YBR235W / VHC1

Vacuolar membrane cation-chloride cotransporter (CCC); likely mediates K+ and Cl- cotransport into the vacuole; has a role in potassium homeostasis and salt tolerance; similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR235W / VHC1

Click on Significant Values for Screen Details

Top fitness defect scores for YBR235W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
1 SGTC_459 nigericin 12.80 15.3 µM 2.54E-35 2 9.94E-38
2 SGTC_2366 9070441 6.38 60.6 µM 3.86E-10 3 9.07E-11
3 SGTC_1492 4158-0558 4.98 28.5 µM 7.75E-7 60S ribosome export 5 3.11E-7
4 SGTC_1871 st057448 4.57 67.3 µM 5.43E-6 11 2.50E-6
5 SGTC_1255 0566-0045 4.40 221.0 µM 1.13E-5 excess fatty acid 23 5.49E-6
6 SGTC_230 MPP+ 4.37 141.7 µM 1.27E-5 11 6.19E-6
7 SGTC_2268 7933382 4.37 153.8 µM 1.27E-5 27 6.19E-6
8 SGTC_1694 st024011 4.34 44.9 µM 1.45E-5 ERG2 30 7.16E-6
9 SGTC_1294 1000-1494 4.05 117.0 µM 4.87E-5 21 2.61E-5
10 SGTC_1169 aniline yellow 3.98 25.8 µM 6.31E-5 8 3.44E-5
11 SGTC_769 0302-0231 3.89 76.0 µM 9.02E-5 42 5.04E-5
12 SGTC_2186 6124449 3.39 200.0 µM 5.51E-4 RSC complex & mRNA processing 36 3.50E-4
13 SGTC_2263 7948595 3.38 193.3 µM 5.73E-4 18 3.65E-4
14 SGTC_1584 6-fluoro-dl-tryptophan 3.37 90.0 µM 5.98E-4 mitochondrial processes 35 3.81E-4
15 SGTC_1496 4469-0713 3.33 57.4 µM 6.73E-4 37 4.33E-4
16 SGTC_1511 0906-3109 3.32 28.5 µM 7.02E-4 excess fatty acid 80 4.53E-4
17 SGTC_2605 digitonin 3.25 300.0 nM 8.76E-4 62 5.74E-4
18 SGTC_2086 5274770 3.22 176.6 µM 9.61E-4 56 6.33E-4
19 SGTC_1073 triclabendazole 3.20 3.1 µM 0.00105 72 6.95E-4
20 SGTC_801 0866-0028 3.15 10.1 µM 0.00120 77 8.03E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.140 4.63E-16 YDL207W GLE1 het Cytoplasmic nucleoporin required for polyadenylated RNA export; not essential for protein import; contains a nuclear export signal; when bound to inositol hexakisphosphate (IP6), functions as an activator for the Dbp5p ATPase activity at the nuclear pore complex during mRNA export
0.133 8.32E-15 YOL122C SMF1 hom Divalent metal ion transporter with a broad specificity for di-valent and tri-valent metals; post-translationally regulated by levels of metal ions; member of the Nramp family of metal transport proteins
0.117 1.28E-11 YNL155W YNL155W hom Protein of unknown function; contains a DHHC domain and an AN1-type zinc finger; predicted to have thiol-disulfide oxidoreductase active site; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress
0.102 3.58E-9 YLL031C GPI13 het ER membrane localized phosphoryltransferase that adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein
0.101 4.62E-9 YGL130W CEG1 het Alpha (guanylyltransferase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CET1, an RNA 5'-triphosphatase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide
0.099 1.02E-8 YPL123C RNY1 hom Vacuolar RNase of the T(2) family, relocalizes to the cytosol where it cleaves tRNAs upon oxidative or stationary phase stress; promotes apoptosis under stress conditions and this function is independent of its catalytic activity
0.097 1.80E-8 YJL181W_p YJL181W_p hom Putative protein of unknown function; expression is cell-cycle regulated as shown by microarray analysis; potential regulatory target of Mbp1p, which binds to the YJL181W promoter region; YJL181W has a paralog, YJR030C, that arose from the whole genome duplication
0.092 8.86E-8 YDR479C PEX29 hom Peroxisomal integral membrane peroxin; involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress
0.092 9.77E-8 YBR258C SHG1 hom Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres
0.091 1.20E-7 YDR080W VPS41 hom Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport
0.091 1.36E-7 YPR033C HTS1 het Cytoplasmic and mitochondrial histidine tRNA synthetase; efficient mitochondrial localization requires both a presequence and an amino-terminal sequence; mutations in human ortholog HARS2 are associated with Perrault syndrome
0.089 2.39E-7 YLR296W_d YLR296W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.089 2.48E-7 YLL025W PAU17 hom Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions; YLL025W is not an essential gene
0.088 3.11E-7 YGL138C_p YGL138C_p hom Putative protein of unknown function; has no significant sequence similarity to any known protein
0.086 5.40E-7 YLR444C_d YLR444C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data