YBR143C / SUP45

Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p

Zygosity: Heterozygous strain
fixedexpanded
Profile for YBR143C / SUP45

Click on Significant Values for Screen Details ID:SGTC_22|Compound:0964-0034|FD-Score:4.06|P-value:4.13E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:8.66|P-value:2.05E-17 ID:SGTC_63|Compound:0099-0242|FD-Score:3.69|P-value:1.75E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:12.00|P-value:8.93E-32 ID:SGTC_103|Compound:0844-0104|FD-Score:4.13|P-value:3.12E-5 ID:SGTC_129|Compound:0088-0017|FD-Score:-4.72|P-value:2.16E-6 ID:SGTC_138|Compound:0850-0259|FD-Score:9.11|P-value:4.38E-19 ID:SGTC_157|Compound:1348-1695|FD-Score:5.27|P-value:1.53E-7 ID:SGTC_168|Compound:3970-0862|FD-Score:3.78|P-value:1.24E-4 ID:SGTC_248|Compound:streptozotocin|FD-Score:4.33|P-value:1.35E-5 ID:SGTC_268|Compound:camptothecin|FD-Score:12.10|P-value:3.38E-32 ID:SGTC_332|Compound:4112-0506|FD-Score:3.82|P-value:1.04E-4 ID:SGTC_354|Compound:2783-3500|FD-Score:3.79|P-value:1.19E-4 ID:SGTC_449|Compound:xestospongin c|FD-Score:3.58|P-value:2.61E-4 ID:SGTC_451|Compound:chloral hydrate|FD-Score:4.25|P-value:1.84E-5 ID:SGTC_546|Compound:peri-Naphthalide|FD-Score:3.97|P-value:5.91E-5 ID:SGTC_564|Compound:3555-0129|FD-Score:-3.31|P-value:6.31E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:-4.34|P-value:1.17E-5 ID:SGTC_569|Compound:4130-2812|FD-Score:3.79|P-value:1.19E-4 ID:SGTC_572|Compound:0960-0087|FD-Score:6.68|P-value:4.36E-11 ID:SGTC_576|Compound:1073-0113|FD-Score:4.02|P-value:4.73E-5 ID:SGTC_580|Compound:1123-0028|FD-Score:6.65|P-value:5.49E-11 ID:SGTC_586|Compound:0957-0265|FD-Score:16.90|P-value:7.08E-61 ID:SGTC_628|Compound:1636-0253|FD-Score:4.51|P-value:6.14E-6 ID:SGTC_637|Compound:1181-0519|FD-Score:-3.41|P-value:4.38E-4 ID:SGTC_641|Compound:4092-0595|FD-Score:5.96|P-value:3.68E-9 ID:SGTC_642|Compound:0335-0876|FD-Score:3.58|P-value:2.61E-4 ID:SGTC_666|Compound:0272-0154|FD-Score:3.63|P-value:2.15E-4 ID:SGTC_673|Compound:1082-0474|FD-Score:10.20|P-value:1.36E-23 ID:SGTC_674|Compound:0498-0004|FD-Score:5.72|P-value:1.44E-8 ID:SGTC_794|Compound:k038-0031|FD-Score:5.02|P-value:5.49E-7 ID:SGTC_814|Compound:3342-0569|FD-Score:3.45|P-value:4.08E-4 ID:SGTC_885|Compound:0865-0106|FD-Score:3.42|P-value:4.58E-4 ID:SGTC_916|Compound:4466-0038|FD-Score:3.22|P-value:8.92E-4 ID:SGTC_943|Compound:3253-1379|FD-Score:-3.29|P-value:6.74E-4 ID:SGTC_948|Compound:3448-0295|FD-Score:3.93|P-value:6.82E-5 ID:SGTC_1074|Compound:carmofur|FD-Score:3.32|P-value:6.34E-4 ID:SGTC_1374|Compound:2889-5571|FD-Score:5.31|P-value:1.27E-7 ID:SGTC_1493|Compound:4348-0038|FD-Score:6.15|P-value:1.21E-9 ID:SGTC_1530|Compound:rhein|FD-Score:4.07|P-value:3.99E-5 ID:SGTC_1536|Compound:cytidine-2',3'-monophosphoric acid;  3'-cytidylic acid|FD-Score:4.93|P-value:8.50E-7 ID:SGTC_1537|Compound:2',6'-dihydroxyflavanone|FD-Score:3.90|P-value:7.86E-5 ID:SGTC_1540|Compound:guanosine|FD-Score:3.55|P-value:2.89E-4 ID:SGTC_1560|Compound:lithocholic acid|FD-Score:-3.43|P-value:4.16E-4 ID:SGTC_1564|Compound:D-Arginine|FD-Score:-3.72|P-value:1.45E-4 ID:SGTC_1584|Compound:6-fluoro-dl-tryptophan|FD-Score:3.23|P-value:8.77E-4 ID:SGTC_1646|Compound:st011709|FD-Score:3.27|P-value:7.54E-4 ID:SGTC_1652|Compound:st011932|FD-Score:6.18|P-value:9.90E-10 ID:SGTC_1817|Compound:st052116|FD-Score:-4.11|P-value:3.19E-5 ID:SGTC_2041|Compound:5107442|FD-Score:3.42|P-value:4.49E-4 ID:SGTC_2046|Compound:4110841|FD-Score:3.30|P-value:6.81E-4 ID:SGTC_2050|Compound:5263056|FD-Score:3.37|P-value:5.46E-4 ID:SGTC_2129|Compound:girgensonine|FD-Score:5.21|P-value:2.17E-7 ID:SGTC_2250|Compound:alverine citrate|FD-Score:-3.31|P-value:6.27E-4 ID:SGTC_2289|Compound:7946404|FD-Score:5.01|P-value:5.94E-7 ID:SGTC_2411|Compound:st077133|FD-Score:-4.93|P-value:7.78E-7 ID:SGTC_2417|Compound:cerulenin|FD-Score:5.01|P-value:5.91E-7 ID:SGTC_2530|Compound:3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene|FD-Score:3.38|P-value:5.23E-4 ID:SGTC_2581|Compound:avocatin a|FD-Score:-3.43|P-value:4.09E-4 ID:SGTC_2589|Compound:lobaric acid|FD-Score:4.87|P-value:1.14E-6 ID:SGTC_2727|Compound:riluzole|FD-Score:3.37|P-value:5.35E-4 ID:SGTC_2728|Compound:chlorpromazine|FD-Score:4.24|P-value:1.94E-5 ID:SGTC_2759|Compound:disulfiram|FD-Score:3.65|P-value:1.98E-4 ID:SGTC_2792|Compound:7535235|FD-Score:6.12|P-value:1.42E-9 ID:SGTC_2799|Compound:7812892|FD-Score:4.07|P-value:3.93E-5 ID:SGTC_2848|Compound:9016370|FD-Score:-3.46|P-value:3.70E-4 ID:SGTC_2874|Compound:9043904|FD-Score:-8.13|P-value:1.28E-15 ID:SGTC_2878|Compound:9043973|FD-Score:-6.60|P-value:6.44E-11 ID:SGTC_2912|Compound:7964298|FD-Score:4.82|P-value:1.45E-6 ID:SGTC_2921|Compound:7974240|FD-Score:-5.73|P-value:1.19E-8 ID:SGTC_2925|Compound:9010536|FD-Score:3.26|P-value:7.99E-4 ID:SGTC_2928|Compound:9011484|FD-Score:5.90|P-value:4.98E-9 ID:SGTC_2951|Compound:9072950|FD-Score:3.74|P-value:1.43E-4 ID:SGTC_2957|Compound:9076800|FD-Score:3.80|P-value:1.13E-4 ID:SGTC_3138|Compound:9097216|FD-Score:3.34|P-value:5.92E-4 ID:SGTC_3145|Compound:9085516|FD-Score:3.61|P-value:2.29E-4 ID:SGTC_3188|Compound:9110789|FD-Score:3.33|P-value:6.28E-4 ID:SGTC_3200|Compound:9114385|FD-Score:-3.33|P-value:5.95E-4 ID:SGTC_22|Compound:0964-0034|FD-Score:4.06|P-value:4.13E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:8.66|P-value:2.05E-17 