasis 10 1.55E-16
8 SGTC_2971 9092186 6.71 9.9 µM 3.12E-12 ergosterol biosynthesis 4 9.63E-12
9 SGTC_805 3852-0218 6.40 479.0 µM 2.80E-11 ERAD & cell cycle 20 7.80E-11
10 SGTC_722 3534-0461 6.10 190.0 µM 2.15E-10 PDR1 6 5.43E-10
11 SGTC_2970 9090938 5.56 41.9 µM 6.19E-9 RNA processing & uracil transport 7 1.33E-8
12 SGTC_568 1193-0097 4.91 7.5 µM 2.46E-7 ERAD & cell cycle 89 4.46E-7
13 SGTC_1110 3-hydroxy-3'-methoxyflavone 4.88 1.2 µM 3.00E-7 iron homeostasis 27 5.39E-7
14 SGTC_245 methotrexate 4.71 289.6 µM 7.34E-7 25 1.27E-6
15 SGTC_811 0416-0114 4.68 265.0 µM 8.53E-7 7 1.46E-6
16 SGTC_1932 st074806 4.32 40.9 µM 4.84E-6 19 7.65E-6
17 SGTC_2410 aloe-emodin-8-o-glycoside 4.31 46.3 µM 5.16E-6 mitochondrial processes 21 8.12E-6
18 SGTC_2586 curcumin 4.28 80.0 µM 5.99E-6 iron homeostasis 39 9.37E-6
19 SGTC_2948 9070189 4.12 9.4 µM 1.24E-5 plasma membrane duress 41 1.88E-5
20 SGTC_2613 3',4'-dimethoxydalbergione 4.07 24.6 µM 1.54E-5 19 2.31E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.371 4.98E-110 YDR080W VPS41 hom Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport
0.317 4.22E-79 YGL212W VAM7 hom Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress
0.287 8.59E-65 YGL124C MON1 hom Protein required for fusion of cvt-vesicles and autophagosomes with the vacuole; associates, as a complex with Ccz1p, with a perivacuolar compartment; potential Cdc28p substrate
0.278 2.08E-60 YML001W YPT7 hom Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion; interacts with the cargo selection/retromer complex for retrograde sorting; similar to mammalian Rab7
0.234 5.72E-43 YBR288C APM3 hom Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway
0.232 4.64E-42 YDL077C VAM6 hom Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p
0.227 1.27E-40 YGR261C APL6 hom Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools
0.227 1.55E-40 YDR475C JIP4 hom Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence
0.175 1.75E-24 YPL195W APL5 hom Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function
0.175 2.26E-24 YDL192W ARF1 hom ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication
0.173 5.52E-24 YJL024C APS3 hom Small subunit of the clathrin-associated adaptor complex AP-3; involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; protein abundance increases in response to DNA replication stress
0.164 9.83E-22 YMR230W-A_p YMR230W-A_p hom Putative protein of unknown function
0.156 7.87E-20 YJR033C RAV1 hom Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate
0.156 9.04E-20 YOR086C TCB1 hom Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact
0.156 1.08E-19 YDR495C VPS3 hom Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase