YBR211C / AME1

Essential kinetochore protein associated with microtubules and SPBs; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-U and fission yeast Mis17; relative distribution to the nucleus increases upon DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YBR211C / AME1

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:4.99|P-value:3.92E-8 ID:SGTC_343|Compound:4130-2827|FD-Score:-3.15|P-value:4.41E-4 ID:SGTC_350|Compound:k064-0020|FD-Score:3.49|P-value:8.21E-5 ID:SGTC_361|Compound:000s-0510|FD-Score:2.86|P-value:9.99E-4 ID:SGTC_456|Compound:gingerol|FD-Score:-3.06|P-value:6.19E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:3.30|P-value:1.86E-4 ID:SGTC_1257|Compound:0669-0121|FD-Score:3.46|P-value:9.50E-5 ID:SGTC_1262|Compound:0737-0114|FD-Score:3.54|P-value:6.85E-5 ID:SGTC_1373|Compound:2887-0003|FD-Score:3.57|P-value:5.89E-5 ID:SGTC_1626|Compound:st003712|FD-Score:-3.67|P-value:5.17E-5 ID:SGTC_1628|Compound:st004252|FD-Score:3.40|P-value:1.23E-4 ID:SGTC_1771|Compound:st048882|FD-Score:-3.62|P-value:6.47E-5 ID:SGTC_1778|Compound:st046819|FD-Score:-3.22|P-value:3.38E-4 ID:SGTC_1933|Compound:st074711|FD-Score:3.38|P-value:1.30E-4 ID:SGTC_2002|Compound:4023503|FD-Score:3.64|P-value:4.25E-5 ID:SGTC_2008|Compound:4012253|FD-Score:-3.49|P-value:1.11E-4 ID:SGTC_2116|Compound:5511452|FD-Score:-2.93|P-value:9.97E-4 ID:SGTC_2244|Compound:6635614|FD-Score:3.44|P-value:1.03E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:-3.35|P-value:1.97E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:3.45|P-value:9.83E-5 ID:SGTC_2514|Compound:salinomycin|FD-Score:2.97|P-value:6.54E-4 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:-3.21|P-value:3.56E-4 ID:SGTC_2564|Compound:betulinic acid|FD-Score:5.36|P-value:4.21E-9 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:5.03|P-value:3.13E-8 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:3.96|P-value:1.00E-5 ID:SGTC_2686|Compound:alizarin|FD-Score:4.38|P-value:1.21E-6 ID:SGTC_2756|Compound:carbadox|FD-Score:2.93|P-value:7.79E-4 ID:SGTC_2873|Compound:9040596|FD-Score:3.60|P-value:5.10E-5 ID:SGTC_2874|Compound:9043904|FD-Score:-3.81|P-value:2.78E-5 ID:SGTC_2896|Compound:9052656|FD-Score:-3.75|P-value:3.62E-5 ID:SGTC_2905|Compound:7799065|FD-Score:3.29|P-value:1.91E-4 ID:SGTC_2957|Compound:9076800|FD-Score:3.23|P-value:2.39E-4 ID:SGTC_2966|Compound:9084041|FD-Score:3.06|P-value:4.76E-4 ID:SGTC_2981|Compound:9013952|FD-Score:2.93|P-value:7.59E-4 ID:SGTC_3050|Compound:9093513|FD-Score:-3.75|P-value:3.66E-5 ID:SGTC_3063|Compound:1327-0034|FD-Score:3.85|P-value:1.70E-5 ID:SGTC_3080|Compound:9117754|FD-Score:4.90|P-value:6.70E-8 ID:SGTC_3216|Compound:9129716|FD-Score:-3.13|P-value:4.70E-4 ID:SGTC_3218|Compound:9131285|FD-Score:-3.93|P-value:1.61E-5 ID:SGTC_3282|Compound:9108498|FD-Score:-3.64|P-value:6.03E-5 ID:SGTC_3283|Compound:9109332|FD-Score:-3.27|P-value:2.80E-4 ID:SGTC_3291|Compound:9121177|FD-Score:-3.01|P-value:7.33E-4 ID:SGTC_3296|Compound:9122258|FD-Score:3.87|P-value:1.52E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:4.99|P-value:3.92E-8 ID:SGTC_343|Compound:4130-2827|FD-Score:-3.15|P-value:4.41E-4 ID:SGTC_350|Compound:k064-0020|FD-Score:3.49|P-value:8.21E-5 ID:SGTC_361|Compound:000s-0510|FD-Score:2.86|P-value:9.99E-4 ID:SGTC_456|Compound:gingerol|FD-Score:-3.06|P-value:6.19E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:3.30|P-value:1.86E-4 ID:SGTC_1257|Compound:0669-0121|FD-Score:3.46|P-value:9.50E-5 ID:SGTC_1262|Compound:0737-0114|FD-Score:3.54|P-value:6.85E-5 ID:SGTC_1373|Compound:2887-0003|FD-Score:3.57|P-value:5.89E-5 ID:SGTC_1626|Compound:st003712|FD-Score:-3.67|P-value:5.17E-5 ID:SGTC_1628|Compound:st004252|FD-Score:3.40|P-value:1.23E-4 ID:SGTC_1771|Compound:st048882|FD-Score:-3.62|P-value:6.47E-5 ID:SGTC_1778|Compound:st046819|FD-Score:-3.22|P-value:3.38E-4 ID:SGTC_1933|Compound:st074711|FD-Score:3.38|P-value:1.30E-4 ID:SGTC_2002|Compound:4023503|FD-Score:3.64|P-value:4.25E-5 ID:SGTC_2008|Compound:4012253|FD-Score:-3.49|P-value:1.11E-4 ID:SGTC_2116|Compound:5511452|FD-Score:-2.93|P-value:9.97E-4 ID:SGTC_2244|Compound:6635614|FD-Score:3.44|P-value:1.03E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:-3.35|P-value:1.97E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:3.45|P-value:9.83E-5 ID:SGTC_2514|Compound:salinomycin|FD-Score:2.97|P-value:6.54E-4 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:-3.21|P-value:3.56E-4 ID:SGTC_2564|Compound:betulinic acid|FD-Score:5.36|P-value:4.21E-9 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:5.03|P-value:3.13E-8 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:3.96|P-value:1.00E-5 ID:SGTC_2686|Compound:alizarin|FD-Score:4.38|P-value:1.21E-6 ID:SGTC_2756|Compound:carbadox|FD-Score:2.93|P-value:7.79E-4 ID:SGTC_2873|Compound:9040596|FD-Score:3.60|P-value:5.10E-5 ID:SGTC_2874|Compound:9043904|FD-Score:-3.81|P-value:2.78E-5 ID:SGTC_2896|Compound:9052656|FD-Score:-3.75|P-value:3.62E-5 ID:SGTC_2905|Compound:7799065|FD-Score:3.29|P-value:1.91E-4 ID:SGTC_2957|Compound:9076800|FD-Score:3.23|P-value:2.39E-4 ID:SGTC_2966|Compound:9084041|FD-Score:3.06|P-value:4.76E-4 ID:SGTC_2981|Compound:9013952|FD-Score:2.93|P-value:7.59E-4 ID:SGTC_3050|Compound:9093513|FD-Score:-3.75|P-value:3.66E-5 ID:SGTC_3063|Compound:1327-0034|FD-Score:3.85|P-value:1.70E-5 ID:SGTC_3080|Compound:9117754|FD-Score:4.90|P-value:6.70E-8 ID:SGTC_3216|Compound:9129716|FD-Score:-3.13|P-value:4.70E-4 ID:SGTC_3218|Compound:9131285|FD-Score:-3.93|P-value:1.61E-5 ID:SGTC_3282|Compound:9108498|FD-Score:-3.64|P-value:6.03E-5 ID:SGTC_3283|Compound:9109332|FD-Score:-3.27|P-value:2.80E-4 ID:SGTC_3291|Compound:9121177|FD-Score:-3.01|P-value:7.33E-4 ID:SGTC_3296|Compound:9122258|FD-Score:3.87|P-value:1.52E-5

Top fitness defect scores for YBR211C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2564 betulinic acid 5.36 100.0 µM 4.21E-9 3 4.22E-8 0.24
2 SGTC_2567 aureobasidin a 5.03 60.0 nM 3.13E-8 24 2.43E-7 0.03
3 SGTC_58 0957-0393 4.99 9.0 µM 3.92E-8 cell wall 39 2.97E-7 0.04
4 SGTC_3080 9117754 4.90 49.5 µM 6.70E-8 2 4.74E-7 0.44
5 SGTC_2686 alizarin 4.38 95.4 µM 1.21E-6 RPP1 & pyrimidine depletion 6 5.94E-6 0.02
6 SGTC_2636 apigenin dimethyl ether 3.96 100.0 µM 1.00E-5 11 3.78E-5 0.10
7 SGTC_3296 9122258 3.87 64.0 µM 1.52E-5 redox potentiating 5 5.47E-5 0.01
8 SGTC_3063 1327-0034 3.85 4.0 µM 1.70E-5 10 6.02E-5 0.04
9 SGTC_2002 4023503 3.64 131.0 µM 4.25E-5 mitochondrial processes 12 1.34E-4 0.10
10 SGTC_2873 9040596 3.60 71.4 µM 5.10E-5 8 1.57E-4 8.00E-4
11 SGTC_1373 2887-0003 3.57 58.5 µM 5.89E-5 Golgi 15 1.78E-4 0.03
12 SGTC_1262 0737-0114 3.54 49.6 µM 6.85E-5 8 2.04E-4 0.07
13 SGTC_350 k064-0020 3.49 19.8 µM 8.21E-5 heme requiring 9 2.39E-4 0.13
14 SGTC_1257 0669-0121 3.46 16.0 µM 9.50E-5 4 2.71E-4 0.06
15 SGTC_2408 sulbentine 3.45 18.4 µM 9.83E-5 45 2.80E-4 0.01
16 SGTC_2244 6635614 3.44 200.0 µM 1.03E-4 3 2.91E-4 0.15
17 SGTC_1628 st004252 3.40 77.7 µM 1.23E-4 8 3.40E-4 0.00
18 SGTC_1933 st074711 3.38 15.9 µM 1.30E-4 17 3.56E-4 0.01
19 SGTC_682 0kpi-0099 3.30 17.0 µM 1.86E-4 52 4.88E-4 0.01
20 SGTC_2905 7799065 3.29 20.1 µM 1.91E-4 12 5.00E-4 0.01

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.205 4.75E-33 YKL004W AUR1 het Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance
0.179 1.53E-25 YDR382W RPP2B hom Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm
0.161 8.52E-21 YBR244W GPX2 hom Phospholipid hydroperoxide glutathione peroxidase; protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress; induced by glucose starvation; protein abundance increases in response to DNA replication stress
0.151 1.62E-18 YDR367W KEI1 het Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene
0.144 4.92E-17 YNL215W IES2 hom Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress
0.143 6.81E-17 YOR231W MKK1 hom MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication
0.142 1.61E-16 YBR120C CBP6 hom Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.141 2.56E-16 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.140 3.38E-16 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.135 3.52E-15 YJL150W_d YJL150W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.132 1.98E-14 YDL091C UBX3 hom Subunit of the DSC ubiquitin ligase complex; UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; ortholog of fission yeast Ucp10
0.130 4.84E-14 YGR058W PEF1 hom Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly
0.127 1.44E-13 YIL160C POT1 hom 3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids
0.124 4.50E-13 YKL115C_d YKL115C_d hom Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1
0.124 4.55E-13 YCR088W ABP1 hom Actin-binding protein of the cortical actin cytoskeleton, important for activation of the Arp2/3 complex that plays a key role actin in cytoskeleton organization; phosphorylation within its PRR (Proline-Rich Region), mediated by Cdc28p and Pho85p, protects Abp1p from proteolysis mediated by its own PEST sequences