HIPHOP chemogenomics database

YBR279W / PAF1

Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1

Zygosity: Homozygous strain

fixedexpanded
Profile for YBR279W / PAF1

Click on Significant Values for Screen Details

Top fitness defect scores for YBR279W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_1039	k821-0043	6.91	94.0 µM	2.36E-10	Golgi	4	2.42E-12
2	SGTC_2408	sulbentine	6.07	18.4 µM	2.53E-8		23	6.32E-10
3	SGTC_1112	tnp00069	5.93	1.2 µM	5.36E-8		3	1.54E-9
4	SGTC_648	0375-0473	5.89	33.3 µM	6.52E-8		2	1.95E-9
5	SGTC_2546	rhodinyl acetate	5.84	100.0 µM	8.17E-8		4	2.55E-9
6	SGTC_2912	7964298	5.83	10.9 µM	8.96E-8	cell wall	49	2.85E-9
7	SGTC_1511	0906-3109	5.81	28.5 µM	9.86E-8	excess fatty acid	14	3.19E-9
8	SGTC_1021	4476-0760	5.63	85.3 µM	2.35E-7		5	9.00E-9
9	SGTC_1071	trifluoperazine	5.49	6.3 µM	4.63E-7		8	2.02E-8
10	SGTC_3114	9122766	5.42	49.5 µM	6.28E-7		5	2.92E-8
11	SGTC_2550	chrysin dimethyl ether	5.28	71.9 µM	1.23E-6		2	6.50E-8
12	SGTC_243	5hr heat shock (37°C) + splitomicin	5.08	1.0 µM	3.04E-6	heat shock/prefoldin	23	1.92E-7
13	SGTC_1073	triclabendazole	5.01	3.1 µM	4.05E-6		12	2.71E-7
14	SGTC_1892	st058411	4.77	7.4 µM	1.11E-5		10	9.04E-7
15	SGTC_47	0149-0135	4.75	24.2 µM	1.20E-5	TSC3-RPN4	10	9.95E-7
16	SGTC_2953	9074162	4.71	66.5 µM	1.44E-5		12	1.24E-6
17	SGTC_791	1348-1292	4.70	225.0 µM	1.48E-5		21	1.28E-6
18	SGTC_2332	7982454	4.69	138.9 µM	1.53E-5		32	1.34E-6
19	SGTC_1012	4112-3522	4.57	18.0 µM	2.51E-5		15	2.42E-6
20	SGTC_61	0978-8327	4.49	98.6 µM	3.40E-5	ergosterol biosynthesis	17	3.48E-6

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.105	9.34E-10	YGR049W	SCM4	hom	Mitochondrial outer membrane protein of unknown function; predicted to have 4 transmembrane segments; import is mediated by Tom70p and Mim1p; interacts genetically with a cdc4 mutation
0.094	5.41E-8	YLR027C	AAT2	hom	Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells
0.085	7.31E-7	YLR200W	YKE2	hom	Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin
0.085	8.36E-7	YDR069C	DOA4	hom	Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole
0.085	8.95E-7	YKL007W	CAP1	hom	Alpha subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress
0.079	4.42E-6	YDR260C	SWM1	hom	Subunit of the anaphase-promoting complex, which is an E3 ubiquitin ligase that regulates the metaphase-anaphase transition and exit from mitosis; required for activation of the daughter-specific gene expression and spore wall maturation
0.078	5.38E-6	YDR348C	PAL1	hom	Protein of unknown function thought to be involved in endocytosis; Pal1p physically interacts with Ede1p and is found at endocytic sites at the cell periphery during the early stages of endocytosis; green fluorescent protein (GFP)-fusion protein also localizes to the bud neck; potential Cdc28p substrate; similar to the S. pombe Pal1 protein; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.076	9.73E-6	YCR077C	PAT1	hom	Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress
0.072	2.98E-5	YLR048W	RPS0B	hom	Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2
0.071	4.34E-5	YJL011C	RPC17	het	RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress
0.070	4.37E-5	YJL095W	BCK1	hom	Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p
0.069	6.82E-5	YDL115C	IWR1	hom	RNA polymerase II transport factor, conserved from yeast to humans; also has a role in transporting RNA polymerase III into the nucleus; interacts with most of the RNAP II subunits; nucleo-cytoplasmic shuttling protein; deletion causes hypersensitivity to K1 killer toxin; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress
0.069	6.94E-5	YNR016C	ACC1	het	Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids;
0.067	9.91E-5	YDR293C	SSD1	hom	Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function
0.067	1.01E-4	YDR162C	NBP2	hom	Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM)