YBR188C / NTC20

Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR188C / NTC20

Click on Significant Values for Screen Details ID:SGTC_130|Compound:0136-0219|FD-Score:-3.09|P-value:4.93E-4 ID:SGTC_173|Compound:1273-0061|FD-Score:-2.91|P-value:9.61E-4 ID:SGTC_223|Compound:busulfan|FD-Score:3.69|P-value:4.85E-5 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:3.76|P-value:3.51E-5 ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:7.17|P-value:1.66E-14 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:5.03|P-value:5.12E-8 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:2.97|P-value:8.53E-4 ID:SGTC_263|Compound:alizarin|FD-Score:3.39|P-value:1.76E-4 ID:SGTC_290|Compound:2043-6722|FD-Score:3.83|P-value:2.57E-5 ID:SGTC_314|Compound:1671-0008|FD-Score:4.73|P-value:2.93E-7 ID:SGTC_364|Compound:0083-0097|FD-Score:4.00|P-value:1.18E-5 ID:SGTC_431|Compound:k089-0097|FD-Score:3.38|P-value:1.82E-4 ID:SGTC_635|Compound:k038-0028|FD-Score:-3.45|P-value:1.20E-4 ID:SGTC_803|Compound:4488-1585|FD-Score:3.31|P-value:2.42E-4 ID:SGTC_813|Compound:k007-0158|FD-Score:3.75|P-value:3.77E-5 ID:SGTC_816|Compound:0325-0417|FD-Score:3.29|P-value:2.62E-4 ID:SGTC_826|Compound:1683-5028|FD-Score:3.15|P-value:4.36E-4 ID:SGTC_990|Compound:3985-0011|FD-Score:3.74|P-value:3.87E-5 ID:SGTC_1000|Compound:4048-0884|FD-Score:-3.10|P-value:4.76E-4 ID:SGTC_1145|Compound:3546-0597|FD-Score:3.48|P-value:1.18E-4 ID:SGTC_1251|Compound:0490-5699|FD-Score:3.50|P-value:1.11E-4 ID:SGTC_1258|Compound:0671-0043|FD-Score:3.73|P-value:4.12E-5 ID:SGTC_1368|Compound:1852-0141|FD-Score:-2.93|P-value:8.87E-4 ID:SGTC_1618|Compound:st003135|FD-Score:3.20|P-value:3.68E-4 ID:SGTC_1769|Compound:st044406|FD-Score:-3.20|P-value:3.24E-4 ID:SGTC_1794|Compound:st048918|FD-Score:3.49|P-value:1.16E-4 ID:SGTC_1819|Compound:st053448|FD-Score:-3.52|P-value:8.90E-5 ID:SGTC_1922|Compound:5578187|FD-Score:-4.76|P-value:2.09E-7 ID:SGTC_1928|Compound:4'-methoxychalcone|FD-Score:-3.90|P-value:1.61E-5 ID:SGTC_1979|Compound:st071809|FD-Score:3.22|P-value:3.44E-4 ID:SGTC_2329|Compound:6138490|FD-Score:3.37|P-value:1.83E-4 ID:SGTC_2469|Compound:5655019|FD-Score:3.63|P-value:6.32E-5 ID:SGTC_2780|Compound:5215298|FD-Score:-5.39|P-value:5.08E-9 ID:SGTC_2789|Compound:5281112|FD-Score:-3.11|P-value:4.61E-4 ID:SGTC_2916|Compound:7875993|FD-Score:3.24|P-value:3.14E-4 ID:SGTC_2975|Compound:9084847|FD-Score:3.25|P-value:2.99E-4 ID:SGTC_3093|Compound:9117344|FD-Score:3.02|P-value:7.13E-4 ID:SGTC_3201|Compound:9113999|FD-Score:-3.95|P-value:1.28E-5 ID:SGTC_3204|Compound:9114183|FD-Score:4.89|P-value:1.14E-7 ID:SGTC_3206|Compound:9114350|FD-Score:-3.79|P-value:2.77E-5 ID:SGTC_130|Compound:0136-0219|FD-Score:-3.09|P-value:4.93E-4 ID:SGTC_173|Compound:1273-0061|FD-Score:-2.91|P-value:9.61E-4 ID:SGTC_223|Compound:busulfan|FD-Score:3.69|P-value:4.85E-5 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:3.76|P-value:3.51E-5 ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:7.17|P-value:1.66E-14 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:5.03|P-value:5.12E-8 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:2.97|P-value:8.53E-4 ID:SGTC_263|Compound:alizarin|FD-Score:3.39|P-value:1.76E-4 ID:SGTC_290|Compound:2043-6722|FD-Score:3.83|P-value:2.57E-5 ID:SGTC_314|Compound:1671-0008|FD-Score:4.73|P-value:2.93E-7 ID:SGTC_364|Compound:0083-0097|FD-Score:4.00|P-value:1.18E-5 ID:SGTC_431|Compound:k089-0097|FD-Score:3.38|P-value:1.82E-4 ID:SGTC_635|Compound:k038-0028|FD-Score:-3.45|P-value:1.20E-4 ID:SGTC_803|Compound:4488-1585|FD-Score:3.31|P-value:2.42E-4 ID:SGTC_813|Compound:k007-0158|FD-Score:3.75|P-value:3.77E-5 ID:SGTC_816|Compound:0325-0417|FD-Score:3.29|P-value:2.62E-4 ID:SGTC_826|Compound:1683-5028|FD-Score:3.15|P-value:4.36E-4 ID:SGTC_990|Compound:3985-0011|FD-Score:3.74|P-value:3.87E-5 ID:SGTC_1000|Compound:4048-0884|FD-Score:-3.10|P-value:4.76E-4 ID:SGTC_1145|Compound:3546-0597|FD-Score:3.48|P-value:1.18E-4 ID:SGTC_1251|Compound:0490-5699|FD-Score:3.50|P-value:1.11E-4 ID:SGTC_1258|Compound:0671-0043|FD-Score:3.73|P-value:4.12E-5 ID:SGTC_1368|Compound:1852-0141|FD-Score:-2.93|P-value:8.87E-4 ID:SGTC_1618|Compound:st003135|FD-Score:3.20|P-value:3.68E-4 ID:SGTC_1769|Compound:st044406|FD-Score:-3.20|P-value:3.24E-4 ID:SGTC_1794|Compound:st048918|FD-Score:3.49|P-value:1.16E-4 ID:SGTC_1819|Compound:st053448|FD-Score:-3.52|P-value:8.90E-5 ID:SGTC_1922|Compound:5578187|FD-Score:-4.76|P-value:2.09E-7 ID:SGTC_1928|Compound:4'-methoxychalcone|FD-Score:-3.90|P-value:1.61E-5 ID:SGTC_1979|Compound:st071809|FD-Score:3.22|P-value:3.44E-4 ID:SGTC_2329|Compound:6138490|FD-Score:3.37|P-value:1.83E-4 ID:SGTC_2469|Compound:5655019|FD-Score:3.63|P-value:6.32E-5 ID:SGTC_2780|Compound:5215298|FD-Score:-5.39|P-value:5.08E-9 ID:SGTC_2789|Compound:5281112|FD-Score:-3.11|P-value:4.61E-4 ID:SGTC_2916|Compound:7875993|FD-Score:3.24|P-value:3.14E-4 ID:SGTC_2975|Compound:9084847|FD-Score:3.25|P-value:2.99E-4 ID:SGTC_3093|Compound:9117344|FD-Score:3.02|P-value:7.13E-4 ID:SGTC_3201|Compound:9113999|FD-Score:-3.95|P-value:1.28E-5 ID:SGTC_3204|Compound:9114183|FD-Score:4.89|P-value:1.14E-7 ID:SGTC_3206|Compound:9114350|FD-Score:-3.79|P-value:2.77E-5

Top fitness defect scores for YBR188C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_239 5hr heat shock (37°C) + mitomycin C 7.17 819.4 µM 1.66E-14 heat shock/prefoldin 8 3.82E-13
2 SGTC_241 5hr heat shock (37°C) + cantharidin 5.03 155.4 µM 5.12E-8 cell wall signaling 45 2.40E-7
3 SGTC_3204 9114183 4.89 49.5 µM 1.14E-7 12 4.92E-7
4 SGTC_314 1671-0008 4.73 2.7 µM 2.93E-7 27 1.14E-6
5 SGTC_364 0083-0097 4.00 67.7 µM 1.18E-5 22 3.14E-5
6 SGTC_290 2043-6722 3.83 48.1 µM 2.57E-5 11 6.32E-5
7 SGTC_238 5hr heat shock (37°C) + nsc-638432 3.76 8.9 µM 3.51E-5 heat shock/prefoldin 48 8.35E-5
8 SGTC_813 k007-0158 3.75 173.0 µM 3.77E-5 fatty acid desaturase (OLE1) 49 8.92E-5
9 SGTC_990 3985-0011 3.74 41.3 µM 3.87E-5 amide catabolism 29 9.11E-5
10 SGTC_1258 0671-0043 3.73 57.1 µM 4.12E-5 30 9.66E-5
11 SGTC_223 busulfan 3.69 5.0 mM 4.85E-5 11 1.12E-4
12 SGTC_2469 5655019 3.63 90.8 µM 6.32E-5 28 1.42E-4
13 SGTC_1251 0490-5699 3.50 265.0 µM 1.11E-4 54 2.36E-4
14 SGTC_1794 st048918 3.49 76.9 µM 1.16E-4 15 2.44E-4
15 SGTC_1145 3546-0597 3.48 10.2 µM 1.18E-4 mitochondrial processes 31 2.49E-4
16 SGTC_263 alizarin 3.39 107.9 µM 1.76E-4 iron homeostasis 88 3.55E-4
17 SGTC_431 k089-0097 3.38 107.0 µM 1.82E-4 iron homeostasis 69 3.66E-4
18 SGTC_2329 6138490 3.37 200.0 µM 1.83E-4 56 3.69E-4
19 SGTC_803 4488-1585 3.31 147.0 µM 2.42E-4 32 4.73E-4
20 SGTC_816 0325-0417 3.29 81.3 µM 2.62E-4 30 5.08E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.184 7.79E-27 YDR260C SWM1 hom Subunit of the anaphase-promoting complex, which is an E3 ubiquitin ligase that regulates the metaphase-anaphase transition and exit from mitosis; required for activation of the daughter-specific gene expression and spore wall maturation
0.183 1.26E-26 YLR027C AAT2 hom Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells
0.149 3.25E-18 YHR209W CRG1 hom S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin
0.148 5.14E-18 YCR063W BUD31 hom Component of the SF3b subcomplex of the U2 snRNP; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis
0.141 2.40E-16 YOR001W RRP6 hom Nuclear exosome exonuclease component; has 3'-5' exonuclease activity; involved in RNA processing, maturation, surveillance, degradation, tethering, and export; has similarity to E. coli RNase D and to human PM-Sc1 100 (EXOSC10); mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay
0.128 9.96E-14 YDR457W TOM1 hom E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase
0.111 9.65E-11 YLR200W YKE2 hom Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin
0.107 4.48E-10 YNL140C_p YNL140C_p hom Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps THO2/YNL139C
0.107 5.48E-10 YKR024C DBP7 hom Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions
0.102 3.20E-9 YNL098C RAS2 hom GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes
0.100 6.36E-9 YPL017C IRC15 hom Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci
0.098 1.09E-8 YJR020W_d YJR020W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.097 1.82E-8 YHR041C SRB2 hom Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance
0.096 2.83E-8 YJL171C YJL171C hom GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress
0.090 1.64E-7 YKL007W CAP1 hom Alpha subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress