YBR154C / RPB5

RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation

Zygosity: Heterozygous strain
fixedexpanded
Profile for YBR154C / RPB5

Click on Significant Values for Screen Details ID:SGTC_35|Compound:0133-0054|FD-Score:4.02|P-value:6.39E-5 ID:SGTC_91|Compound:0417-1751|FD-Score:4.20|P-value:3.20E-5 ID:SGTC_152|Compound:4469-0003|FD-Score:5.64|P-value:4.84E-8 ID:SGTC_168|Compound:3970-0862|FD-Score:5.80|P-value:2.13E-8 ID:SGTC_221|Compound:trifluoperazine|FD-Score:7.87|P-value:6.43E-14 ID:SGTC_255|Compound:tunicamycin|FD-Score:-3.56|P-value:6.35E-4 ID:SGTC_301|Compound:4119-0100|FD-Score:3.65|P-value:2.48E-4 ID:SGTC_308|Compound:4092-1169|FD-Score:3.46|P-value:4.70E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:3.30|P-value:7.88E-4 ID:SGTC_362|Compound:0310-0100|FD-Score:3.31|P-value:7.65E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:-5.35|P-value:5.06E-7 ID:SGTC_445|Compound:e6 berbamine|FD-Score:6.05|P-value:5.33E-9 ID:SGTC_562|Compound:3772-2733|FD-Score:3.38|P-value:6.04E-4 ID:SGTC_576|Compound:1073-0113|FD-Score:4.06|P-value:5.63E-5 ID:SGTC_583|Compound:1488-1091|FD-Score:3.35|P-value:6.66E-4 ID:SGTC_671|Compound:0560-0003|FD-Score:3.27|P-value:8.66E-4 ID:SGTC_674|Compound:0498-0004|FD-Score:-5.56|P-value:1.87E-7 ID:SGTC_720|Compound:r052-1644|FD-Score:-3.59|P-value:5.86E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:3.82|P-value:1.34E-4 ID:SGTC_829|Compound:1635-0120|FD-Score:8.05|P-value:1.80E-14 ID:SGTC_871|Compound:0574-0004|FD-Score:4.87|P-value:1.94E-6 ID:SGTC_1001|Compound:4048-4355|FD-Score:3.44|P-value:5.06E-4 ID:SGTC_1031|Compound:k035-0009|FD-Score:5.86|P-value:1.51E-8 ID:SGTC_1044|Compound:1222-0021|FD-Score:-4.35|P-value:3.76E-5 ID:SGTC_1058|Compound:3448-5425|FD-Score:5.76|P-value:2.59E-8 ID:SGTC_1151|Compound:3909-8316|FD-Score:5.54|P-value:8.07E-8 ID:SGTC_1323|Compound:1348-1351|FD-Score:5.34|P-value:2.17E-7 ID:SGTC_1327|Compound:1436-0082|FD-Score:4.03|P-value:6.32E-5 ID:SGTC_1465|Compound:k213-0056|FD-Score:3.61|P-value:2.80E-4 ID:SGTC_1490|Compound:4089-0339|FD-Score:3.55|P-value:3.46E-4 ID:SGTC_1700|Compound:st030840|FD-Score:3.40|P-value:5.69E-4 ID:SGTC_1739|Compound:st037196|FD-Score:3.28|P-value:8.44E-4 ID:SGTC_1754|Compound:st042065|FD-Score:3.58|P-value:3.17E-4 ID:SGTC_1798|Compound:st045719|FD-Score:3.29|P-value:8.24E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:4.81|P-value:2.56E-6 ID:SGTC_1855|Compound:st056195|FD-Score:-3.63|P-value:5.03E-4 ID:SGTC_1915|Compound:st067105|FD-Score:9.54|P-value:1.57E-19 ID:SGTC_1930|Compound:st074634|FD-Score:6.35|P-value:9.96E-10 ID:SGTC_1991|Compound:st073000|FD-Score:5.01|P-value:1.02E-6 ID:SGTC_2041|Compound:5107442|FD-Score:5.45|P-value:1.23E-7 ID:SGTC_2109|Compound:5483026|FD-Score:4.85|P-value:2.13E-6 ID:SGTC_2126|Compound:5251174|FD-Score:6.69|P-value:1.42E-10 ID:SGTC_2147|Compound:5545932|FD-Score:4.76|P-value:3.15E-6 ID:SGTC_2150|Compound:5556613|FD-Score:3.84|P-value:1.27E-4 ID:SGTC_2161|Compound:5662504|FD-Score:3.43|P-value:5.14E-4 ID:SGTC_2186|Compound:6124449|FD-Score:3.94|P-value:8.78E-5 ID:SGTC_2250|Compound:alverine citrate|FD-Score:4.78|P-value:2.92E-6 ID:SGTC_2331|Compound:7980721|FD-Score:4.11|P-value:4.66E-5 ID:SGTC_2378|Compound:9074213|FD-Score:3.37|P-value:6.36E-4 ID:SGTC_2437|Compound:5741531|FD-Score:-4.44|P-value:2.69E-5 ID:SGTC_2544|Compound:sphondin|FD-Score:14.80|P-value:4.54E-44 ID:SGTC_2549|Compound:cedrol|FD-Score:3.58|P-value:3.16E-4 ID:SGTC_2561|Compound:acetosyringone|FD-Score:3.62|P-value:2.75E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:-3.73|P-value:3.61E-4 ID:SGTC_2585|Compound:alliin|FD-Score:3.57|P-value:3.29E-4 ID:SGTC_2612|Compound:dictamnine|FD-Score:3.92|P-value:9.54E-5 ID:SGTC_2640|Compound:pentamidine|FD-Score:-3.57|P-value:6.13E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:5.03|P-value:9.41E-7 ID:SGTC_2766|Compound:oltipraz|FD-Score:-3.54|P-value:6.79E-4 ID:SGTC_2776|Compound:terbinafine|FD-Score:4.09|P-value:4.89E-5 ID:SGTC_2903|Compound:5923464|FD-Score:4.55|P-value:7.75E-6 ID:SGTC_2909|Compound:7941780|FD-Score:3.59|P-value:3.02E-4 ID:SGTC_3037|Compound:9091026|FD-Score:3.95|P-value:8.39E-5 ID:SGTC_3105|Compound:9121040|FD-Score:-3.62|P-value:5.27E-4 ID:SGTC_3126|Compound:9127922|FD-Score:-6.13|P-value:1.03E-8 ID:SGTC_3152|Compound:9099253|FD-Score:4.61|P-value:6.01E-6 ID:SGTC_3208|Compound:9114115|FD-Score:-3.45|P-value:9.02E-4 ID:SGTC_3212|Compound:9128307|FD-Score:4.03|P-value:6.32E-5 ID:SGTC_3247|Compound:9135149|FD-Score:8.88|P-value:3.50E-17 ID:SGTC_3282|Compound:9108498|FD-Score:3.65|P-value:2.51E-4 ID:SGTC_35|Compound:0133-0054|FD-Score:4.02|P-value:6.39E-5 ID:SGTC_91|Compound:0417-1751|FD-Score:4.20|P-value:3.20E-5 ID:SGTC_152|Compound:4469-0003|FD-Score:5.64|P-value:4.84E-8 ID:SGTC_168|Compound:3970-0862|FD-Score:5.80|P-value:2.13E-8 ID:SGTC_221|Compound:trifluoperazine|FD-Score:7.87|P-value:6.43E-14 ID:SGTC_255|Compound:tunicamycin|FD-Score:-3.56|P-value:6.35E-4 ID:SGTC_301|Compound:4119-0100|FD-Score:3.65|P-value:2.48E-4 ID:SGTC_308|Compound:4092-1169|FD-Score:3.46|P-value:4.70E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:3.30|P-value:7.88E-4 ID:SGTC_362|Compound:0310-0100|FD-Score:3.31|P-value:7.65E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:-5.35|P-value:5.06E-7 ID:SGTC_445|Compound:e6 berbamine|FD-Score:6.05|P-value:5.33E-9 ID:SGTC_562|Compound:3772-2733|FD-Score:3.38|P-value:6.04E-4 ID:SGTC_576|Compound:1073-0113|FD-Score:4.06|P-value:5.63E-5 ID:SGTC_583|Compound:1488-1091|FD-Score:3.35|P-value:6.66E-4 ID:SGTC_671|Compound:0560-0003|FD-Score:3.27|P-value:8.66E-4 ID:SGTC_674|Compound:0498-0004|FD-Score:-5.56|P-value:1.87E-7 ID:SGTC_720|Compound:r052-1644|FD-Score:-3.59|P-value:5.86E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:3.82|P-value:1.34E-4 ID:SGTC_829|Compound:1635-0120|FD-Score:8.05|P-value:1.80E-14 ID:SGTC_871|Compound:0574-0004|FD-Score:4.87|P-value:1.94E-6 ID:SGTC_1001|Compound:4048-4355|FD-Score:3.44|P-value:5.06E-4 ID:SGTC_1031|Compound:k035-0009|FD-Score:5.86|P-value:1.51E-8 ID:SGTC_1044|Compound:1222-0021|FD-Score:-4.35|P-value:3.76E-5 ID:SGTC_1058|Compound:3448-5425|FD-Score:5.76|P-value:2.59E-8 ID:SGTC_1151|Compound:3909-8316|FD-Score:5.54|P-value:8.07E-8 ID:SGTC_1323|Compound:1348-1351|FD-Score:5.34|P-value:2.17E-7 ID:SGTC_1327|Compound:1436-0082|FD-Score:4.03|P-value:6.32E-5 ID:SGTC_1465|Compound:k213-0056|FD-Score:3.61|P-value:2.80E-4 ID:SGTC_1490|Compound:4089-0339|FD-Score:3.55|P-value:3.46E-4 ID:SGTC_1700|Compound:st030840|FD-Score:3.40|P-value:5.69E-4 ID:SGTC_1739|Compound:st037196|FD-Score:3.28|P-value:8.44E-4 ID:SGTC_1754|Compound:st042065|FD-Score:3.58|P-value:3.17E-4 ID:SGTC_1798|Compound:st045719|FD-Score:3.29|P-value:8.24E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:4.81|P-value:2.56E-6 ID:SGTC_1855|Compound:st056195|FD-Score:-3.63|P-value:5.03E-4 ID:SGTC_1915|Compound:st067105|FD-Score:9.54|P-value:1.57E-19 ID:SGTC_1930|Compound:st074634|FD-Score:6.35|P-value:9.96E-10 ID:SGTC_1991|Compound:st073000|FD-Score:5.01|P-value:1.02E-6 ID:SGTC_2041|Compound:5107442|FD-Score:5.45|P-value:1.23E-7 ID:SGTC_2109|Compound:5483026|FD-Score:4.85|P-value:2.13E-6 ID:SGTC_2126|Compound:5251174|FD-Score:6.69|P-value:1.42E-10 ID:SGTC_2147|Compound:5545932|FD-Score:4.76|P-value:3.15E-6 ID:SGTC_2150|Compound:5556613|FD-Score:3.84|P-value:1.27E-4 ID:SGTC_2161|Compound:5662504|FD-Score:3.43|P-value:5.14E-4 ID:SGTC_2186|Compound:6124449|FD-Score:3.94|P-value:8.78E-5 ID:SGTC_2250|Compound:alverine citrate|FD-Score:4.78|P-value:2.92E-6 ID:SGTC_2331|Compound:7980721|FD-Score:4.11|P-value:4.66E-5 ID:SGTC_2378|Compound:9074213|FD-Score:3.37|P-value:6.36E-4 ID:SGTC_2437|Compound:5741531|FD-Score:-4.44|P-value:2.69E-5 ID:SGTC_2544|Compound:sphondin|FD-Score:14.80|P-value:4.54E-44 ID:SGTC_2549|Compound:cedrol|FD-Score:3.58|P-value:3.16E-4 ID:SGTC_2561|Compound:acetosyringone|FD-Score:3.62|P-value:2.75E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:-3.73|P-value:3.61E-4 ID:SGTC_2585|Compound:alliin|FD-Score:3.57|P-value:3.29E-4 ID:SGTC_2612|Compound:dictamnine|FD-Score:3.92|P-value:9.54E-5 ID:SGTC_2640|Compound:pentamidine|FD-Score:-3.57|P-value:6.13E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:5.03|P-value:9.41E-7 ID:SGTC_2766|Compound:oltipraz|FD-Score:-3.54|P-value:6.79E-4 ID:SGTC_2776|Compound:terbinafine|FD-Score:4.09|P-value:4.89E-5 ID:SGTC_2903|Compound:5923464|FD-Score:4.55|P-value:7.75E-6 ID:SGTC_2909|Compound:7941780|FD-Score:3.59|P-value:3.02E-4 ID:SGTC_3037|Compound:9091026|FD-Score:3.95|P-value:8.39E-5 ID:SGTC_3105|Compound:9121040|FD-Score:-3.62|P-value:5.27E-4 ID:SGTC_3126|Compound:9127922|FD-Score:-6.13|P-value:1.03E-8 ID:SGTC_3152|Compound:9099253|FD-Score:4.61|P-value:6.01E-6 ID:SGTC_3208|Compound:9114115|FD-Score:-3.45|P-value:9.02E-4 ID:SGTC_3212|Compound:9128307|FD-Score:4.03|P-value:6.32E-5 ID:SGTC_3247|Compound:9135149|FD-Score:8.88|P-value:3.50E-17 ID:SGTC_3282|Compound:9108498|FD-Score:3.65|P-value:2.51E-4

Top fitness defect scores for YBR154C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2544 sphondin 14.80 68.5 µM 4.54E-44 RNA pol III & RNase P/MRP 3 5.15E-50 1.94
2 SGTC_1915 st067105 9.54 30.6 µM 1.57E-19 RNA pol III & RNase P/MRP 8 6.94E-22 1.13
3 SGTC_3247 9135149 8.88 49.5 µM 3.50E-17 DNA damage response 3 3.35E-19 0.33
4 SGTC_829 1635-0120 8.05 117.0 µM 1.80E-14 DNA damage response 14 4.15E-16 0.01
5 SGTC_221 trifluoperazine 7.87 10.3 µM 6.43E-14 NEO1-PIK1 10 1.78E-15 1.17
6 SGTC_2126 5251174 6.69 33.9 µM 1.42E-10 2 1.14E-11 0.93
7 SGTC_1930 st074634 6.35 34.3 µM 9.96E-10 RNA pol III & RNase P/MRP 8 1.05E-10 0.81
8 SGTC_445 e6 berbamine 6.05 66.0 µM 5.33E-9 NEO1-PIK1 16 7.03E-10 0.29
9 SGTC_1031 k035-0009 5.86 16.0 µM 1.51E-8 2 2.29E-9 2.28
10 SGTC_168 3970-0862 5.80 530.5 µM 2.13E-8 18 3.39E-9 0.01
11 SGTC_1058 3448-5425 5.76 97.3 µM 2.59E-8 1 4.22E-9 0.54
12 SGTC_152 4469-0003 5.64 551.2 µM 4.84E-8 3 8.59E-9 0.01
13 SGTC_1151 3909-8316 5.54 15.3 µM 8.07E-8 2 1.53E-8 0.60
14 SGTC_2041 5107442 5.45 45.3 µM 1.23E-7 RSC complex & mRNA processing 19 2.47E-8 0.12
15 SGTC_1323 1348-1351 5.34 11.2 µM 2.17E-7 1 4.70E-8 0.96
16 SGTC_2679 rapamycin 5.03 1.0 nM 9.41E-7 35 2.47E-7 0.02
17 SGTC_1991 st073000 5.01 8.9 µM 1.02E-6 NEO1 9 2.70E-7 0.01
18 SGTC_871 0574-0004 4.87 149.0 µM 1.94E-6 19 5.59E-7 0.19
19 SGTC_2109 5483026 4.85 200.0 µM 2.13E-6 RSC complex & mRNA processing 27 6.23E-7 0.19
20 SGTC_1844 rapamycin 4.81 4.0 nM 2.56E-6 35 7.65E-7 0.03

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.365 3.62E-106 YOR116C RPO31 het RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
0.246 2.81E-47 YFL002C SPB4 het Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients
0.239 1.11E-44 YNL248C RPA49 hom RNA polymerase I subunit A49
0.234 7.99E-43 YER165W PAB1 het Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G
0.233 1.69E-42 YDR225W HTA1 hom Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p
0.232 5.77E-42 YOR207C RET1 het Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
0.231 1.08E-41 YJL081C ARP4 het Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes
0.227 2.83E-40 YBR091C TIM12 het Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins
0.221 2.62E-38 YBL026W LSM2 het Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
0.214 8.18E-36 YJL154C VPS35 hom Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p
0.209 2.65E-34 YDL150W RPC53 het RNA polymerase III subunit C53
0.204 7.87E-33 YBL092W RPL32 het Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog
0.202 3.75E-32 YOR191W ULS1 hom Protein involved in proteolytic control of sumoylated substrates; contains RING finger domain; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.198 5.93E-31 YHR012W VPS29 hom Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval
0.196 2.28E-30 YFL023W BUD27 hom Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP