YBR267W / REI1

Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR267W / REI1

Click on Significant Values for Screen Details ID:SGTC_50|Compound:0929-0078|FD-Score:-4.16|P-value:6.98E-4 ID:SGTC_56|Compound:0957-0365|FD-Score:4.23|P-value:3.07E-4 ID:SGTC_59|Compound:0330-0160|FD-Score:4.90|P-value:3.87E-5 ID:SGTC_62|Compound:0180-0423|FD-Score:10.80|P-value:3.85E-18 ID:SGTC_97|Compound:0535-0701|FD-Score:7.28|P-value:2.70E-9 ID:SGTC_118|Compound:0083-0097|FD-Score:6.36|P-value:1.64E-7 ID:SGTC_159|Compound:3970-0959|FD-Score:5.76|P-value:1.84E-6 ID:SGTC_163|Compound:1327-0036|FD-Score:3.93|P-value:7.07E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:5.38|P-value:7.47E-6 ID:SGTC_214|Compound:4373-3496|FD-Score:4.55|P-value:1.18E-4 ID:SGTC_233|Compound:rapamycin|FD-Score:4.07|P-value:4.80E-4 ID:SGTC_316|Compound:k060-0003|FD-Score:4.40|P-value:1.85E-4 ID:SGTC_337|Compound:1315-0038|FD-Score:4.81|P-value:5.14E-5 ID:SGTC_543|Compound:3844-1095|FD-Score:5.50|P-value:4.85E-6 ID:SGTC_562|Compound:3772-2733|FD-Score:6.71|P-value:3.77E-8 ID:SGTC_623|Compound:k072-0108|FD-Score:4.22|P-value:3.18E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:3.90|P-value:7.76E-4 ID:SGTC_728|Compound:3030-5151|FD-Score:5.56|P-value:3.79E-6 ID:SGTC_800|Compound:3448-7362|FD-Score:6.24|P-value:2.67E-7 ID:SGTC_832|Compound:4083-0019|FD-Score:4.30|P-value:2.55E-4 ID:SGTC_839|Compound:0122-0028|FD-Score:5.23|P-value:1.27E-5 ID:SGTC_859|Compound:0439-0102|FD-Score:-4.49|P-value:2.77E-4 ID:SGTC_1031|Compound:k035-0009|FD-Score:5.58|P-value:3.59E-6 ID:SGTC_1073|Compound:triclabendazole|FD-Score:4.01|P-value:5.72E-4 ID:SGTC_1081|Compound:sertraline|FD-Score:3.82|P-value:9.73E-4 ID:SGTC_1103|Compound:duloxetine|FD-Score:4.47|P-value:1.49E-4 ID:SGTC_1129|Compound:3453-1384|FD-Score:6.03|P-value:6.32E-7 ID:SGTC_1140|Compound:3229-1848|FD-Score:4.03|P-value:5.47E-4 ID:SGTC_1144|Compound:3486-0183|FD-Score:3.88|P-value:8.18E-4 ID:SGTC_1375|Compound:2910-0797|FD-Score:4.18|P-value:3.55E-4 ID:SGTC_1380|Compound:2291-0730|FD-Score:4.18|P-value:3.51E-4 ID:SGTC_1407|Compound:3909-7909|FD-Score:5.50|P-value:4.71E-6 ID:SGTC_1492|Compound:4158-0558|FD-Score:4.67|P-value:8.04E-5 ID:SGTC_1727|Compound:st034309|FD-Score:5.78|P-value:1.65E-6 ID:SGTC_1733|Compound:st037357|FD-Score:5.92|P-value:9.77E-7 ID:SGTC_1750|Compound:st081859|FD-Score:4.38|P-value:1.97E-4 ID:SGTC_1775|Compound:st081588|FD-Score:4.17|P-value:3.61E-4 ID:SGTC_1827|Compound:berberine|FD-Score:7.28|P-value:2.78E-9 ID:SGTC_1829|Compound:berberine|FD-Score:5.84|P-value:1.34E-6 ID:SGTC_1836|Compound:st055642|FD-Score:5.89|P-value:1.11E-6 ID:SGTC_1842|Compound:st055362|FD-Score:-4.91|P-value:7.49E-5 ID:SGTC_1920|Compound:st075728|FD-Score:3.92|P-value:7.36E-4 ID:SGTC_1934|Compound:st074720|FD-Score:4.23|P-value:3.04E-4 ID:SGTC_2328|Compound:6201408|FD-Score:-4.47|P-value:2.92E-4 ID:SGTC_2347|Compound:9049783|FD-Score:4.05|P-value:5.11E-4 ID:SGTC_2409|Compound:alverine citrate|FD-Score:-4.29|P-value:4.82E-4 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:5.33|P-value:8.81E-6 ID:SGTC_2633|Compound:5,7-dihydroxyisoflavone|FD-Score:-4.42|P-value:3.36E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:5.57|P-value:3.75E-6 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.96|P-value:3.18E-5 ID:SGTC_2809|Compound:7964133|FD-Score:4.05|P-value:5.16E-4 ID:SGTC_2983|Compound:9022671|FD-Score:4.29|P-value:2.57E-4 ID:SGTC_3027|Compound:9089541|FD-Score:4.20|P-value:3.38E-4 ID:SGTC_3078|Compound:9116413|FD-Score:-4.40|P-value:3.57E-4 ID:SGTC_3101|Compound:9120531|FD-Score:3.94|P-value:7.06E-4 ID:SGTC_50|Compound:0929-0078|FD-Score:-4.16|P-value:6.98E-4 ID:SGTC_56|Compound:0957-0365|FD-Score:4.23|P-value:3.07E-4 ID:SGTC_59|Compound:0330-0160|FD-Score:4.90|P-value:3.87E-5 ID:SGTC_62|Compound:0180-0423|FD-Score:10.80|P-value:3.85E-18 ID:SGTC_97|Compound:0535-0701|FD-Score:7.28|P-value:2.70E-9 ID:SGTC_118|Compound:0083-0097|FD-Score:6.36|P-value:1.64E-7 ID:SGTC_159|Compound:3970-0959|FD-Score:5.76|P-value:1.84E-6 ID:SGTC_163|Compound:1327-0036|FD-Score:3.93|P-value:7.07E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:5.38|P-value:7.47E-6 ID:SGTC_214|Compound:4373-3496|FD-Score:4.55|P-value:1.18E-4 ID:SGTC_233|Compound:rapamycin|FD-Score:4.07|P-value:4.80E-4 ID:SGTC_316|Compound:k060-0003|FD-Score:4.40|P-value:1.85E-4 ID:SGTC_337|Compound:1315-0038|FD-Score:4.81|P-value:5.14E-5 ID:SGTC_543|Compound:3844-1095|FD-Score:5.50|P-value:4.85E-6 ID:SGTC_562|Compound:3772-2733|FD-Score:6.71|P-value:3.77E-8 ID:SGTC_623|Compound:k072-0108|FD-Score:4.22|P-value:3.18E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:3.90|P-value:7.76E-4 ID:SGTC_728|Compound:3030-5151|FD-Score:5.56|P-value:3.79E-6 ID:SGTC_800|Compound:3448-7362|FD-Score:6.24|P-value:2.67E-7 ID:SGTC_832|Compound:4083-0019|FD-Score:4.30|P-value:2.55E-4 ID:SGTC_839|Compound:0122-0028|FD-Score:5.23|P-value:1.27E-5 ID:SGTC_859|Compound:0439-0102|FD-Score:-4.49|P-value:2.77E-4 ID:SGTC_1031|Compound:k035-0009|FD-Score:5.58|P-value:3.59E-6 ID:SGTC_1073|Compound:triclabendazole|FD-Score:4.01|P-value:5.72E-4 ID:SGTC_1081|Compound:sertraline|FD-Score:3.82|P-value:9.73E-4 ID:SGTC_1103|Compound:duloxetine|FD-Score:4.47|P-value:1.49E-4 ID:SGTC_1129|Compound:3453-1384|FD-Score:6.03|P-value:6.32E-7 ID:SGTC_1140|Compound:3229-1848|FD-Score:4.03|P-value:5.47E-4 ID:SGTC_1144|Compound:3486-0183|FD-Score:3.88|P-value:8.18E-4 ID:SGTC_1375|Compound:2910-0797|FD-Score:4.18|P-value:3.55E-4 ID:SGTC_1380|Compound:2291-0730|FD-Score:4.18|P-value:3.51E-4 ID:SGTC_1407|Compound:3909-7909|FD-Score:5.50|P-value:4.71E-6 ID:SGTC_1492|Compound:4158-0558|FD-Score:4.67|P-value:8.04E-5 ID:SGTC_1727|Compound:st034309|FD-Score:5.78|P-value:1.65E-6 ID:SGTC_1733|Compound:st037357|FD-Score:5.92|P-value:9.77E-7 ID:SGTC_1750|Compound:st081859|FD-Score:4.38|P-value:1.97E-4 ID:SGTC_1775|Compound:st081588|FD-Score:4.17|P-value:3.61E-4 ID:SGTC_1827|Compound:berberine|FD-Score:7.28|P-value:2.78E-9 ID:SGTC_1829|Compound:berberine|FD-Score:5.84|P-value:1.34E-6 ID:SGTC_1836|Compound:st055642|FD-Score:5.89|P-value:1.11E-6 ID:SGTC_1842|Compound:st055362|FD-Score:-4.91|P-value:7.49E-5 ID:SGTC_1920|Compound:st075728|FD-Score:3.92|P-value:7.36E-4 ID:SGTC_1934|Compound:st074720|FD-Score:4.23|P-value:3.04E-4 ID:SGTC_2328|Compound:6201408|FD-Score:-4.47|P-value:2.92E-4 ID:SGTC_2347|Compound:9049783|FD-Score:4.05|P-value:5.11E-4 ID:SGTC_2409|Compound:alverine citrate|FD-Score:-4.29|P-value:4.82E-4 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:5.33|P-value:8.81E-6 ID:SGTC_2633|Compound:5,7-dihydroxyisoflavone|FD-Score:-4.42|P-value:3.36E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:5.57|P-value:3.75E-6 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.96|P-value:3.18E-5 ID:SGTC_2809|Compound:7964133|FD-Score:4.05|P-value:5.16E-4 ID:SGTC_2983|Compound:9022671|FD-Score:4.29|P-value:2.57E-4 ID:SGTC_3027|Compound:9089541|FD-Score:4.20|P-value:3.38E-4 ID:SGTC_3078|Compound:9116413|FD-Score:-4.40|P-value:3.57E-4 ID:SGTC_3101|Compound:9120531|FD-Score:3.94|P-value:7.06E-4

Top fitness defect scores for YBR267W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_62 0180-0423 10.80 3.2 µM 3.85E-18 60S ribosome export 1 1.85E-27
2 SGTC_97 0535-0701 7.28 19.1 µM 2.70E-9 60S ribosome export 3 1.63E-13
3 SGTC_1827 berberine 7.28 7.2 µM 2.78E-9 60S ribosome export 1 1.70E-13
4 SGTC_562 3772-2733 6.71 112.0 µM 3.77E-8 ERG2 27 1.01E-11
5 SGTC_118 0083-0097 6.36 135.3 µM 1.64E-7 translation 14 1.00E-10
6 SGTC_800 3448-7362 6.24 155.0 µM 2.67E-7 translation 42 2.14E-10
7 SGTC_1129 3453-1384 6.03 223.0 µM 6.32E-7 10 8.17E-10
8 SGTC_1733 st037357 5.92 54.9 µM 9.77E-7 60S ribosome export 5 1.61E-9
9 SGTC_1836 st055642 5.89 81.0 µM 1.11E-6 4 1.97E-9
10 SGTC_1829 berberine 5.84 14.4 µM 1.34E-6 60S ribosome export 2 2.63E-9
11 SGTC_1727 st034309 5.78 9.9 µM 1.65E-6 8 3.64E-9
12 SGTC_159 3970-0959 5.76 50.0 µM 1.84E-6 29 4.29E-9
13 SGTC_1031 k035-0009 5.58 16.0 µM 3.59E-6 9 1.22E-8
14 SGTC_2651 1327-0036 5.57 5.0 µM 3.75E-6 87 1.30E-8
15 SGTC_728 3030-5151 5.56 22.5 µM 3.79E-6 3 1.32E-8
16 SGTC_1407 3909-7909 5.50 12.3 µM 4.71E-6 60S ribosome export 3 1.85E-8
17 SGTC_543 3844-1095 5.50 191.0 µM 4.85E-6 ERG2 21 1.94E-8
18 SGTC_168 3970-0862 5.38 530.5 µM 7.47E-6 61 3.79E-8
19 SGTC_2572 astragaloside iv 5.33 100.0 µM 8.81E-6 6 4.90E-8
20 SGTC_839 0122-0028 5.23 230.0 µM 1.27E-5 RSC complex & mRNA processing 11 8.68E-8

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.197 1.22E-30 YNL227C JJJ1 hom Co-chaperone that stimulates the ATPase activity of Ssa1p, required for a late step of ribosome biogenesis; associated with the cytosolic large ribosomal subunit; contains a J-domain; mutation causes defects in fluid-phase endocytosis
0.195 4.47E-30 YML073C RPL6A hom Ribosomal 60S subunit protein L6A; N-terminally acetylated; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6A has a paralog, RPL6B, that arose from the whole genome duplication
0.193 1.91E-29 YDR083W RRP8 hom Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1
0.186 1.94E-27 YHR170W NMD3 het Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
0.182 2.58E-26 YGL246C RAI1 hom Nuclear protein with decapping endonuclease activity targeted toward mRNAs with unmethylated 7-methylguanosine cap structures; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; homologous to human DOM3Z
0.181 5.30E-26 YGL099W LSG1 het Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
0.179 1.68E-25 YLR061W RPL22A hom Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication
0.167 2.57E-22 YJL188C_d BUD19_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay
0.164 1.38E-21 YLR062C_d BUD28_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene RPL22A; diploid mutant displays a weak budding pattern phenotype in a systematic assay
0.159 2.47E-20 YDR140W MTQ2 hom S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC
0.154 2.29E-19 YHR205W SCH9 hom AGC family protein kinase; functional ortholog of mammalian S6 kinase; phosphorylated by Tor1p and required for TORC1-mediated regulation of ribosome biogenesis, translation initiation, and entry into G0 phase; involved in transactivation of osmostress-responsive genes; regulates G1 progression, cAPK activity and nitrogen activation of the FGM pathway; integrates nutrient signals and stress signals from sphingolipids to regulate lifespan
0.151 1.63E-18 YPL093W NOG1 het Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins
0.148 5.65E-18 YLR048W RPS0B hom Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2
0.147 1.16E-17 YFL033C RIM15 hom Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase
0.143 9.87E-17 YDR005C MAF1 hom Highly conserved negative regulator of RNA polymerase III; binds to the N-terminal domain of the Rpc160p subunit of Pol III to prevent closed-complex formation; localization and activity are regulated by phosphorylation, mediated by TORC1, protein kinase A, and Sch9p; localizes to cytoplasm during vegetative growth and translocates to the nucleus and nucleolus under stress conditions