YBR271W / EFM2

S-adenosylmethionine-dependent methyltransferase; methylates translation elongation factors EF2 (Eft1p and Eft2p) and EF3A (Yef3p); belongs to the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; predicted to be involved in ribosome biogenesis

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR271W / EFM2

Click on Significant Values for Screen Details ID:SGTC_9|Compound:0092-0002|FD-Score:3.47|P-value:4.72E-4 ID:SGTC_69|Compound:0096-0274|FD-Score:3.53|P-value:3.88E-4 ID:SGTC_330|Compound:1488-0319|FD-Score:3.78|P-value:1.52E-4 ID:SGTC_424|Compound:splitomicin|FD-Score:5.84|P-value:9.57E-9 ID:SGTC_470|Compound:aa-861|FD-Score:4.39|P-value:1.32E-5 ID:SGTC_578|Compound:3460-0051|FD-Score:3.44|P-value:5.17E-4 ID:SGTC_583|Compound:1488-1091|FD-Score:4.36|P-value:1.46E-5 ID:SGTC_795|Compound:0685-0265|FD-Score:3.32|P-value:7.79E-4 ID:SGTC_796|Compound:1234-0020|FD-Score:5.82|P-value:1.04E-8 ID:SGTC_855|Compound:0428-0020|FD-Score:3.40|P-value:6.00E-4 ID:SGTC_944|Compound:3370-0224|FD-Score:4.49|P-value:8.38E-6 ID:SGTC_1205|Compound:4161-2785|FD-Score:4.18|P-value:3.11E-5 ID:SGTC_1246|Compound:0422-0085|FD-Score:4.88|P-value:1.42E-6 ID:SGTC_1387|Compound:3342-1166|FD-Score:3.27|P-value:9.38E-4 ID:SGTC_1459|Compound:k035-0031|FD-Score:4.75|P-value:2.54E-6 ID:SGTC_1570|Compound:verbenone|FD-Score:-3.57|P-value:1.77E-4 ID:SGTC_1626|Compound:st003712|FD-Score:4.08|P-value:4.80E-5 ID:SGTC_1915|Compound:st067105|FD-Score:3.92|P-value:9.04E-5 ID:SGTC_2119|Compound:5528445|FD-Score:3.71|P-value:2.02E-4 ID:SGTC_2254|Compound:7938975|FD-Score:3.30|P-value:8.30E-4 ID:SGTC_2262|Compound:9014885|FD-Score:-3.49|P-value:2.32E-4 ID:SGTC_2341|Compound:7987486|FD-Score:-3.11|P-value:8.80E-4 ID:SGTC_2384|Compound:9036245|FD-Score:-3.71|P-value:1.02E-4 ID:SGTC_2471|Compound:5466956|FD-Score:3.37|P-value:6.56E-4 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:-5.02|P-value:3.02E-7 ID:SGTC_3076|Compound:9116187|FD-Score:-3.08|P-value:9.75E-4 ID:SGTC_3080|Compound:9117754|FD-Score:3.34|P-value:7.31E-4 ID:SGTC_3084|Compound:9116877|FD-Score:-4.41|P-value:5.55E-6 ID:SGTC_3086|Compound:9117350|FD-Score:-3.59|P-value:1.64E-4 ID:SGTC_9|Compound:0092-0002|FD-Score:3.47|P-value:4.72E-4 ID:SGTC_69|Compound:0096-0274|FD-Score:3.53|P-value:3.88E-4 ID:SGTC_330|Compound:1488-0319|FD-Score:3.78|P-value:1.52E-4 ID:SGTC_424|Compound:splitomicin|FD-Score:5.84|P-value:9.57E-9 ID:SGTC_470|Compound:aa-861|FD-Score:4.39|P-value:1.32E-5 ID:SGTC_578|Compound:3460-0051|FD-Score:3.44|P-value:5.17E-4 ID:SGTC_583|Compound:1488-1091|FD-Score:4.36|P-value:1.46E-5 ID:SGTC_795|Compound:0685-0265|FD-Score:3.32|P-value:7.79E-4 ID:SGTC_796|Compound:1234-0020|FD-Score:5.82|P-value:1.04E-8 ID:SGTC_855|Compound:0428-0020|FD-Score:3.40|P-value:6.00E-4 ID:SGTC_944|Compound:3370-0224|FD-Score:4.49|P-value:8.38E-6 ID:SGTC_1205|Compound:4161-2785|FD-Score:4.18|P-value:3.11E-5 ID:SGTC_1246|Compound:0422-0085|FD-Score:4.88|P-value:1.42E-6 ID:SGTC_1387|Compound:3342-1166|FD-Score:3.27|P-value:9.38E-4 ID:SGTC_1459|Compound:k035-0031|FD-Score:4.75|P-value:2.54E-6 ID:SGTC_1570|Compound:verbenone|FD-Score:-3.57|P-value:1.77E-4 ID:SGTC_1626|Compound:st003712|FD-Score:4.08|P-value:4.80E-5 ID:SGTC_1915|Compound:st067105|FD-Score:3.92|P-value:9.04E-5 ID:SGTC_2119|Compound:5528445|FD-Score:3.71|P-value:2.02E-4 ID:SGTC_2254|Compound:7938975|FD-Score:3.30|P-value:8.30E-4 ID:SGTC_2262|Compound:9014885|FD-Score:-3.49|P-value:2.32E-4 ID:SGTC_2341|Compound:7987486|FD-Score:-3.11|P-value:8.80E-4 ID:SGTC_2384|Compound:9036245|FD-Score:-3.71|P-value:1.02E-4 ID:SGTC_2471|Compound:5466956|FD-Score:3.37|P-value:6.56E-4 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:-5.02|P-value:3.02E-7 ID:SGTC_3076|Compound:9116187|FD-Score:-3.08|P-value:9.75E-4 ID:SGTC_3080|Compound:9117754|FD-Score:3.34|P-value:7.31E-4 ID:SGTC_3084|Compound:9116877|FD-Score:-4.41|P-value:5.55E-6 ID:SGTC_3086|Compound:9117350|FD-Score:-3.59|P-value:1.64E-4

Top fitness defect scores for YBR271W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_424 splitomicin 5.84 100.0 mM 9.57E-9 3 2.67E-9
2 SGTC_796 1234-0020 5.82 70.7 µM 1.04E-8 SWF1 & branched chain AA biosynthesis 6 2.91E-9
3 SGTC_1246 0422-0085 4.88 105.0 µM 1.42E-6 12 5.41E-7
4 SGTC_1459 k035-0031 4.75 35.7 µM 2.54E-6 DNA intercalators 11 1.00E-6
5 SGTC_944 3370-0224 4.49 231.0 µM 8.38E-6 16 3.55E-6
6 SGTC_470 aa-861 4.39 153.0 µM 1.32E-5 sphingolipid biosynthesis & PDR1 40 5.77E-6
7 SGTC_583 1488-1091 4.36 88.9 µM 1.46E-5 12 6.42E-6
8 SGTC_1205 4161-2785 4.18 76.0 µM 3.11E-5 20 1.43E-5
9 SGTC_1626 st003712 4.08 1.8 µM 4.80E-5 21 2.28E-5
10 SGTC_1915 st067105 3.92 30.6 µM 9.04E-5 RNA pol III & RNase P/MRP 44 4.47E-5
11 SGTC_330 1488-0319 3.78 80.1 µM 1.52E-4 RPP1 & pyrimidine depletion 13 7.78E-5
12 SGTC_2119 5528445 3.71 120.6 µM 2.02E-4 27 1.05E-4
13 SGTC_69 0096-0274 3.53 206.7 µM 3.88E-4 mitochondrial processes 62 2.11E-4
14 SGTC_9 0092-0002 3.47 10.4 µM 4.72E-4 51 2.60E-4
15 SGTC_578 3460-0051 3.44 92.0 µM 5.17E-4 25 2.87E-4
16 SGTC_855 0428-0020 3.40 25.0 µM 6.00E-4 Golgi 37 3.36E-4
17 SGTC_2471 5466956 3.37 24.8 µM 6.56E-4 mitochondrial processes 23 3.70E-4
18 SGTC_3080 9117754 3.34 49.5 µM 7.31E-4 38 4.15E-4
19 SGTC_795 0685-0265 3.32 78.0 µM 7.79E-4 excess fatty acid 48 4.44E-4
20 SGTC_2254 7938975 3.30 95.2 µM 8.30E-4 75 4.75E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.124 5.32E-13 YOR294W RRS1 het Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice
0.108 3.31E-10 YPL244C HUT1 hom Protein with a role in UDP-galactose transport to the Golgi lumen, has similarity to human UDP-galactose transporter UGTrel1, exhibits a genetic interaction with S. cerevisiae ERO1
0.105 1.16E-9 YPL243W SRP68 het Core component of the signal recognition particle (SRP) complex; SRP complex functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
0.101 4.99E-9 YDR225W HTA1 hom Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p
0.101 5.38E-9 YGR236C SPG1 hom Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.097 1.92E-8 YDL058W USO1 het Essential protein involved in the vesicle-mediated ER to Golgi transport step of secretion; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex
0.095 3.31E-8 YER057C HMF1 hom Member of the p14.5 protein family with similarity to Mmf1p, functionally complements Mmf1p function when targeted to mitochondria; heat shock inducible; high-dosage growth inhibitor; forms a homotrimer in vitro
0.095 3.66E-8 YMR208W ERG12 het Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
0.091 1.34E-7 YGL075C MPS2 het Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required for insertion of the newly duplicated SPB into the nuclear envelope; potentially phosphorylated by Cdc28p
0.089 2.56E-7 YMR006C PLB2 hom Phospholipase B (lysophospholipase) involved in phospholipid metabolism; displays transacylase activity in vitro; overproduction confers resistance to lysophosphatidylcholine
0.088 3.67E-7 YJR097W JJJ3 hom Protein of unknown function, contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4
0.087 4.30E-7 YDL045C FAD1 het Flavin adenine dinucleotide (FAD) synthetase, performs the second step in synthesis of FAD from riboflavin
0.086 5.34E-7 YOR278W HEM4 het Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria
0.083 1.58E-6 YIL009C-A EST3 hom Component of the telomerase holoenzyme, involved in telomere replication
0.082 1.73E-6 YML130C ERO1 het Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds