YBR221C / PDB1

E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved multi-protein complex found in mitochondria

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR221C / PDB1

Click on Significant Values for Screen Details ID:SGTC_7|Compound:0192-1582|FD-Score:13.00|P-value:1.41E-42 ID:SGTC_34|Compound:0469-0683|FD-Score:6.73|P-value:8.14E-13 ID:SGTC_46|Compound:0329-0187a|FD-Score:4.26|P-value:3.55E-6 ID:SGTC_54|Compound:0159-0055|FD-Score:3.07|P-value:5.89E-4 ID:SGTC_59|Compound:0330-0160|FD-Score:4.20|P-value:4.90E-6 ID:SGTC_73|Compound:0327-0325|FD-Score:4.52|P-value:9.70E-7 ID:SGTC_90|Compound:0870-0863|FD-Score:4.74|P-value:3.04E-7 ID:SGTC_163|Compound:1327-0036|FD-Score:3.42|P-value:1.54E-4 ID:SGTC_278|Compound:4092-0821|FD-Score:-3.04|P-value:9.05E-4 ID:SGTC_279|Compound:1273-0055|FD-Score:3.34|P-value:2.14E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:2.95|P-value:8.99E-4 ID:SGTC_327|Compound:1273-0057|FD-Score:3.51|P-value:1.05E-4 ID:SGTC_331|Compound:k048-0133|FD-Score:3.51|P-value:1.04E-4 ID:SGTC_350|Compound:k064-0020|FD-Score:3.36|P-value:1.93E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.22|P-value:3.40E-4 ID:SGTC_455|Compound:paf C-16|FD-Score:3.01|P-value:7.30E-4 ID:SGTC_537|Compound:1334-0398|FD-Score:2.95|P-value:9.11E-4 ID:SGTC_628|Compound:1636-0253|FD-Score:3.04|P-value:6.50E-4 ID:SGTC_668|Compound:1682-0499|FD-Score:3.13|P-value:4.73E-4 ID:SGTC_769|Compound:0302-0231|FD-Score:3.00|P-value:7.63E-4 ID:SGTC_773|Compound:4358-1457|FD-Score:4.51|P-value:1.01E-6 ID:SGTC_824|Compound:1304-0008|FD-Score:3.18|P-value:3.85E-4 ID:SGTC_833|Compound:3852-0053|FD-Score:3.94|P-value:1.63E-5 ID:SGTC_937|Compound:3122-0005|FD-Score:3.43|P-value:1.45E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:3.25|P-value:3.02E-4 ID:SGTC_1132|Compound:3456-3614|FD-Score:3.64|P-value:6.21E-5 ID:SGTC_1190|Compound:1486-1332|FD-Score:3.35|P-value:2.02E-4 ID:SGTC_1346|Compound:1488-2224|FD-Score:4.15|P-value:6.20E-6 ID:SGTC_1359|Compound:1582-0056|FD-Score:3.91|P-value:1.86E-5 ID:SGTC_1365|Compound:1672-3266|FD-Score:4.55|P-value:8.53E-7 ID:SGTC_1530|Compound:rhein|FD-Score:3.54|P-value:9.27E-5 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:-3.44|P-value:2.05E-4 ID:SGTC_1635|Compound:st007035|FD-Score:3.69|P-value:4.89E-5 ID:SGTC_1712|Compound:st032288|FD-Score:-3.93|P-value:2.59E-5 ID:SGTC_1727|Compound:st034309|FD-Score:-5.06|P-value:8.56E-8 ID:SGTC_1754|Compound:st042065|FD-Score:-3.54|P-value:1.36E-4 ID:SGTC_1762|Compound:st045503|FD-Score:-3.31|P-value:3.35E-4 ID:SGTC_1786|Compound:berberine|FD-Score:3.99|P-value:1.32E-5 ID:SGTC_1800|Compound:st050079|FD-Score:-4.19|P-value:7.75E-6 ID:SGTC_1806|Compound:st050005|FD-Score:-3.09|P-value:7.47E-4 ID:SGTC_1818|Compound:st052136|FD-Score:-4.28|P-value:5.14E-6 