YBR296C / PHO89

Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR296C / PHO89

Click on Significant Values for Screen Details ID:SGTC_18|Compound:0111-0177|FD-Score:3.98|P-value:2.80E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:11.00|P-value:2.00E-21 ID:SGTC_146|Compound:3448-8650|FD-Score:3.65|P-value:7.65E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:6.47|P-value:1.21E-8 ID:SGTC_223|Compound:busulfan|FD-Score:3.80|P-value:4.88E-4 ID:SGTC_250|Compound:brefeldin a|FD-Score:4.78|P-value:1.80E-5 ID:SGTC_284|Compound:0988-0104|FD-Score:-4.84|P-value:2.37E-5 ID:SGTC_287|Compound:k060-0039|FD-Score:4.94|P-value:9.57E-6 ID:SGTC_295|Compound:k015-0027|FD-Score:5.37|P-value:1.73E-6 ID:SGTC_305|Compound:1682-2595|FD-Score:-4.21|P-value:2.04E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.75|P-value:8.49E-4 ID:SGTC_345|Compound:1362-0200|FD-Score:-3.92|P-value:5.09E-4 ID:SGTC_355|Compound:1311-2252|FD-Score:-4.91|P-value:1.79E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-4.68|P-value:4.20E-5 ID:SGTC_568|Compound:1193-0097|FD-Score:4.62|P-value:3.24E-5 ID:SGTC_639|Compound:0833-0042|FD-Score:-4.00|P-value:3.96E-4 ID:SGTC_716|Compound:1683-5115|FD-Score:3.58|P-value:9.34E-4 ID:SGTC_768|Compound:0384-0033|FD-Score:6.33|P-value:2.42E-8 ID:SGTC_1036|Compound:k216-0215|FD-Score:4.21|P-value:1.34E-4 ID:SGTC_1096|Compound:rimcazole|FD-Score:3.95|P-value:3.03E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:3.58|P-value:9.17E-4 ID:SGTC_1361|Compound:1608-0041|FD-Score:4.30|P-value:9.89E-5 ID:SGTC_1367|Compound:1812-0510|FD-Score:5.83|P-value:2.48E-7 ID:SGTC_1479|Compound:0549-0158|FD-Score:3.86|P-value:4.07E-4 ID:SGTC_1492|Compound:4158-0558|FD-Score:3.74|P-value:5.86E-4 ID:SGTC_1681|Compound:tripelennamine|FD-Score:4.37|P-value:7.58E-5 ID:SGTC_1746|Compound:st038117|FD-Score:4.73|P-value:2.14E-5 ID:SGTC_1750|Compound:st081859|FD-Score:5.70|P-value:4.35E-7 ID:SGTC_1800|Compound:st050079|FD-Score:4.46|P-value:5.66E-5 ID:SGTC_1957|Compound:st077035|FD-Score:-4.36|P-value:1.27E-4 ID:SGTC_1974|Compound:st069448|FD-Score:5.17|P-value:4.02E-6 ID:SGTC_2021|Compound:4019055|FD-Score:3.96|P-value:2.94E-4 ID:SGTC_2161|Compound:5662504|FD-Score:4.90|P-value:1.12E-5 ID:SGTC_2286|Compound:7945697|FD-Score:-4.56|P-value:6.39E-5 ID:SGTC_2288|Compound:7946289|FD-Score:5.65|P-value:5.33E-7 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:3.58|P-value:9.21E-4 ID:SGTC_2432|Compound:5630864|FD-Score:3.73|P-value:6.04E-4 ID:SGTC_2505|Compound:galangin|FD-Score:3.76|P-value:5.47E-4 ID:SGTC_2543|Compound:pimpinellin|FD-Score:3.64|P-value:7.78E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:-5.78|P-value:5.51E-7 ID:SGTC_2704|Compound:st078022|FD-Score:6.03|P-value:1.00E-7 ID:SGTC_2782|Compound:7736369|FD-Score:3.64|P-value:7.76E-4 ID:SGTC_2802|Compound:7925815|FD-Score:-4.39|P-value:1.15E-4 ID:SGTC_2820|Compound:7989630|FD-Score:4.03|P-value:2.35E-4 ID:SGTC_3107|Compound:9121808|FD-Score:4.17|P-value:1.48E-4 ID:SGTC_3109|Compound:9122853|FD-Score:-5.13|P-value:7.91E-6 ID:SGTC_3115|Compound:9123671|FD-Score:-3.70|P-value:9.75E-4 