YBR218C / PYC2

Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR218C / PYC2

Click on Significant Values for Screen Details ID:SGTC_3|Compound:0833-0587|FD-Score:4.26|P-value:2.42E-5 ID:SGTC_213|Compound:1246-0773|FD-Score:6.09|P-value:3.15E-9 ID:SGTC_577|Compound:r071-0003|FD-Score:3.95|P-value:8.38E-5 ID:SGTC_783|Compound:0848-0141|FD-Score:-3.59|P-value:2.82E-4 ID:SGTC_868|Compound:0527-0199|FD-Score:-3.49|P-value:4.05E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:4.01|P-value:6.66E-5 ID:SGTC_1158|Compound:4092-0494|FD-Score:3.27|P-value:9.23E-4 ID:SGTC_1477|Compound:0139-0234|FD-Score:-3.68|P-value:2.04E-4 ID:SGTC_1562|Compound:morin hydrate|FD-Score:3.39|P-value:6.10E-4 ID:SGTC_1673|Compound:st016611|FD-Score:-5.30|P-value:1.90E-7 ID:SGTC_1679|Compound:4130-1276|FD-Score:5.48|P-value:8.27E-8 ID:SGTC_1795|Compound:st049545|FD-Score:3.51|P-value:4.11E-4 ID:SGTC_1843|Compound:st055809|FD-Score:3.60|P-value:2.98E-4 ID:SGTC_1887|Compound:st057648|FD-Score:-3.30|P-value:7.71E-4 ID:SGTC_1942|Compound:st076330|FD-Score:5.56|P-value:5.52E-8 ID:SGTC_1964|Compound:st076914|FD-Score:4.82|P-value:2.13E-6 ID:SGTC_2085|Compound:5307070|FD-Score:3.97|P-value:7.78E-5 ID:SGTC_2086|Compound:5274770|FD-Score:-3.26|P-value:8.74E-4 ID:SGTC_2101|Compound:5377231|FD-Score:5.16|P-value:4.33E-7 ID:SGTC_2106|Compound:5357045|FD-Score:3.52|P-value:3.99E-4 ID:SGTC_2157|Compound:5649910|FD-Score:-3.69|P-value:2.00E-4 ID:SGTC_2160|Compound:5660466|FD-Score:4.47|P-value:1.01E-5 ID:SGTC_2326|Compound:6164455|FD-Score:3.25|P-value:9.83E-4 ID:SGTC_2506|Compound:ethyl hematommate|FD-Score:3.32|P-value:7.89E-4 ID:SGTC_2542|Compound:2-hydroxy-5 (6)epoxy-tetrahydrocaryophyllene|FD-Score:-5.14|P-value:4.15E-7 ID:SGTC_2543|Compound:pimpinellin|FD-Score:3.81|P-value:1.41E-4 ID:SGTC_2558|Compound:carylophyllene oxide|FD-Score:5.01|P-value:8.56E-7 ID:SGTC_2559|Compound:tetrahydrosappanone a trimethyl ether|FD-Score:3.86|P-value:1.16E-4 ID:SGTC_2634|Compound:4'-methoxyflavone|FD-Score:3.95|P-value:8.33E-5 ID:SGTC_2635|Compound:hydroquinidine|FD-Score:-5.00|P-value:8.29E-7 ID:SGTC_2639|Compound:lactacystin|FD-Score:5.31|P-value:2.06E-7 ID:SGTC_2654|Compound:protoveratrine a|FD-Score:3.94|P-value:8.48E-5 ID:SGTC_2663|Compound:amphotericin b|FD-Score:4.78|P-value:2.52E-6 ID:SGTC_2779|Compound:methotrexate|FD-Score:-3.43|P-value:4.93E-4 ID:SGTC_2783|Compound:7738165|FD-Score:3.77|P-value:1.65E-4 ID:SGTC_2790|Compound:5423970|FD-Score:5.40|P-value:1.29E-7 ID:SGTC_2945|Compound:9067681|FD-Score:3.28|P-value:8.79E-4 ID:SGTC_2949|Compound:9072542|FD-Score:-3.57|P-value:3.10E-4 ID:SGTC_2951|Compound:9072950|FD-Score:5.75|P-value:2.06E-8 ID:SGTC_2967|Compound:9089538|FD-Score:-3.94|P-value:7.91E-5 ID:SGTC_3112|Compound:9123419|FD-Score:3.53|P-value:3.81E-4 ID:SGTC_3130|Compound:9127414|FD-Score:4.15|P-value:3.82E-5 ID:SGTC_3131|Compound:9127911|FD-Score:4.04|P-value:5.88E-5 ID:SGTC_3135|Compound:9127742|FD-Score:4.45|P-value:1.11E-5 ID:SGTC_3277|Compound:9138724|FD-Score:-3.22|P-value:9.92E-4 ID:SGTC_3281|Compound:9140613|FD-Score:4.98|P-value:9.99E-7 ID:SGTC_3|Compound:0833-0587|FD-Score:4.26|P-value:2.42E-5 ID:SGTC_213|Compound:1246-0773|FD-Score:6.09|P-value:3.15E-9 ID:SGTC_577|Compound:r071-0003|FD-Score:3.95|P-value:8.38E-5 ID:SGTC_783|Compound:0848-0141|FD-Score:-3.59|P-value:2.82E-4 ID:SGTC_868|Compound:0527-0199|FD-Score:-3.49|P-value:4.05E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:4.01|P-value:6.66E-5 ID:SGTC_1158|Compound:4092-0494|FD-Score:3.27|P-value:9.23E-4 ID:SGTC_1477|Compound:0139-0234|FD-Score:-3.68|P-value:2.04E-4 ID:SGTC_1562|Compound:morin hydrate|FD-Score:3.39|P-value:6.10E-4 ID:SGTC_1673|Compound:st016611|FD-Score:-5.30|P-value:1.90E-7 ID:SGTC_1679|Compound:4130-1276|FD-Score:5.48|P-value:8.27E-8 ID:SGTC_1795|Compound:st049545|FD-Score:3.51|P-value:4.11E-4 ID:SGTC_1843|Compound:st055809|FD-Score:3.60|P-value:2.98E-4 ID:SGTC_1887|Compound:st057648|FD-Score:-3.30|P-value:7.71E-4 ID:SGTC_1942|Compound:st076330|FD-Score:5.56|P-value:5.52E-8 ID:SGTC_1964|Compound:st076914|FD-Score:4.82|P-value:2.13E-6 ID:SGTC_2085|Compound:5307070|FD-Score:3.97|P-value:7.78E-5 ID:SGTC_2086|Compound:5274770|FD-Score:-3.26|P-value:8.74E-4 ID:SGTC_2101|Compound:5377231|FD-Score:5.16|P-value:4.33E-7 ID:SGTC_2106|Compound:5357045|FD-Score:3.52|P-value:3.99E-4 ID:SGTC_2157|Compound:5649910|FD-Score:-3.69|P-value:2.00E-4 ID:SGTC_2160|Compound:5660466|FD-Score:4.47|P-value:1.01E-5 ID:SGTC_2326|Compound:6164455|FD-Score:3.25|P-value:9.83E-4 ID:SGTC_2506|Compound:ethyl hematommate|FD-Score:3.32|P-value:7.89E-4 ID:SGTC_2542|Compound:2-hydroxy-5 (6)epoxy-tetrahydrocaryophyllene|FD-Score:-5.14|P-value:4.15E-7 ID:SGTC_2543|Compound:pimpinellin|FD-Score:3.81|P-value:1.41E-4 ID:SGTC_2558|Compound:carylophyllene oxide|FD-Score:5.01|P-value:8.56E-7 ID:SGTC_2559|Compound:tetrahydrosappanone a trimethyl ether|FD-Score:3.86|P-value:1.16E-4 ID:SGTC_2634|Compound:4'-methoxyflavone|FD-Score:3.95|P-value:8.33E-5 ID:SGTC_2635|Compound:hydroquinidine|FD-Score:-5.00|P-value:8.29E-7 ID:SGTC_2639|Compound:lactacystin|FD-Score:5.31|P-value:2.06E-7 ID:SGTC_2654|Compound:protoveratrine a|FD-Score:3.94|P-value:8.48E-5 ID:SGTC_2663|Compound:amphotericin b|FD-Score:4.78|P-value:2.52E-6 ID:SGTC_2779|Compound:methotrexate|FD-Score:-3.43|P-value:4.93E-4 ID:SGTC_2783|Compound:7738165|FD-Score:3.77|P-value:1.65E-4 ID:SGTC_2790|Compound:5423970|FD-Score:5.40|P-value:1.29E-7 ID:SGTC_2945|Compound:9067681|FD-Score:3.28|P-value:8.79E-4 ID:SGTC_2949|Compound:9072542|FD-Score:-3.57|P-value:3.10E-4 ID:SGTC_2951|Compound:9072950|FD-Score:5.75|P-value:2.06E-8 ID:SGTC_2967|Compound:9089538|FD-Score:-3.94|P-value:7.91E-5 ID:SGTC_3112|Compound:9123419|FD-Score:3.53|P-value:3.81E-4 ID:SGTC_3130|Compound:9127414|FD-Score:4.15|P-value:3.82E-5 ID:SGTC_3131|Compound:9127911|FD-Score:4.04|P-value:5.88E-5 ID:SGTC_3135|Compound:9127742|FD-Score:4.45|P-value:1.11E-5 ID:SGTC_3277|Compound:9138724|FD-Score:-3.22|P-value:9.92E-4 ID:SGTC_3281|Compound:9140613|FD-Score:4.98|P-value:9.99E-7

Top fitness defect scores for YBR218C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_213 1246-0773 6.09 967.0 nM 3.15E-9 5 5.81E-10
2 SGTC_2951 9072950 5.75 58.1 µM 2.06E-8 ERG2 7 4.52E-9
3 SGTC_1942 st076330 5.56 65.2 µM 5.52E-8 6 1.33E-8
4 SGTC_1679 4130-1276 5.48 6.7 µM 8.27E-8 Golgi 31 2.07E-8
5 SGTC_2790 5423970 5.40 71.4 µM 1.29E-7 endomembrane recycling 29 3.35E-8
6 SGTC_2639 lactacystin 5.31 53.0 µM 2.06E-7 10 5.62E-8
7 SGTC_2101 5377231 5.16 179.2 µM 4.33E-7 2 1.26E-7
8 SGTC_2558 carylophyllene oxide 5.01 100.0 µM 8.56E-7 2 2.66E-7
9 SGTC_3281 9140613 4.98 49.5 µM 9.99E-7 RPP1 & pyrimidine depletion 8 3.15E-7
10 SGTC_1964 st076914 4.82 61.0 µM 2.13E-6 RPP1 & pyrimidine depletion 6 7.17E-7
11 SGTC_2663 amphotericin b 4.78 100.0 µM 2.52E-6 6 8.63E-7
12 SGTC_2160 5660466 4.47 132.9 µM 1.01E-5 60S ribosome export 7 3.95E-6
13 SGTC_3135 9127742 4.45 49.5 µM 1.11E-5 13 4.34E-6
14 SGTC_3 0833-0587 4.26 95.6 µM 2.42E-5 redox potentiating 16 1.02E-5
15 SGTC_3130 9127414 4.15 49.5 µM 3.82E-5 17 1.68E-5
16 SGTC_3131 9127911 4.04 49.5 µM 5.88E-5 28 2.69E-5
17 SGTC_1083 didemethylchlorpromazine sulfoxide 4.01 707.4 nM 6.66E-5 redox potentiating 80 3.08E-5
18 SGTC_2085 5307070 3.97 144.1 µM 7.78E-5 superoxide 28 3.65E-5
19 SGTC_2634 4'-methoxyflavone 3.95 100.0 µM 8.33E-5 33 3.94E-5
20 SGTC_577 r071-0003 3.95 38.6 µM 8.38E-5 fatty acid desaturase (OLE1) 54 3.96E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.118 5.96E-12 YMR079W SEC14 het Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs
0.117 1.12E-11 YPL251W_d YPL251W_d het Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C
0.107 6.04E-10 YBR250W SPO23 hom Protein of unknown function; associates with meiosis-specific protein Spo1p
0.104 1.40E-9 YNL285W_d YNL285W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.096 2.71E-8 YOL091W SPO21 hom Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation
0.090 2.05E-7 YEL052W AFG1 hom Conserved protein that may act as a chaperone in the degradation of misfolded or unassembled cytochrome c oxidase subunits; localized to matrix face of the mitochondrial inner membrane; member of the AAA family but lacks a protease domain
0.089 2.67E-7 YPL252C YAH1 het Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin
0.088 3.76E-7 YJL064W_d YJL064W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YJL065C/DLS1
0.083 1.64E-6 YDR431W_d YDR431W_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.081 2.44E-6 YER045C ACA1 hom ATF/CREB family basic leucine zipper (bZIP) transcription factor; binds as a homodimer to the ATF/CREB consensus sequence TGACGTCA; important for carbon source utilization; target genes include GRE2 and COS8; ACA1 has a paralog, CST6, that arose from the whole genome duplication
0.079 4.44E-6 YLR081W GAL2 hom Galactose permease, required for utilization of galactose; also able to transport glucose
0.073 2.46E-5 YKL196C YKT6 het Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus
0.072 2.83E-5 YBR300C_d YBR300C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR301W; YBR300C is not an essential gene
0.072 2.93E-5 YLR166C SEC10 het Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion
0.072 3.22E-5 YDL133W SRF1 hom Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients