HIPHOP chemogenomics database
YBR143C / SUP45 
Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p
Zygosity: Heterozygous strain
fixedexpanded
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Top fitness defect scores for YBR143C deletion by condition
Download Fitness data (tab-delimited text) (excel) |
Cofit Genes
Download Cofitness data (tab-delimited text) (excel)| Correlation | pval | ORF | Gene | Zygosity | Description |
|---|---|---|---|---|---|
| 0.341 | 6.50E-92 | YPR104C | FHL1 | het | Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants |
| 0.327 | 1.27E-84 | YBR091C | TIM12 | het | Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins |
| 0.326 | 6.59E-84 | YPR107C | YTH1 | het | Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation |
| 0.303 | 3.80E-72 | YOR236W | DFR1 | het | Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism |
| 0.301 | 2.30E-71 | YLL043W | FPS1 | hom | Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress |
| 0.295 | 2.06E-68 | YLR321C | SFH1 | het | Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog |
| 0.282 | 2.41E-62 | YDR172W | SUP35 | het | Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype |
| 0.281 | 4.03E-62 | YLR331C_d | JIP3_d | hom | Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2 |
| 0.271 | 1.65E-57 | YIL126W | STH1 | het | ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p |
| 0.264 | 2.27E-54 | YLR033W | RSC58 | het | Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance |
| 0.256 | 3.55E-51 | YGL093W | SPC105 | het | Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components |
| 0.253 | 4.84E-50 | YJL081C | ARP4 | het | Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes |
| 0.251 | 3.17E-49 | YJL039C | NUP192 | het | Essential subunit of the inner ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport; homologous to human NUP205 |
| 0.245 | 6.09E-47 | YNR016C | ACC1 | het | Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; |
| 0.245 | 6.42E-47 | YER165W | PAB1 | het | Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G |