HIPHOP chemogenomics database

YBR188C / NTC20

Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs

Zygosity: Homozygous strain

fixedexpanded
Profile for YBR188C / NTC20

Click on Significant Values for Screen Details

Top fitness defect scores for YBR188C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_239	5hr heat shock (37°C) + mitomycin C	7.17	819.4 µM	1.66E-14	heat shock/prefoldin	8	3.82E-13
2	SGTC_241	5hr heat shock (37°C) + cantharidin	5.03	155.4 µM	5.12E-8	cell wall signaling	45	2.40E-7
3	SGTC_3204	9114183	4.89	49.5 µM	1.14E-7		12	4.92E-7
4	SGTC_314	1671-0008	4.73	2.7 µM	2.93E-7		27	1.14E-6
5	SGTC_364	0083-0097	4.00	67.7 µM	1.18E-5		22	3.14E-5
6	SGTC_290	2043-6722	3.83	48.1 µM	2.57E-5		11	6.32E-5
7	SGTC_238	5hr heat shock (37°C) + nsc-638432	3.76	8.9 µM	3.51E-5	heat shock/prefoldin	48	8.35E-5
8	SGTC_813	k007-0158	3.75	173.0 µM	3.77E-5	fatty acid desaturase (OLE1)	49	8.92E-5
9	SGTC_990	3985-0011	3.74	41.3 µM	3.87E-5	amide catabolism	29	9.11E-5
10	SGTC_1258	0671-0043	3.73	57.1 µM	4.12E-5		30	9.66E-5
11	SGTC_223	busulfan	3.69	5.0 mM	4.85E-5		11	1.12E-4
12	SGTC_2469	5655019	3.63	90.8 µM	6.32E-5		28	1.42E-4
13	SGTC_1251	0490-5699	3.50	265.0 µM	1.11E-4		54	2.36E-4
14	SGTC_1794	st048918	3.49	76.9 µM	1.16E-4		15	2.44E-4
15	SGTC_1145	3546-0597	3.48	10.2 µM	1.18E-4	mitochondrial processes	31	2.49E-4
16	SGTC_263	alizarin	3.39	107.9 µM	1.76E-4	iron homeostasis	88	3.55E-4
17	SGTC_431	k089-0097	3.38	107.0 µM	1.82E-4	iron homeostasis	69	3.66E-4
18	SGTC_2329	6138490	3.37	200.0 µM	1.83E-4		56	3.69E-4
19	SGTC_803	4488-1585	3.31	147.0 µM	2.42E-4		32	4.73E-4
20	SGTC_816	0325-0417	3.29	81.3 µM	2.62E-4		30	5.08E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.184	7.79E-27	YDR260C	SWM1	hom	Subunit of the anaphase-promoting complex, which is an E3 ubiquitin ligase that regulates the metaphase-anaphase transition and exit from mitosis; required for activation of the daughter-specific gene expression and spore wall maturation
0.183	1.26E-26	YLR027C	AAT2	hom	Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells
0.149	3.25E-18	YHR209W	CRG1	hom	S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin
0.148	5.14E-18	YCR063W	BUD31	hom	Component of the SF3b subcomplex of the U2 snRNP; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis
0.141	2.40E-16	YOR001W	RRP6	hom	Nuclear exosome exonuclease component; has 3'-5' exonuclease activity; involved in RNA processing, maturation, surveillance, degradation, tethering, and export; has similarity to E. coli RNase D and to human PM-Sc1 100 (EXOSC10); mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay
0.128	9.96E-14	YDR457W	TOM1	hom	E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase
0.111	9.65E-11	YLR200W	YKE2	hom	Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin
0.107	4.48E-10	YNL140C_p	YNL140C_p	hom	Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps THO2/YNL139C
0.107	5.48E-10	YKR024C	DBP7	hom	Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions
0.102	3.20E-9	YNL098C	RAS2	hom	GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes
0.100	6.36E-9	YPL017C	IRC15	hom	Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci
0.098	1.09E-8	YJR020W_d	YJR020W_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.097	1.82E-8	YHR041C	SRB2	hom	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance
0.096	2.83E-8	YJL171C	YJL171C	hom	GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress
0.090	1.64E-7	YKL007W	CAP1	hom	Alpha subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress