YBR142W / MAK5

Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits

Zygosity: Heterozygous strain
fixedexpanded
Profile for YBR142W / MAK5

Click on Significant Values for Screen Details ID:SGTC_152|Compound:4469-0003|FD-Score:3.38|P-value:2.43E-4 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:4.49|P-value:1.75E-6 ID:SGTC_311|Compound:1315-0136|FD-Score:3.09|P-value:7.14E-4 ID:SGTC_367|Compound:0335-0881|FD-Score:8.06|P-value:4.21E-17 ID:SGTC_369|Compound:0957-0393|FD-Score:-4.53|P-value:1.44E-6 ID:SGTC_381|Compound:3461-2219|FD-Score:3.06|P-value:7.88E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:3.64|P-value:8.61E-5 ID:SGTC_549|Compound:1661-1316|FD-Score:3.36|P-value:2.60E-4 ID:SGTC_610|Compound:4215-0184|FD-Score:4.19|P-value:7.63E-6 ID:SGTC_684|Compound:1140-0066|FD-Score:3.20|P-value:4.84E-4 ID:SGTC_911|Compound:3456-4043|FD-Score:-3.74|P-value:5.61E-5 ID:SGTC_914|Compound:3820-6779|FD-Score:-6.07|P-value:1.86E-10 ID:SGTC_959|Compound:1222-0044|FD-Score:-3.59|P-value:1.05E-4 ID:SGTC_962|Compound:1254-0300|FD-Score:3.04|P-value:8.57E-4 ID:SGTC_1018|Compound:4259-0013|FD-Score:3.19|P-value:4.92E-4 ID:SGTC_1095|Compound:3-[3,5-dibromo-4-hydroxybenzoyl]-2-ethylbenzofuran|FD-Score:4.75|P-value:4.70E-7 ID:SGTC_1104|Compound:tacrolimus|FD-Score:4.02|P-value:1.63E-5 ID:SGTC_1120|Compound:ficusin|FD-Score:3.00|P-value:9.65E-4 ID:SGTC_1124|Compound:fisetin|FD-Score:-3.09|P-value:7.08E-4 ID:SGTC_1204|Compound:4049-0315|FD-Score:6.61|P-value:4.44E-12 ID:SGTC_1288|Compound:0973-0013|FD-Score:3.82|P-value:4.05E-5 ID:SGTC_1477|Compound:0139-0234|FD-Score:3.01|P-value:9.41E-4 ID:SGTC_1505|Compound:k297-0037|FD-Score:-3.31|P-value:3.13E-4 ID:SGTC_1520|Compound:flindersine|FD-Score:-3.50|P-value:1.49E-4 ID:SGTC_1579|Compound:chlormadinone acetate|FD-Score:-3.02|P-value:9.16E-4 ID:SGTC_1596|Compound:piperlongumine|FD-Score:5.52|P-value:5.95E-9 ID:SGTC_1601|Compound:4'-hydroxyflavanone|FD-Score:4.30|P-value:4.45E-6 ID:SGTC_1719|Compound:st032227|FD-Score:3.51|P-value:1.48E-4 ID:SGTC_1781|Compound:st038725|FD-Score:-3.64|P-value:8.55E-5 ID:SGTC_1862|Compound:st057513|FD-Score:-3.33|P-value:2.90E-4 ID:SGTC_1866|Compound:st057638|FD-Score:-3.02|P-value:9.07E-4 ID:SGTC_1873|Compound:rapamycin|FD-Score:3.44|P-value:1.92E-4 ID:SGTC_1917|Compound:st066507|FD-Score:3.39|P-value:2.30E-4 ID:SGTC_1953|Compound:st076800|FD-Score:4.22|P-value:6.74E-6 ID:SGTC_1954|Compound:st077032|FD-Score:-5.94|P-value:4.19E-10 ID:SGTC_2022|Compound:5136654|FD-Score:3.26|P-value:3.85E-4 ID:SGTC_2100|Compound:5377183|FD-Score:3.70|P-value:6.75E-5 ID:SGTC_2325|Compound:6158266|FD-Score:3.17|P-value:5.43E-4 ID:SGTC_2424|Compound:5537928|FD-Score:-3.91|P-value:2.74E-5 ID:SGTC_2568|Compound:diosmetin|FD-Score:7.25|P-value:3.65E-14 