YBR186W / PCH2

Nucleolar component of the pachytene checkpoint, which prevents chromosome segregation when recombination and chromosome synapsis are defective; also represses meiotic interhomolog recombination in the rDNA; required for meiotic double-stranded break formation

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR186W / PCH2

Click on Significant Values for Screen Details ID:SGTC_56|Compound:0957-0365|FD-Score:2.75|P-value:7.07E-4 ID:SGTC_57|Compound:miconazole|FD-Score:3.81|P-value:5.11E-6 ID:SGTC_607|Compound:4491-0986|FD-Score:2.88|P-value:4.08E-4 ID:SGTC_749|Compound:1000-0129|FD-Score:5.04|P-value:2.87E-9 ID:SGTC_1031|Compound:k035-0009|FD-Score:3.04|P-value:2.08E-4 ID:SGTC_1037|Compound:k284-1091|FD-Score:-3.68|P-value:1.33E-5 ID:SGTC_1061|Compound:3448-9325|FD-Score:3.13|P-value:1.42E-4 ID:SGTC_1170|Compound:0310-0127|FD-Score:4.16|P-value:7.53E-7 ID:SGTC_1303|Compound:1160-0052|FD-Score:2.84|P-value:4.86E-4 ID:SGTC_1315|Compound:1237-0019|FD-Score:2.70|P-value:8.57E-4 ID:SGTC_1420|Compound:4'-methoxyflavone|FD-Score:2.68|P-value:9.13E-4 ID:SGTC_1529|Compound:apigenin|FD-Score:3.35|P-value:5.25E-5 ID:SGTC_1644|Compound:st010134|FD-Score:2.94|P-value:3.22E-4 ID:SGTC_1648|Compound:st011736|FD-Score:3.19|P-value:1.10E-4 ID:SGTC_1748|Compound:st037455|FD-Score:3.19|P-value:1.06E-4 ID:SGTC_2115|Compound:tiliquinol|FD-Score:2.80|P-value:5.78E-4 ID:SGTC_2493|Compound:resveratrol|FD-Score:3.04|P-value:2.13E-4 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:2.69|P-value:8.96E-4 ID:SGTC_2801|Compound:7907800|FD-Score:-3.07|P-value:2.29E-4 ID:SGTC_3207|Compound:9114163|FD-Score:2.95|P-value:3.13E-4 ID:SGTC_56|Compound:0957-0365|FD-Score:2.75|P-value:7.07E-4 ID:SGTC_57|Compound:miconazole|FD-Score:3.81|P-value:5.11E-6 ID:SGTC_607|Compound:4491-0986|FD-Score:2.88|P-value:4.08E-4 ID:SGTC_749|Compound:1000-0129|FD-Score:5.04|P-value:2.87E-9 ID:SGTC_1031|Compound:k035-0009|FD-Score:3.04|P-value:2.08E-4 ID:SGTC_1037|Compound:k284-1091|FD-Score:-3.68|P-value:1.33E-5 ID:SGTC_1061|Compound:3448-9325|FD-Score:3.13|P-value:1.42E-4 ID:SGTC_1170|Compound:0310-0127|FD-Score:4.16|P-value:7.53E-7 ID:SGTC_1303|Compound:1160-0052|FD-Score:2.84|P-value:4.86E-4 ID:SGTC_1315|Compound:1237-0019|FD-Score:2.70|P-value:8.57E-4 ID:SGTC_1420|Compound:4'-methoxyflavone|FD-Score:2.68|P-value:9.13E-4 ID:SGTC_1529|Compound:apigenin|FD-Score:3.35|P-value:5.25E-5 ID:SGTC_1644|Compound:st010134|FD-Score:2.94|P-value:3.22E-4 ID:SGTC_1648|Compound:st011736|FD-Score:3.19|P-value:1.10E-4 ID:SGTC_1748|Compound:st037455|FD-Score:3.19|P-value:1.06E-4 ID:SGTC_2115|Compound:tiliquinol|FD-Score:2.80|P-value:5.78E-4 ID:SGTC_2493|Compound:resveratrol|FD-Score:3.04|P-value:2.13E-4 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:2.69|P-value:8.96E-4 ID:SGTC_2801|Compound:7907800|FD-Score:-3.07|P-value:2.29E-4 ID:SGTC_3207|Compound:9114163|FD-Score:2.95|P-value:3.13E-4

