YBR162C / TOS1

Covalently-bound cell wall protein of unknown function; identified as a cell cycle regulated SBF target gene; deletion mutants are highly resistant to treatment with beta-1,3-glucanase; has sequence similarity to YJL171C

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR162C / TOS1

Click on Significant Values for Screen Details ID:SGTC_36|Compound:0335-0881|FD-Score:-3.66|P-value:3.63E-4 ID:SGTC_86|Compound:0417-1630|FD-Score:-3.80|P-value:2.20E-4 ID:SGTC_121|Compound:0557-0495|FD-Score:-4.15|P-value:5.92E-5 ID:SGTC_255|Compound:tunicamycin|FD-Score:6.42|P-value:2.58E-10 ID:SGTC_260|Compound:nsc-65238|FD-Score:-3.40|P-value:8.50E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:-3.76|P-value:2.49E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:-3.75|P-value:2.57E-4 ID:SGTC_408|Compound:cantharidin|FD-Score:4.58|P-value:4.15E-6 ID:SGTC_528|Compound:3492-0019|FD-Score:-4.02|P-value:9.79E-5 ID:SGTC_617|Compound:3770-0098|FD-Score:3.30|P-value:5.96E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:5.97|P-value:3.76E-9 ID:SGTC_641|Compound:4092-0595|FD-Score:3.24|P-value:7.29E-4 ID:SGTC_660|Compound:4031-1742|FD-Score:3.32|P-value:5.61E-4 ID:SGTC_791|Compound:1348-1292|FD-Score:3.62|P-value:1.92E-4 ID:SGTC_794|Compound:k038-0031|FD-Score:-3.94|P-value:1.32E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:7.04|P-value:5.13E-12 ID:SGTC_914|Compound:3820-6779|FD-Score:-4.23|P-value:4.32E-5 ID:SGTC_991|Compound:3996-0057|FD-Score:3.54|P-value:2.60E-4 ID:SGTC_1234|Compound:0326-0494|FD-Score:-3.84|P-value:1.90E-4 ID:SGTC_1325|Compound:1431-0015|FD-Score:-3.67|P-value:3.46E-4 ID:SGTC_1326|Compound:1431-2094|FD-Score:-3.51|P-value:5.88E-4 ID:SGTC_1341|Compound:1486-1360|FD-Score:-6.15|P-value:4.31E-9 ID:SGTC_1348|Compound:1493-0373|FD-Score:3.36|P-value:4.80E-4 ID:SGTC_1450|Compound:4112-4260|FD-Score:3.76|P-value:1.17E-4 ID:SGTC_1489|Compound:4065-0444|FD-Score:3.63|P-value:1.91E-4 ID:SGTC_1517|Compound:deoxycholic acid methyl ester|FD-Score:3.31|P-value:5.83E-4 ID:SGTC_1521|Compound:st012942|FD-Score:4.15|P-value:2.53E-5 ID:SGTC_1529|Compound:apigenin|FD-Score:3.24|P-value:7.28E-4 ID:SGTC_1635|Compound:st007035|FD-Score:4.02|P-value:4.32E-5 ID:SGTC_1712|Compound:st032288|FD-Score:-4.40|P-value:2.20E-5 ID:SGTC_1789|Compound:tunicamycin|FD-Score:3.20|P-value:8.24E-4 ID:SGTC_1818|Compound:st052136|FD-Score:-3.82|P-value:2.05E-4 ID:SGTC_1891|Compound:st059073|FD-Score:3.75|P-value:1.22E-4 ID:SGTC_2300|Compound:7976697|FD-Score:-3.39|P-value:8.81E-4 ID:SGTC_2309|Compound:7664088|FD-Score:3.39|P-value:4.35E-4 ID:SGTC_2318|Compound:6029776|FD-Score:4.78|P-value:1.66E-6 ID:SGTC_2442|Compound:5158906|FD-Score:-4.35|P-value:2.71E-5 ID:SGTC_2489|Compound:cantharidin|FD-Score:4.53|P-value:5.05E-6 ID:SGTC_2662|Compound:daunorubicin|FD-Score:3.43|P-value:3.87E-4 ID:SGTC_2689|Compound:tacrolimus|FD-Score:4.19|P-value:2.13E-5 ID:SGTC_2694|Compound:st077685|FD-Score:-3.60|P-value:4.45E-4 ID:SGTC_2780|Compound:5215298|FD-Score:4.32|P-value:1.29E-5 ID:SGTC_2798|Compound:7813637|FD-Score:3.85|P-value:8.48E-5 ID:SGTC_2962|Compound:9082311|FD-Score:-3.54|P-value:5.46E-4 ID:SGTC_2964|Compound:9083345|FD-Score:3.79|P-value:1.04E-4 ID:SGTC_2975|Compound:9084847|FD-Score:4.65|P-value:2.94E-6 ID:SGTC_2986|Compound:9022445|FD-Score:4.47|P-value:6.61E-6 ID:SGTC_3002|Compound:9075603|FD-Score:3.94|P-value:5.83E-5 ID:SGTC_3006|Compound:9075424|FD-Score:-3.92|P-value:1.40E-4 ID:SGTC_3146|Compound:9095609|FD-Score:-3.90|P-value:1.53E-4 ID:SGTC_3147|Compound:9097027|FD-Score:-4.26|P-value:3.83E-5 ID:SGTC_3150|Compound:9097574|FD-Score:4.19|P-value:2.18E-5 ID:SGTC_3181|Compound:9108277|FD-Score:5.12|P-value:3.25E-7 ID:SGTC_3211|Compound:9128781|FD-Score:8.70|P-value:2.61E-17 ID:SGTC_3266|Compound:9136860|FD-Score:-3.50|P-value:6.25E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:-3.66|P-value:3.63E-4 ID:SGTC_86|Compound:0417-1630|FD-Score:-3.80|P-value:2.20E-4 ID:SGTC_121|Compound:0557-0495|FD-Score:-4.15|P-value:5.92E-5 ID:SGTC_255|Compound:tunicamycin|FD-Score:6.42|P-value:2.58E-10 ID:SGTC_260|Compound:nsc-65238|FD-Score:-3.40|P-value:8.50E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:-3.76|P-value:2.49E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:-3.75|P-value:2.57E-4 ID:SGTC_408|Compound:cantharidin|FD-Score:4.58|P-value:4.15E-6 ID:SGTC_528|Compound:3492-0019|FD-Score:-4.02|P-value:9.79E-5 ID:SGTC_617|Compound:3770-0098|FD-Score:3.30|P-value:5.96E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:5.97|P-value:3.76E-9 ID:SGTC_641|Compound:4092-0595|FD-Score:3.24|P-value:7.29E-4 ID:SGTC_660|Compound:4031-1742|FD-Score:3.32|P-value:5.61E-4 ID:SGTC_791|Compound:1348-1292|FD-Score:3.62|P-value:1.92E-4 ID:SGTC_794|Compound:k038-0031|FD-Score:-3.94|P-value:1.32E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:7.04|P-value:5.13E-12 ID:SGTC_914|Compound:3820-6779|FD-Score:-4.23|P-value:4.32E-5 ID:SGTC_991|Compound:3996-0057|FD-Score:3.54|P-value:2.60E-4 ID:SGTC_1234|Compound:0326-0494|FD-Score:-3.84|P-value:1.90E-4 ID:SGTC_1325|Compound:1431-0015|FD-Score:-3.67|P-value:3.46E-4 ID:SGTC_1326|Compound:1431-2094|FD-Score:-3.51|P-value:5.88E-4 ID:SGTC_1341|Compound:1486-1360|FD-Score:-6.15|P-value:4.31E-9 ID:SGTC_1348|Compound:1493-0373|FD-Score:3.36|P-value:4.80E-4 ID:SGTC_1450|Compound:4112-4260|FD-Score:3.76|P-value:1.17E-4 ID:SGTC_1489|Compound:4065-0444|FD-Score:3.63|P-value:1.91E-4 ID:SGTC_1517|Compound:deoxycholic acid methyl ester|FD-Score:3.31|P-value:5.83E-4 ID:SGTC_1521|Compound:st012942|FD-Score:4.15|P-value:2.53E-5 ID:SGTC_1529|Compound:apigenin|FD-Score:3.24|P-value:7.28E-4 ID:SGTC_1635|Compound:st007035|FD-Score:4.02|P-value:4.32E-5 ID:SGTC_1712|Compound:st032288|FD-Score:-4.40|P-value:2.20E-5 ID:SGTC_1789|Compound:tunicamycin|FD-Score:3.20|P-value:8.24E-4 ID:SGTC_1818|Compound:st052136|FD-Score:-3.82|P-value:2.05E-4 ID:SGTC_1891|Compound:st059073|FD-Score:3.75|P-value:1.22E-4 ID:SGTC_2300|Compound:7976697|FD-Score:-3.39|P-value:8.81E-4 ID:SGTC_2309|Compound:7664088|FD-Score:3.39|P-value:4.35E-4 ID:SGTC_2318|Compound:6029776|FD-Score:4.78|P-value:1.66E-6 ID:SGTC_2442|Compound:5158906|FD-Score:-4.35|P-value:2.71E-5 ID:SGTC_2489|Compound:cantharidin|FD-Score:4.53|P-value:5.05E-6 ID:SGTC_2662|Compound:daunorubicin|FD-Score:3.43|P-value:3.87E-4 ID:SGTC_2689|Compound:tacrolimus|FD-Score:4.19|P-value:2.13E-5 ID:SGTC_2694|Compound:st077685|FD-Score:-3.60|P-value:4.45E-4 ID:SGTC_2780|Compound:5215298|FD-Score:4.32|P-value:1.29E-5 ID:SGTC_2798|Compound:7813637|FD-Score:3.85|P-value:8.48E-5 ID:SGTC_2962|Compound:9082311|FD-Score:-3.54|P-value:5.46E-4 ID:SGTC_2964|Compound:9083345|FD-Score:3.79|P-value:1.04E-4 ID:SGTC_2975|Compound:9084847|FD-Score:4.65|P-value:2.94E-6 ID:SGTC_2986|Compound:9022445|FD-Score:4.47|P-value:6.61E-6 ID:SGTC_3002|Compound:9075603|FD-Score:3.94|P-value:5.83E-5 ID:SGTC_3006|Compound:9075424|FD-Score:-3.92|P-value:1.40E-4 ID:SGTC_3146|Compound:9095609|FD-Score:-3.90|P-value:1.53E-4 ID:SGTC_3147|Compound:9097027|FD-Score:-4.26|P-value:3.83E-5 ID:SGTC_3150|Compound:9097574|FD-Score:4.19|P-value:2.18E-5 ID:SGTC_3181|Compound:9108277|FD-Score:5.12|P-value:3.25E-7 ID:SGTC_3211|Compound:9128781|FD-Score:8.70|P-value:2.61E-17 ID:SGTC_3266|Compound:9136860|FD-Score:-3.50|P-value:6.25E-4

Top fitness defect scores for YBR162C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3211 9128781 8.70 49.5 µM 2.61E-17 10 1.65E-18
2 SGTC_800 3448-7362 7.04 155.0 µM 5.13E-12 translation 24 9.48E-13
3 SGTC_255 tunicamycin 6.42 200.5 nM 2.58E-10 unfolded protein response 26 6.67E-11
4 SGTC_625 k213-0058 5.97 121.0 µM 3.76E-9 translation 35 1.21E-9
5 SGTC_3181 9108277 5.12 49.5 µM 3.25E-7 iron homeostasis 2 1.51E-7
6 SGTC_2318 6029776 4.78 164.0 µM 1.66E-6 11 8.78E-7
7 SGTC_2975 9084847 4.65 53.7 µM 2.94E-6 10 1.63E-6
8 SGTC_408 cantharidin 4.58 100.0 µM 4.15E-6 cell wall signaling 13 2.35E-6
9 SGTC_2489 cantharidin 4.53 73.4 µM 5.05E-6 cell wall signaling 29 2.91E-6
10 SGTC_2986 9022445 4.47 71.4 µM 6.61E-6 8 3.88E-6
11 SGTC_2780 5215298 4.32 71.4 µM 1.29E-5 27 7.95E-6
12 SGTC_2689 tacrolimus 4.19 100.0 µM 2.13E-5 42 1.37E-5
13 SGTC_3150 9097574 4.19 49.5 µM 2.18E-5 8 1.40E-5
14 SGTC_1521 st012942 4.15 49.4 µM 2.53E-5 27 1.65E-5
15 SGTC_1635 st007035 4.02 42.4 µM 4.32E-5 207 2.92E-5
16 SGTC_3002 9075603 3.94 71.4 µM 5.83E-5 24 4.03E-5
17 SGTC_2798 7813637 3.85 45.5 µM 8.48E-5 36 6.02E-5
18 SGTC_2964 9083345 3.79 53.4 µM 1.04E-4 22 7.51E-5
19 SGTC_1450 4112-4260 3.76 37.6 µM 1.17E-4 calcium & mitochondrial duress 35 8.52E-5
20 SGTC_1891 st059073 3.75 26.2 µM 1.22E-4 TSC3-RPN4 25 8.87E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.195 5.51E-30 YPL208W RKM1 hom SET-domain lysine-N-methyltransferase, catalyzes the formation of dimethyllysine residues on the large ribsomal subunit proteins L23a (Rpl23Ap and Rpl23Bp) and L18 (Rps18Ap and Rps18Bp)
0.180 6.64E-26 YGL250W RMR1 hom Protein required for meiotic recombination and gene conversion; null mutant displays reduced PIS1 expression and growth defects on non-fermentable carbon sources and minimal media; GFP-fusion protein localizes to both cytoplasm and nucleus
0.172 1.04E-23 YBR243C ALG7 het UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin
0.171 2.09E-23 YIL039W TED1 hom Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
0.164 1.69E-21 YGL251C HFM1 hom Meiosis specific DNA helicase involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity
0.155 1.58E-19 YDR516C EMI2 hom Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
0.153 5.57E-19 YER069W ARG5,6 hom Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine
0.144 6.16E-17 YOL100W PKH2 hom Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p
0.141 2.92E-16 YLR048W RPS0B hom Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2
0.137 1.41E-15 YNL287W SEC21 het Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo
0.133 1.09E-14 YOR085W OST3 hom Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins
0.129 8.28E-14 YDR281C PHM6 hom Protein of unknown function, expression is regulated by phosphate levels
0.124 6.04E-13 YGL168W HUR1 hom Protein of unknown function; reported null mutant phenotype of hydroxyurea sensitivity may be due to effects on overlapping PMR1 gene
0.122 1.25E-12 YDL145C COP1 het Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway
0.120 3.13E-12 YIR021W-A_p YIR021W-A_p hom Putative protein of unknown function; identified by expression profiling and mass spectrometry