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:4.26|P-value:5.59E-6 ID:SGTC_2635|Compound:hydroquinidine|FD-Score:3.58|P-value:1.09E-4 ID:SGTC_2654|Compound:protoveratrine a|FD-Score:3.14|P-value:5.91E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:-3.62|P-value:9.42E-5 ID:SGTC_2695|Compound:st077686|FD-Score:3.43|P-value:2.00E-4 ID:SGTC_2722|Compound:naftopidil|FD-Score:3.03|P-value:8.66E-4 ID:SGTC_2749|Compound:bexarotene|FD-Score:3.60|P-value:1.03E-4 ID:SGTC_2754|Compound:granisetron|FD-Score:-3.17|P-value:5.25E-4 ID:SGTC_2772|Compound:vinpocetine|FD-Score:3.66|P-value:7.81E-5 ID:SGTC_2867|Compound:9039098|FD-Score:3.24|P-value:4.06E-4 ID:SGTC_2942|Compound:9052777|FD-Score:5.94|P-value:4.36E-10 ID:SGTC_2956|Compound:9076196|FD-Score:3.16|P-value:5.45E-4 ID:SGTC_2959|Compound:9079155|FD-Score:-3.14|P-value:5.89E-4 ID:SGTC_3080|Compound:9117754|FD-Score:3.12|P-value:6.47E-4 ID:SGTC_3098|Compound:9119039|FD-Score:3.40|P-value:2.26E-4 ID:SGTC_3175|Compound:9106517|FD-Score:3.06|P-value:7.98E-4 ID:SGTC_3225|Compound:9130819|FD-Score:4.78|P-value:4.08E-7 ID:SGTC_152|Compound:4469-0003|FD-Score:3.38|P-value:2.43E-4 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:4.49|P-value:1.75E-6 ID:SGTC_311|Compound:1315-0136|FD-Score:3.09|P-value:7.14E-4 ID:SGTC_367|Compound:0335-0881|FD-Score:8.06|P-value:4.21E-17 ID:SGTC_369|Compound:0957-0393|FD-Score:-4.53|P-value:1.44E-6 ID:SGTC_381|Compound:3461-2219|FD-Score:3.06|P-value:7.88E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:3.64|P-value:8.61E-5 ID:SGTC_549|Compound:1661-1316|FD-Score:3.36|P-value:2.60E-4 ID:SGTC_610|Compound:4215-0184|FD-Score:4.19|P-value:7.63E-6 ID:SGTC_684|Compound:1140-0066|FD-Score:3.20|P-value:4.84E-4 ID:SGTC_911|Compound:3456-4043|FD-Score:-3.74|P-value:5.61E-5 ID:SGTC_914|Compound:3820-6779|FD-Score:-6.07|P-value:1.86E-10 ID:SGTC_959|Compound:1222-0044|FD-Score:-3.59|P-value:1.05E-4 ID:SGTC_962|Compound:1254-0300|FD-Score:3.04|P-value:8.57E-4 ID:SGTC_1018|Compound:4259-0013|FD-Score:3.19|P-value:4.92E-4 ID:SGTC_1095|Compound:3-[3,5-dibromo-4-hydroxybenzoyl]-2-ethylbenzofuran|FD-Score:4.75|P-value:4.70E-7 ID:SGTC_1104|Compound:tacrolimus|FD-Score:4.02|P-value:1.63E-5 ID:SGTC_1120|Compound:ficusin|FD-Score:3.00|P-value:9.65E-4 ID:SGTC_1124|Compound:fisetin|FD-Score:-3.09|P-value:7.08E-4 ID:SGTC_1204|Compound:4049-0315|FD-Score:6.61|P-value:4.44E-12 ID:SGTC_1288|Compound:0973-0013|FD-Score:3.82|P-value:4.05E-5 ID:SGTC_1477|Compound:0139-0234|FD-Score:3.01|P-value:9.41E-4 ID:SGTC_1505|Compound:k297-0037|FD-Score:-3.31|P-value:3.13E-4 ID:SGTC_1520|Compound:flindersine|FD-Score:-3.50|P-value:1.49E-4 ID:SGTC_1579|Compound:chlormadinone acetate|FD-Score:-3.02|P-value:9.16E-4 ID:SGTC_1596|Compound:piperlongumine|FD-Score:5.52|P-value:5.95E-9 