ID:SGTC_63|Compound:0099-0242|FD-Score:3.69|P-value:1.75E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:12.00|P-value:8.93E-32 ID:SGTC_103|Compound:0844-0104|FD-Score:4.13|P-value:3.12E-5 ID:SGTC_129|Compound:0088-0017|FD-Score:-4.72|P-value:2.16E-6 ID:SGTC_138|Compound:0850-0259|FD-Score:9.11|P-value:4.38E-19 ID:SGTC_157|Compound:1348-1695|FD-Score:5.27|P-value:1.53E-7 ID:SGTC_168|Compound:3970-0862|FD-Score:3.78|P-value:1.24E-4 ID:SGTC_248|Compound:streptozotocin|FD-Score:4.33|P-value:1.35E-5 ID:SGTC_268|Compound:camptothecin|FD-Score:12.10|P-value:3.38E-32 ID:SGTC_332|Compound:4112-0506|FD-Score:3.82|P-value:1.04E-4 ID:SGTC_354|Compound:2783-3500|FD-Score:3.79|P-value:1.19E-4 ID:SGTC_449|Compound:xestospongin c|FD-Score:3.58|P-value:2.61E-4 ID:SGTC_451|Compound:chloral hydrate|FD-Score:4.25|P-value:1.84E-5 ID:SGTC_546|Compound:peri-Naphthalide|FD-Score:3.97|P-value:5.91E-5 ID:SGTC_564|Compound:3555-0129|FD-Score:-3.31|P-value:6.31E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:-4.34|P-value:1.17E-5 ID:SGTC_569|Compound:4130-2812|FD-Score:3.79|P-value:1.19E-4 ID:SGTC_572|Compound:0960-0087|FD-Score:6.68|P-value:4.36E-11 ID:SGTC_576|Compound:1073-0113|FD-Score:4.02|P-value:4.73E-5 ID:SGTC_580|Compound:1123-0028|FD-Score:6.65|P-value:5.49E-11 ID:SGTC_586|Compound:0957-0265|FD-Score:16.90|P-value:7.08E-61 ID:SGTC_628|Compound:1636-0253|FD-Score:4.51|P-value:6.14E-6 ID:SGTC_637|Compound:1181-0519|FD-Score:-3.41|P-value:4.38E-4 ID:SGTC_641|Compound:4092-0595|FD-Score:5.96|P-value:3.68E-9 ID:SGTC_642|Compound:0335-0876|FD-Score:3.58|P-value:2.61E-4 ID:SGTC_666|Compound:0272-0154|FD-Score:3.63|P-value:2.15E-4 ID:SGTC_673|Compound:1082-0474|FD-Score:10.20|P-value:1.36E-23 ID:SGTC_674|Compound:0498-0004|FD-Score:5.72|P-value:1.44E-8 ID:SGTC_794|Compound:k038-0031|FD-Score:5.02|P-value:5.49E-7 ID:SGTC_814|Compound:3342-0569|FD-Score:3.45|P-value:4.08E-4 ID:SGTC_885|Compound:0865-0106|FD-Score:3.42|P-value:4.58E-4 ID:SGTC_916|Compound:4466-0038|FD-Score:3.22|P-value:8.92E-4 ID:SGTC_943|Compound:3253-1379|FD-Score:-3.29|P-value:6.74E-4 ID:SGTC_948|Compound:3448-0295|FD-Score:3.93|P-value:6.82E-5 ID:SGTC_1074|Compound:carmofur|FD-Score:3.32|P-value:6.34E-4 ID:SGTC_1374|Compound:2889-5571|FD-Score:5.31|P-value:1.27E-7 ID:SGTC_1493|Compound:4348-0038|FD-Score:6.15|P-value:1.21E-9 ID:SGTC_1530|Compound:rhein|FD-Score:4.07|P-value:3.99E-5 ID:SGTC_1536|Compound:cytidine-2',3'-monophosphoric acid;  3'-cytidylic acid|FD-Score:4.93|P-value:8.50E-7 ID:SGTC_1537|Compound:2',6'-dihydroxyflavanone|FD-Score:3.90|P-value:7.86E-5 ID:SGTC_1540|Compound:guanosine|FD-Score:3.55|P-value:2.89E-4 ID:SGTC_1560|Compound:lithocholic acid|FD-Score:-3.43|P-value:4.16E-4 ID:SGTC_1564|Compound:D-Arginine|FD-Score:-3.72|P-value:1.45E-4 ID:SGTC_1584|Compound:6-fluoro-dl-tryptophan|FD-Score:3.23|P-value:8.77E-4 ID:SGTC_1646|Compound:st011709|FD-Score:3.27|P-value:7.54E-4 ID:SGTC_1652|Compound:st011932|FD-Score:6.18|P-value:9.90E-10 ID:SGTC_1817|Compound:st052116|FD-Score:-4.11|P-value:3.19E-5 ID:SGTC_2041|Compound:5107442|FD-Score:3.42|P-value:4.49E-4 ID:SGTC_2046|Compound:4110841|FD-Score:3.30|P-value:6.81E-4 ID:SGTC_2050|Compound:5263056|FD-Score:3.37|P-value:5.46E-4 ID:SGTC_2129|Compound:girgensonine|FD-Score:5.21|P-value:2.17E-7 ID:SGTC_2250|Compound:alverine citrate|FD-Score:-3.31|P-value:6.27E-4 ID:SGTC_2289|Compound:7946404|FD-Score:5.01|P-value:5.94E-7 ID:SGTC_2411|Compound:st077133|FD-Score:-4.93|P-value:7.78E-7 ID:SGTC_2417|Compound:cerulenin|FD-Score:5.01|P-value:5.91E-7 ID:SGTC_2530|Compound:3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene|FD-Score:3.38|P-value:5.23E-4 ID:SGTC_2581|Compound:avocatin a|FD-Score:-3.43|P-value:4.09E-4 ID:SGTC_2589|Compound:lobaric acid|FD-Score:4.87|P-value:1.14E-6 ID:SGTC_2727|Compound:riluzole|FD-Score:3.37|P-value:5.35E-4 ID:SGTC_2728|Compound:chlorpromazine|FD-Score:4.24|P-value:1.94E-5 ID:SGTC_2759|Compound:disulfiram|FD-Score:3.65|P-value:1.98E-4 ID:SGTC_2792|Compound:7535235|FD-Score:6.12|P-value:1.42E-9 ID:SGTC_2799|Compound:7812892|FD-Score:4.07|P-value:3.93E-5 ID:SGTC_2848|Compound:9016370|FD-Score:-3.46|P-value:3.70E-4 ID:SGTC_2874|Compound:9043904|FD-Score:-8.13|P-value:1.28E-15 ID:SGTC_2878|Compound:9043973|FD-Score:-6.60|P-value:6.44E-11 ID:SGTC_2912|Compound:7964298|FD-Score:4.82|P-value:1.45E-6 ID:SGTC_2921|Compound:7974240|FD-Score:-5.73|P-value:1.19E-8 ID:SGTC_2925|Compound:9010536|FD-Score:3.26|P-value:7.99E-4 ID:SGTC_2928|Compound:9011484|FD-Score:5.90|P-value:4.98E-9 ID:SGTC_2951|Compound:9072950|FD-Score:3.74|P-value:1.43E-4 ID:SGTC_2957|Compound:9076800|FD-Score:3.80|P-value:1.13E-4 ID:SGTC_3138|Compound:9097216|FD-Score:3.34|P-value:5.92E-4 ID:SGTC_3145|Compound:9085516|FD-Score:3.61|P-value:2.29E-4 ID:SGTC_3188|Compound:9110789|FD-Score:3.33|P-value:6.28E-4 ID:SGTC_3200|Compound:9114385|FD-Score:-3.33|P-value:5.95E-4

Top fitness defect scores for YBR143C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_586 0957-0265 16.90 8.2 µM 7.08E-61 cell wall 3 2.34E-64 6.58
2 SGTC_268 camptothecin 12.10 39.6 µM 3.38E-32 DNA damage response 4 5.33E-34 1.91
3 SGTC_64 000l-0018 12.00 272.1 µM 8.93E-32 ubiquinone biosynthesis & proteasome 10 1.49E-33 2.27
4 SGTC_673 1082-0474 10.20 110.0 µM 1.36E-23 RSC complex & mRNA processing 10 6.82E-25 0.28
5 SGTC_138 0850-0259 9.11 40.9 µM 4.38E-19 cell wall 14 4.03E-20 0.36
6 SGTC_58 0957-0393 8.66 9.0 µM 2.05E-17 cell wall 10 2.36E-18 0.24
7 SGTC_572 0960-0087 6.68 17.7 µM 4.36E-11 2 1.18E-11 0.83
8 SGTC_580 1123-0028 6.65 41.0 µM 5.49E-11 DNA damage response 4 1.50E-11 0.19
9 SGTC_1652 st011932 6.18 78.0 µM 9.90E-10 RSC complex & mRNA processing 25 3.21E-10 0.34
10 SGTC_1493 4348-0038 6.15 111.0 µM 1.21E-9 RSC complex & mRNA processing 15 3.98E-10 0.04
11 SGTC_2792 7535235 6.12 81.8 µM 1.42E-9 fatty acid desaturase (OLE1) 3 4.70E-10 0.77
12 SGTC_641 4092-0595 5.96 30.0 µM 3.68E-9 3 1.29E-9 1.31
13 SGTC_2928 9011484 5.90 9.8 µM 4.98E-9 heme biosynthesis & mitochondrial translocase 11 1.77E-9 0.26
14 SGTC_674 0498-0004 5.72 451.0 µM 1.44E-8 heme requiring 6 5.47E-9 0.45
15 SGTC_1374 2889-5571 5.31 141.0 µM 1.27E-7 RSC complex & mRNA processing 25 5.47E-8 0.16
16 SGTC_157 1348-1695 5.27 65.8 µM 1.53E-7 iron homeostasis 22 6.65E-8 0.14
17 SGTC_2129 girgensonine 5.21 121.0 µM 2.17E-7 heme biosynthesis & mitochondrial translocase 13 9.63E-8 0.21
18 SGTC_794 k038-0031 5.02 316.0 µM 5.49E-7 ubiquinone biosynthesis & proteasome 11 2.56E-7 0.17
19 SGTC_2417 cerulenin 5.01 830.0 nM 5.91E-7 7 2.77E-7 0.22
20 SGTC_2289 7946404 5.01 165.1 µM 5.94E-7 12 2.79E-7 0.23

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.341 6.50E-92 YPR104C FHL1 het Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants
0.327 1.27E-84 YBR091C TIM12 het Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins
0.326 6.59E-84 YPR107C YTH1 het Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation
0.303 3.80E-72 YOR236W DFR1 het Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism
0.301 2.30E-71 YLL043W FPS1 hom Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress
0.295 2.06E-68 YLR321C SFH1 het Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog
0.282 2.41E-62 YDR172W SUP35 het Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype
0.281 4.03E-62 YLR331C_d JIP3_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
0.271 1.65E-57 YIL126W STH1 het ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p
0.264 2.27E-54 YLR033W RSC58 het Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
0.256 3.55E-51 YGL093W SPC105 het Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components
0.253 4.84E-50 YJL081C ARP4 het Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes
0.251 3.17E-49 YJL039C NUP192 het Essential subunit of the inner ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport; homologous to human NUP205
0.245 6.09E-47 YNR016C ACC1 het Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids;
0.245 6.42E-47 YER165W PAB1 het Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G