HIPHOP chemogenomics database

YBR161W / CSH1

Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p; CSH1 has a paralog, SUR1, that arose from the whole genome duplication

Zygosity: Homozygous strain

fixedexpanded
Profile for YBR161W / CSH1

Click on Significant Values for Screen Details

Top fitness defect scores for YBR161W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_2995	9067767	6.29	71.4 µM	2.90E-12		4	1.62E-10
2	SGTC_2097	5376186	5.75	200.0 µM	1.55E-10		5	4.58E-9
3	SGTC_3201	9113999	5.05	49.5 µM	1.54E-8		13	2.18E-7
4	SGTC_2339	9000433	5.01	200.0 µM	2.01E-8		12	2.72E-7
5	SGTC_3219	9131112	4.56	49.5 µM	2.95E-7	Golgi	28	2.61E-6
6	SGTC_1239	0337-0584	4.41	35.4 µM	6.82E-7	unfolded protein response	43	5.27E-6
7	SGTC_3224	9130643	4.23	49.5 µM	1.77E-6	RPP1 & pyrimidine depletion	19	1.18E-5
8	SGTC_2102	5377816	4.11	72.8 µM	3.30E-6		24	1.98E-5
9	SGTC_1145	3546-0597	4.09	10.2 µM	3.62E-6	mitochondrial processes	18	2.14E-5
10	SGTC_1342	1486-1464	3.97	234.0 µM	6.80E-6		16	3.65E-5
11	SGTC_163	1327-0036	3.88	7.5 µM	1.02E-5		109	5.14E-5
12	SGTC_1179	1021-1431	3.84	9.3 µM	1.29E-5		45	6.27E-5
13	SGTC_2916	7875993	3.69	68.8 µM	2.60E-5		37	1.13E-4
14	SGTC_1146	3579-1023	3.68	7.0 µM	2.72E-5		22	1.17E-4
15	SGTC_3346	9158254	3.65	43.6 µM	3.04E-5		27	1.29E-4
16	SGTC_2182	6325192	3.62	200.0 µM	3.58E-5	Golgi	20	1.48E-4
17	SGTC_2712	st079126	3.57	47.7 µM	4.45E-5		26	1.78E-4
18	SGTC_2919	7978188	3.52	46.4 µM	5.58E-5		36	2.15E-4
19	SGTC_1860	st056228	3.48	74.6 µM	6.77E-5		33	2.53E-4
20	SGTC_3055	9094636	3.42	49.5 µM	8.92E-5		20	3.19E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.151	1.81E-18	YPL116W	HOS3	hom	Trichostatin A-insensitive homodimeric histone deacetylase (HDAC) with specificity in vitro for histones H3, H4, H2A, and H2B; similar to Hda1p, Rpd3p, Hos1p, and Hos2p; deletion results in increased histone acetylation at rDNA repeats
0.140	3.77E-16	YML130C	ERO1	het	Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds
0.136	3.25E-15	YKR031C	SPO14	hom	Phospholipase D; catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions
0.123	8.65E-13	YFL032W_d	YFL032W_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene
0.110	1.92E-10	YPR126C_d	YPR126C_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.102	3.92E-9	YDR221W	GTB1	hom	Glucosidase II beta subunit, forms a complex with alpha subunit Rot2p; involved in removal of two glucose residues from N-linked glycans during glycoprotein biogenesis in the ER; relocalizes from ER to cytoplasm upon DNA replication stress
0.100	7.45E-9	YIR008C	PRI1	het	Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair
0.096	2.21E-8	YOR026W	BUB3	hom	Kinetochore checkpoint WD40 repeat protein that localizes to kinetochores during prophase and metaphase, delays anaphase in the presence of unattached kinetochores; forms complexes with Mad1p-Bub1p and with Cdc20p, binds Mad2p and Mad3p
0.096	2.79E-8	YGR193C	PDX1	hom	Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) of the mitochondrial pyruvate dehydrogenase (PDH) complex, plays a structural role in the complex by binding and positioning E3 to the dihydrolipoamide acetyltransferase (E2) core
0.096	2.85E-8	YOR135C_d	IRC14_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci
0.093	7.22E-8	YKL067W	YNK1	hom	Nucleoside diphosphate kinase; catalyzes the transfer of gamma phosphates from nucleoside triphosphates, usually ATP, to nucleoside diphosphates by a mechanism that involves formation of an autophosphorylated enzyme intermediate; protein abundance increases in response to DNA replication stress
0.092	9.79E-8	YBR031W	RPL4A	hom	Ribosomal 60S subunit protein L4A; N-terminally acetylated; homologous to mammalian ribosomal protein L4 and bacterial L4; RPL4A has a paralog, RPL4B, that arose from the whole genome duplication
0.092	1.05E-7	YHR105W	YPT35	hom	Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport
0.090	1.53E-7	YHL016C	DUR3	hom	Plasma membrane transporter for both urea and polyamines, expression is highly sensitive to nitrogen catabolite repression and induced by allophanate, the last intermediate of the allantoin degradative pathway
0.086	5.43E-7	YLR147C	SMD3	het	Core Sm protein Sm D3; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D3