YBR245C / ISW1

ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; ATPase; forms a complex with Ioc3p (Isw1a), and a complex with Ioc2p and Ioc4p (Isw1b); Isw1a and Isw1b have partially overlapping and distinct roles, Isw1a involved in repression of transcription initiation and Isw1b involved in regulation of transcription elongation; Isw1b recruited to open reading frames by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR245C / ISW1

Click on Significant Values for Screen Details ID:SGTC_50|Compound:0929-0078|FD-Score:-4.28|P-value:5.58E-6 ID:SGTC_52|Compound:0329-0193|FD-Score:-3.62|P-value:1.02E-4 ID:SGTC_54|Compound:0159-0055|FD-Score:5.34|P-value:1.75E-8 ID:SGTC_90|Compound:0870-0863|FD-Score:3.81|P-value:3.99E-5 ID:SGTC_172|Compound:1112-0046|FD-Score:4.43|P-value:2.31E-6 ID:SGTC_178|Compound:4092-0806|FD-Score:4.34|P-value:3.63E-6 ID:SGTC_221|Compound:trifluoperazine|FD-Score:15.90|P-value:1.55E-60 ID:SGTC_254|Compound:nsc-64875|FD-Score:2.99|P-value:9.81E-4 ID:SGTC_378|Compound:3970-0959|FD-Score:4.24|P-value:5.93E-6 ID:SGTC_439|Compound:amiodarone|FD-Score:3.76|P-value:4.94E-5 ID:SGTC_445|Compound:e6 berbamine|FD-Score:3.80|P-value:4.27E-5 ID:SGTC_528|Compound:3492-0019|FD-Score:4.20|P-value:6.89E-6 ID:SGTC_568|Compound:1193-0097|FD-Score:4.00|P-value:1.79E-5 ID:SGTC_606|Compound:k015-0008|FD-Score:4.04|P-value:1.46E-5 ID:SGTC_770|Compound:0389-1121|FD-Score:4.30|P-value:4.32E-6 ID:SGTC_822|Compound:1487-0950|FD-Score:3.02|P-value:8.84E-4 ID:SGTC_955|Compound:1216-0110|FD-Score:3.93|P-value:2.40E-5 ID:SGTC_1414|Compound:3917-0015|FD-Score:3.32|P-value:2.95E-4 ID:SGTC_1521|Compound:st012942|FD-Score:3.39|P-value:2.22E-4 ID:SGTC_1577|Compound:3(2'-chlorophenyl)-7-ethoxycoumarin|FD-Score:4.96|P-value:1.48E-7 ID:SGTC_1712|Compound:st032288|FD-Score:6.56|P-value:6.26E-12 ID:SGTC_1745|Compound:st081495|FD-Score:3.01|P-value:9.06E-4 ID:SGTC_1754|Compound:st042065|FD-Score:9.39|P-value:1.59E-22 ID:SGTC_1800|Compound:st050079|FD-Score:4.67|P-value:6.83E-7 ID:SGTC_1801|Compound:st050089|FD-Score:3.58|P-value:1.07E-4 ID:SGTC_1803|Compound:st050068|FD-Score:4.96|P-value:1.50E-7 ID:SGTC_1818|Compound:st052136|FD-Score:5.02|P-value:1.06E-7 ID:SGTC_1849|Compound:st056239|FD-Score:-3.35|P-value:2.93E-4 ID:SGTC_1912|Compound:st060209|FD-Score:7.56|P-value:3.00E-15 ID:SGTC_1923|Compound:st058458|FD-Score:3.51|P-value:1.42E-4 ID:SGTC_2021|Compound:4019055|FD-Score:4.41|P-value:2.56E-6 ID:SGTC_2022|Compound:5136654|FD-Score:4.11|P-value:1.06E-5 ID:SGTC_2084|Compound:5306078|FD-Score:3.07|P-value:7.25E-4 ID:SGTC_2127|Compound:5253358|FD-Score:3.90|P-value:2.75E-5 ID:SGTC_2128|Compound:5255449|FD-Score:3.49|P-value:1.53E-4 ID:SGTC_2129|Compound:girgensonine|FD-Score:-3.94|P-value:2.59E-5 ID:SGTC_2398|Compound:5228213|FD-Score:3.50|P-value:1.44E-4 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:3.76|P-value:4.93E-5 ID:SGTC_2418|Compound:pentamidine|FD-Score:3.09|P-value:6.94E-4 ID:SGTC_2445|Compound:5742597|FD-Score:5.08|P-value:7.53E-8 ID:SGTC_2448|Compound:5185112|FD-Score:-3.28|P-value:3.92E-4 ID:SGTC_2449|Compound:5812982|FD-Score:-3.14|P-value:6.45E-4 ID:SGTC_2477|Compound:5967686|FD-Score:4.61|P-value:9.25E-7 ID:SGTC_2485|Compound:5633444|FD-Score:4.04|P-value:1.45E-5 ID:SGTC_2503|Compound:beta-caryophyllene alcohol|FD-Score:-4.72|P-value:6.31E-7 ID:SGTC_2570|Compound:licareol|FD-Score:5.43|P-value:1.04E-8 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:5.92|P-value:4.70E-10 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:-4.25|P-value:6.50E-6 ID:SGTC_2575|Compound:plumbagin|FD-Score:-4.32|P-value:4.62E-6 ID:SGTC_2611|Compound:pyromycin|FD-Score:3.10|P-value:6.73E-4 ID:SGTC_2725|Compound:haloperidol|FD-Score:3.67|P-value:7.17E-5 ID:SGTC_2752|Compound:diclazuril|FD-Score:4.15|P-value:8.71E-6 ID:SGTC_2864|Compound:9034755|FD-Score:3.70|P-value:6.40E-5 ID:SGTC_2896|Compound:9052656|FD-Score:5.17|P-value:4.52E-8 ID:SGTC_2973|Compound:9094665|FD-Score:3.13|P-value:5.99E-4 ID:SGTC_2993|Compound:9054257|FD-Score:4.49|P-value:1.68E-6 ID:SGTC_3036|Compound:9090181|FD-Score:3.26|P-value:3.68E-4 ID:SGTC_3037|Compound:9091026|FD-Score:8.21|P-value:1.21E-17 ID:SGTC_3200|Compound:9114385|FD-Score:3.47|P-value:1.66E-4 ID:SGTC_3296|Compound:9122258|FD-Score:3.10|P-value:6.59E-4 ID:SGTC_50|Compound:0929-0078|FD-Score:-4.28|P-value:5.58E-6 ID:SGTC_52|Compound:0329-0193|FD-Score:-3.62|P-value:1.02E-4 ID:SGTC_54|Compound:0159-0055|FD-Score:5.34|P-value:1.75E-8 ID:SGTC_90|Compound:0870-0863|FD-Score:3.81|P-value:3.99E-5 ID:SGTC_172|Compound:1112-0046|FD-Score:4.43|P-value:2.31E-6 ID:SGTC_178|Compound:4092-0806|FD-Score:4.34|P-value:3.63E-6 ID:SGTC_221|Compound:trifluoperazine|FD-Score:15.90|P-value:1.55E-60 ID:SGTC_254|Compound:nsc-64875|FD-Score:2.99|P-value:9.81E-4 ID:SGTC_378|Compound:3970-0959|FD-Score:4.24|P-value:5.93E-6 ID:SGTC_439|Compound:amiodarone|FD-Score:3.76|P-value:4.94E-5 ID:SGTC_445|Compound:e6 berbamine|FD-Score:3.80|P-value:4.27E-5 ID:SGTC_528|Compound:3492-0019|FD-Score:4.20|P-value:6.89E-6 ID:SGTC_568|Compound:1193-0097|FD-Score:4.00|P-value:1.79E-5 ID:SGTC_606|Compound:k015-0008|FD-Score:4.04|P-value:1.46E-5 ID:SGTC_770|Compound:0389-1121|FD-Score:4.30|P-value:4.32E-6 ID:SGTC_822|Compound:1487-0950|FD-Score:3.02|P-value:8.84E-4 ID:SGTC_955|Compound:1216-0110|FD-Score:3.93|P-value:2.40E-5 ID:SGTC_1414|Compound:3917-0015|FD-Score:3.32|P-value:2.95E-4 ID:SGTC_1521|Compound:st012942|FD-Score:3.39|P-value:2.22E-4 ID:SGTC_1577|Compound:3(2'-chlorophenyl)-7-ethoxycoumarin|FD-Score:4.96|P-value:1.48E-7 ID:SGTC_1712|Compound:st032288|FD-Score:6.56|P-value:6.26E-12 ID:SGTC_1745|Compound:st081495|FD-Score:3.01|P-value:9.06E-4 ID:SGTC_1754|Compound:st042065|FD-Score:9.39|P-value:1.59E-22 ID:SGTC_1800|Compound:st050079|FD-Score:4.67|P-value:6.83E-7 ID:SGTC_1801|Compound:st050089|FD-Score:3.58|P-value:1.07E-4 ID:SGTC_1803|Compound:st050068|FD-Score:4.96|P-value:1.50E-7 ID:SGTC_1818|Compound:st052136|FD-Score:5.02|P-value:1.06E-7 ID:SGTC_1849|Compound:st056239|FD-Score:-3.35|P-value:2.93E-4 ID:SGTC_1912|Compound:st060209|FD-Score:7.56|P-value:3.00E-15 ID:SGTC_1923|Compound:st058458|FD-Score:3.51|P-value:1.42E-4 ID:SGTC_2021|Compound:4019055|FD-Score:4.41|P-value:2.56E-6 ID:SGTC_2022|Compound:5136654|FD-Score:4.11|P-value:1.06E-5 ID:SGTC_2084|Compound:5306078|FD-Score:3.07|P-value:7.25E-4 ID:SGTC_2127|Compound:5253358|FD-Score:3.90|P-value:2.75E-5 ID:SGTC_2128|Compound:5255449|FD-Score:3.49|P-value:1.53E-4 ID:SGTC_2129|Compound:girgensonine|FD-Score:-3.94|P-value:2.59E-5 ID:SGTC_2398|Compound:5228213|FD-Score:3.50|P-value:1.44E-4 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:3.76|P-value:4.93E-5 ID:SGTC_2418|Compound:pentamidine|FD-Score:3.09|P-value:6.94E-4 ID:SGTC_2445|Compound:5742597|FD-Score:5.08|P-value:7.53E-8 ID:SGTC_2448|Compound:5185112|FD-Score:-3.28|P-value:3.92E-4 ID:SGTC_2449|Compound:5812982|FD-Score:-3.14|P-value:6.45E-4 ID:SGTC_2477|Compound:5967686|FD-Score:4.61|P-value:9.25E-7 ID:SGTC_2485|Compound:5633444|FD-Score:4.04|P-value:1.45E-5 ID:SGTC_2503|Compound:beta-caryophyllene alcohol|FD-Score:-4.72|P-value:6.31E-7 ID:SGTC_2570|Compound:licareol|FD-Score:5.43|P-value:1.04E-8 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:5.92|P-value:4.70E-10 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:-4.25|P-value:6.50E-6 ID:SGTC_2575|Compound:plumbagin|FD-Score:-4.32|P-value:4.62E-6 ID:SGTC_2611|Compound:pyromycin|FD-Score:3.10|P-value:6.73E-4 ID:SGTC_2725|Compound:haloperidol|FD-Score:3.67|P-value:7.17E-5 ID:SGTC_2752|Compound:diclazuril|FD-Score:4.15|P-value:8.71E-6 ID:SGTC_2864|Compound:9034755|FD-Score:3.70|P-value:6.40E-5 ID:SGTC_2896|Compound:9052656|FD-Score:5.17|P-value:4.52E-8 ID:SGTC_2973|Compound:9094665|FD-Score:3.13|P-value:5.99E-4 ID:SGTC_2993|Compound:9054257|FD-Score:4.49|P-value:1.68E-6 ID:SGTC_3036|Compound:9090181|FD-Score:3.26|P-value:3.68E-4 ID:SGTC_3037|Compound:9091026|FD-Score:8.21|P-value:1.21E-17 ID:SGTC_3200|Compound:9114385|FD-Score:3.47|P-value:1.66E-4 ID:SGTC_3296|Compound:9122258|FD-Score:3.10|P-value:6.59E-4

Top fitness defect scores for YBR245C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_221 trifluoperazine 15.90 10.3 µM 1.55E-60 NEO1-PIK1 22 4.83E-57
2 SGTC_1754 st042065 9.39 16.1 µM 1.59E-22 NEO1-PIK1 34 2.89E-21
3 SGTC_3037 9091026 8.21 49.5 µM 1.21E-17 NEO1-PIK1 13 1.14E-16
4 SGTC_1912 st060209 7.56 90.8 µM 3.00E-15 2 2.03E-14
5 SGTC_1712 st032288 6.56 53.4 µM 6.26E-12 calcium & mitochondrial duress 65 2.71E-11
6 SGTC_2572 astragaloside iv 5.92 100.0 µM 4.70E-10 3 1.58E-9
7 SGTC_2570 licareol 5.43 100.0 µM 1.04E-8 8 2.90E-8
8 SGTC_54 0159-0055 5.34 9.0 µM 1.75E-8 ERG2 25 4.74E-8
9 SGTC_2896 9052656 5.17 71.4 µM 4.52E-8 endomembrane recycling 54 1.16E-7
10 SGTC_2445 5742597 5.08 35.6 µM 7.53E-8 19 1.87E-7
11 SGTC_1818 st052136 5.02 56.3 µM 1.06E-7 NEO1-PIK1 24 2.57E-7
12 SGTC_1577 3(2'-chlorophenyl)-7-ethoxycoumarin 4.96 66.5 µM 1.48E-7 7 3.54E-7
13 SGTC_1803 st050068 4.96 52.4 µM 1.50E-7 NEO1-PIK1 116 3.58E-7
14 SGTC_1800 st050079 4.67 29.1 µM 6.83E-7 NEO1-PIK1 88 1.49E-6
15 SGTC_2477 5967686 4.61 25.2 µM 9.25E-7 17 1.98E-6
16 SGTC_2993 9054257 4.49 71.4 µM 1.68E-6 fatty acid desaturase (OLE1) 28 3.49E-6
17 SGTC_172 1112-0046 4.43 2.6 µM 2.31E-6 NEO1 28 4.69E-6
18 SGTC_2021 4019055 4.41 173.0 µM 2.56E-6 endomembrane recycling 96 5.17E-6
19 SGTC_178 4092-0806 4.34 87.5 µM 3.63E-6 ergosterol depletion effects on membrane 75 7.19E-6
20 SGTC_770 0389-1121 4.30 67.2 µM 4.32E-6 RPP1 & pyrimidine depletion 17 8.47E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.432 1.01E-152 YBR246W RRT2 hom Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription
0.387 1.69E-120 YML037C_p YML037C_p hom Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene
0.377 1.47E-113 YNL183C NPR1 hom Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex
0.376 4.29E-113 YOR357C SNX3 hom Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p
0.361 1.41E-103 YOR115C TRS33 hom One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic
0.359 3.40E-102 YNL267W PIK1 het Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton
0.355 2.65E-100 YPR091C NVJ2 hom Lipid-binding ER protein, enriched at nucleus-vacuolar junctions (NVJ); may be involved in sterol metabolism or signaling at the NVJ; contains a synaptotagmin-like-mitochondrial- lipid binding protein (SMP) domain; binds phosphatidylinositols and other lipids in a large-scale study; may interact with ribosomes, based on co-purification experiments
0.349 1.06E-96 YGL077C HNM1 hom Choline/ethanolamine transporter; involved in the uptake of nitrogen mustard and the uptake of glycine betaine during hypersaline stress; co-regulated with phospholipid biosynthetic genes and negatively regulated by choline and myo-inositol
0.339 6.78E-91 YJL052W TDH1 hom Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress
0.325 3.00E-83 YGR166W TRS65 hom Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response
0.324 8.91E-83 YJL154C VPS35 hom Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p
0.322 5.91E-82 YGL035C MIG1 hom Transcription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase; regulates filamentous growth along with Mig2p in response to glucose depletion
0.319 3.12E-80 YLR020C YEH2 hom Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism
0.305 1.92E-73 YER120W SCS2 hom Integral ER membrane protein, regulates phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PI4P levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; interacts with FFAT motif of Opi1p; involved in telomeric silencing; null shows inositol auxotrophy above 34 deg C; VAP homolog; SCS2 has a paralog, SCS22, that arose from the whole genome duplication
0.305 3.08E-73 YKR095W MLP1 hom Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved with Tel1p in telomere length control; involved with Pml1p and Pml39p in nuclear retention of unspliced mRNAs