HIPHOP chemogenomics database

YBR181C / RPS6B

Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6B has a paralog, RPS6A, that arose from the whole genome duplication

Zygosity: Homozygous strain

fixedexpanded
Profile for YBR181C / RPS6B

Click on Significant Values for Screen Details

Top fitness defect scores for YBR181C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_1991	st073000	7.41	8.9 µM	2.34E-14	NEO1	19	6.29E-14
2	SGTC_2688	kanamycin b	5.72	96.7 µM	3.04E-9	calcium & mitochondrial duress	177	5.35E-9
3	SGTC_2639	lactacystin	5.31	53.0 µM	3.39E-8		8	5.47E-8
4	SGTC_2576	guaiol	4.75	23.1 µM	7.04E-7		8	1.02E-6
5	SGTC_406	estrone	4.58	100.0 µM	1.68E-6		5	2.37E-6
6	SGTC_1460	k062-0059	4.36	9.2 µM	4.77E-6	Golgi	7	6.49E-6
7	SGTC_2347	9049783	4.36	174.8 µM	4.77E-6		15	6.50E-6
8	SGTC_1365	1672-3266	4.07	55.4 µM	1.81E-5		15	2.36E-5
9	SGTC_1468	k284-1086	3.89	134.0 µM	3.92E-5	DNA intercalators	39	4.98E-5
10	SGTC_2376	9072922	3.85	81.3 µM	4.76E-5		32	6.01E-5
11	SGTC_1343	1487-0095	3.64	224.0 µM	1.13E-4		11	1.38E-4
12	SGTC_1328	1441-0025	3.52	69.2 µM	1.77E-4		13	2.14E-4
13	SGTC_1781	st038725	3.51	66.3 µM	1.86E-4	NEO1	122	2.25E-4
14	SGTC_1545	lovastatin	3.47	49.4 µM	2.13E-4		41	2.56E-4
15	SGTC_2789	5281112	3.40	71.4 µM	2.84E-4		66	3.38E-4
16	SGTC_2289	7946404	3.39	165.1 µM	2.93E-4		117	3.49E-4
17	SGTC_3218	9131285	3.38	49.5 µM	3.05E-4	endomembrane recycling	76	3.62E-4
18	SGTC_1348	1493-0373	3.38	93.8 µM	3.10E-4		28	3.68E-4
19	SGTC_2439	5492030	3.32	7.2 µM	3.84E-4		33	4.52E-4
20	SGTC_1984	st072354	3.31	53.1 µM	4.03E-4		30	4.75E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.187	9.21E-28	YJL136C	RPS21B	hom	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21B has a paralog, RPS21A, that arose from the whole genome duplication
0.177	5.76E-25	YOL159C-A	YOL159C-A	hom	Protein of unknown function; overexpression affects endocytic protein trafficking; identified by sequence comparison with hemiascomycetous yeast species
0.154	2.58E-19	YOR293W	RPS10A	hom	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10A has a paralog, RPS10B, that arose from the whole genome duplication
0.142	1.68E-16	YDR371W_p	CTS2_p	hom	Putative chitinase, functionally complements A. gossypii cts2 mutant sporulation defect
0.138	9.67E-16	YKL061W_p	BLI1_p	hom	Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome
0.137	1.71E-15	YHR203C	RPS4B	hom	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4B has a paralog, RPS4A, that arose from the whole genome duplication
0.135	3.41E-15	YIL048W	NEO1	het	Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus
0.133	1.31E-14	YCR064C_d	YCR064C_d	hom	Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31
0.131	2.57E-14	YMR143W	RPS16A	hom	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16A has a paralog, RPS16B, that arose from the whole genome duplication
0.126	2.28E-13	YDR372C	VPS74	hom	Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33
0.124	6.32E-13	YJL052W	TDH1	hom	Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress
0.123	8.54E-13	YDR456W	NHX1	hom	Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion
0.120	3.01E-12	YDL115C	IWR1	hom	RNA polymerase II transport factor, conserved from yeast to humans; also has a role in transporting RNA polymerase III into the nucleus; interacts with most of the RNAP II subunits; nucleo-cytoplasmic shuttling protein; deletion causes hypersensitivity to K1 killer toxin; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress
0.116	1.48E-11	YOR006C	TSR3	hom	Protein required for 20S pre-rRNA processing; involved in processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress
0.114	3.88E-11	YLR264W	RPS28B	hom	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S28, no bacterial homolog; RPS28B has a paralog, RPS28A, that arose from the whole genome duplication