YBR171W / SEC66

Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR171W / SEC66

Click on Significant Values for Screen Details ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:7.34|P-value:2.24E-9 ID:SGTC_59|Compound:0330-0160|FD-Score:4.49|P-value:2.34E-4 ID:SGTC_112|Compound:phenothiazine|FD-Score:4.02|P-value:9.44E-4 ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:4.16|P-value:6.35E-4 ID:SGTC_240|Compound:5hr heat shock (37°C) + Streptovitacin|FD-Score:6.37|P-value:2.13E-7 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:9.20|P-value:6.78E-14 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:4.78|P-value:9.39E-5 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:4.32|P-value:3.99E-4 ID:SGTC_283|Compound:0987-0079|FD-Score:5.35|P-value:1.26E-5 ID:SGTC_301|Compound:4119-0100|FD-Score:4.12|P-value:7.05E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-5.07|P-value:4.63E-6 ID:SGTC_336|Compound:4111-0029|FD-Score:4.48|P-value:2.44E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:4.35|P-value:3.67E-4 ID:SGTC_580|Compound:1123-0028|FD-Score:5.57|P-value:5.66E-6 ID:SGTC_676|Compound:falcarinol|FD-Score:8.41|P-value:7.67E-12 ID:SGTC_720|Compound:r052-1644|FD-Score:5.99|P-value:1.08E-6 ID:SGTC_743|Compound:1275-0778|FD-Score:4.26|P-value:4.78E-4 ID:SGTC_759|Compound:3486-0358|FD-Score:5.55|P-value:6.09E-6 ID:SGTC_775|Compound:4577-0580|FD-Score:4.16|P-value:6.41E-4 ID:SGTC_781|Compound:4335-2806|FD-Score:5.11|P-value:3.02E-5 ID:SGTC_836|Compound:0330-0104|FD-Score:6.28|P-value:3.19E-7 ID:SGTC_842|Compound:0271-0008|FD-Score:8.73|P-value:1.16E-12 ID:SGTC_932|Compound:2857-0803|FD-Score:5.04|P-value:3.88E-5 ID:SGTC_975|Compound:1335-0046|FD-Score:4.01|P-value:9.84E-4 ID:SGTC_985|Compound:1493-0307|FD-Score:4.75|P-value:1.03E-4 ID:SGTC_1034|Compound:k072-0165|FD-Score:4.79|P-value:8.88E-5 ID:SGTC_1037|Compound:k284-1091|FD-Score:4.01|P-value:9.82E-4 ID:SGTC_1047|Compound:2043-3044|FD-Score:4.22|P-value:5.30E-4 ID:SGTC_1116|Compound:jasmonic acid|FD-Score:4.27|P-value:4.57E-4 ID:SGTC_1147|Compound:3809-1045|FD-Score:4.63|P-value:1.49E-4 ID:SGTC_1150|Compound:3909-7983|FD-Score:5.72|P-value:3.18E-6 ID:SGTC_1234|Compound:0326-0494|FD-Score:5.51|P-value:7.15E-6 ID:SGTC_1263|Compound:0774-1208|FD-Score:4.72|P-value:1.11E-4 ID:SGTC_1316|Compound:1272-0050|FD-Score:4.72|P-value:1.14E-4 ID:SGTC_1345|Compound:1488-0192|FD-Score:4.21|P-value:5.55E-4 ID:SGTC_1370|Compound:2001-0092|FD-Score:5.16|P-value:2.52E-5 ID:SGTC_1446|Compound:4239-0190|FD-Score:4.85|P-value:7.27E-5 ID:SGTC_1510|Compound:1123-0200|FD-Score:11.40|P-value:1.10E-20 ID:SGTC_1660|Compound:st012960|FD-Score:6.22|P-value:4.04E-7 ID:SGTC_1687|Compound:st020778|FD-Score:4.78|P-value:9.44E-5 ID:SGTC_1696|Compound:st025591|FD-Score:4.10|P-value:7.45E-4 ID:SGTC_1750|Compound:st081859|FD-Score:5.75|P-value:2.77E-6 ID:SGTC_1826|Compound:st055655|FD-Score:4.36|P-value:3.50E-4 ID:SGTC_1834|Compound:st055981|FD-Score:-3.81|P-value:3.53E-4 ID:SGTC_1880|Compound:MG-132|FD-Score:5.50|P-value:7.28E-6 ID:SGTC_1889|Compound:st059080|FD-Score:4.95|P-value:5.29E-5 ID:SGTC_1914|Compound:st062367|FD-Score:-3.48|P-value:9.32E-4 ID:SGTC_2157|Compound:5649910|FD-Score:5.61|P-value:4.78E-6 ID:SGTC_2243|Compound:6634756|FD-Score:4.13|P-value:6.92E-4 ID:SGTC_2307|Compound:7538926|FD-Score:4.25|P-value:4.87E-4 ID:SGTC_2358|Compound:9037157|FD-Score:4.38|P-value:3.30E-4 ID:SGTC_2526|Compound:patulin|FD-Score:5.23|P-value:1.98E-5 ID:SGTC_2579|Compound:2',5'-dihydroxy-4-methoxychalcone|FD-Score:-3.88|P-value:2.83E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:4.14|P-value:6.64E-4 ID:SGTC_2645|Compound:abamectin|FD-Score:5.96|P-value:1.22E-6 ID:SGTC_2673|Compound:kobusone|FD-Score:4.13|P-value:6.97E-4 ID:SGTC_2701|Compound:st077767|FD-Score:12.10|P-value:6.29E-23 ID:SGTC_2782|Compound:7736369|FD-Score:4.37|P-value:3.43E-4 ID:SGTC_2844|Compound:9010103|FD-Score:5.21|P-value:2.14E-5 ID:SGTC_2884|Compound:9058502|FD-Score:5.74|P-value:2.94E-6 ID:SGTC_2893|Compound:9048488|FD-Score:-4.09|P-value:1.45E-4 ID:SGTC_2898|Compound:9056270|FD-Score:4.19|P-value:5.82E-4 ID:SGTC_2981|Compound:9013952|FD-Score:5.33|P-value:1.38E-5 ID:SGTC_3039|Compound:9090270|FD-Score:6.09|P-value:7.04E-7 ID:SGTC_3056|Compound:9094900|FD-Score:4.07|P-value:8.31E-4 ID:SGTC_3166|Compound:9102297|FD-Score:5.53|P-value:6.47E-6 ID:SGTC_3168|Compound:9103419|FD-Score:7.08|P-value:8.44E-9 ID:SGTC_3176|Compound:9106582|FD-Score:4.56|P-value:1.87E-4 ID:SGTC_3186|Compound:9110464|FD-Score:5.04|P-value:3.87E-5 ID:SGTC_3202|Compound:9113761|FD-Score:4.19|P-value:5.86E-4 ID:SGTC_3212|Compound:9128307|FD-Score:9.13|P-value:1.00E-13 ID:SGTC_3225|Compound:9130819|FD-Score:4.44|P-value:2.77E-4 ID:SGTC_3323|Compound:9138564|FD-Score:4.82|P-value:8.26E-5 ID:SGTC_3327|Compound:9140648|FD-Score:-3.63|P-value:6.05E-4 ID:SGTC_3330|Compound:9141424|FD-Score:8.81|P-value:7.18E-13 ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:7.34|P-value:2.24E-9 ID:SGTC_59|Compound:0330-0160|FD-Score:4.49|P-value:2.34E-4 ID:SGTC_112|Compound:phenothiazine|FD-Score:4.02|P-value:9.44E-4 ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:4.16|P-value:6.35E-4 ID:SGTC_240|Compound:5hr heat shock (37°C) + Streptovitacin|FD-Score:6.37|P-value:2.13E-7 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:9.20|P-value:6.78E-14 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:4.78|P-value:9.39E-5 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:4.32|P-value:3.99E-4 ID:SGTC_283|Compound:0987-0079|FD-Score:5.35|P-value:1.26E-5 ID:SGTC_301|Compound:4119-0100|FD-Score:4.12|P-value:7.05E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-5.07|P-value:4.63E-6 ID:SGTC_336|Compound:4111-0029|FD-Score:4.48|P-value:2.44E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:4.35|P-value:3.67E-4 ID:SGTC_580|Compound:1123-0028|FD-Score:5.57|P-value:5.66E-6 ID:SGTC_676|Compound:falcarinol|FD-Score:8.41|P-value:7.67E-12 ID:SGTC_720|Compound:r052-1644|FD-Score:5.99|P-value:1.08E-6 ID:SGTC_743|Compound:1275-0778|FD-Score:4.26|P-value:4.78E-4 ID:SGTC_759|Compound:3486-0358|FD-Score:5.55|P-value:6.09E-6 ID:SGTC_775|Compound:4577-0580|FD-Score:4.16|P-value:6.41E-4 ID:SGTC_781|Compound:4335-2806|FD-Score:5.11|P-value:3.02E-5 ID:SGTC_836|Compound:0330-0104|FD-Score:6.28|P-value:3.19E-7 ID:SGTC_842|Compound:0271-0008|FD-Score:8.73|P-value:1.16E-12 ID:SGTC_932|Compound:2857-0803|FD-Score:5.04|P-value:3.88E-5 ID:SGTC_975|Compound:1335-0046|FD-Score:4.01|P-value:9.84E-4 ID:SGTC_985|Compound:1493-0307|FD-Score:4.75|P-value:1.03E-4 ID:SGTC_1034|Compound:k072-0165|FD-Score:4.79|P-value:8.88E-5 ID:SGTC_1037|Compound:k284-1091|FD-Score:4.01|P-value:9.82E-4 ID:SGTC_1047|Compound:2043-3044|FD-Score:4.22|P-value:5.30E-4 ID:SGTC_1116|Compound:jasmonic acid|FD-Score:4.27|P-value:4.57E-4 ID:SGTC_1147|Compound:3809-1045|FD-Score:4.63|P-value:1.49E-4 ID:SGTC_1150|Compound:3909-7983|FD-Score:5.72|P-value:3.18E-6 ID:SGTC_1234|Compound:0326-0494|FD-Score:5.51|P-value:7.15E-6 ID:SGTC_1263|Compound:0774-1208|FD-Score:4.72|P-value:1.11E-4 ID:SGTC_1316|Compound:1272-0050|FD-Score:4.72|P-value:1.14E-4 ID:SGTC_1345|Compound:1488-0192|FD-Score:4.21|P-value:5.55E-4 ID:SGTC_1370|Compound:2001-0092|FD-Score:5.16|P-value:2.52E-5 ID:SGTC_1446|Compound:4239-0190|FD-Score:4.85|P-value:7.27E-5 ID:SGTC_1510|Compound:1123-0200|FD-Score:11.40|P-value:1.10E-20 ID:SGTC_1660|Compound:st012960|FD-Score:6.22|P-value:4.04E-7 ID:SGTC_1687|Compound:st020778|FD-Score:4.78|P-value:9.44E-5 ID:SGTC_1696|Compound:st025591|FD-Score:4.10|P-value:7.45E-4 ID:SGTC_1750|Compound:st081859|FD-Score:5.75|P-value:2.77E-6 ID:SGTC_1826|Compound:st055655|FD-Score:4.36|P-value:3.50E-4 ID:SGTC_1834|Compound:st055981|FD-Score:-3.81|P-value:3.53E-4 ID:SGTC_1880|Compound:MG-132|FD-Score:5.50|P-value:7.28E-6 ID:SGTC_1889|Compound:st059080|FD-Score:4.95|P-value:5.29E-5 ID:SGTC_1914|Compound:st062367|FD-Score:-3.48|P-value:9.32E-4 ID:SGTC_2157|Compound:5649910|FD-Score:5.61|P-value:4.78E-6 ID:SGTC_2243|Compound:6634756|FD-Score:4.13|P-value:6.92E-4 ID:SGTC_2307|Compound:7538926|FD-Score:4.25|P-value:4.87E-4 ID:SGTC_2358|Compound:9037157|FD-Score:4.38|P-value:3.30E-4 ID:SGTC_2526|Compound:patulin|FD-Score:5.23|P-value:1.98E-5 ID:SGTC_2579|Compound:2',5'-dihydroxy-4-methoxychalcone|FD-Score:-3.88|P-value:2.83E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:4.14|P-value:6.64E-4 ID:SGTC_2645|Compound:abamectin|FD-Score:5.96|P-value:1.22E-6 ID:SGTC_2673|Compound:kobusone|FD-Score:4.13|P-value:6.97E-4 ID:SGTC_2701|Compound:st077767|FD-Score:12.10|P-value:6.29E-23 ID:SGTC_2782|Compound:7736369|FD-Score:4.37|P-value:3.43E-4 ID:SGTC_2844|Compound:9010103|FD-Score:5.21|P-value:2.14E-5 ID:SGTC_2884|Compound:9058502|FD-Score:5.74|P-value:2.94E-6 ID:SGTC_2893|Compound:9048488|FD-Score:-4.09|P-value:1.45E-4 ID:SGTC_2898|Compound:9056270|FD-Score:4.19|P-value:5.82E-4 ID:SGTC_2981|Compound:9013952|FD-Score:5.33|P-value:1.38E-5 ID:SGTC_3039|Compound:9090270|FD-Score:6.09|P-value:7.04E-7 ID:SGTC_3056|Compound:9094900|FD-Score:4.07|P-value:8.31E-4 ID:SGTC_3166|Compound:9102297|FD-Score:5.53|P-value:6.47E-6 ID:SGTC_3168|Compound:9103419|FD-Score:7.08|P-value:8.44E-9 ID:SGTC_3176|Compound:9106582|FD-Score:4.56|P-value:1.87E-4 ID:SGTC_3186|Compound:9110464|FD-Score:5.04|P-value:3.87E-5 ID:SGTC_3202|Compound:9113761|FD-Score:4.19|P-value:5.86E-4 ID:SGTC_3212|Compound:9128307|FD-Score:9.13|P-value:1.00E-13 ID:SGTC_3225|Compound:9130819|FD-Score:4.44|P-value:2.77E-4 ID:SGTC_3323|Compound:9138564|FD-Score:4.82|P-value:8.26E-5 ID:SGTC_3327|Compound:9140648|FD-Score:-3.63|P-value:6.05E-4 ID:SGTC_3330|Compound:9141424|FD-Score:8.81|P-value:7.18E-13

Top fitness defect scores for YBR171W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2701 st077767 12.10 14.7 µM 6.29E-23 3 7.15E-34
2 SGTC_1510 1123-0200 11.40 71.5 µM 1.10E-20 4 1.51E-30
3 SGTC_241 5hr heat shock (37°C) + cantharidin 9.20 155.4 µM 6.78E-14 cell wall signaling 14 1.86E-20
4 SGTC_3212 9128307 9.13 49.5 µM 1.00E-13 3 3.33E-20
5 SGTC_3330 9141424 8.81 71.4 µM 7.18E-13 amide catabolism 2 6.24E-19
6 SGTC_842 0271-0008 8.73 11.1 µM 1.16E-12 heme biosynthesis & mitochondrial translocase 3 1.27E-18
7 SGTC_676 falcarinol 8.41 140.0 µM 7.67E-12 7 2.13E-17
8 SGTC_28 pentachloropyridine N-oxide 7.34 1.6 µM 2.24E-9 TSC3-RPN4 7 1.03E-13
9 SGTC_3168 9103419 7.08 49.5 µM 8.44E-9 60S ribosome export 1 7.47E-13
10 SGTC_240 5hr heat shock (37°C) + Streptovitacin 6.37 300.0 µM 2.13E-7 heat shock/prefoldin 13 9.41E-11
11 SGTC_836 0330-0104 6.28 1.4 µM 3.19E-7 3 1.72E-10
12 SGTC_1660 st012960 6.22 28.1 µM 4.04E-7 iron homeostasis 4 2.46E-10
13 SGTC_3039 9090270 6.09 49.5 µM 7.04E-7 RPP1 & pyrimidine depletion 2 5.65E-10
14 SGTC_720 r052-1644 5.99 49.9 µM 1.08E-6 TSC3-RPN4 8 1.07E-9
15 SGTC_2645 abamectin 5.96 100.0 µM 1.22E-6 heme requiring 10 1.29E-9
16 SGTC_1750 st081859 5.75 2.4 µM 2.77E-6 endomembrane recycling 113 4.42E-9
17 SGTC_2884 9058502 5.74 52.0 µM 2.94E-6 6 4.85E-9
18 SGTC_1150 3909-7983 5.72 10.9 µM 3.18E-6 4 5.45E-9
19 SGTC_2157 5649910 5.61 96.4 µM 4.78E-6 4 1.00E-8
20 SGTC_580 1123-0028 5.57 41.0 µM 5.66E-6 DNA damage response 5 1.30E-8

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.196 1.75E-30 YLR027C AAT2 hom Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells
0.162 3.38E-21 YDR260C SWM1 hom Subunit of the anaphase-promoting complex, which is an E3 ubiquitin ligase that regulates the metaphase-anaphase transition and exit from mitosis; required for activation of the daughter-specific gene expression and spore wall maturation
0.146 2.53E-17 YDL020C RPN4 hom Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
0.145 3.91E-17 YDR086C SSS1 het Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p
0.144 6.51E-17 YCR063W BUD31 hom Component of the SF3b subcomplex of the U2 snRNP; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis
0.143 7.44E-17 YLR200W YKE2 hom Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin
0.139 8.08E-16 YER107C GLE2 hom RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1
0.127 1.87E-13 YCR064C_d YCR064C_d hom Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31
0.122 1.32E-12 YHR209W CRG1 hom S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin
0.121 2.44E-12 YBR058C-A TSC3 hom Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
0.116 1.62E-11 YMR161W HLJ1 hom Co-chaperone for Hsp40p, anchored in the ER membrane; with its homolog Ydj1p promotes ER-associated protein degradation (ERAD) of integral membrane substrates; similar to E. coli DnaJ
0.115 2.37E-11 YAL012W CYS3 hom Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress
0.112 8.29E-11 YDR457W TOM1 hom E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase
0.109 2.31E-10 YOR001W RRP6 hom Nuclear exosome exonuclease component; has 3'-5' exonuclease activity; involved in RNA processing, maturation, surveillance, degradation, tethering, and export; has similarity to E. coli RNase D and to human PM-Sc1 100 (EXOSC10); mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay
0.106 6.55E-10 YDR323C PEP7 hom Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance