YBR195C / MSI1

Subunit of chromatin assembly factor I (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription; Msi1p localizes to both nucleus and cytoplasm and has an independent role as a negative regulator of the RAS/cAMP pathway via sequestration of Npr1p kinase

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR195C / MSI1

Click on Significant Values for Screen Details ID:SGTC_3|Compound:0833-0587|FD-Score:3.45|P-value:9.85E-5 ID:SGTC_103|Compound:0844-0104|FD-Score:4.90|P-value:6.10E-8 ID:SGTC_147|Compound:4466-0538|FD-Score:4.07|P-value:5.59E-6 ID:SGTC_203|Compound:k066-0004|FD-Score:4.04|P-value:6.72E-6 ID:SGTC_245|Compound:methotrexate|FD-Score:-2.85|P-value:9.94E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:3.03|P-value:5.39E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:3.07|P-value:4.78E-4 ID:SGTC_311|Compound:1315-0136|FD-Score:3.36|P-value:1.46E-4 ID:SGTC_345|Compound:1362-0200|FD-Score:-2.89|P-value:8.59E-4 ID:SGTC_532|Compound:1548-0774|FD-Score:4.06|P-value:6.15E-6 ID:SGTC_568|Compound:1193-0097|FD-Score:3.08|P-value:4.57E-4 ID:SGTC_697|Compound:0508-1955|FD-Score:3.70|P-value:3.32E-5 ID:SGTC_758|Compound:0868-0029|FD-Score:3.01|P-value:5.87E-4 ID:SGTC_888|Compound:0916-0002|FD-Score:-3.00|P-value:5.75E-4 ID:SGTC_930|Compound:2810-3379|FD-Score:4.10|P-value:4.88E-6 ID:SGTC_934|Compound:2950-0684|FD-Score:3.00|P-value:6.07E-4 ID:SGTC_1003|Compound:4049-0218|FD-Score:3.82|P-value:1.92E-5 ID:SGTC_1055|Compound:3448-0672|FD-Score:3.04|P-value:5.34E-4 ID:SGTC_1064|Compound:honokiol|FD-Score:3.28|P-value:2.05E-4 ID:SGTC_1202|Compound:1988-0068|FD-Score:3.55|P-value:6.50E-5 ID:SGTC_1307|Compound:1187-1599|FD-Score:3.56|P-value:6.33E-5 ID:SGTC_1364|Compound:1653-0028|FD-Score:3.38|P-value:1.33E-4 ID:SGTC_1381|Compound:2324-0066|FD-Score:-2.85|P-value:9.95E-4 ID:SGTC_1501|Compound:4491-0275|FD-Score:3.37|P-value:1.39E-4 ID:SGTC_1577|Compound:3(2'-chlorophenyl)-7-ethoxycoumarin|FD-Score:3.14|P-value:3.65E-4 ID:SGTC_1578|Compound:josamycin|FD-Score:-3.02|P-value:5.18E-4 ID:SGTC_1598|Compound:3(2'-chlorophenyl)-7-hydroxy-4-phenylcoumarin|FD-Score:5.01|P-value:3.18E-8 ID:SGTC_1600|Compound:coumarin 106|FD-Score:3.47|P-value:9.05E-5 ID:SGTC_1745|Compound:st081495|FD-Score:2.93|P-value:8.14E-4 ID:SGTC_1758|Compound:st042942|FD-Score:3.60|P-value:5.13E-5 ID:SGTC_1800|Compound:st050079|FD-Score:-3.00|P-value:5.54E-4 ID:SGTC_1826|Compound:st055655|FD-Score:3.12|P-value:3.87E-4 ID:SGTC_1879|Compound:gemcitabine|FD-Score:3.06|P-value:4.96E-4 ID:SGTC_1884|Compound:5633444|FD-Score:3.22|P-value:2.57E-4 ID:SGTC_1901|Compound:st060216|FD-Score:-3.04|P-value:4.88E-4 ID:SGTC_2096|Compound:5468907|FD-Score:4.21|P-value:2.85E-6 ID:SGTC_2354|Compound:9060492|FD-Score:3.20|P-value:2.88E-4 ID:SGTC_2366|Compound:9070441|FD-Score:4.44|P-value:8.38E-7 ID:SGTC_2407|Compound:paclitaxel|FD-Score:3.60|P-value:5.24E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:3.75|P-value:2.60E-5 ID:SGTC_2584|Compound:androsterone|FD-Score:3.45|P-value:1.02E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:3.74|P-value:2.77E-5 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:-3.49|P-value:7.70E-5 ID:SGTC_2780|Compound:5215298|FD-Score:2.97|P-value:6.98E-4 ID:SGTC_2782|Compound:7736369|FD-Score:5.00|P-value:3.40E-8 ID:SGTC_2817|Compound:7995342|FD-Score:3.12|P-value:3.89E-4 ID:SGTC_2887|Compound:9044784|FD-Score:3.18|P-value:3.11E-4 ID:SGTC_2921|Compound:7974240|FD-Score:3.44|P-value:1.04E-4 ID:SGTC_2925|Compound:9010536|FD-Score:-4.36|P-value:1.12E-6 ID:SGTC_2957|Compound:9076800|FD-Score:-2.96|P-value:6.60E-4 ID:SGTC_3037|Compound:9091026|FD-Score:-3.28|P-value:1.87E-4 ID:SGTC_3112|Compound:9123419|FD-Score:-3.29|P-value:1.78E-4 ID:SGTC_3148|Compound:9097893|FD-Score:3.00|P-value:6.25E-4 ID:SGTC_3311|Compound:9131146|FD-Score:4.14|P-value:3.97E-6 ID:SGTC_3|Compound:0833-0587|FD-Score:3.45|P-value:9.85E-5 ID:SGTC_103|Compound:0844-0104|FD-Score:4.90|P-value:6.10E-8 ID:SGTC_147|Compound:4466-0538|FD-Score:4.07|P-value:5.59E-6 ID:SGTC_203|Compound:k066-0004|FD-Score:4.04|P-value:6.72E-6 ID:SGTC_245|Compound:methotrexate|FD-Score:-2.85|P-value:9.94E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:3.03|P-value:5.39E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:3.07|P-value:4.78E-4 ID:SGTC_311|Compound:1315-0136|FD-Score:3.36|P-value:1.46E-4 ID:SGTC_345|Compound:1362-0200|FD-Score:-2.89|P-value:8.59E-4 ID:SGTC_532|Compound:1548-0774|FD-Score:4.06|P-value:6.15E-6 ID:SGTC_568|Compound:1193-0097|FD-Score:3.08|P-value:4.57E-4 ID:SGTC_697|Compound:0508-1955|FD-Score:3.70|P-value:3.32E-5 ID:SGTC_758|Compound:0868-0029|FD-Score:3.01|P-value:5.87E-4 ID:SGTC_888|Compound:0916-0002|FD-Score:-3.00|P-value:5.75E-4 ID:SGTC_930|Compound:2810-3379|FD-Score:4.10|P-value:4.88E-6 ID:SGTC_934|Compound:2950-0684|FD-Score:3.00|P-value:6.07E-4 ID:SGTC_1003|Compound:4049-0218|FD-Score:3.82|P-value:1.92E-5 ID:SGTC_1055|Compound:3448-0672|FD-Score:3.04|P-value:5.34E-4 ID:SGTC_1064|Compound:honokiol|FD-Score:3.28|P-value:2.05E-4 ID:SGTC_1202|Compound:1988-0068|FD-Score:3.55|P-value:6.50E-5 ID:SGTC_1307|Compound:1187-1599|FD-Score:3.56|P-value:6.33E-5 ID:SGTC_1364|Compound:1653-0028|FD-Score:3.38|P-value:1.33E-4 ID:SGTC_1381|Compound:2324-0066|FD-Score:-2.85|P-value:9.95E-4 ID:SGTC_1501|Compound:4491-0275|FD-Score:3.37|P-value:1.39E-4 ID:SGTC_1577|Compound:3(2'-chlorophenyl)-7-ethoxycoumarin|FD-Score:3.14|P-value:3.65E-4 ID:SGTC_1578|Compound:josamycin|FD-Score:-3.02|P-value:5.18E-4 ID:SGTC_1598|Compound:3(2'-chlorophenyl)-7-hydroxy-4-phenylcoumarin|FD-Score:5.01|P-value:3.18E-8 ID:SGTC_1600|Compound:coumarin 106|FD-Score:3.47|P-value:9.05E-5 ID:SGTC_1745|Compound:st081495|FD-Score:2.93|P-value:8.14E-4 ID:SGTC_1758|Compound:st042942|FD-Score:3.60|P-value:5.13E-5 ID:SGTC_1800|Compound:st050079|FD-Score:-3.00|P-value:5.54E-4 ID:SGTC_1826|Compound:st055655|FD-Score:3.12|P-value:3.87E-4 ID:SGTC_1879|Compound:gemcitabine|FD-Score:3.06|P-value:4.96E-4 ID:SGTC_1884|Compound:5633444|FD-Score:3.22|P-value:2.57E-4 ID:SGTC_1901|Compound:st060216|FD-Score:-3.04|P-value:4.88E-4 ID:SGTC_2096|Compound:5468907|FD-Score:4.21|P-value:2.85E-6 ID:SGTC_2354|Compound:9060492|FD-Score:3.20|P-value:2.88E-4 ID:SGTC_2366|Compound:9070441|FD-Score:4.44|P-value:8.38E-7 ID:SGTC_2407|Compound:paclitaxel|FD-Score:3.60|P-value:5.24E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:3.75|P-value:2.60E-5 ID:SGTC_2584|Compound:androsterone|FD-Score:3.45|P-value:1.02E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:3.74|P-value:2.77E-5 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:-3.49|P-value:7.70E-5 ID:SGTC_2780|Compound:5215298|FD-Score:2.97|P-value:6.98E-4 ID:SGTC_2782|Compound:7736369|FD-Score:5.00|P-value:3.40E-8 ID:SGTC_2817|Compound:7995342|FD-Score:3.12|P-value:3.89E-4 ID:SGTC_2887|Compound:9044784|FD-Score:3.18|P-value:3.11E-4 ID:SGTC_2921|Compound:7974240|FD-Score:3.44|P-value:1.04E-4 ID:SGTC_2925|Compound:9010536|FD-Score:-4.36|P-value:1.12E-6 ID:SGTC_2957|Compound:9076800|FD-Score:-2.96|P-value:6.60E-4 ID:SGTC_3037|Compound:9091026|FD-Score:-3.28|P-value:1.87E-4 ID:SGTC_3112|Compound:9123419|FD-Score:-3.29|P-value:1.78E-4 ID:SGTC_3148|Compound:9097893|FD-Score:3.00|P-value:6.25E-4 ID:SGTC_3311|Compound:9131146|FD-Score:4.14|P-value:3.97E-6

Top fitness defect scores for YBR195C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1598 3(2'-chlorophenyl)-7-hydroxy-4-phenylcoumarin 5.01 19.1 µM 3.18E-8 7 2.68E-7
2 SGTC_2782 7736369 5.00 71.4 µM 3.40E-8 17 2.83E-7
3 SGTC_103 0844-0104 4.90 51.8 µM 6.10E-8 ERAD & cell cycle 105 4.69E-7
4 SGTC_2366 9070441 4.44 60.6 µM 8.38E-7 20 4.49E-6
5 SGTC_2096 5468907 4.21 200.0 µM 2.85E-6 3 1.29E-5
6 SGTC_3311 9131146 4.14 2.9 µM 3.97E-6 cell wall 113 1.72E-5
7 SGTC_930 2810-3379 4.10 265.0 µM 4.88E-6 11 2.05E-5
8 SGTC_147 4466-0538 4.07 29.2 µM 5.59E-6 ergosterol biosynthesis 18 2.31E-5
9 SGTC_532 1548-0774 4.06 215.0 µM 6.15E-6 17 2.50E-5
10 SGTC_203 k066-0004 4.04 30.9 µM 6.72E-6 RSC & ERG11 18 2.71E-5
11 SGTC_1003 4049-0218 3.82 100.0 µM 1.92E-5 22 6.70E-5
12 SGTC_2582 agaric acid 3.75 100.0 µM 2.60E-5 redox potentiating 336 8.71E-5
13 SGTC_2651 1327-0036 3.74 5.0 µM 2.77E-5 179 9.19E-5
14 SGTC_697 0508-1955 3.70 564.0 µM 3.32E-5 47 1.08E-4
15 SGTC_1758 st042942 3.60 75.2 µM 5.13E-5 31 1.57E-4
16 SGTC_2407 paclitaxel 3.60 400.0 µM 5.24E-5 50 1.60E-4
17 SGTC_1307 1187-1599 3.56 12.2 µM 6.33E-5 PDR1 20 1.88E-4
18 SGTC_1202 1988-0068 3.55 29.6 µM 6.50E-5 RNA processing & uracil transport 41 1.93E-4
19 SGTC_1600 coumarin 106 3.47 71.1 µM 9.05E-5 41 2.56E-4
20 SGTC_3 0833-0587 3.45 95.6 µM 9.85E-5 redox potentiating 35 2.76E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.171 2.36E-23 YNL106C INP52 hom Polyphosphatidylinositol phosphatase, dephosphorylates a number of phosphatidylinositols (PIs) to PI; involved in endocytosis; hyperosmotic stress causes translocation to actin patches; synaptojanin-like protein with a Sac1 domain
0.161 6.58E-21 YML103C NUP188 hom Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC organization and nucleocytoplasmic transport; homologous to human NUP188
0.126 2.16E-13 YOR297C TIM18 hom Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; may mediate assembly or stability of the complex
0.125 3.97E-13 YIL129C TAO3 het Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with protein kinase Cbk1p and also with Kic1p
0.121 2.08E-12 YDL148C NOP14 het Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA
0.119 5.19E-12 YJR091C JSN1 hom Member of the Puf family of RNA-binding proteins, interacts with mRNAs encoding membrane-associated proteins; involved in localizing the Arp2/3 complex to mitochondria; overexpression causes increased sensitivity to benomyl
0.114 3.92E-11 YPL115C BEM3 hom Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly
0.113 4.71E-11 YPL101W ELP4 hom Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity
0.113 4.93E-11 YLL032C YLL032C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
0.112 6.46E-11 YOL003C PFA4 hom Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.112 7.22E-11 YJL019W MPS3 het Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p
0.111 9.71E-11 YMR070W MOT3 hom Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion
0.111 9.79E-11 YJR142W_p YJR142W_p hom Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member
0.109 2.15E-10 YOR195W SLK19 hom Kinetochore-associated protein required for normal segregation of chromosomes in meiosis and mitosis; component of the FEAR regulatory network, which promotes Cdc14p release from the nucleolus during anaphase; potential Cdc28p substrate
0.108 2.89E-10 YOL065C INP54 hom Phosphatidylinositol 4,5-bisphosphate 5-phosphatase with a role in secretion, localizes to the endoplasmic reticulum via the C-terminal tail; lacks the Sac1 domain and proline-rich region found in the other 3 INP proteins