ID:SGTC_1601|Compound:4'-hydroxyflavanone|FD-Score:4.30|P-value:4.45E-6 ID:SGTC_1719|Compound:st032227|FD-Score:3.51|P-value:1.48E-4 ID:SGTC_1781|Compound:st038725|FD-Score:-3.64|P-value:8.55E-5 ID:SGTC_1862|Compound:st057513|FD-Score:-3.33|P-value:2.90E-4 ID:SGTC_1866|Compound:st057638|FD-Score:-3.02|P-value:9.07E-4 ID:SGTC_1873|Compound:rapamycin|FD-Score:3.44|P-value:1.92E-4 ID:SGTC_1917|Compound:st066507|FD-Score:3.39|P-value:2.30E-4 ID:SGTC_1953|Compound:st076800|FD-Score:4.22|P-value:6.74E-6 ID:SGTC_1954|Compound:st077032|FD-Score:-5.94|P-value:4.19E-10 ID:SGTC_2022|Compound:5136654|FD-Score:3.26|P-value:3.85E-4 ID:SGTC_2100|Compound:5377183|FD-Score:3.70|P-value:6.75E-5 ID:SGTC_2325|Compound:6158266|FD-Score:3.17|P-value:5.43E-4 ID:SGTC_2424|Compound:5537928|FD-Score:-3.91|P-value:2.74E-5 ID:SGTC_2568|Compound:diosmetin|FD-Score:7.25|P-value:3.65E-14 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:4.26|P-value:5.59E-6 ID:SGTC_2635|Compound:hydroquinidine|FD-Score:3.58|P-value:1.09E-4 ID:SGTC_2654|Compound:protoveratrine a|FD-Score:3.14|P-value:5.91E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:-3.62|P-value:9.42E-5 ID:SGTC_2695|Compound:st077686|FD-Score:3.43|P-value:2.00E-4 ID:SGTC_2722|Compound:naftopidil|FD-Score:3.03|P-value:8.66E-4 ID:SGTC_2749|Compound:bexarotene|FD-Score:3.60|P-value:1.03E-4 ID:SGTC_2754|Compound:granisetron|FD-Score:-3.17|P-value:5.25E-4 ID:SGTC_2772|Compound:vinpocetine|FD-Score:3.66|P-value:7.81E-5 ID:SGTC_2867|Compound:9039098|FD-Score:3.24|P-value:4.06E-4 ID:SGTC_2942|Compound:9052777|FD-Score:5.94|P-value:4.36E-10 ID:SGTC_2956|Compound:9076196|FD-Score:3.16|P-value:5.45E-4 ID:SGTC_2959|Compound:9079155|FD-Score:-3.14|P-value:5.89E-4 ID:SGTC_3080|Compound:9117754|FD-Score:3.12|P-value:6.47E-4 ID:SGTC_3098|Compound:9119039|FD-Score:3.40|P-value:2.26E-4 ID:SGTC_3175|Compound:9106517|FD-Score:3.06|P-value:7.98E-4 ID:SGTC_3225|Compound:9130819|FD-Score:4.78|P-value:4.08E-7

Top fitness defect scores for YBR142W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_367 0335-0881 8.06 7.1 µM 4.21E-17 ubiquinone biosynthesis & proteasome 6 3.69E-16 0.03
2 SGTC_2568 diosmetin 7.25 100.0 µM 3.65E-14 2 2.12E-13 2.63
3 SGTC_1204 4049-0315 6.61 18.3 µM 4.44E-12 2 1.93E-11 1.13
4 SGTC_2942 9052777 5.94 62.2 µM 4.36E-10 2 1.43E-9 0.91
5 SGTC_1596 piperlongumine 5.52 63.0 µM 5.95E-9 1 1.67E-8 1.25
6 SGTC_3225 9130819 4.78 49.5 µM 4.08E-7 amide catabolism 4 8.89E-7 0.08
7 SGTC_1095 3-[3,5-dibromo-4-hydroxybenzoyl]-2-ethylbenzofuran 4.75 15.5 µM 4.70E-7 1 1.02E-6 0.63
8 SGTC_242 5hr heat shock (37°C) + 5-fluorouracil 4.49 4.6 µM 1.75E-6 heat shock/prefoldin 19 3.50E-6 0.72
9 SGTC_1601 4'-hydroxyflavanone 4.30 55.5 µM 4.45E-6 RPP1 & pyrimidine depletion 3 8.42E-6 0.19
10 SGTC_2572 astragaloside iv 4.26 100.0 µM 5.59E-6 4 1.04E-5 0.20
11 SGTC_1953 st076800 4.22 12.1 µM 6.74E-6 13 1.24E-5 0.00
12 SGTC_610 4215-0184 4.19 155.0 µM 7.63E-6 exosome 22 1.40E-5 0.13
13 SGTC_1104 tacrolimus 4.02 29.8 µM 1.63E-5 7 2.86E-5 0.06
14 SGTC_1288 0973-0013 3.82 65.5 µM 4.05E-5 2 6.72E-5 0.39
15 SGTC_2100 5377183 3.70 190.0 µM 6.75E-5 60S ribosome export 9 1.09E-4 0.02
16 SGTC_2772 vinpocetine 3.66 57.1 µM 7.81E-5 7 1.25E-4 0.09
17 SGTC_423 5-fluorouracil 3.64 457.0 µM 8.61E-5 exosome 34 1.37E-4 0.03
18 SGTC_2749 bexarotene 3.60 57.4 µM 1.03E-4 9 1.61E-4 0.20
19 SGTC_2635 hydroquinidine 3.58 100.0 µM 1.09E-4 3 1.71E-4 0.51
20 SGTC_1719 st032227 3.51 49.2 µM 1.48E-4 14 2.28E-4 0.16

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.171 2.26E-23 YPL044C_d YPL044C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W
0.141 1.89E-16 YGR195W SKI6 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4)
0.137 1.67E-15 YPL043W NOP4 het Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs)
0.125 4.29E-13 YDR059C UBC5 hom Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived, abnormal, or excess proteins, including histone H3; central component of the cellular stress response; expression is heat inducible; protein abundance increases in response to DNA replication stress; UBC5 has a paralog, UBC4, that arose from the whole genome duplication
0.122 1.63E-12 YKL172W EBP2 het Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering
0.119 5.06E-12 YDR118W APC4 het Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to the nucleus increases upon DNA replication stress
0.112 8.66E-11 YDR464W SPP41 het Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3
0.109 2.51E-10 YGR158C MTR3 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6)
0.106 7.55E-10 YNL089C_d YNL089C_d hom Dubious open reading frame unlikely to encode a functional protein; almost completely overlaps YNL090W/RHO2 which encodes a small GTPase of the Rho/Rac subfamily of Ras-like proteins
0.098 1.11E-8 YDL111C RRP42 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7)
0.096 2.45E-8 YGR079W_p YGR079W_p hom Putative protein of unknown function; YGR079W is not an essential gene
0.095 3.66E-8 YOL021C DIS3 het Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress
0.091 1.40E-7 YLR029C RPL15A het Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication
0.089 2.66E-7 YBL100W-C_p YBL100W-C_p hom Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
0.089 2.79E-7 YJR152W DAL5 hom Allantoate permease; ureidosuccinate permease; also transports dipeptides, though with lower affinity than for allantoate and ureidosuccinate; expression is constitutive but sensitive to nitrogen catabolite repression