YBR223C / TDP1

Tyrosyl-DNA phosphodiesterase I; hydrolyzes 3' and 5'-phosphotyrosyl bonds; involved in the repair of DNA lesions created by topoisomerase I and topoisomerase II; mutations in human homolog result in the neurodegenerative disease SCANI

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR223C / TDP1

Click on Significant Values for Screen Details ID:SGTC_139|Compound:0099-0334|FD-Score:-3.57|P-value:1.70E-4 ID:SGTC_291|Compound:4130-1278|FD-Score:4.20|P-value:1.89E-5 ID:SGTC_340|Compound:k038-0059|FD-Score:3.76|P-value:1.15E-4 ID:SGTC_644|Compound:1498-1197|FD-Score:3.83|P-value:8.72E-5 ID:SGTC_776|Compound:4476-4378|FD-Score:3.59|P-value:2.21E-4 ID:SGTC_866|Compound:0495-0101|FD-Score:3.48|P-value:3.23E-4 ID:SGTC_1046|Compound:2001-0062|FD-Score:4.04|P-value:3.67E-5 ID:SGTC_1051|Compound:3253-3903|FD-Score:6.83|P-value:8.13E-12 ID:SGTC_1061|Compound:3448-9325|FD-Score:4.06|P-value:3.36E-5 ID:SGTC_1090|Compound:efavirenz|FD-Score:5.18|P-value:1.71E-7 ID:SGTC_1091|Compound:telmisartan|FD-Score:3.35|P-value:5.19E-4 ID:SGTC_1105|Compound:epirubicin|FD-Score:4.17|P-value:2.13E-5 ID:SGTC_1177|Compound:0983-0204|FD-Score:-3.09|P-value:9.53E-4 ID:SGTC_1180|Compound:1193-0155|FD-Score:-3.51|P-value:2.15E-4 ID:SGTC_1421|Compound:4029-0249|FD-Score:-3.37|P-value:3.58E-4 ID:SGTC_1469|Compound:k284-2957|FD-Score:3.53|P-value:2.68E-4 ID:SGTC_1591|Compound:genipin|FD-Score:3.85|P-value:8.07E-5 ID:SGTC_1626|Compound:st003712|FD-Score:3.46|P-value:3.46E-4 ID:SGTC_1707|Compound:st032323|FD-Score:6.63|P-value:3.19E-11 ID:SGTC_1936|Compound:st074407|FD-Score:3.24|P-value:7.61E-4 ID:SGTC_2093|Compound:5331342|FD-Score:-3.10|P-value:9.12E-4 ID:SGTC_2125|Compound:5246583|FD-Score:3.82|P-value:8.84E-5 ID:SGTC_2128|Compound:5255449|FD-Score:3.32|P-value:5.80E-4 ID:SGTC_2351|Compound:9026858|FD-Score:-3.09|P-value:9.49E-4 ID:SGTC_2435|Compound:5706476|FD-Score:3.24|P-value:7.54E-4 ID:SGTC_2517|Compound:totarol|FD-Score:3.81|P-value:9.41E-5 ID:SGTC_2765|Compound:myclobutanil|FD-Score:3.71|P-value:1.38E-4 ID:SGTC_2854|Compound:9024559|FD-Score:3.59|P-value:2.14E-4 ID:SGTC_2907|Compound:7721985|FD-Score:-4.04|P-value:2.53E-5 ID:SGTC_3183|Compound:9108214|FD-Score:4.07|P-value:3.28E-5 ID:SGTC_3228|Compound:9131799|FD-Score:3.33|P-value:5.63E-4 ID:SGTC_139|Compound:0099-0334|FD-Score:-3.57|P-value:1.70E-4 ID:SGTC_291|Compound:4130-1278|FD-Score:4.20|P-value:1.89E-5 ID:SGTC_340|Compound:k038-0059|FD-Score:3.76|P-value:1.15E-4 ID:SGTC_644|Compound:1498-1197|FD-Score:3.83|P-value:8.72E-5 ID:SGTC_776|Compound:4476-4378|FD-Score:3.59|P-value:2.21E-4 ID:SGTC_866|Compound:0495-0101|FD-Score:3.48|P-value:3.23E-4 ID:SGTC_1046|Compound:2001-0062|FD-Score:4.04|P-value:3.67E-5 ID:SGTC_1051|Compound:3253-3903|FD-Score:6.83|P-value:8.13E-12 ID:SGTC_1061|Compound:3448-9325|FD-Score:4.06|P-value:3.36E-5 ID:SGTC_1090|Compound:efavirenz|FD-Score:5.18|P-value:1.71E-7 ID:SGTC_1091|Compound:telmisartan|FD-Score:3.35|P-value:5.19E-4 ID:SGTC_1105|Compound:epirubicin|FD-Score:4.17|P-value:2.13E-5 ID:SGTC_1177|Compound:0983-0204|FD-Score:-3.09|P-value:9.53E-4 ID:SGTC_1180|Compound:1193-0155|FD-Score:-3.51|P-value:2.15E-4 ID:SGTC_1421|Compound:4029-0249|FD-Score:-3.37|P-value:3.58E-4 ID:SGTC_1469|Compound:k284-2957|FD-Score:3.53|P-value:2.68E-4 ID:SGTC_1591|Compound:genipin|FD-Score:3.85|P-value:8.07E-5 ID:SGTC_1626|Compound:st003712|FD-Score:3.46|P-value:3.46E-4 ID:SGTC_1707|Compound:st032323|FD-Score:6.63|P-value:3.19E-11 ID:SGTC_1936|Compound:st074407|FD-Score:3.24|P-value:7.61E-4 ID:SGTC_2093|Compound:5331342|FD-Score:-3.10|P-value:9.12E-4 ID:SGTC_2125|Compound:5246583|FD-Score:3.82|P-value:8.84E-5 ID:SGTC_2128|Compound:5255449|FD-Score:3.32|P-value:5.80E-4 ID:SGTC_2351|Compound:9026858|FD-Score:-3.09|P-value:9.49E-4 ID:SGTC_2435|Compound:5706476|FD-Score:3.24|P-value:7.54E-4 ID:SGTC_2517|Compound:totarol|FD-Score:3.81|P-value:9.41E-5 ID:SGTC_2765|Compound:myclobutanil|FD-Score:3.71|P-value:1.38E-4 ID:SGTC_2854|Compound:9024559|FD-Score:3.59|P-value:2.14E-4 ID:SGTC_2907|Compound:7721985|FD-Score:-4.04|P-value:2.53E-5 ID:SGTC_3183|Compound:9108214|FD-Score:4.07|P-value:3.28E-5 ID:SGTC_3228|Compound:9131799|FD-Score:3.33|P-value:5.63E-4

Top fitness defect scores for YBR223C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1051 3253-3903 6.83 78.0 µM 8.13E-12 1 4.14E-12
2 SGTC_1707 st032323 6.63 6.3 µM 3.19E-11 DNA damage response 17 1.67E-11
3 SGTC_1090 efavirenz 5.18 20.7 µM 1.71E-7 7 1.09E-7
4 SGTC_291 4130-1278 4.20 35.5 µM 1.89E-5 Golgi 8 1.36E-5
5 SGTC_1105 epirubicin 4.17 18.1 µM 2.13E-5 anthracycline transcription coupled DNA repair 17 1.53E-5
6 SGTC_3183 9108214 4.07 49.5 µM 3.28E-5 RPP1 & pyrimidine depletion 1 2.38E-5
7 SGTC_1061 3448-9325 4.06 110.0 µM 3.36E-5 22 2.44E-5
8 SGTC_1046 2001-0062 4.04 216.0 µM 3.67E-5 15 2.67E-5
9 SGTC_1591 genipin 3.85 88.4 µM 8.07E-5 mitochondrial processes 16 6.00E-5
10 SGTC_644 1498-1197 3.83 63.3 µM 8.72E-5 26 6.49E-5
11 SGTC_2125 5246583 3.82 200.0 µM 8.84E-5 tubulin folding & SWR complex 48 6.58E-5
12 SGTC_2517 totarol 3.81 10.0 µM 9.41E-5 23 7.02E-5
13 SGTC_340 k038-0059 3.76 454.0 µM 1.15E-4 ubiquinone biosynthesis & proteasome 51 8.60E-5
14 SGTC_2765 myclobutanil 3.71 7.7 µM 1.38E-4 azole & statin 59 1.04E-4
15 SGTC_2854 9024559 3.59 71.4 µM 2.14E-4 35 1.63E-4
16 SGTC_776 4476-4378 3.59 112.0 µM 2.21E-4 ERG2 29 1.68E-4
17 SGTC_1469 k284-2957 3.53 126.0 µM 2.68E-4 18 2.05E-4
18 SGTC_866 0495-0101 3.48 262.0 µM 3.23E-4 60S ribosome export 33 2.49E-4
19 SGTC_1626 st003712 3.46 1.8 µM 3.46E-4 40 2.67E-4
20 SGTC_1091 telmisartan 3.35 47.6 µM 5.19E-4 38 4.05E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.158 3.83E-20 YDL059C RAD59 hom Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p
0.115 2.86E-11 YMR048W CSM3 hom Replication fork associated factor; required for stable replication fork pausing; component of the DNA replication checkpoint pathway; required for accurate chromosome segregation during meiosis; forms nuclear foci upon DNA replication stress
0.111 1.16E-10 YDR386W MUS81 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.105 1.15E-9 YDR075W PPH3 hom Catalytic subunit of protein phosphatase PP4 complex; active complex is composed of Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; involved in activation of Gln3p to alleviate nitrogen catabolite repression; Pph3p and Psy2p localize to foci on meiotic chromosomes
0.103 2.10E-9 YGL175C SAE2 hom Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents
0.097 1.97E-8 YGL163C RAD54 hom DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress
0.092 8.69E-8 YLR307W CDA1 hom Chitin deacetylase, together with Cda2p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall
0.092 1.01E-7 YGR276C RNH70 hom 3'-5' exoribonuclease; required for maturation of 3' ends of 5S rRNA and tRNA-Arg3 from dicistronic transcripts
0.092 1.08E-7 YGL001C ERG26 het C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis
0.091 1.19E-7 YJL092W SRS2 hom DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability
0.087 4.81E-7 YDR076W RAD55 hom Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p
0.084 1.13E-6 YBR095C RXT2 hom Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth
0.082 2.05E-6 YDR004W RAD57 hom Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p
0.081 2.62E-6 YOR156C NFI1 hom SUMO E3 ligase, catalyzes the covalent attachment of SUMO (Smt3p) to proteins; primary E3 ligase for Sir4p; sumoylates Yku70p/Yku80p and Sir4p in vivo to promote chromatin anchoring; promotes telomere anchoring to the nuclear envelope; involved in maintenance of proper telomere length
0.078 5.85E-6 YBR224W_d YBR224W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene TDP1