ID:SGTC_1862|Compound:st057513|FD-Score:3.24|P-value:3.10E-4 ID:SGTC_1913|Compound:st060213|FD-Score:3.53|P-value:9.74E-5 ID:SGTC_1972|Compound:st070201|FD-Score:-3.55|P-value:1.28E-4 ID:SGTC_2021|Compound:4019055|FD-Score:-3.28|P-value:3.78E-4 ID:SGTC_2202|Compound:7226349|FD-Score:-3.19|P-value:5.27E-4 ID:SGTC_2264|Compound:7948757|FD-Score:-3.53|P-value:1.39E-4 ID:SGTC_2278|Compound:7948257|FD-Score:3.69|P-value:5.07E-5 ID:SGTC_2338|Compound:7999974|FD-Score:-5.86|P-value:6.99E-10 ID:SGTC_2340|Compound:7987427|FD-Score:3.75|P-value:3.78E-5 ID:SGTC_2341|Compound:7987486|FD-Score:3.21|P-value:3.53E-4 ID:SGTC_2350|Compound:9023895|FD-Score:3.89|P-value:2.07E-5 ID:SGTC_2570|Compound:licareol|FD-Score:3.94|P-value:1.64E-5 ID:SGTC_2766|Compound:oltipraz|FD-Score:2.94|P-value:9.27E-4 ID:SGTC_3012|Compound:9080433|FD-Score:3.41|P-value:1.60E-4 ID:SGTC_3299|Compound:9121982|FD-Score:9.49|P-value:1.45E-23 ID:SGTC_3326|Compound:9140064|FD-Score:-3.15|P-value:6.13E-4 ID:SGTC_3347|Compound:9188721|FD-Score:3.64|P-value:6.11E-5 ID:SGTC_3354|Compound:9151612|FD-Score:3.42|P-value:1.50E-4 ID:SGTC_7|Compound:0192-1582|FD-Score:13.00|P-value:1.41E-42 ID:SGTC_34|Compound:0469-0683|FD-Score:6.73|P-value:8.14E-13 ID:SGTC_46|Compound:0329-0187a|FD-Score:4.26|P-value:3.55E-6 ID:SGTC_54|Compound:0159-0055|FD-Score:3.07|P-value:5.89E-4 ID:SGTC_59|Compound:0330-0160|FD-Score:4.20|P-value:4.90E-6 ID:SGTC_73|Compound:0327-0325|FD-Score:4.52|P-value:9.70E-7 ID:SGTC_90|Compound:0870-0863|FD-Score:4.74|P-value:3.04E-7 ID:SGTC_163|Compound:1327-0036|FD-Score:3.42|P-value:1.54E-4 ID:SGTC_278|Compound:4092-0821|FD-Score:-3.04|P-value:9.05E-4 ID:SGTC_279|Compound:1273-0055|FD-Score:3.34|P-value:2.14E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:2.95|P-value:8.99E-4 ID:SGTC_327|Compound:1273-0057|FD-Score:3.51|P-value:1.05E-4 ID:SGTC_331|Compound:k048-0133|FD-Score:3.51|P-value:1.04E-4 ID:SGTC_350|Compound:k064-0020|FD-Score:3.36|P-value:1.93E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.22|P-value:3.40E-4 ID:SGTC_455|Compound:paf C-16|FD-Score:3.01|P-value:7.30E-4 ID:SGTC_537|Compound:1334-0398|FD-Score:2.95|P-value:9.11E-4 ID:SGTC_628|Compound:1636-0253|FD-Score:3.04|P-value:6.50E-4 ID:SGTC_668|Compound:1682-0499|FD-Score:3.13|P-value:4.73E-4 ID:SGTC_769|Compound:0302-0231|FD-Score:3.00|P-value:7.63E-4 ID:SGTC_773|Compound:4358-1457|FD-Score:4.51|P-value:1.01E-6 ID:SGTC_824|Compound:1304-0008|FD-Score:3.18|P-value:3.85E-4 ID:SGTC_833|Compound:3852-0053|FD-Score:3.94|P-value:1.63E-5 ID:SGTC_937|Compound:3122-0005|FD-Score:3.43|P-value:1.45E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:3.25|P-value:3.02E-4 ID:SGTC_1132|Compound:3456-3614|FD-Score:3.64|P-value:6.21E-5 ID:SGTC_1190|Compound:1486-1332|FD-Score:3.35|P-value:2.02E-4 ID:SGTC_1346|Compound:1488-2224|FD-Score:4.15|P-value:6.20E-6 ID:SGTC_1359|Compound:1582-0056|FD-Score:3.91|P-value:1.86E-5 ID:SGTC_1365|Compound:1672-3266|FD-Score:4.55|P-value:8.53E-7 ID:SGTC_1530|Compound:rhein|FD-Score:3.54|P-value:9.27E-5 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:-3.44|P-value:2.05E-4 ID:SGTC_1635|Compound:st007035|FD-Score:3.69|P-value:4.89E-5 ID:SGTC_1712|Compound:st032288|FD-Score:-3.93|P-value:2.59E-5 ID:SGTC_1727|Compound:st034309|FD-Score:-5.06|P-value:8.56E-8 ID:SGTC_1754|Compound:st042065|FD-Score:-3.54|P-value:1.36E-4 ID:SGTC_1762|Compound:st045503|FD-Score:-3.31|P-value:3.35E-4 ID:SGTC_1786|Compound:berberine|FD-Score:3.99|P-value:1.32E-5 ID:SGTC_1800|Compound:st050079|FD-Score:-4.19|P-value:7.75E-6 ID:SGTC_1806|Compound:st050005|FD-Score:-3.09|P-value:7.47E-4 ID:SGTC_1818|Compound:st052136|FD-Score:-4.28|P-value:5.14E-6 ID:SGTC_1862|Compound:st057513|FD-Score:3.24|P-value:3.10E-4 ID:SGTC_1913|Compound:st060213|FD-Score:3.53|P-value:9.74E-5 ID:SGTC_1972|Compound:st070201|FD-Score:-3.55|P-value:1.28E-4 ID:SGTC_2021|Compound:4019055|FD-Score:-3.28|P-value:3.78E-4 ID:SGTC_2202|Compound:7226349|FD-Score:-3.19|P-value:5.27E-4 ID:SGTC_2264|Compound:7948757|FD-Score:-3.53|P-value:1.39E-4 ID:SGTC_2278|Compound:7948257|FD-Score:3.69|P-value:5.07E-5 ID:SGTC_2338|Compound:7999974|FD-Score:-5.86|P-value:6.99E-10 ID:SGTC_2340|Compound:7987427|FD-Score:3.75|P-value:3.78E-5 ID:SGTC_2341|Compound:7987486|FD-Score:3.21|P-value:3.53E-4 ID:SGTC_2350|Compound:9023895|FD-Score:3.89|P-value:2.07E-5 ID:SGTC_2570|Compound:licareol|FD-Score:3.94|P-value:1.64E-5 ID:SGTC_2766|Compound:oltipraz|FD-Score:2.94|P-value:9.27E-4 ID:SGTC_3012|Compound:9080433|FD-Score:3.41|P-value:1.60E-4 ID:SGTC_3299|Compound:9121982|FD-Score:9.49|P-value:1.45E-23 ID:SGTC_3326|Compound:9140064|FD-Score:-3.15|P-value:6.13E-4 ID:SGTC_3347|Compound:9188721|FD-Score:3.64|P-value:6.11E-5 ID:SGTC_3354|Compound:9151612|FD-Score:3.42|P-value:1.50E-4

Top fitness defect scores for YBR221C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_7 0192-1582 13.00 4.2 µM 1.41E-42 4 4.00E-39
2 SGTC_3299 9121982 9.49 43.1 µM 1.45E-23 1 1.14E-21
3 SGTC_34 0469-0683 6.73 81.8 µM 8.14E-13 SWF1 & branched chain AA biosynthesis 6 8.27E-12
4 SGTC_90 0870-0863 4.74 4.6 µM 3.04E-7 30 1.06E-6
5 SGTC_1365 1672-3266 4.55 55.4 µM 8.53E-7 6 2.71E-6
6 SGTC_73 0327-0325 4.52 16.2 µM 9.70E-7 DNA damage response 32 3.05E-6
7 SGTC_773 4358-1457 4.51 44.6 µM 1.01E-6 29 3.17E-6
8 SGTC_46 0329-0187a 4.26 2.2 µM 3.55E-6 60 1.00E-5
9 SGTC_59 0330-0160 4.20 1.3 µM 4.90E-6 99 1.34E-5
10 SGTC_1346 1488-2224 4.15 62.4 µM 6.20E-6 19 1.67E-5
11 SGTC_1786 berberine 3.99 21.6 µM 1.32E-5 60S ribosome export 30 3.33E-5
12 SGTC_833 3852-0053 3.94 558.0 µM 1.63E-5 17 4.03E-5
13 SGTC_2570 licareol 3.94 100.0 µM 1.64E-5 32 4.07E-5