ID:SGTC_3208|Compound:9114115|FD-Score:3.89|P-value:3.63E-4 ID:SGTC_18|Compound:0111-0177|FD-Score:3.98|P-value:2.80E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:11.00|P-value:2.00E-21 ID:SGTC_146|Compound:3448-8650|FD-Score:3.65|P-value:7.65E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:6.47|P-value:1.21E-8 ID:SGTC_223|Compound:busulfan|FD-Score:3.80|P-value:4.88E-4 ID:SGTC_250|Compound:brefeldin a|FD-Score:4.78|P-value:1.80E-5 ID:SGTC_284|Compound:0988-0104|FD-Score:-4.84|P-value:2.37E-5 ID:SGTC_287|Compound:k060-0039|FD-Score:4.94|P-value:9.57E-6 ID:SGTC_295|Compound:k015-0027|FD-Score:5.37|P-value:1.73E-6 ID:SGTC_305|Compound:1682-2595|FD-Score:-4.21|P-value:2.04E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.75|P-value:8.49E-4 ID:SGTC_345|Compound:1362-0200|FD-Score:-3.92|P-value:5.09E-4 ID:SGTC_355|Compound:1311-2252|FD-Score:-4.91|P-value:1.79E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-4.68|P-value:4.20E-5 ID:SGTC_568|Compound:1193-0097|FD-Score:4.62|P-value:3.24E-5 ID:SGTC_639|Compound:0833-0042|FD-Score:-4.00|P-value:3.96E-4 ID:SGTC_716|Compound:1683-5115|FD-Score:3.58|P-value:9.34E-4 ID:SGTC_768|Compound:0384-0033|FD-Score:6.33|P-value:2.42E-8 ID:SGTC_1036|Compound:k216-0215|FD-Score:4.21|P-value:1.34E-4 ID:SGTC_1096|Compound:rimcazole|FD-Score:3.95|P-value:3.03E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:3.58|P-value:9.17E-4 ID:SGTC_1361|Compound:1608-0041|FD-Score:4.30|P-value:9.89E-5 ID:SGTC_1367|Compound:1812-0510|FD-Score:5.83|P-value:2.48E-7 ID:SGTC_1479|Compound:0549-0158|FD-Score:3.86|P-value:4.07E-4 ID:SGTC_1492|Compound:4158-0558|FD-Score:3.74|P-value:5.86E-4 ID:SGTC_1681|Compound:tripelennamine|FD-Score:4.37|P-value:7.58E-5 ID:SGTC_1746|Compound:st038117|FD-Score:4.73|P-value:2.14E-5 ID:SGTC_1750|Compound:st081859|FD-Score:5.70|P-value:4.35E-7 ID:SGTC_1800|Compound:st050079|FD-Score:4.46|P-value:5.66E-5 ID:SGTC_1957|Compound:st077035|FD-Score:-4.36|P-value:1.27E-4 ID:SGTC_1974|Compound:st069448|FD-Score:5.17|P-value:4.02E-6 ID:SGTC_2021|Compound:4019055|FD-Score:3.96|P-value:2.94E-4 ID:SGTC_2161|Compound:5662504|FD-Score:4.90|P-value:1.12E-5 ID:SGTC_2286|Compound:7945697|FD-Score:-4.56|P-value:6.39E-5 ID:SGTC_2288|Compound:7946289|FD-Score:5.65|P-value:5.33E-7 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:3.58|P-value:9.21E-4 ID:SGTC_2432|Compound:5630864|FD-Score:3.73|P-value:6.04E-4 ID:SGTC_2505|Compound:galangin|FD-Score:3.76|P-value:5.47E-4 ID:SGTC_2543|Compound:pimpinellin|FD-Score:3.64|P-value:7.78E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:-5.78|P-value:5.51E-7 ID:SGTC_2704|Compound:st078022|FD-Score:6.03|P-value:1.00E-7 ID:SGTC_2782|Compound:7736369|FD-Score:3.64|P-value:7.76E-4 ID:SGTC_2802|Compound:7925815|FD-Score:-4.39|P-value:1.15E-4 ID:SGTC_2820|Compound:7989630|FD-Score:4.03|P-value:2.35E-4 ID:SGTC_3107|Compound:9121808|FD-Score:4.17|P-value:1.48E-4 ID:SGTC_3109|Compound:9122853|FD-Score:-5.13|P-value:7.91E-6 ID:SGTC_3115|Compound:9123671|FD-Score:-3.70|P-value:9.75E-4 ID:SGTC_3208|Compound:9114115|FD-Score:3.89|P-value:3.63E-4

Top fitness defect scores for YBR296C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_58 0957-0393 11.00 9.0 µM 2.00E-21 cell wall 66 2.13E-28
2 SGTC_221 trifluoperazine 6.47 10.3 µM 1.21E-8 NEO1-PIK1 110 4.83E-11
3 SGTC_768 0384-0033 6.33 77.3 µM 2.42E-8 fatty acid desaturase (OLE1) 34 1.23E-10
4 SGTC_2704 st078022 6.03 71.1 µM 1.00E-7 2 8.34E-10
5 SGTC_1367 1812-0510 5.83 76.9 µM 2.48E-7 2 2.83E-9
6 SGTC_1750 st081859 5.70 2.4 µM 4.35E-7 endomembrane recycling 114 6.03E-9
7 SGTC_2288 7946289 5.65 167.3 µM 5.33E-7 6 7.95E-9
8 SGTC_295 k015-0027 5.37 31.0 µM 1.73E-6 PDR1 4 3.87E-8
9 SGTC_1974 st069448 5.17 26.9 µM 4.02E-6 6 1.20E-7
10 SGTC_287 k060-0039 4.94 29.6 µM 9.57E-6 3 3.84E-7
11 SGTC_2161 5662504 4.90 8.5 µM 1.12E-5 tubulin folding & SWR complex 8 4.76E-7
12 SGTC_250 brefeldin a 4.78 552.2 µM 1.80E-5 PDR1 7 8.98E-7
13 SGTC_1746 st038117 4.73 42.0 µM 2.14E-5 18 1.13E-6
14 SGTC_568 1193-0097 4.62 7.5 µM 3.24E-5 ERAD & cell cycle 107 1.96E-6
15 SGTC_1800 st050079 4.46 29.1 µM 5.66E-5 NEO1-PIK1 98 4.14E-6
16 SGTC_1681 tripelennamine 4.37 100.0 µM 7.58E-5 13 6.12E-6
17 SGTC_1361 1608-0041 4.30 5.6 µM 9.89E-5 RPP1 & pyrimidine depletion 13 8.73E-6
18 SGTC_1036 k216-0215 4.21 33.5 µM 1.34E-4 mitochondrial response to ROS 43 1.30E-5
19 SGTC_3107 9121808 4.17 49.5 µM 1.48E-4 5 1.49E-5
20 SGTC_2820 7989630 4.03 71.4 µM 2.35E-4 ERG2 19 2.77E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.225 7.94E-40 YDL025C RTK1 hom Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress
0.208 4.76E-34 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.197 1.23E-30 YNL115C_p YNL115C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL115C is not an essential gene
0.191 4.83E-29 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.183 1.00E-26 YNL077W APJ1 hom Chaperone with a role in SUMO-mediated protein degradation; member of the DnaJ-like family; conserved across eukaryotes; overexpression interferes with propagation of the [Psi+] prion; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress
0.179 1.21E-25 YBR286W APE3 hom Vacuolar aminopeptidase Y, processed to mature form by Prb1p
0.178 2.94E-25 YBR120C CBP6 hom Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.175 1.98E-24 YGR058W PEF1 hom Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly
0.174 2.82E-24 YNL211C_p YNL211C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL211C is not an essential gene
0.172 1.16E-23 YLR150W STM1 hom Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery
0.168 1.49E-22 YMR126C_p DLT1_p hom Protein of unknown function, mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA)
0.163 1.97E-21 YOR231W MKK1 hom MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication
0.162 2.87E-21 YBR281C DUG2 hom Component of glutamine amidotransferase (GATase II); forms a complex with Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p)
0.161 5.03E-21 YNL314W DAL82 hom Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain
0.161 8.21E-21 YPR153W YPR153W hom Putative protein of unknown function