YBR257W / POP4

Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P

Zygosity: Heterozygous strain
fixedexpanded
Profile for YBR257W / POP4

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:3.74|P-value:1.65E-4 ID:SGTC_18|Compound:0111-0177|FD-Score:4.26|P-value:2.15E-5 ID:SGTC_21|Compound:0849-0113|FD-Score:6.82|P-value:2.62E-11 ID:SGTC_147|Compound:4466-0538|FD-Score:4.60|P-value:4.86E-6 ID:SGTC_184|Compound:1494-0393|FD-Score:5.80|P-value:1.21E-8 ID:SGTC_202|Compound:3594-0513|FD-Score:6.94|P-value:1.16E-11 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:3.33|P-value:7.13E-4 ID:SGTC_303|Compound:1348-1668|FD-Score:4.10|P-value:4.06E-5 ID:SGTC_539|Compound:4859-0462|FD-Score:16.80|P-value:1.41E-59 ID:SGTC_572|Compound:0960-0087|FD-Score:3.69|P-value:1.98E-4 ID:SGTC_573|Compound:3702-0553|FD-Score:3.58|P-value:2.96E-4 ID:SGTC_580|Compound:1123-0028|FD-Score:4.13|P-value:3.59E-5 ID:SGTC_612|Compound:0302-0167|FD-Score:3.54|P-value:3.39E-4 ID:SGTC_667|Compound:1498-1044|FD-Score:3.28|P-value:8.47E-4 ID:SGTC_720|Compound:r052-1644|FD-Score:4.04|P-value:5.28E-5 ID:SGTC_799|Compound:1310-0557|FD-Score:3.34|P-value:6.91E-4 ID:SGTC_919|Compound:tnp00227|FD-Score:6.72|P-value:4.87E-11 ID:SGTC_1016|Compound:4226-1401|FD-Score:13.10|P-value:3.95E-37 ID:SGTC_1129|Compound:3453-1384|FD-Score:3.68|P-value:2.07E-4 ID:SGTC_1166|Compound:k206-0078|FD-Score:3.76|P-value:1.56E-4 ID:SGTC_1181|Compound:1222-0449|FD-Score:3.40|P-value:5.55E-4 ID:SGTC_1399|Compound:3562-5772|FD-Score:8.77|P-value:1.47E-17 ID:SGTC_1416|Compound:3966-0321|FD-Score:3.74|P-value:1.67E-4 ID:SGTC_1534|Compound:6-azauridine|FD-Score:4.31|P-value:1.73E-5 ID:SGTC_1643|Compound:st009866|FD-Score:15.50|P-value:5.66E-51 ID:SGTC_1872|Compound:amphotericin b|FD-Score:17.60|P-value:5.43E-65 ID:SGTC_2057|Compound:5236571|FD-Score:25.70|P-value:6.71E-136 ID:SGTC_2090|Compound:5301565|FD-Score:3.58|P-value:2.97E-4 ID:SGTC_2110|Compound:5483698|FD-Score:3.48|P-value:4.16E-4 ID:SGTC_2156|Compound:5649887|FD-Score:26.20|P-value:1.82E-141 ID:SGTC_2162|Compound:5663267|FD-Score:4.36|P-value:1.40E-5 ID:SGTC_2274|Compound:7947094|FD-Score:7.12|P-value:3.64E-12 ID:SGTC_2320|Compound:7373003|FD-Score:3.23|P-value:9.78E-4 ID:SGTC_2329|Compound:6138490|FD-Score:9.44|P-value:4.49E-20 ID:SGTC_2330|Compound:7979373|FD-Score:-5.33|P-value:1.11E-7 ID:SGTC_2332|Compound:7982454|FD-Score:4.65|P-value:3.95E-6 ID:SGTC_2360|Compound:9040895|FD-Score:-3.43|P-value:4.08E-4 ID:SGTC_2362|Compound:9071156|FD-Score:-4.10|P-value:3.28E-5 ID:SGTC_2385|Compound:9074692|FD-Score:4.04|P-value:5.28E-5 ID:SGTC_2411|Compound:st077133|FD-Score:3.83|P-value:1.18E-4 ID:SGTC_2458|Compound:5808907|FD-Score:-4.68|P-value:2.68E-6 ID:SGTC_2461|Compound:5755302|FD-Score:-3.46|P-value:3.76E-4 ID:SGTC_2470|Compound:5664817|FD-Score:3.78|P-value:1.45E-4 ID:SGTC_2474|Compound:5321570|FD-Score:4.56|P-value:6.01E-6 ID:SGTC_2507|Compound:purpurin|FD-Score:20.50|P-value:2.35E-87 ID:SGTC_2509|Compound:methyl 7-deshydroxypyrogallin-4-carboxylate|FD-Score:14.40|P-value:1.99E-44 ID:SGTC_2579|Compound:2',5'-dihydroxy-4-methoxychalcone|FD-Score:5.36|P-value:1.26E-7 ID:SGTC_2605|Compound:digitonin|FD-Score:-4.22|P-value:1.95E-5 ID:SGTC_2608|Compound:thymoquinone|FD-Score:-4.89|P-value:9.85E-7 ID:SGTC_2612|Compound:dictamnine|FD-Score:15.50|P-value:9.69E-51 ID:SGTC_3008|Compound:9078912|FD-Score:4.11|P-value:4.00E-5 ID:SGTC_3214|Compound:9129694|FD-Score:-3.45|P-value:3.82E-4 ID:SGTC_3215|Compound:9129942|FD-Score:-4.95|P-value:7.36E-7 ID:SGTC_3221|Compound:9128209|FD-Score:3.66|P-value:2.22E-4 ID:SGTC_6|Compound:0844-0013|FD-Score:3.74|P-value:1.65E-4 ID:SGTC_18|Compound:0111-0177|FD-Score:4.26|P-value:2.15E-5 ID:SGTC_21|Compound:0849-0113|FD-Score:6.82|P-value:2.62E-11 ID:SGTC_147|Compound:4466-0538|FD-Score:4.60|P-value:4.86E-6 ID:SGTC_184|Compound:1494-0393|FD-Score:5.80|P-value:1.21E-8 ID:SGTC_202|Compound:3594-0513|FD-Score:6.94|P-value:1.16E-11 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:3.33|P-value:7.13E-4 ID:SGTC_303|Compound:1348-1668|FD-Score:4.10|P-value:4.06E-5 ID:SGTC_539|Compound:4859-0462|FD-Score:16.80|P-value:1.41E-59 ID:SGTC_572|Compound:0960-0087|FD-Score:3.69|P-value:1.98E-4 ID:SGTC_573|Compound:3702-0553|FD-Score:3.58|P-value:2.96E-4 ID:SGTC_580|Compound:1123-0028|FD-Score:4.13|P-value:3.59E-5 ID:SGTC_612|Compound:0302-0167|FD-Score:3.54|P-value:3.39E-4 ID:SGTC_667|Compound:1498-1044|FD-Score:3.28|P-value:8.47E-4 ID:SGTC_720|Compound:r052-1644|FD-Score:4.04|P-value:5.28E-5 ID:SGTC_799|Compound:1310-0557|FD-Score:3.34|P-value:6.91E-4 ID:SGTC_919|Compound:tnp00227|FD-Score:6.72|P-value:4.87E-11 ID:SGTC_1016|Compound:4226-1401|FD-Score:13.10|P-value:3.95E-37 ID:SGTC_1129|Compound:3453-1384|FD-Score:3.68|P-value:2.07E-4 ID:SGTC_1166|Compound:k206-0078|FD-Score:3.76|P-value:1.56E-4 ID:SGTC_1181|Compound:1222-0449|FD-Score:3.40|P-value:5.55E-4 ID:SGTC_1399|Compound:3562-5772|FD-Score:8.77|P-value:1.47E-17 ID:SGTC_1416|Compound:3966-0321|FD-Score:3.74|P-value:1.67E-4 ID:SGTC_1534|Compound:6-azauridine|FD-Score:4.31|P-value:1.73E-5 ID:SGTC_1643|Compound:st009866|FD-Score:15.50|P-value:5.66E-51 ID:SGTC_1872|Compound:amphotericin b|FD-Score:17.60|P-value:5.43E-65 ID:SGTC_2057|Compound:5236571|FD-Score:25.70|P-value:6.71E-136 ID:SGTC_2090|Compound:5301565|FD-Score:3.58|P-value:2.97E-4 ID:SGTC_2110|Compound:5483698|FD-Score:3.48|P-value:4.16E-4 ID:SGTC_2156|Compound:5649887|FD-Score:26.20|P-value:1.82E-141 ID:SGTC_2162|Compound:5663267|FD-Score:4.36|P-value:1.40E-5 ID:SGTC_2274|Compound:7947094|FD-Score:7.12|P-value:3.64E-12 ID:SGTC_2320|Compound:7373003|FD-Score:3.23|P-value:9.78E-4 ID:SGTC_2329|Compound:6138490|FD-Score:9.44|P-value:4.49E-20 ID:SGTC_2330|Compound:7979373|FD-Score:-5.33|P-value:1.11E-7 ID:SGTC_2332|Compound:7982454|FD-Score:4.65|P-value:3.95E-6 ID:SGTC_2360|Compound:9040895|FD-Score:-3.43|P-value:4.08E-4 ID:SGTC_2362|Compound:9071156|FD-Score:-4.10|P-value:3.28E-5 ID:SGTC_2385|Compound:9074692|FD-Score:4.04|P-value:5.28E-5 ID:SGTC_2411|Compound:st077133|FD-Score:3.83|P-value:1.18E-4 ID:SGTC_2458|Compound:5808907|FD-Score:-4.68|P-value:2.68E-6 ID:SGTC_2461|Compound:5755302|FD-Score:-3.46|P-value:3.76E-4 ID:SGTC_2470|Compound:5664817|FD-Score:3.78|P-value:1.45E-4 ID:SGTC_2474|Compound:5321570|FD-Score:4.56|P-value:6.01E-6 ID:SGTC_2507|Compound:purpurin|FD-Score:20.50|P-value:2.35E-87 ID:SGTC_2509|Compound:methyl 7-deshydroxypyrogallin-4-carboxylate|FD-Score:14.40|P-value:1.99E-44 ID:SGTC_2579|Compound:2',5'-dihydroxy-4-methoxychalcone|FD-Score:5.36|P-value:1.26E-7 ID:SGTC_2605|Compound:digitonin|FD-Score:-4.22|P-value:1.95E-5 ID:SGTC_2608|Compound:thymoquinone|FD-Score:-4.89|P-value:9.85E-7 ID:SGTC_2612|Compound:dictamnine|FD-Score:15.50|P-value:9.69E-51 ID:SGTC_3008|Compound:9078912|FD-Score:4.11|P-value:4.00E-5 ID:SGTC_3214|Compound:9129694|FD-Score:-3.45|P-value:3.82E-4 ID:SGTC_3215|Compound:9129942|FD-Score:-4.95|P-value:7.36E-7 ID:SGTC_3221|Compound:9128209|FD-Score:3.66|P-value:2.22E-4

Top fitness defect scores for YBR257W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2156 5649887 26.20 115.0 µM 1.82E-141 RPP1 & pyrimidine depletion 2 1.86E-151 11.50
2 SGTC_2057 5236571 25.70 38.3 µM 6.71E-136 RPP1 & pyrimidine depletion 1 1.69E-145 9.23
3 SGTC_2507 purpurin 20.50 79.7 µM 2.35E-87 copper-dependent oxidative stress 1 1.59E-93 7.18
4 SGTC_1872 amphotericin b 17.60 148.0 nM 5.43E-65 RNA pol III & RNase P/MRP 2 1.41E-69 0.33
5 SGTC_539 4859-0462 16.80 2.5 µM 1.41E-59 RNA pol III & RNase P/MRP 2 8.89E-64 6.29
6 SGTC_1643 st009866 15.50 20.2 µM 5.66E-51 2 1.45E-54 2.64
7 SGTC_2612 dictamnine 15.50 15.5 µM 9.69E-51 RNA pol III & RNase P/MRP 4 2.59E-54 0.00
8 SGTC_2509 methyl 7-deshydroxypyrogallin-4-carboxylate 14.40 54.6 µM 1.99E-44 RPP1 & pyrimidine depletion 3 1.50E-47 6.41
9 SGTC_1016 4226-1401 13.10 38.0 µM 3.95E-37 RNA pol III & RNase P/MRP 2 9.82E-40 3.43
10 SGTC_2329 6138490 9.44 200.0 µM 4.49E-20 2 1.86E-21 3.92
11 SGTC_1399 3562-5772 8.77 25.0 µM 1.47E-17 RPP1 & pyrimidine depletion 5 9.22E-19 4.09
12 SGTC_2274 7947094 7.12 46.1 µM 3.64E-12 1 5.59E-13 1.98
13 SGTC_202 3594-0513 6.94 34.8 µM 1.16E-11 PDR1 3 1.94E-12 1.60
14 SGTC_21 0849-0113 6.82 23.0 µM 2.62E-11 1 4.65E-12 1.25
15 SGTC_919 tnp00227 6.72 403.6 nM 4.87E-11 RNA pol III & RNase P/MRP 3 9.03E-12 1.41
16 SGTC_184 1494-0393 5.80 24.7 µM 1.21E-8 10 3.34E-9 0.28
17 SGTC_2579 2',5'-dihydroxy-4-methoxychalcone 5.36 13.2 µM 1.26E-7 4 4.14E-8 0.06
18 SGTC_2332 7982454 4.65 138.9 µM 3.95E-6 7 1.66E-6 0.03
19 SGTC_147 4466-0538 4.60 29.2 µM 4.86E-6 ergosterol biosynthesis 2 2.08E-6 0.03
20 SGTC_2474 5321570 4.56 43.3 µM 6.01E-6 1 2.61E-6 0.44

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.462 2.18E-177 YKL052C ASK1 het Essential subunit of the Dam1 complex (aka DASH complex); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases; protein abundance increases in response to DNA replication stress
0.417 4.21E-141 YNL221C POP1 het Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
0.254 1.61E-50 YBR167C POP7 het Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
0.251 5.67E-49 YHR062C RPP1 het Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
0.209 1.75E-34 YJR007W SUI2 het Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress
0.200 1.81E-31 YDL150W RPC53 het RNA polymerase III subunit C53
0.181 4.78E-26 YLR291C GCD7 het Beta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
0.177 4.37E-25 YDL152W_d YDL152W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome
0.167 2.28E-22 YGR083C GCD2 het Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
0.162 2.80E-21 YNL282W POP3 het Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
0.161 6.66E-21 YNL236W SIN4 hom Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription
0.157 6.96E-20 YNR029C_p YNR029C_p hom Putative protein of unknown function, deletion confers reduced fitness in saline
0.139 5.29E-16 YOR260W GCD1 het Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
0.136 2.25E-15 YDR211W GCD6 het Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress
0.122 1.07E-12 YGR278W CWC22 het Spliceosome-associated protein that is required for pre-mRNA splicing; necessary for Prp2p function at the first catalytic step of splicing; has similarity to S. pombe Cwf22p; CWC22 is an essential protein