YBR253W / SRB6

Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation

Zygosity: Heterozygous strain
fixedexpanded
Profile for YBR253W / SRB6

Click on Significant Values for Screen Details ID:SGTC_11|Compound:0438-0307|FD-Score:3.46|P-value:3.59E-4 ID:SGTC_81|Compound:0411-0046|FD-Score:3.75|P-value:1.23E-4 ID:SGTC_264|Compound:promethazine|FD-Score:4.11|P-value:2.97E-5 ID:SGTC_489|Compound:paxilline|FD-Score:3.22|P-value:8.09E-4 ID:SGTC_512|Compound:prima-1|FD-Score:4.51|P-value:5.22E-6 ID:SGTC_515|Compound:4092-1064|FD-Score:-3.22|P-value:8.80E-4 ID:SGTC_516|Compound:3992-2719|FD-Score:-3.54|P-value:2.94E-4 ID:SGTC_691|Compound:1548-0686|FD-Score:-3.74|P-value:1.41E-4 ID:SGTC_700|Compound:3453-2283|FD-Score:3.42|P-value:4.19E-4 ID:SGTC_972|Compound:1319-0219|FD-Score:-3.19|P-value:9.72E-4 ID:SGTC_978|Compound:1416-0512|FD-Score:4.89|P-value:8.91E-7 ID:SGTC_980|Compound:1473-0395|FD-Score:3.58|P-value:2.33E-4 ID:SGTC_987|Compound:1497-1013|FD-Score:-3.83|P-value:1.01E-4 ID:SGTC_1067|Compound:clotrimazole|FD-Score:3.41|P-value:4.35E-4 ID:SGTC_1068|Compound:loratadine|FD-Score:4.48|P-value:5.95E-6 ID:SGTC_1071|Compound:trifluoperazine|FD-Score:-3.30|P-value:6.81E-4 ID:SGTC_1076|Compound:itraconazole|FD-Score:3.96|P-value:5.53E-5 ID:SGTC_1203|Compound:3992-3522|FD-Score:5.54|P-value:3.16E-8 ID:SGTC_1206|Compound:3978-0017|FD-Score:4.32|P-value:1.23E-5 ID:SGTC_1252|Compound:0493-0875|FD-Score:6.41|P-value:1.94E-10 ID:SGTC_1281|Compound:0884-0031|FD-Score:3.55|P-value:2.64E-4 ID:SGTC_1322|Compound:1348-1070|FD-Score:3.35|P-value:5.31E-4 ID:SGTC_1350|Compound:1495-0454|FD-Score:-3.35|P-value:5.68E-4 ID:SGTC_1419|Compound:3993-2473|FD-Score:4.06|P-value:3.71E-5 ID:SGTC_1513|Compound:0337-0559|FD-Score:-3.23|P-value:8.66E-4 ID:SGTC_1515|Compound:3460-0031|FD-Score:3.26|P-value:7.08E-4 ID:SGTC_1525|Compound:8-methoxypsoralen|FD-Score:3.35|P-value:5.30E-4 ID:SGTC_1577|Compound:3(2'-chlorophenyl)-7-ethoxycoumarin|FD-Score:-3.27|P-value:7.44E-4 ID:SGTC_1586|Compound:deoxycorticosterone acetate|FD-Score:-3.61|P-value:2.25E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:3.57|P-value:2.45E-4 ID:SGTC_2022|Compound:5136654|FD-Score:-3.20|P-value:9.30E-4 ID:SGTC_2135|Compound:5326629|FD-Score:3.91|P-value:6.67E-5 ID:SGTC_2173|Compound:5763948|FD-Score:3.34|P-value:5.57E-4 ID:SGTC_2541|Compound:muurolladie-3-one|FD-Score:3.32|P-value:5.77E-4 ID:SGTC_2544|Compound:sphondin|FD-Score:4.16|P-value:2.38E-5 ID:SGTC_2780|Compound:5215298|FD-Score:4.97|P-value:5.99E-7 ID:SGTC_2832|Compound:7992762|FD-Score:-4.19|P-value:2.37E-5 ID:SGTC_2937|Compound:9044753|FD-Score:3.26|P-value:7.09E-4 ID:SGTC_2948|Compound:9070189|FD-Score:5.54|P-value:3.19E-8 ID:SGTC_2975|Compound:9084847|FD-Score:-3.47|P-value:3.74E-4 ID:SGTC_3142|Compound:9086498|FD-Score:4.34|P-value:1.15E-5 ID:SGTC_3154|Compound:9100113|FD-Score:4.77|P-value:1.59E-6 ID:SGTC_3229|Compound:9132204|FD-Score:3.58|P-value:2.38E-4 ID:SGTC_3234|Compound:9132280|FD-Score:4.80|P-value:1.41E-6 ID:SGTC_3348|Compound:9149464|FD-Score:-4.95|P-value:7.37E-7 ID:SGTC_11|Compound:0438-0307|FD-Score:3.46|P-value:3.59E-4 ID:SGTC_81|Compound:0411-0046|FD-Score:3.75|P-value:1.23E-4 ID:SGTC_264|Compound:promethazine|FD-Score:4.11|P-value:2.97E-5 ID:SGTC_489|Compound:paxilline|FD-Score:3.22|P-value:8.09E-4 ID:SGTC_512|Compound:prima-1|FD-Score:4.51|P-value:5.22E-6 ID:SGTC_515|Compound:4092-1064|FD-Score:-3.22|P-value:8.80E-4 ID:SGTC_516|Compound:3992-2719|FD-Score:-3.54|P-value:2.94E-4 ID:SGTC_691|Compound:1548-0686|FD-Score:-3.74|P-value:1.41E-4 ID:SGTC_700|Compound:3453-2283|FD-Score:3.42|P-value:4.19E-4 ID:SGTC_972|Compound:1319-0219|FD-Score:-3.19|P-value:9.72E-4 ID:SGTC_978|Compound:1416-0512|FD-Score:4.89|P-value:8.91E-7 ID:SGTC_980|Compound:1473-0395|FD-Score:3.58|P-value:2.33E-4 ID:SGTC_987|Compound:1497-1013|FD-Score:-3.83|P-value:1.01E-4 ID:SGTC_1067|Compound:clotrimazole|FD-Score:3.41|P-value:4.35E-4 ID:SGTC_1068|Compound:loratadine|FD-Score:4.48|P-value:5.95E-6 ID:SGTC_1071|Compound:trifluoperazine|FD-Score:-3.30|P-value:6.81E-4 ID:SGTC_1076|Compound:itraconazole|FD-Score:3.96|P-value:5.53E-5 ID:SGTC_1203|Compound:3992-3522|FD-Score:5.54|P-value:3.16E-8 ID:SGTC_1206|Compound:3978-0017|FD-Score:4.32|P-value:1.23E-5 ID:SGTC_1252|Compound:0493-0875|FD-Score:6.41|P-value:1.94E-10 ID:SGTC_1281|Compound:0884-0031|FD-Score:3.55|P-value:2.64E-4 ID:SGTC_1322|Compound:1348-1070|FD-Score:3.35|P-value:5.31E-4 ID:SGTC_1350|Compound:1495-0454|FD-Score:-3.35|P-value:5.68E-4 ID:SGTC_1419|Compound:3993-2473|FD-Score:4.06|P-value:3.71E-5 ID:SGTC_1513|Compound:0337-0559|FD-Score:-3.23|P-value:8.66E-4 ID:SGTC_1515|Compound:3460-0031|FD-Score:3.26|P-value:7.08E-4 ID:SGTC_1525|Compound:8-methoxypsoralen|FD-Score:3.35|P-value:5.30E-4 ID:SGTC_1577|Compound:3(2'-chlorophenyl)-7-ethoxycoumarin|FD-Score:-3.27|P-value:7.44E-4 ID:SGTC_1586|Compound:deoxycorticosterone acetate|FD-Score:-3.61|P-value:2.25E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:3.57|P-value:2.45E-4 ID:SGTC_2022|Compound:5136654|FD-Score:-3.20|P-value:9.30E-4 ID:SGTC_2135|Compound:5326629|FD-Score:3.91|P-value:6.67E-5 ID:SGTC_2173|Compound:5763948|FD-Score:3.34|P-value:5.57E-4 ID:SGTC_2541|Compound:muurolladie-3-one|FD-Score:3.32|P-value:5.77E-4 ID:SGTC_2544|Compound:sphondin|FD-Score:4.16|P-value:2.38E-5 ID:SGTC_2780|Compound:5215298|FD-Score:4.97|P-value:5.99E-7 ID:SGTC_2832|Compound:7992762|FD-Score:-4.19|P-value:2.37E-5 ID:SGTC_2937|Compound:9044753|FD-Score:3.26|P-value:7.09E-4 ID:SGTC_2948|Compound:9070189|FD-Score:5.54|P-value:3.19E-8 ID:SGTC_2975|Compound:9084847|FD-Score:-3.47|P-value:3.74E-4 ID:SGTC_3142|Compound:9086498|FD-Score:4.34|P-value:1.15E-5 ID:SGTC_3154|Compound:9100113|FD-Score:4.77|P-value:1.59E-6 ID:SGTC_3229|Compound:9132204|FD-Score:3.58|P-value:2.38E-4 ID:SGTC_3234|Compound:9132280|FD-Score:4.80|P-value:1.41E-6 ID:SGTC_3348|Compound:9149464|FD-Score:-4.95|P-value:7.37E-7

Top fitness defect scores for YBR253W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1252 0493-0875 6.41 34.8 µM 1.94E-10 1 7.15E-11 1.03
2 SGTC_1203 3992-3522 5.54 103.0 µM 3.16E-8 3 1.50E-8 0.12
3 SGTC_2948 9070189 5.54 9.4 µM 3.19E-8 plasma membrane duress 4 1.52E-8 0.42
4 SGTC_2780 5215298 4.97 71.4 µM 5.99E-7 9 3.30E-7 0.05
5 SGTC_978 1416-0512 4.89 87.4 µM 8.91E-7 redox potentiating 4 5.00E-7 0.69
6 SGTC_3234 9132280 4.80 49.5 µM 1.41E-6 2 8.12E-7 0.67
7 SGTC_3154 9100113 4.77 49.5 µM 1.59E-6 5 9.18E-7 0.36
8 SGTC_512 prima-1 4.51 270.0 µM 5.22E-6 DNA intercalators 9 3.20E-6 0.16
9 SGTC_1068 loratadine 4.48 26.9 µM 5.95E-6 4 3.67E-6 0.52
10 SGTC_3142 9086498 4.34 49.5 µM 1.15E-5 iron homeostasis 38 7.28E-6 0.01
11 SGTC_1206 3978-0017 4.32 132.0 µM 1.23E-5 ERG2 4 7.84E-6 0.13
12 SGTC_2544 sphondin 4.16 68.5 µM 2.38E-5 RNA pol III & RNase P/MRP 36 1.57E-5 0.20
13 SGTC_264 promethazine 4.11 12.5 mM 2.97E-5 DNA intercalators 20 1.98E-5 0.16
14 SGTC_1419 3993-2473 4.06 62.6 µM 3.71E-5 3 2.49E-5 0.12
15 SGTC_1076 itraconazole 3.96 331.0 nM 5.53E-5 5 3.79E-5 0.33
16 SGTC_2135 5326629 3.91 150.4 µM 6.67E-5 RPP1 & pyrimidine depletion 5 4.62E-5 0.00
17 SGTC_81 0411-0046 3.75 10.0 µM 1.23E-4 40 8.80E-5 0.04
18 SGTC_980 1473-0395 3.58 201.0 µM 2.33E-4 9 1.71E-4 0.16
19 SGTC_3229 9132204 3.58 49.5 µM 2.38E-4 2 1.75E-4 0.61
20 SGTC_1785 bathophenanthroline 3.57 24.0 µM 2.45E-4 heme requiring 30 1.80E-4 0.00

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.113 5.79E-11 YDR164C SEC1 het Sm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function
0.097 1.78E-8 YLR419W_p YLR419W_p hom Putative helicase with limited sequence similarity to human Rb protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR419W is not an essential gene
0.095 3.21E-8 YMR168C CEP3 het Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
0.095 3.23E-8 YDR041W RSM10 het Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S10 ribosomal protein; essential for viability, unlike most other mitoribosomal proteins
0.093 7.35E-8 YCL056C PEX34 hom Peroxisomal integral membrane protein that regulates peroxisome populations; interacts with Pex11p, Pex25p, and Pex27p to control both constitutive peroxisome division and peroxisome morphology and abundance during peroxisome proliferation
0.091 1.54E-7 YMR289W ABZ2 hom Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis
0.086 6.50E-7 YHL039W EFM1 hom Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.083 1.41E-6 YGR206W MVB12 hom ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin
0.082 2.11E-6 YEL032W MCM3 het Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex
0.081 2.45E-6 YGL118C_d YGL118C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.081 2.83E-6 YER049W TPA1 hom Poly(rA)-binding protein involved in translation termination efficiency, mRNA poly(A) tail length and mRNA stability; interacts with Sup45p (eRF1), Sup35p (eRF3) and Pab1p; similar to prolyl 4-hydroxylases; binds Fe(III) and 2-oxoglutarate
0.080 3.44E-6 YER149C PEA2 hom Coiled-coil polarisome protein required for polarized morphogenesis, cell fusion, and low affinity Ca2+ influx; forms polarisome complex with Bni1p, Bud6p, and Spa2p; localizes to sites of polarized growth
0.080 3.83E-6 YBR170C NPL4 hom Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; assists Cdc48p in the dislocation of misfolded, polyubiquitinated ERAD substrates that are subsequently delivered to the proteasome for degradation; also involved in the regulated destruction of resident ER membrane proteins, such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing (RUP)
0.079 4.33E-6 YHR159W_p TDA11_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele
0.079 4.62E-6 YMR260C TIF11 het Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3