YBR215W / HPC2

Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; mutants display synthetic defects with subunits of FACT, a complex that allows passage of RNA Pol II through nucleosomes

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR215W / HPC2

Click on Significant Values for Screen Details ID:SGTC_117|Compound:lucigenin|FD-Score:3.65|P-value:1.27E-4 ID:SGTC_198|Compound:3454-2873|FD-Score:5.80|P-value:3.17E-9 ID:SGTC_261|Compound:5-fluorouridine|FD-Score:3.32|P-value:4.41E-4 ID:SGTC_344|Compound:k064-0035|FD-Score:5.47|P-value:2.09E-8 ID:SGTC_348|Compound:k064-0012|FD-Score:3.28|P-value:5.02E-4 ID:SGTC_353|Compound:1988-1175|FD-Score:3.43|P-value:2.91E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-4.48|P-value:3.65E-6 ID:SGTC_541|Compound:4513-1321|FD-Score:-3.11|P-value:9.25E-4 ID:SGTC_566|Compound:0958-0068|FD-Score:-3.13|P-value:8.69E-4 ID:SGTC_609|Compound:0986-0026|FD-Score:3.11|P-value:9.13E-4 ID:SGTC_622|Compound:1348-1566|FD-Score:3.97|P-value:3.46E-5 ID:SGTC_635|Compound:k038-0028|FD-Score:3.47|P-value:2.58E-4 ID:SGTC_641|Compound:4092-0595|FD-Score:3.21|P-value:6.47E-4 ID:SGTC_757|Compound:1066-0008|FD-Score:-3.67|P-value:1.18E-4 ID:SGTC_779|Compound:1360-0071|FD-Score:3.84|P-value:5.93E-5 ID:SGTC_997|Compound:3996-0078|FD-Score:3.09|P-value:9.79E-4 ID:SGTC_1024|Compound:4507-0395|FD-Score:3.66|P-value:1.21E-4 ID:SGTC_1121|Compound:fusaric acid|FD-Score:-3.96|P-value:3.57E-5 ID:SGTC_1137|Compound:ixabepilone|FD-Score:-3.88|P-value:4.99E-5 ID:SGTC_1158|Compound:4092-0494|FD-Score:3.22|P-value:6.24E-4 ID:SGTC_1279|Compound:0874-0590|FD-Score:6.95|P-value:1.60E-12 ID:SGTC_1283|Compound:0922-0125|FD-Score:7.93|P-value:9.42E-16 ID:SGTC_1406|Compound:3909-7906|FD-Score:4.64|P-value:1.70E-6 ID:SGTC_1506|Compound:4226-2149|FD-Score:3.12|P-value:8.85E-4 ID:SGTC_1522|Compound:st012842|FD-Score:7.54|P-value:2.17E-14 ID:SGTC_1525|Compound:8-methoxypsoralen|FD-Score:6.93|P-value:1.86E-12 ID:SGTC_1627|Compound:st003713|FD-Score:3.39|P-value:3.42E-4 ID:SGTC_1716|Compound:st033144|FD-Score:-3.95|P-value:3.87E-5 ID:SGTC_1782|Compound:st039423|FD-Score:-3.11|P-value:9.33E-4 ID:SGTC_1855|Compound:st056195|FD-Score:4.41|P-value:4.96E-6 ID:SGTC_1942|Compound:st076330|FD-Score:4.89|P-value:4.75E-7 ID:SGTC_1948|Compound:st076329|FD-Score:5.42|P-value:2.82E-8 ID:SGTC_2058|Compound:5236698|FD-Score:-4.54|P-value:2.65E-6 ID:SGTC_2111|Compound:5486399|FD-Score:-3.37|P-value:3.68E-4 ID:SGTC_2391|Compound:6285546|FD-Score:3.70|P-value:1.03E-4 ID:SGTC_2393|Compound:9031399|FD-Score:3.46|P-value:2.60E-4 ID:SGTC_2524|Compound:avocatin b|FD-Score:3.12|P-value:8.97E-4 ID:SGTC_2683|Compound:piperine|FD-Score:-3.70|P-value:1.06E-4 ID:SGTC_2800|Compound:7887935|FD-Score:3.56|P-value:1.77E-4 ID:SGTC_3004|Compound:9076122|FD-Score:4.62|P-value:1.85E-6 ID:SGTC_3059|Compound:1327-0034|FD-Score:3.81|P-value:6.85E-5 ID:SGTC_3307|Compound:9126885|FD-Score:3.21|P-value:6.44E-4 ID:SGTC_117|Compound:lucigenin|FD-Score:3.65|P-value:1.27E-4 ID:SGTC_198|Compound:3454-2873|FD-Score:5.80|P-value:3.17E-9 ID:SGTC_261|Compound:5-fluorouridine|FD-Score:3.32|P-value:4.41E-4 ID:SGTC_344|Compound:k064-0035|FD-Score:5.47|P-value:2.09E-8 ID:SGTC_348|Compound:k064-0012|FD-Score:3.28|P-value:5.02E-4 ID:SGTC_353|Compound:1988-1175|FD-Score:3.43|P-value:2.91E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-4.48|P-value:3.65E-6 ID:SGTC_541|Compound:4513-1321|FD-Score:-3.11|P-value:9.25E-4 ID:SGTC_566|Compound:0958-0068|FD-Score:-3.13|P-value:8.69E-4 ID:SGTC_609|Compound:0986-0026|FD-Score:3.11|P-value:9.13E-4 ID:SGTC_622|Compound:1348-1566|FD-Score:3.97|P-value:3.46E-5 ID:SGTC_635|Compound:k038-0028|FD-Score:3.47|P-value:2.58E-4 ID:SGTC_641|Compound:4092-0595|FD-Score:3.21|P-value:6.47E-4 ID:SGTC_757|Compound:1066-0008|FD-Score:-3.67|P-value:1.18E-4 ID:SGTC_779|Compound:1360-0071|FD-Score:3.84|P-value:5.93E-5 ID:SGTC_997|Compound:3996-0078|FD-Score:3.09|P-value:9.79E-4 ID:SGTC_1024|Compound:4507-0395|FD-Score:3.66|P-value:1.21E-4 ID:SGTC_1121|Compound:fusaric acid|FD-Score:-3.96|P-value:3.57E-5 ID:SGTC_1137|Compound:ixabepilone|FD-Score:-3.88|P-value:4.99E-5 ID:SGTC_1158|Compound:4092-0494|FD-Score:3.22|P-value:6.24E-4 ID:SGTC_1279|Compound:0874-0590|FD-Score:6.95|P-value:1.60E-12 ID:SGTC_1283|Compound:0922-0125|FD-Score:7.93|P-value:9.42E-16 ID:SGTC_1406|Compound:3909-7906|FD-Score:4.64|P-value:1.70E-6 ID:SGTC_1506|Compound:4226-2149|FD-Score:3.12|P-value:8.85E-4 ID:SGTC_1522|Compound:st012842|FD-Score:7.54|P-value:2.17E-14 ID:SGTC_1525|Compound:8-methoxypsoralen|FD-Score:6.93|P-value:1.86E-12 ID:SGTC_1627|Compound:st003713|FD-Score:3.39|P-value:3.42E-4 ID:SGTC_1716|Compound:st033144|FD-Score:-3.95|P-value:3.87E-5 ID:SGTC_1782|Compound:st039423|FD-Score:-3.11|P-value:9.33E-4 ID:SGTC_1855|Compound:st056195|FD-Score:4.41|P-value:4.96E-6 ID:SGTC_1942|Compound:st076330|FD-Score:4.89|P-value:4.75E-7 ID:SGTC_1948|Compound:st076329|FD-Score:5.42|P-value:2.82E-8 ID:SGTC_2058|Compound:5236698|FD-Score:-4.54|P-value:2.65E-6 ID:SGTC_2111|Compound:5486399|FD-Score:-3.37|P-value:3.68E-4 ID:SGTC_2391|Compound:6285546|FD-Score:3.70|P-value:1.03E-4 ID:SGTC_2393|Compound:9031399|FD-Score:3.46|P-value:2.60E-4 ID:SGTC_2524|Compound:avocatin b|FD-Score:3.12|P-value:8.97E-4 ID:SGTC_2683|Compound:piperine|FD-Score:-3.70|P-value:1.06E-4 ID:SGTC_2800|Compound:7887935|FD-Score:3.56|P-value:1.77E-4 ID:SGTC_3004|Compound:9076122|FD-Score:4.62|P-value:1.85E-6 ID:SGTC_3059|Compound:1327-0034|FD-Score:3.81|P-value:6.85E-5 ID:SGTC_3307|Compound:9126885|FD-Score:3.21|P-value:6.44E-4

Top fitness defect scores for YBR215W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1283 0922-0125 7.93 16.4 µM 9.42E-16 1 1.07E-15
2 SGTC_1522 st012842 7.54 45.6 µM 2.17E-14 RNA pol III & RNase P/MRP 3 2.44E-14
3 SGTC_1279 0874-0590 6.95 65.2 µM 1.60E-12 3 1.77E-12
4 SGTC_1525 8-methoxypsoralen 6.93 92.5 µM 1.86E-12 DNA damage response 6 2.06E-12
5 SGTC_198 3454-2873 5.80 16.2 µM 3.17E-9 60S ribosome export 2 3.40E-9
6 SGTC_344 k064-0035 5.47 6.4 µM 2.09E-8 60S ribosome export 5 2.23E-8
7 SGTC_1948 st076329 5.42 66.6 µM 2.82E-8 8 3.00E-8
8 SGTC_1942 st076330 4.89 65.2 µM 4.75E-7 11 5.01E-7
9 SGTC_1406 3909-7906 4.64 11.9 µM 1.70E-6 60S ribosome export 4 1.78E-6
10 SGTC_3004 9076122 4.62 71.4 µM 1.85E-6 8 1.94E-6
11 SGTC_1855 st056195 4.41 39.6 µM 4.96E-6 60S ribosome export 10 5.18E-6
12 SGTC_622 1348-1566 3.97 26.3 µM 3.46E-5 19 3.59E-5
13 SGTC_779 1360-0071 3.84 233.0 µM 5.93E-5 fatty acid desaturase (OLE1) 29 6.13E-5
14 SGTC_3059 1327-0034 3.81 3.0 µM 6.85E-5 58 7.08E-5
15 SGTC_2391 6285546 3.70 200.0 µM 1.03E-4 24 1.06E-4
16 SGTC_1024 4507-0395 3.66 26.0 µM 1.21E-4 TSC3-RPN4 34 1.24E-4
17 SGTC_117 lucigenin 3.65 22.1 µM 1.27E-4 redox potentiating 101 1.31E-4
18 SGTC_2800 7887935 3.56 81.8 µM 1.77E-4 RPP1 & pyrimidine depletion 47 1.83E-4
19 SGTC_635 k038-0028 3.47 24.7 µM 2.58E-4 ubiquinone biosynthesis & proteasome 62 2.65E-4
20 SGTC_2393 9031399 3.46 185.9 µM 2.60E-4 RNA processing & uracil transport 49 2.68E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.129 5.38E-14 YGL179C TOS3 hom Protein kinase, related to and functionally redundant with Elm1p and Sak1p for the phosphorylation and activation of Snf1p; functionally orthologous to LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome
0.110 1.84E-10 YLL026W HSP104 hom Disaggregase; heat shock protein that cooperates with Ydj1p (Hsp40) and Ssa1p (Hsp70) to refold and reactivate previously denatured, aggregated proteins; responsive to stresses including: heat, ethanol, and sodium arsenite; involved in [PSI+] propagation; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress
0.105 9.83E-10 YPR020W ATP20 hom Subunit g of the mitochondrial F1F0 ATP synthase; reversibly phosphorylated on two residues; unphosphorylated form is required for dimerization of the ATP synthase complex
0.102 3.39E-9 YJR005C-A_p YJR005C-A_p hom Putative protein of unknown function; originally identified as a syntenic homolog of an Ashbya gossypii gene; YJR005C-A has a paralog, YGR169C-A, that arose from the whole genome duplication
0.099 7.83E-9 YOR116C RPO31 het RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
0.091 1.22E-7 YBR115C LYS2 hom Alpha aminoadipate reductase, catalyzes the reduction of alpha-aminoadipate to alpha-aminoadipate 6-semialdehyde, which is the fifth step in biosynthesis of lysine; activation requires posttranslational phosphopantetheinylation by Lys5p
0.086 5.69E-7 YAL044W-A_p YAL044W-A_p hom Putative protein of unknown function; similar to S. pombe uvi31 which is a putative DNA repair protein
0.081 2.36E-6 YBR221W-A_p YBR221W-A_p hom Putative protein of unknown function; identified by expression profiling and mass spectrometry
0.080 3.34E-6 YMR149W SWP1 het Delta subunit of the oligosaccharyl transferase glycoprotein complex, which is required for N-linked glycosylation of proteins in the endoplasmic reticulum
0.080 3.60E-6 YGL099W LSG1 het Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
0.080 3.88E-6 YOR136W IDH2 hom Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated
0.078 6.16E-6 YLR429W CRN1 hom Coronin, cortical actin cytoskeletal component that associates with the Arp2p/Arp3p complex to regulate its activity; plays a role in regulation of actin patch assembly
0.076 9.24E-6 YDL036C PUS9 hom Mitochondrial tRNA:pseudouridine synthase, catalyzes the formation of pseudouridine at position 32 in mitochondrial tRNAs; contains an N-terminal mitochondrial targeting sequence
0.076 9.39E-6 YNL146W_p YNL146W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNL146W is not an essential gene
0.076 1.05E-5 YMR298W LIP1 het Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis