YBR229C / ROT2

Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR229C / ROT2

Click on Significant Values for Screen Details ID:SGTC_16|Compound:erodoxin|FD-Score:5.56|P-value:1.22E-8 ID:SGTC_22|Compound:0964-0034|FD-Score:9.23|P-value:1.05E-20 ID:SGTC_70|Compound:0141-0289|FD-Score:3.11|P-value:8.91E-4 ID:SGTC_96|Compound:0929-0093|FD-Score:3.97|P-value:3.41E-5 ID:SGTC_98|Compound:0074-0023|FD-Score:3.20|P-value:6.61E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:-4.24|P-value:1.08E-5 ID:SGTC_121|Compound:0557-0495|FD-Score:18.80|P-value:6.57E-80 ID:SGTC_122|Compound:0236-0016|FD-Score:3.12|P-value:8.59E-4 ID:SGTC_124|Compound:0136-0217|FD-Score:4.43|P-value:4.40E-6 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:3.29|P-value:4.80E-4 ID:SGTC_249|Compound:wiskostatin|FD-Score:3.52|P-value:2.03E-4 ID:SGTC_255|Compound:tunicamycin|FD-Score:4.29|P-value:8.32E-6 ID:SGTC_272|Compound:doxorubicin|FD-Score:3.35|P-value:3.90E-4 ID:SGTC_273|Compound:hydroxyurea|FD-Score:3.34|P-value:4.06E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:-3.62|P-value:1.43E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:7.55|P-value:1.85E-14 ID:SGTC_385|Compound:1494-0393|FD-Score:3.28|P-value:4.98E-4 ID:SGTC_390|Compound:dec-rvkr-cmk|FD-Score:7.48|P-value:3.25E-14 ID:SGTC_391|Compound:gliotoxin|FD-Score:5.29|P-value:5.71E-8 ID:SGTC_601|Compound:0986-0029|FD-Score:4.09|P-value:2.06E-5 ID:SGTC_602|Compound:3130-1533|FD-Score:3.83|P-value:6.06E-5 ID:SGTC_655|Compound:k060-0012|FD-Score:-3.15|P-value:8.20E-4 ID:SGTC_686|Compound:1319-0111|FD-Score:-3.09|P-value:9.97E-4 ID:SGTC_708|Compound:3448-5400|FD-Score:3.71|P-value:9.88E-5 ID:SGTC_790|Compound:1575-0012|FD-Score:3.72|P-value:9.65E-5 ID:SGTC_791|Compound:1348-1292|FD-Score:8.26|P-value:5.88E-17 ID:SGTC_800|Compound:3448-7362|FD-Score:6.13|P-value:4.02E-10 ID:SGTC_835|Compound:0312-0010|FD-Score:3.39|P-value:3.29E-4 ID:SGTC_911|Compound:3456-4043|FD-Score:5.68|P-value:6.17E-9 ID:SGTC_921|Compound:1561-0023|FD-Score:-4.03|P-value:2.75E-5 ID:SGTC_930|Compound:2810-3379|FD-Score:-5.66|P-value:7.02E-9 ID:SGTC_934|Compound:2950-0684|FD-Score:8.35|P-value:2.84E-17 ID:SGTC_937|Compound:3122-0005|FD-Score:4.04|P-value:2.51E-5 ID:SGTC_971|Compound:1315-0384|FD-Score:3.21|P-value:6.40E-4 ID:SGTC_978|Compound:1416-0512|FD-Score:4.99|P-value:2.82E-7 ID:SGTC_980|Compound:1473-0395|FD-Score:-3.54|P-value:2.00E-4 ID:SGTC_989|Compound:1501-0895|FD-Score:-3.86|P-value:5.58E-5 ID:SGTC_1163|Compound:k060-0048|FD-Score:3.76|P-value:8.16E-5 ID:SGTC_1224|Compound:0823-0416|FD-Score:-3.45|P-value:2.80E-4 ID:SGTC_1247|Compound:0448-0043|FD-Score:-3.51|P-value:2.24E-4 ID:SGTC_1291|Compound:0993-0025|FD-Score:3.23|P-value:5.99E-4 ID:SGTC_1306|Compound:1185-0270|FD-Score:4.42|P-value:4.63E-6 ID:SGTC_1313|Compound:1220-0047|FD-Score:3.23|P-value:6.00E-4 ID:SGTC_1482|Compound:3346-2049|FD-Score:3.88|P-value:5.05E-5 ID:SGTC_1534|Compound:6-azauridine|FD-Score:3.37|P-value:3.64E-4 ID:SGTC_1541|Compound:2',3',6-trimethoxyflavone|FD-Score:3.75|P-value:8.52E-5 ID:SGTC_1714|Compound:st033236|FD-Score:3.91|P-value:4.36E-5 ID:SGTC_1851|Compound:st056236|FD-Score:3.66|P-value:1.20E-4 ID:SGTC_1860|Compound:st056228|FD-Score:3.71|P-value:9.84E-5 ID:SGTC_1901|Compound:st060216|FD-Score:6.39|P-value:7.24E-11 ID:SGTC_2007|Compound:4011539|FD-Score:9.34|P-value:3.89E-21 ID:SGTC_2008|Compound:4012253|FD-Score:3.79|P-value:7.03E-5 ID:SGTC_2009|Compound:4012297|FD-Score:4.76|P-value:9.21E-7 ID:SGTC_2028|Compound:5153890|FD-Score:3.45|P-value:2.66E-4 ID:SGTC_2042|Compound:p-Dimethylaminoacetophenone|FD-Score:-3.27|P-value:5.29E-4 ID:SGTC_2118|Compound:5526350|FD-Score:3.56|P-value:1.75E-4 ID:SGTC_2130|Compound:NSC 7|FD-Score:3.75|P-value:8.53E-5 ID:SGTC_2144|Compound:5628481|FD-Score:4.00|P-value:3.03E-5 ID:SGTC_2255|Compound:7941087|FD-Score:4.20|P-value:1.24E-5 ID:SGTC_2286|Compound:7945697|FD-Score:4.19|P-value:1.29E-5 ID:SGTC_2289|Compound:7946404|FD-Score:13.00|P-value:3.13E-39 ID:SGTC_2399|Compound:5884133|FD-Score:5.25|P-value:6.86E-8 ID:SGTC_2450|Compound:5739737|FD-Score:3.32|P-value:4.25E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:6.04|P-value:7.05E-10 ID:SGTC_2651|Compound:1327-0036|FD-Score:-4.11|P-value:1.94E-5 ID:SGTC_2737|Compound:bleomycin|FD-Score:7.11|P-value:5.17E-13 ID:SGTC_2920|Compound:7982904|FD-Score:3.43|P-value:2.90E-4 ID:SGTC_3078|Compound:9116413|FD-Score:3.93|P-value:3.99E-5 ID:SGTC_3127|Compound:9126946|FD-Score:3.25|P-value:5.53E-4 ID:SGTC_3304|Compound:9125678|FD-Score:3.31|P-value:4.54E-4 ID:SGTC_16|Compound:erodoxin|FD-Score:5.56|P-value:1.22E-8 ID:SGTC_22|Compound:0964-0034|FD-Score:9.23|P-value:1.05E-20 ID:SGTC_70|Compound:0141-0289|FD-Score:3.11|P-value:8.91E-4 ID:SGTC_96|Compound:0929-0093|FD-Score:3.97|P-value:3.41E-5 ID:SGTC_98|Compound:0074-0023|FD-Score:3.20|P-value:6.61E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:-4.24|P-value:1.08E-5 ID:SGTC_121|Compound:0557-0495|FD-Score:18.80|P-value:6.57E-80 ID:SGTC_122|Compound:0236-0016|FD-Score:3.12|P-value:8.59E-4 ID:SGTC_124|Compound:0136-0217|FD-Score:4.43|P-value:4.40E-6 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:3.29|P-value:4.80E-4 ID:SGTC_249|Compound:wiskostatin|FD-Score:3.52|P-value:2.03E-4 ID:SGTC_255|Compound:tunicamycin|FD-Score:4.29|P-value:8.32E-6 ID:SGTC_272|Compound:doxorubicin|FD-Score:3.35|P-value:3.90E-4 ID:SGTC_273|Compound:hydroxyurea|FD-Score:3.34|P-value:4.06E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:-3.62|P-value:1.43E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:7.55|P-value:1.85E-14 ID:SGTC_385|Compound:1494-0393|FD-Score:3.28|P-value:4.98E-4 ID:SGTC_390|Compound:dec-rvkr-cmk|FD-Score:7.48|P-value:3.25E-14 ID:SGTC_391|Compound:gliotoxin|FD-Score:5.29|P-value:5.71E-8 ID:SGTC_601|Compound:0986-0029|FD-Score:4.09|P-value:2.06E-5 ID:SGTC_602|Compound:3130-1533|FD-Score:3.83|P-value:6.06E-5 ID:SGTC_655|Compound:k060-0012|FD-Score:-3.15|P-value:8.20E-4 ID:SGTC_686|Compound:1319-0111|FD-Score:-3.09|P-value:9.97E-4 ID:SGTC_708|Compound:3448-5400|FD-Score:3.71|P-value:9.88E-5 ID:SGTC_790|Compound:1575-0012|FD-Score:3.72|P-value:9.65E-5 ID:SGTC_791|Compound:1348-1292|FD-Score:8.26|P-value:5.88E-17 ID:SGTC_800|Compound:3448-7362|FD-Score:6.13|P-value:4.02E-10 ID:SGTC_835|Compound:0312-0010|FD-Score:3.39|P-value:3.29E-4 ID:SGTC_911|Compound:3456-4043|FD-Score:5.68|P-value:6.17E-9 ID:SGTC_921|Compound:1561-0023|FD-Score:-4.03|P-value:2.75E-5 ID:SGTC_930|Compound:2810-3379|FD-Score:-5.66|P-value:7.02E-9 ID:SGTC_934|Compound:2950-0684|FD-Score:8.35|P-value:2.84E-17 ID:SGTC_937|Compound:3122-0005|FD-Score:4.04|P-value:2.51E-5 ID:SGTC_971|Compound:1315-0384|FD-Score:3.21|P-value:6.40E-4 ID:SGTC_978|Compound:1416-0512|FD-Score:4.99|P-value:2.82E-7 ID:SGTC_980|Compound:1473-0395|FD-Score:-3.54|P-value:2.00E-4 ID:SGTC_989|Compound:1501-0895|FD-Score:-3.86|P-value:5.58E-5 ID:SGTC_1163|Compound:k060-0048|FD-Score:3.76|P-value:8.16E-5 ID:SGTC_1224|Compound:0823-0416|FD-Score:-3.45|P-value:2.80E-4 ID:SGTC_1247|Compound:0448-0043|FD-Score:-3.51|P-value:2.24E-4 ID:SGTC_1291|Compound:0993-0025|FD-Score:3.23|P-value:5.99E-4 ID:SGTC_1306|Compound:1185-0270|FD-Score:4.42|P-value:4.63E-6 ID:SGTC_1313|Compound:1220-0047|FD-Score:3.23|P-value:6.00E-4 ID:SGTC_1482|Compound:3346-2049|FD-Score:3.88|P-value:5.05E-5 ID:SGTC_1534|Compound:6-azauridine|FD-Score:3.37|P-value:3.64E-4 ID:SGTC_1541|Compound:2',3',6-trimethoxyflavone|FD-Score:3.75|P-value:8.52E-5 ID:SGTC_1714|Compound:st033236|FD-Score:3.91|P-value:4.36E-5 ID:SGTC_1851|Compound:st056236|FD-Score:3.66|P-value:1.20E-4 ID:SGTC_1860|Compound:st056228|FD-Score:3.71|P-value:9.84E-5 ID:SGTC_1901|Compound:st060216|FD-Score:6.39|P-value:7.24E-11 ID:SGTC_2007|Compound:4011539|FD-Score:9.34|P-value:3.89E-21 ID:SGTC_2008|Compound:4012253|FD-Score:3.79|P-value:7.03E-5 ID:SGTC_2009|Compound:4012297|FD-Score:4.76|P-value:9.21E-7 ID:SGTC_2028|Compound:5153890|FD-Score:3.45|P-value:2.66E-4 ID:SGTC_2042|Compound:p-Dimethylaminoacetophenone|FD-Score:-3.27|P-value:5.29E-4 ID:SGTC_2118|Compound:5526350|FD-Score:3.56|P-value:1.75E-4 ID:SGTC_2130|Compound:NSC 7|FD-Score:3.75|P-value:8.53E-5 ID:SGTC_2144|Compound:5628481|FD-Score:4.00|P-value:3.03E-5 ID:SGTC_2255|Compound:7941087|FD-Score:4.20|P-value:1.24E-5 ID:SGTC_2286|Compound:7945697|FD-Score:4.19|P-value:1.29E-5 ID:SGTC_2289|Compound:7946404|FD-Score:13.00|P-value:3.13E-39 ID:SGTC_2399|Compound:5884133|FD-Score:5.25|P-value:6.86E-8 ID:SGTC_2450|Compound:5739737|FD-Score:3.32|P-value:4.25E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:6.04|P-value:7.05E-10 ID:SGTC_2651|Compound:1327-0036|FD-Score:-4.11|P-value:1.94E-5 ID:SGTC_2737|Compound:bleomycin|FD-Score:7.11|P-value:5.17E-13 ID:SGTC_2920|Compound:7982904|FD-Score:3.43|P-value:2.90E-4 ID:SGTC_3078|Compound:9116413|FD-Score:3.93|P-value:3.99E-5 ID:SGTC_3127|Compound:9126946|FD-Score:3.25|P-value:5.53E-4 ID:SGTC_3304|Compound:9125678|FD-Score:3.31|P-value:4.54E-4

Top fitness defect scores for YBR229C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_121 0557-0495 18.80 61.3 µM 6.57E-80 calcium & mitochondrial duress 1 1.57E-79
2 SGTC_2289 7946404 13.00 165.1 µM 3.13E-39 3 4.84E-39
3 SGTC_2007 4011539 9.34 71.8 µM 3.89E-21 cell wall signaling 13 4.92E-21
4 SGTC_22 0964-0034 9.23 14.5 µM 1.05E-20 unfolded protein response 8 1.32E-20
5 SGTC_934 2950-0684 8.35 338.0 µM 2.84E-17 2 3.45E-17
6 SGTC_791 1348-1292 8.26 225.0 µM 5.88E-17 3 7.12E-17
7 SGTC_351 caspofungin 7.55 25.0 nM 1.85E-14 cell wall 19 2.17E-14
8 SGTC_390 dec-rvkr-cmk 7.48 16.7 µM 3.25E-14 calcium & mitochondrial duress 4 3.81E-14
9 SGTC_2737 bleomycin 7.11 30.5 nM 5.17E-13 Golgi 22 5.98E-13
10 SGTC_1901 st060216 6.39 58.6 µM 7.24E-11 3 8.17E-11
11 SGTC_800 3448-7362 6.13 155.0 µM 4.02E-10 translation 45 4.49E-10
12 SGTC_2619 obliquin 6.04 87.9 µM 7.05E-10 cell wall 74 7.87E-10
13 SGTC_911 3456-4043 5.68 236.0 µM 6.17E-9 mitochondrial processes 5 6.82E-9
14 SGTC_16 erodoxin 5.56 22.8 µM 1.22E-8 unfolded protein response 30 1.35E-8
15 SGTC_391 gliotoxin 5.29 8.3 µM 5.71E-8 34 6.23E-8
16 SGTC_2399 5884133 5.25 200.0 µM 6.86E-8 cell wall signaling 37 7.48E-8
17 SGTC_978 1416-0512 4.99 87.4 µM 2.82E-7 redox potentiating 14 3.05E-7
18 SGTC_2009 4012297 4.76 12.1 µM 9.21E-7 cell wall signaling 40 9.92E-7
19 SGTC_124 0136-0217 4.43 7.1 µM 4.40E-6 mitochondrial stress 47 4.70E-6
20 SGTC_1306 1185-0270 4.42 9.0 µM 4.63E-6 Golgi 44 4.94E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.335 7.67E-89 YNL322C KRE1 hom Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor
0.299 3.18E-70 YGL027C CWH41 hom Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress
0.266 1.96E-55 YOR085W OST3 hom Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins
0.244 1.08E-46 YBR260C RGD1 hom GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization
0.242 8.72E-46 YAL056C-A_d YAL056C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.236 1.12E-43 YAL058W CNE1 hom Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast
0.228 1.17E-40 YLR120C YPS1 hom Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor
0.225 7.46E-40 YDL117W CYK3 hom SH3-domain protein located in the bud neck and cytokinetic actin ring; relocalizes from bud neck to nucleus upon DNA replication stress; activates the chitin synthase activity of Chs2p during cytokinesis; suppressor of growth and cytokinesis defects of chs2 phospho-mutants
0.220 3.43E-38 YGR196C FYV8 hom Protein of unknown function, required for survival upon exposure to K1 killer toxin
0.217 4.66E-37 YKL096C-B_p YKL096C-B_p hom Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
0.212 2.63E-35 YMR200W ROT1 het Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation
0.186 1.96E-27 YDL096C_d OPI6_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential
0.179 1.58E-25 YEL036C ANP1 hom Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol
0.177 4.55E-25 YOR216C RUD3 hom Golgi matrix protein involved in the structural organization of the cis-Golgi; interacts genetically with COG3 and USO1
0.177 6.00E-25 YOR109W INP53 hom Polyphosphatidylinositol phosphatase, dephosphorylates multiple phosphatidylinositols; involved in trans Golgi network-to-early endosome pathway; hyperosmotic stress causes translocation to actin patches; contains Sac1 and 5-ptase domains