YBR281C / DUG2

Component of glutamine amidotransferase (GATase II); forms a complex with Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p)

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR281C / DUG2

Click on Significant Values for Screen Details ID:SGTC_21|Compound:0849-0113|FD-Score:3.87|P-value:4.78E-5 ID:SGTC_42|Compound:0133-0086|FD-Score:3.49|P-value:2.19E-4 ID:SGTC_55|Compound:0767-0285|FD-Score:3.23|P-value:5.53E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:7.64|P-value:8.83E-15 ID:SGTC_119|Compound:0121-0043|FD-Score:3.94|P-value:3.60E-5 ID:SGTC_178|Compound:4092-0806|FD-Score:3.25|P-value:5.24E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:4.60|P-value:1.87E-6 ID:SGTC_250|Compound:brefeldin a|FD-Score:3.45|P-value:2.51E-4 ID:SGTC_350|Compound:k064-0020|FD-Score:3.36|P-value:3.50E-4 ID:SGTC_359|Compound:0081-0058|FD-Score:5.22|P-value:7.45E-8 ID:SGTC_364|Compound:0083-0097|FD-Score:7.60|P-value:1.15E-14 ID:SGTC_421|Compound:streptovitacin|FD-Score:3.10|P-value:8.93E-4 ID:SGTC_422|Compound:cantharidin|FD-Score:5.86|P-value:1.91E-9 ID:SGTC_439|Compound:amiodarone|FD-Score:3.11|P-value:8.66E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.41|P-value:3.52E-4 ID:SGTC_495|Compound:decylubiquinone|FD-Score:3.10|P-value:8.84E-4 ID:SGTC_619|Compound:1652-1634|FD-Score:4.61|P-value:1.79E-6 ID:SGTC_643|Compound:k048-0106|FD-Score:3.34|P-value:3.72E-4 ID:SGTC_672|Compound:1441-0018|FD-Score:3.49|P-value:2.18E-4 ID:SGTC_738|Compound:1315-0003|FD-Score:6.41|P-value:6.02E-11 ID:SGTC_747|Compound:3448-8087|FD-Score:3.59|P-value:1.51E-4 ID:SGTC_768|Compound:0384-0033|FD-Score:3.32|P-value:4.14E-4 ID:SGTC_788|Compound:1182-0090|FD-Score:3.60|P-value:1.45E-4 ID:SGTC_867|Compound:0518-0118|FD-Score:6.41|P-value:5.83E-11 ID:SGTC_951|Compound:1171-0579|FD-Score:4.41|P-value:4.47E-6 ID:SGTC_1218|Compound:1348-1416|FD-Score:3.81|P-value:6.32E-5 ID:SGTC_1229|Compound:0139-0193|FD-Score:3.25|P-value:5.19E-4 ID:SGTC_1368|Compound:1852-0141|FD-Score:3.18|P-value:6.60E-4 ID:SGTC_1369|Compound:1866-0067|FD-Score:-3.33|P-value:4.67E-4 ID:SGTC_1511|Compound:0906-3109|FD-Score:3.69|P-value:1.00E-4 ID:SGTC_1567|Compound:st069303|FD-Score:4.14|P-value:1.51E-5 ID:SGTC_1576|Compound:erythromycin ethylsuccinate|FD-Score:3.10|P-value:8.66E-4 ID:SGTC_1677|Compound:st019204|FD-Score:5.58|P-value:1.03E-8 ID:SGTC_1681|Compound:tripelennamine|FD-Score:4.00|P-value:2.77E-5 ID:SGTC_1732|Compound:st034308|FD-Score:3.66|P-value:1.14E-4 ID:SGTC_1774|Compound:st048371|FD-Score:3.48|P-value:2.27E-4 ID:SGTC_1797|Compound:st048695|FD-Score:3.12|P-value:8.21E-4 ID:SGTC_1848|Compound:st056003|FD-Score:3.74|P-value:8.33E-5 ID:SGTC_1894|Compound:3',6'-dihydroxyflavone|FD-Score:3.30|P-value:4.42E-4 ID:SGTC_1906|Compound:st060206|FD-Score:3.35|P-value:3.64E-4 ID:SGTC_1974|Compound:st069448|FD-Score:3.18|P-value:6.69E-4 ID:SGTC_1982|Compound:st072467|FD-Score:3.76|P-value:7.62E-5 ID:SGTC_1989|Compound:st071868|FD-Score:3.74|P-value:8.31E-5 ID:SGTC_2000|Compound:st075254|FD-Score:3.81|P-value:6.23E-5 ID:SGTC_2021|Compound:4019055|FD-Score:3.15|P-value:7.47E-4 ID:SGTC_2059|Compound:5238311|FD-Score:3.08|P-value:9.42E-4 ID:SGTC_2106|Compound:5357045|FD-Score:3.38|P-value:3.32E-4 ID:SGTC_2179|Compound:6298986|FD-Score:4.12|P-value:1.67E-5 ID:SGTC_2352|Compound:9027995|FD-Score:3.82|P-value:5.93E-5 ID:SGTC_2357|Compound:9067248|FD-Score:3.76|P-value:7.72E-5 ID:SGTC_2403|Compound:6032726|FD-Score:3.64|P-value:1.24E-4 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:-3.26|P-value:6.13E-4 ID:SGTC_2616|Compound:abietic acid|FD-Score:-3.21|P-value:7.21E-4 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:3.59|P-value:1.47E-4 ID:SGTC_2747|Compound:doxorubicin|FD-Score:3.40|P-value:3.01E-4 ID:SGTC_2813|Compound:7971799|FD-Score:-3.44|P-value:3.15E-4 ID:SGTC_2854|Compound:9024559|FD-Score:3.49|P-value:2.18E-4 ID:SGTC_2921|Compound:7974240|FD-Score:3.85|P-value:5.36E-5 ID:SGTC_3006|Compound:9075424|FD-Score:3.46|P-value:2.43E-4 ID:SGTC_3011|Compound:9081274|FD-Score:3.73|P-value:8.54E-5 ID:SGTC_3060|Compound:1327-0027|FD-Score:4.72|P-value:1.01E-6 ID:SGTC_3066|Compound:9117245|FD-Score:4.72|P-value:1.04E-6 ID:SGTC_3127|Compound:9126946|FD-Score:3.10|P-value:8.87E-4 ID:SGTC_3201|Compound:9113999|FD-Score:3.74|P-value:8.15E-5 ID:SGTC_3211|Compound:9128781|FD-Score:-4.90|P-value:5.31E-7 ID:SGTC_3261|Compound:9137475|FD-Score:3.42|P-value:2.87E-4 ID:SGTC_3266|Compound:9136860|FD-Score:3.49|P-value:2.20E-4 ID:SGTC_3275|Compound:9138069|FD-Score:-3.40|P-value:3.69E-4 ID:SGTC_3292|Compound:9120825|FD-Score:3.10|P-value:8.73E-4 ID:SGTC_21|Compound:0849-0113|FD-Score:3.87|P-value:4.78E-5 ID:SGTC_42|Compound:0133-0086|FD-Score:3.49|P-value:2.19E-4 ID:SGTC_55|Compound:0767-0285|FD-Score:3.23|P-value:5.53E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:7.64|P-value:8.83E-15 ID:SGTC_119|Compound:0121-0043|FD-Score:3.94|P-value:3.60E-5 ID:SGTC_178|Compound:4092-0806|FD-Score:3.25|P-value:5.24E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:4.60|P-value:1.87E-6 ID:SGTC_250|Compound:brefeldin a|FD-Score:3.45|P-value:2.51E-4 ID:SGTC_350|Compound:k064-0020|FD-Score:3.36|P-value:3.50E-4 ID:SGTC_359|Compound:0081-0058|FD-Score:5.22|P-value:7.45E-8 ID:SGTC_364|Compound:0083-0097|FD-Score:7.60|P-value:1.15E-14 ID:SGTC_421|Compound:streptovitacin|FD-Score:3.10|P-value:8.93E-4 ID:SGTC_422|Compound:cantharidin|FD-Score:5.86|P-value:1.91E-9 ID:SGTC_439|Compound:amiodarone|FD-Score:3.11|P-value:8.66E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.41|P-value:3.52E-4 ID:SGTC_495|Compound:decylubiquinone|FD-Score:3.10|P-value:8.84E-4 ID:SGTC_619|Compound:1652-1634|FD-Score:4.61|P-value:1.79E-6 ID:SGTC_643|Compound:k048-0106|FD-Score:3.34|P-value:3.72E-4 ID:SGTC_672|Compound:1441-0018|FD-Score:3.49|P-value:2.18E-4 ID:SGTC_738|Compound:1315-0003|FD-Score:6.41|P-value:6.02E-11 ID:SGTC_747|Compound:3448-8087|FD-Score:3.59|P-value:1.51E-4 ID:SGTC_768|Compound:0384-0033|FD-Score:3.32|P-value:4.14E-4 ID:SGTC_788|Compound:1182-0090|FD-Score:3.60|P-value:1.45E-4 ID:SGTC_867|Compound:0518-0118|FD-Score:6.41|P-value:5.83E-11 ID:SGTC_951|Compound:1171-0579|FD-Score:4.41|P-value:4.47E-6 ID:SGTC_1218|Compound:1348-1416|FD-Score:3.81|P-value:6.32E-5 ID:SGTC_1229|Compound:0139-0193|FD-Score:3.25|P-value:5.19E-4 ID:SGTC_1368|Compound:1852-0141|FD-Score:3.18|P-value:6.60E-4 ID:SGTC_1369|Compound:1866-0067|FD-Score:-3.33|P-value:4.67E-4 ID:SGTC_1511|Compound:0906-3109|FD-Score:3.69|P-value:1.00E-4 ID:SGTC_1567|Compound:st069303|FD-Score:4.14|P-value:1.51E-5 ID:SGTC_1576|Compound:erythromycin ethylsuccinate|FD-Score:3.10|P-value:8.66E-4 ID:SGTC_1677|Compound:st019204|FD-Score:5.58|P-value:1.03E-8 ID:SGTC_1681|Compound:tripelennamine|FD-Score:4.00|P-value:2.77E-5 ID:SGTC_1732|Compound:st034308|FD-Score:3.66|P-value:1.14E-4 ID:SGTC_1774|Compound:st048371|FD-Score:3.48|P-value:2.27E-4 ID:SGTC_1797|Compound:st048695|FD-Score:3.12|P-value:8.21E-4 ID:SGTC_1848|Compound:st056003|FD-Score:3.74|P-value:8.33E-5 ID:SGTC_1894|Compound:3',6'-dihydroxyflavone|FD-Score:3.30|P-value:4.42E-4 ID:SGTC_1906|Compound:st060206|FD-Score:3.35|P-value:3.64E-4 ID:SGTC_1974|Compound:st069448|FD-Score:3.18|P-value:6.69E-4 ID:SGTC_1982|Compound:st072467|FD-Score:3.76|P-value:7.62E-5 ID:SGTC_1989|Compound:st071868|FD-Score:3.74|P-value:8.31E-5 ID:SGTC_2000|Compound:st075254|FD-Score:3.81|P-value:6.23E-5 ID:SGTC_2021|Compound:4019055|FD-Score:3.15|P-value:7.47E-4 ID:SGTC_2059|Compound:5238311|FD-Score:3.08|P-value:9.42E-4 ID:SGTC_2106|Compound:5357045|FD-Score:3.38|P-value:3.32E-4 ID:SGTC_2179|Compound:6298986|FD-Score:4.12|P-value:1.67E-5 ID:SGTC_2352|Compound:9027995|FD-Score:3.82|P-value:5.93E-5 ID:SGTC_2357|Compound:9067248|FD-Score:3.76|P-value:7.72E-5 ID:SGTC_2403|Compound:6032726|FD-Score:3.64|P-value:1.24E-4 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:-3.26|P-value:6.13E-4 ID:SGTC_2616|Compound:abietic acid|FD-Score:-3.21|P-value:7.21E-4 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:3.59|P-value:1.47E-4 ID:SGTC_2747|Compound:doxorubicin|FD-Score:3.40|P-value:3.01E-4 ID:SGTC_2813|Compound:7971799|FD-Score:-3.44|P-value:3.15E-4 ID:SGTC_2854|Compound:9024559|FD-Score:3.49|P-value:2.18E-4 ID:SGTC_2921|Compound:7974240|FD-Score:3.85|P-value:5.36E-5 ID:SGTC_3006|Compound:9075424|FD-Score:3.46|P-value:2.43E-4 ID:SGTC_3011|Compound:9081274|FD-Score:3.73|P-value:8.54E-5 ID:SGTC_3060|Compound:1327-0027|FD-Score:4.72|P-value:1.01E-6 ID:SGTC_3066|Compound:9117245|FD-Score:4.72|P-value:1.04E-6 ID:SGTC_3127|Compound:9126946|FD-Score:3.10|P-value:8.87E-4 ID:SGTC_3201|Compound:9113999|FD-Score:3.74|P-value:8.15E-5 ID:SGTC_3211|Compound:9128781|FD-Score:-4.90|P-value:5.31E-7 ID:SGTC_3261|Compound:9137475|FD-Score:3.42|P-value:2.87E-4 ID:SGTC_3266|Compound:9136860|FD-Score:3.49|P-value:2.20E-4 ID:SGTC_3275|Compound:9138069|FD-Score:-3.40|P-value:3.69E-4 ID:SGTC_3292|Compound:9120825|FD-Score:3.10|P-value:8.73E-4

Top fitness defect scores for YBR281C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_58 0957-0393 7.64 9.0 µM 8.83E-15 cell wall 138 1.12E-14
2 SGTC_364 0083-0097 7.60 67.7 µM 1.15E-14 2 1.46E-14
3 SGTC_867 0518-0118 6.41 155.0 µM 5.83E-11 DNA intercalators 3 7.10E-11
4 SGTC_738 1315-0003 6.41 72.4 µM 6.02E-11 2 7.33E-11
5 SGTC_422 cantharidin 5.86 155.0 µM 1.91E-9 cell wall signaling 21 2.28E-9
6 SGTC_1677 st019204 5.58 53.9 µM 1.03E-8 3 1.22E-8
7 SGTC_359 0081-0058 5.22 15.9 µM 7.45E-8 60S ribosome export 16 8.72E-8
8 SGTC_3060 1327-0027 4.72 12.5 µM 1.01E-6 26 1.17E-6
9 SGTC_3066 9117245 4.72 49.5 µM 1.04E-6 6 1.20E-6
10 SGTC_619 1652-1634 4.61 108.0 µM 1.79E-6 PDR1 12 2.06E-6
11 SGTC_221 trifluoperazine 4.60 10.3 µM 1.87E-6 NEO1-PIK1 182 2.15E-6
12 SGTC_951 1171-0579 4.41 82.7 µM 4.47E-6 cell wall 58 5.10E-6
13 SGTC_1567 st069303 4.14 68.4 µM 1.51E-5 mitochondrial processes 18 1.71E-5
14 SGTC_2179 6298986 4.12 200.0 µM 1.67E-5 heme requiring 19 1.89E-5
15 SGTC_1681 tripelennamine 4.00 100.0 µM 2.77E-5 20 3.12E-5
16 SGTC_119 0121-0043 3.94 24.8 µM 3.60E-5 mitochondrial stress 112 4.05E-5
17 SGTC_21 0849-0113 3.87 23.0 µM 4.78E-5 35 5.36E-5
18 SGTC_2921 7974240 3.85 10.7 µM 5.36E-5 61 6.02E-5
19 SGTC_2352 9027995 3.82 87.8 µM 5.93E-5 tubulin folding & SWR complex 34 6.65E-5
20 SGTC_2000 st075254 3.81 36.4 µM 6.23E-5 8 6.98E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.162 2.87E-21 YBR296C PHO89 hom Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification
0.146 2.18E-17 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.142 1.24E-16 YBR120C CBP6 hom Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.141 1.78E-16 YOR231W MKK1 hom MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication
0.138 9.37E-16 YBR286W APE3 hom Vacuolar aminopeptidase Y, processed to mature form by Prb1p
0.135 4.30E-15 YBR295W PCA1 hom Cadmium transporting P-type ATPase; may also have a role in copper and iron homeostasis; stabilized by Cd binding, which prevents ubiquitination; S288C and other lab strains contain a G970R mutation which eliminates Cd transport function
0.134 5.66E-15 YCL005W LDB16 hom Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria
0.132 1.72E-14 YDL025C RTK1 hom Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress
0.132 1.88E-14 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.130 4.59E-14 YNL314W DAL82 hom Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain
0.130 4.64E-14 YKL033W-A_p YKL033W-A_p hom Putative protein of unknown function; similar to uncharacterized proteins from other fungi
0.129 5.41E-14 YLL040C VPS13 hom Protein of unknown function; heterooligomeric or homooligomeric complex; peripherally associated with membranes; involved in sporulation, vacuolar protein sorting, prospore membrane formation and protein-Golgi retention; homologous to human CHAC and COH1 which are involved in chorea acanthocytosis and Cohen syndrome, respectively
0.123 7.66E-13 YCR087C-A_p YCR087C-A_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene
0.122 1.07E-12 YIL148W RPL40A hom Ubiquitin-ribosomal 60S subunit protein L40A fusion protein; cleaved to yield ubiquitin and ribosomal protein L40A; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40A has a paralog, RPL40B, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
0.122 1.09E-12 YNL115C_p YNL115C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL115C is not an essential gene