14 SGTC_1359 1582-0056 3.91 520.0 nM 1.86E-5 20 4.55E-5
15 SGTC_2350 9023895 3.89 38.9 µM 2.07E-5 ergosterol biosynthesis 33 5.04E-5
16 SGTC_2340 7987427 3.75 183.4 µM 3.78E-5 42 8.73E-5
17 SGTC_1635 st007035 3.69 42.4 µM 4.89E-5 227 1.10E-4
18 SGTC_2278 7948257 3.69 134.3 µM 5.07E-5 29 1.14E-4
19 SGTC_3347 9188721 3.64 44.7 µM 6.11E-5 29 1.35E-4
20 SGTC_1132 3456-3614 3.64 2.5 µM 6.21E-5 tubulin folding & SWR complex 68 1.37E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.285 1.69E-63 YER052C HOM3 hom Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
0.251 2.48E-49 YCR053W THR4 hom Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway
0.245 3.72E-47 YNL071W LAT1 hom Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex, which catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA
0.245 5.24E-47 YER061C CEM1 hom Mitochondrial beta-keto-acyl synthase with possible role in fatty acid synthesis; required for mitochondrial respiration
0.236 1.49E-43 YBR068C BAP2 hom High-affinity leucine permease; functions as a branched-chain amino acid permease involved in uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains; BAP2 has a paralog, BAP3, that arose from the whole genome duplication
0.213 1.31E-35 YFL018C LPD1 hom Dihydrolipoamide dehydrogenase, the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase multi-enzyme complexes
0.180 6.53E-26 YPL160W CDC60 het Cytosolic leucyl tRNA synthetase, ligates leucine to the appropriate tRNA
0.178 3.56E-25 YNL215W IES2 hom Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress
0.177 4.60E-25 YHR067W HTD2 hom Mitochondrial 3-hydroxyacyl-thioester dehydratase involved in fatty acid biosynthesis, required for respiratory growth and for normal mitochondrial morphology
0.164 1.11E-21 YHR025W THR1 hom Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway
0.161 9.19E-21 YJR139C HOM6 hom Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions
0.158 3.84E-20 YLR239C LIP2 hom Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups
0.154 3.32E-19 YMR207C HFA1 hom Mitochondrial acetyl-coenzyme A carboxylase; catalyzes the production of malonyl-CoA in mitochondrial fatty acid biosynthesis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress
0.144 5.35E-17 YDL002C NHP10 hom Protein related to mammalian high mobility group proteins; preferentially binds DNA ends, protecting them from exonucleatic cleavage; likely component of the chromatin-remodeling complex INO80 complex; proposed to be involved in DNA repair
0.128 1.20E-13 YLR207W HRD3 hom Resident protein of the ER membrane that plays a central role in ER-associated protein degradation (ERAD), forms HRD complex with Hrd1p and ERAD determinants that engages in lumen to cytosol communication and coordination of ERAD events