Top fitness defect scores for YBR186W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_749 1000-0129 5.04 128.0 µM 2.87E-9 amide catabolism 5 2.35E-7
2 SGTC_1170 0310-0127 4.16 64.8 µM 7.53E-7 21 1.62E-5
3 SGTC_57 miconazole 3.81 162.5 nM 5.11E-6 ergosterol depletion effects on membrane 27 6.93E-5
4 SGTC_1529 apigenin 3.35 74.0 µM 5.25E-5 55 4.09E-4
5 SGTC_1748 st037455 3.19 53.6 µM 1.06E-4 70 7.00E-4
6 SGTC_1648 st011736 3.19 69.8 µM 1.10E-4 PDR1 37 7.21E-4
7 SGTC_1061 3448-9325 3.13 110.0 µM 1.42E-4 47 8.76E-4
8 SGTC_1031 k035-0009 3.04 16.0 µM 2.08E-4 74 0.00117
9 SGTC_2493 resveratrol 3.04 26.1 µM 2.13E-4 31 0.00119
10 SGTC_3207 9114163 2.95 49.5 µM 3.13E-4 84 0.00160
11 SGTC_1644 st010134 2.94 20.9 µM 3.22E-4 mitochondrial processes 36 0.00163
12 SGTC_607 4491-0986 2.88 66.8 µM 4.08E-4 heme requiring 77 0.00196
13 SGTC_1303 1160-0052 2.84 261.0 µM 4.86E-4 53 0.00224
14 SGTC_2115 tiliquinol 2.80 12.8 µM 5.78E-4 47 0.00256
15 SGTC_56 0957-0365 2.75 83.2 µM 7.07E-4 Golgi 122 0.00298
16 SGTC_1315 1237-0019 2.70 3.7 µM 8.57E-4 65 0.00346
17 SGTC_2520 obtusaquinone 2.69 4.7 µM 8.96E-4 75 0.00358
18 SGTC_1420 4'-methoxyflavone 2.68 42.0 µM 9.13E-4 65 0.00363
19 SGTC_2102 5377816 2.64 72.8 µM 0.00109 99 0.00416
20 SGTC_645 0845-0943 2.61 5.4 µM 0.00122 60S ribosome export 83 0.00452

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.159 1.67E-20 YBR089C-A NHP6B hom High-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to the chromosomes; functionally redundant with Nhp6Ap; homologous to mammalian HMGB1 and HMGB2; NHP6B has a paralog, NHP6A, that arose from the whole genome duplication
0.154 3.68E-19 YBR102C EXO84 het Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate
0.137 2.00E-15 YDR209C_d YDR209C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YDR210W.
0.134 6.84E-15 YPR055W SEC8 het Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress
0.123 9.07E-13 YGR103W NOP7 het Component of several different pre-ribosomal particles; forms a complex with Ytm1p and Erb1p that is required for maturation of the large ribosomal subunit; required for exit from G0 and the initiation of cell proliferation
0.114 3.54E-11 YGL233W SEC15 het Essential 113 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; interacts with and functions as a downstream effector of active, GTP-bound Sec4p, a Rab family GTPase
0.114 3.77E-11 YJL152W_d YJL152W_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.114 3.89E-11 YKR034W DAL80 hom Negative regulator of genes in multiple nitrogen degradation pathways; expression is regulated by nitrogen levels and by Gln3p; member of the GATA-binding family, forms homodimers and heterodimers with Gzf3p; DAL80 has a paralog, GZF3, that arose from the whole genome duplication
0.112 7.30E-11 YDR146C SWI5 hom Transcription factor that recruits Mediator and Swi/Snf complexes; activates transcription of genes expressed at the M/G1 phase boundary and in G1 phase; required for expression of the HO gene controlling mating type switching; localization to nucleus occurs during G1 and appears to be regulated by phosphorylation by Cdc28p kinase; SWI5 has a paralog, ACE2, that arose from the whole genome duplication
0.110 1.76E-10 YPL261C_d YPL261C_d hom Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YPL260W
0.110 2.13E-10 YDR119W_p VBA4_p hom Protein of unknown function with proposed role as a basic amino acid permease based on phylogeny; GFP-fusion protein localizes to vacuolar membrane; physical interaction with Atg27p suggests a possible role in autophagy; non-essential gene
0.108 4.30E-10 YER008C SEC3 het Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress
0.106 7.62E-10 YJR051W OSM1 hom Fumarate reductase, catalyzes the reduction of fumarate to succinate; required for the reoxidation of intracellular NADH under anaerobic conditions; mutations cause osmotic sensitivity; OSM1 has a paralog, FRD1, that arose from the whole genome duplication
0.105 1.18E-9 YLR137W RKM5 hom Protein lysine methyltransferase; monomethylates Lys-46 of the ribosomal large subunit Rpl1a/Rpl1b; member of the seven beta-strand methyltransferase superfamily; orthologs only found among fungal species
0.104 1.56E-9 YOR123C LEO1 hom Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay