YBR254C / TRS20

One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; mutations in the human homolog cause the spondyloepiphyseal dysplasia tarda (SEDL) disorder

Zygosity: Heterozygous strain
fixedexpanded
Profile for YBR254C / TRS20

Click on Significant Values for Screen Details ID:SGTC_16|Compound:erodoxin|FD-Score:3.07|P-value:7.61E-4 ID:SGTC_149|Compound:3970-0822|FD-Score:3.53|P-value:1.37E-4 ID:SGTC_172|Compound:1112-0046|FD-Score:-4.58|P-value:1.08E-6 ID:SGTC_256|Compound:clotrimazole|FD-Score:-4.88|P-value:2.22E-7 ID:SGTC_369|Compound:0957-0393|FD-Score:3.27|P-value:3.74E-4 ID:SGTC_371|Compound:0195-0006|FD-Score:4.84|P-value:2.89E-7 ID:SGTC_468|Compound:gf-109203x|FD-Score:-3.12|P-value:6.29E-4 ID:SGTC_635|Compound:k038-0028|FD-Score:4.00|P-value:1.77E-5 ID:SGTC_700|Compound:3453-2283|FD-Score:3.07|P-value:7.55E-4 ID:SGTC_729|Compound:4092-0585|FD-Score:4.29|P-value:4.73E-6 ID:SGTC_737|Compound:1950-0117|FD-Score:3.47|P-value:1.67E-4 ID:SGTC_873|Compound:0591-4978|FD-Score:-2.99|P-value:9.81E-4 ID:SGTC_878|Compound:0828-0289|FD-Score:3.96|P-value:2.21E-5 ID:SGTC_954|Compound:1189-0853|FD-Score:3.49|P-value:1.60E-4 ID:SGTC_991|Compound:3996-0057|FD-Score:-3.11|P-value:6.43E-4 ID:SGTC_1005|Compound:4092-0690|FD-Score:4.46|P-value:2.08E-6 ID:SGTC_1119|Compound:parthenolide|FD-Score:4.72|P-value:5.51E-7 ID:SGTC_1263|Compound:0774-1208|FD-Score:3.74|P-value:5.68E-5 ID:SGTC_1284|Compound:0929-0043|FD-Score:3.30|P-value:3.31E-4 ID:SGTC_1373|Compound:2887-0003|FD-Score:3.31|P-value:3.11E-4 ID:SGTC_1381|Compound:2324-0066|FD-Score:3.63|P-value:8.87E-5 ID:SGTC_1424|Compound:4048-1941|FD-Score:3.25|P-value:3.98E-4 ID:SGTC_1579|Compound:chlormadinone acetate|FD-Score:4.26|P-value:5.55E-6 ID:SGTC_1580|Compound:hydrocortisone 21-hemisuccinate|FD-Score:4.53|P-value:1.42E-6 ID:SGTC_1585|Compound:2',4'-dihydroxychalcone|FD-Score:-4.06|P-value:1.33E-5 ID:SGTC_1667|Compound:st014849|FD-Score:3.07|P-value:7.74E-4 ID:SGTC_1726|Compound:st035556|FD-Score:3.86|P-value:3.42E-5 ID:SGTC_1818|Compound:st052136|FD-Score:4.00|P-value:1.79E-5 ID:SGTC_1868|Compound:st057644|FD-Score:5.03|P-value:1.02E-7 ID:SGTC_1895|Compound:st058434|FD-Score:3.88|P-value:3.09E-5 ID:SGTC_2324|Compound:6149169|FD-Score:3.15|P-value:5.66E-4 ID:SGTC_2329|Compound:6138490|FD-Score:3.74|P-value:5.68E-5 ID:SGTC_2333|Compound:9017922|FD-Score:-3.03|P-value:8.49E-4 ID:SGTC_2349|Compound:9023730|FD-Score:-3.03|P-value:8.57E-4 ID:SGTC_2385|Compound:9074692|FD-Score:3.73|P-value:5.80E-5 ID:SGTC_2398|Compound:5228213|FD-Score:5.86|P-value:6.90E-10 ID:SGTC_2399|Compound:5884133|FD-Score:3.45|P-value:1.86E-4 ID:SGTC_2400|Compound:5203494|FD-Score:6.24|P-value:5.81E-11 ID:SGTC_2413|Compound:inermin|FD-Score:6.45|P-value:1.38E-11 ID:SGTC_2422|Compound:chlorphenesin carbamate|FD-Score:-4.32|P-value:3.92E-6 ID:SGTC_2533|Compound:agelasine|FD-Score:3.04|P-value:8.62E-4 ID:SGTC_2537|Compound:larixol|FD-Score:-3.33|P-value:2.86E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:4.11|P-value:1.11E-5 ID:SGTC_2709|Compound:st078560|FD-Score:-3.13|P-value:5.94E-4 ID:SGTC_2758|Compound:dehydroepiandrosterone|FD-Score:3.33|P-value:2.90E-4 ID:SGTC_2794|Compound:7785382|FD-Score:-3.96|P-value:2.15E-5 ID:SGTC_2892|Compound:9047358|FD-Score:3.33|P-value:2.95E-4 ID:SGTC_2945|Compound:9067681|FD-Score:-3.04|P-value:8.40E-4 ID:SGTC_3077|Compound:9117319|FD-Score:3.53|P-value:1.36E-4 ID:SGTC_3080|Compound:9117754|FD-Score:-3.69|P-value:6.74E-5 ID:SGTC_3126|Compound:9127922|FD-Score:4.76|P-value:4.49E-7 ID:SGTC_3210|Compound:9128157|FD-Score:5.10|P-value:6.81E-8 ID:SGTC_3311|Compound:9131146|FD-Score:-3.18|P-value:5.01E-4 ID:SGTC_16|Compound:erodoxin|FD-Score:3.07|P-value:7.61E-4 ID:SGTC_149|Compound:3970-0822|FD-Score:3.53|P-value:1.37E-4 ID:SGTC_172|Compound:1112-0046|FD-Score:-4.58|P-value:1.08E-6 ID:SGTC_256|Compound:clotrimazole|FD-Score:-4.88|P-value:2.22E-7 ID:SGTC_369|Compound:0957-0393|FD-Score:3.27|P-value:3.74E-4 ID:SGTC_371|Compound:0195-0006|FD-Score:4.84|P-value:2.89E-7 ID:SGTC_468|Compound:gf-109203x|FD-Score:-3.12|P-value:6.29E-4 ID:SGTC_635|Compound:k038-0028|FD-Score:4.00|P-value:1.77E-5 ID:SGTC_700|Compound:3453-2283|FD-Score:3.07|P-value:7.55E-4 ID:SGTC_729|Compound:4092-0585|FD-Score:4.29|P-value:4.73E-6 ID:SGTC_737|Compound:1950-0117|FD-Score:3.47|P-value:1.67E-4 ID:SGTC_873|Compound:0591-4978|FD-Score:-2.99|P-value:9.81E-4 ID:SGTC_878|Compound:0828-0289|FD-Score:3.96|P-value:2.21E-5 ID:SGTC_954|Compound:1189-0853|FD-Score:3.49|P-value:1.60E-4 ID:SGTC_991|Compound:3996-0057|FD-Score:-3.11|P-value:6.43E-4 ID:SGTC_1005|Compound:4092-0690|FD-Score:4.46|P-value:2.08E-6 ID:SGTC_1119|Compound:parthenolide|FD-Score:4.72|P-value:5.51E-7 ID:SGTC_1263|Compound:0774-1208|FD-Score:3.74|P-value:5.68E-5 ID:SGTC_1284|Compound:0929-0043|FD-Score:3.30|P-value:3.31E-4 ID:SGTC_1373|Compound:2887-0003|FD-Score:3.31|P-value:3.11E-4 ID:SGTC_1381|Compound:2324-0066|FD-Score:3.63|P-value:8.87E-5 ID:SGTC_1424|Compound:4048-1941|FD-Score:3.25|P-value:3.98E-4 ID:SGTC_1579|Compound:chlormadinone acetate|FD-Score:4.26|P-value:5.55E-6 ID:SGTC_1580|Compound:hydrocortisone 21-hemisuccinate|FD-Score:4.53|P-value:1.42E-6 ID:SGTC_1585|Compound:2',4'-dihydroxychalcone|FD-Score:-4.06|P-value:1.33E-5 ID:SGTC_1667|Compound:st014849|FD-Score:3.07|P-value:7.74E-4 ID:SGTC_1726|Compound:st035556|FD-Score:3.86|P-value:3.42E-5 ID:SGTC_1818|Compound:st052136|FD-Score:4.00|P-value:1.79E-5 ID:SGTC_1868|Compound:st057644|FD-Score:5.03|P-value:1.02E-7 ID:SGTC_1895|Compound:st058434|FD-Score:3.88|P-value:3.09E-5 ID:SGTC_2324|Compound:6149169|FD-Score:3.15|P-value:5.66E-4 ID:SGTC_2329|Compound:6138490|FD-Score:3.74|P-value:5.68E-5 ID:SGTC_2333|Compound:9017922|FD-Score:-3.03|P-value:8.49E-4 ID:SGTC_2349|Compound:9023730|FD-Score:-3.03|P-value:8.57E-4 ID:SGTC_2385|Compound:9074692|FD-Score:3.73|P-value:5.80E-5 ID:SGTC_2398|Compound:5228213|FD-Score:5.86|P-value:6.90E-10 ID:SGTC_2399|Compound:5884133|FD-Score:3.45|P-value:1.86E-4 ID:SGTC_2400|Compound:5203494|FD-Score:6.24|P-value:5.81E-11 ID:SGTC_2413|Compound:inermin|FD-Score:6.45|P-value:1.38E-11 ID:SGTC_2422|Compound:chlorphenesin carbamate|FD-Score:-4.32|P-value:3.92E-6 ID:SGTC_2533|Compound:agelasine|FD-Score:3.04|P-value:8.62E-4 ID:SGTC_2537|Compound:larixol|FD-Score:-3.33|P-value:2.86E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:4.11|P-value:1.11E-5 ID:SGTC_2709|Compound:st078560|FD-Score:-3.13|P-value:5.94E-4 ID:SGTC_2758|Compound:dehydroepiandrosterone|FD-Score:3.33|P-value:2.90E-4 ID:SGTC_2794|Compound:7785382|FD-Score:-3.96|P-value:2.15E-5 ID:SGTC_2892|Compound:9047358|FD-Score:3.33|P-value:2.95E-4 ID:SGTC_2945|Compound:9067681|FD-Score:-3.04|P-value:8.40E-4 ID:SGTC_3077|Compound:9117319|FD-Score:3.53|P-value:1.36E-4 ID:SGTC_3080|Compound:9117754|FD-Score:-3.69|P-value:6.74E-5 ID:SGTC_3126|Compound:9127922|FD-Score:4.76|P-value:4.49E-7 ID:SGTC_3210|Compound:9128157|FD-Score:5.10|P-value:6.81E-8 ID:SGTC_3311|Compound:9131146|FD-Score:-3.18|P-value:5.01E-4

Top fitness defect scores for YBR254C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2413 inermin 6.45 26.1 µM 1.38E-11 8 5.74E-11 0.48
2 SGTC_2400 5203494 6.24 178.7 µM 5.81E-11 RSC complex & mRNA processing 8 2.20E-10 0.32
3 SGTC_2398 5228213 5.86 48.8 µM 6.90E-10 iron homeostasis 6 2.25E-9 0.77
4 SGTC_3210 9128157 5.10 49.5 µM 6.81E-8 3 1.67E-7 0.27
5 SGTC_1868 st057644 5.03 42.7 µM 1.02E-7 5 2.44E-7 0.91
6 SGTC_371 0195-0006 4.84 7.4 µM 2.89E-7 6 6.49E-7 0.06
7 SGTC_3126 9127922 4.76 49.5 µM 4.49E-7 fatty acid desaturase (OLE1) 20 9.82E-7 0.13
8 SGTC_1119 parthenolide 4.72 318.5 nM 5.51E-7 TSC3-RPN4 5 1.19E-6 0.04
9 SGTC_1580 hydrocortisone 21-hemisuccinate 4.53 43.2 µM 1.42E-6 2 2.90E-6 0.43
10 SGTC_1005 4092-0690 4.46 4.4 µM 2.08E-6 plasma membrane duress 5 4.15E-6 0.04
11 SGTC_729 4092-0585 4.29 94.1 µM 4.73E-6 1 8.97E-6 0.54
12 SGTC_1579 chlormadinone acetate 4.26 49.4 µM 5.55E-6 5 1.04E-5 0.38
13 SGTC_2679 rapamycin 4.11 1.0 nM 1.11E-5 50 2.00E-5 0.06
14 SGTC_635 k038-0028 4.00 24.7 µM 1.77E-5 ubiquinone biosynthesis & proteasome 16 3.11E-5 0.09
15 SGTC_1818 st052136 4.00 56.3 µM 1.79E-5 NEO1-PIK1 14 3.13E-5 0.03
16 SGTC_878 0828-0289 3.96 13.7 µM 2.21E-5 21 3.82E-5 0.24
17 SGTC_1895 st058434 3.88 18.3 µM 3.09E-5 4 5.23E-5 0.20
18 SGTC_1726 st035556 3.86 14.6 µM 3.42E-5 4 5.76E-5 0.28
19 SGTC_1263 0774-1208 3.74 64.8 µM 5.68E-5 5 9.28E-5 0.05
20 SGTC_2329 6138490 3.74 200.0 µM 5.68E-5 16 9.28E-5 0.30

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.212 2.44E-35 YFL038C YPT1 het Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p)
0.135 4.00E-15 YOL146W PSF3 het Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
0.133 1.20E-14 YOR246C ENV9 hom Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts
0.123 7.44E-13 YHR086W NAM8 hom RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of REC107 pre-mRNA; Nam8p regulon embraces the meiotic pre-mRNAs of REC107, HFM1, AMA1 SPO22 and PCH2; the putative RNA binding domains RRM2 and RRM3 are required for Nam8p meiotic function
0.115 2.11E-11 YBR243C ALG7 het UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin
0.108 3.32E-10 YDR088C SLU7 het RNA splicing factor, required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain
0.102 3.23E-9 YHR101C BIG1 het Integral membrane protein of the endoplasmic reticulum, required for normal content of cell wall beta-1,6-glucan
0.097 1.94E-8 YDL096C_d OPI6_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential
0.092 9.67E-8 YDR057W YOS9 hom ER quality-control lectin; integral subunit of the HRD ligase; binds to glycans with terminal alpha-1,6 linked mannose on misfolded N-glycosylated proteins and participates in targeting proteins to ERAD; member of the OS-9 protein family
0.091 1.45E-7 YHR187W IKI1 hom Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin
0.090 1.76E-7 YGL113W SLD3 het Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress
0.089 2.37E-7 YDR023W SES1 het Cytosolic seryl-tRNA synthetase, class II aminoacyl-tRNA synthetase that aminoacylates tRNA(Ser), displays tRNA-dependent amino acid recognition which enhances discrimination of the serine substrate, interacts with peroxin Pex21p
0.087 3.87E-7 YMR183C SSO2 hom Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane; syntaxin homolog that is functionally redundant with Sso1p
0.086 6.95E-7 YLL035W GRC3 het Polynucleotide kinase present on rDNA that is required for efficient transcription termination by RNA polymerase I; required for cell growth; mRNA is cell-cycle regulated
0.084 9.87E-7 YDR304C CPR5 hom Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the endoplasmic reticulum, catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; transcriptionally induced in response to unfolded proteins in the ER