YCL005W / LDB16

Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria

Zygosity: Homozygous strain
fixedexpanded
Profile for YCL005W / LDB16

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:20.50|P-value:1.80E-81 ID:SGTC_138|Compound:0850-0259|FD-Score:5.66|P-value:8.81E-8 ID:SGTC_218|Compound:alverine citrate|FD-Score:3.58|P-value:5.03E-4 ID:SGTC_266|Compound:formosanin C|FD-Score:3.79|P-value:2.49E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:3.62|P-value:4.48E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:6.50|P-value:9.56E-10 ID:SGTC_309|Compound:k408-0051|FD-Score:4.38|P-value:2.72E-5 ID:SGTC_354|Compound:2783-3500|FD-Score:4.47|P-value:1.94E-5 ID:SGTC_413|Compound:propylparaben|FD-Score:4.33|P-value:3.32E-5 ID:SGTC_514|Compound:4466-0038|FD-Score:6.23|P-value:4.39E-9 ID:SGTC_528|Compound:3492-0019|FD-Score:9.54|P-value:4.99E-19 ID:SGTC_582|Compound:0987-0078|FD-Score:4.13|P-value:7.21E-5 ID:SGTC_863|Compound:0466-0191|FD-Score:4.79|P-value:5.16E-6 ID:SGTC_922|Compound:1866-0035|FD-Score:3.88|P-value:1.79E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:7.15|P-value:1.89E-11 ID:SGTC_971|Compound:1315-0384|FD-Score:4.20|P-value:5.54E-5 ID:SGTC_981|Compound:1488-1094|FD-Score:3.38|P-value:9.75E-4 ID:SGTC_1087|Compound:pterostilbene|FD-Score:3.57|P-value:5.17E-4 ID:SGTC_1175|Compound:0741-0056|FD-Score:3.74|P-value:2.96E-4 ID:SGTC_1255|Compound:0566-0045|FD-Score:4.51|P-value:1.65E-5 ID:SGTC_1294|Compound:1000-1494|FD-Score:4.01|P-value:1.12E-4 ID:SGTC_1393|Compound:3470-5649|FD-Score:4.24|P-value:4.73E-5 ID:SGTC_1472|Compound:k841-0093|FD-Score:3.45|P-value:7.82E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:4.28|P-value:4.03E-5 ID:SGTC_1679|Compound:4130-1276|FD-Score:4.14|P-value:6.99E-5 ID:SGTC_1712|Compound:st032288|FD-Score:4.07|P-value:9.23E-5 ID:SGTC_1733|Compound:st037357|FD-Score:3.90|P-value:1.70E-4 ID:SGTC_1734|Compound:st037168|FD-Score:3.46|P-value:7.48E-4 ID:SGTC_1736|Compound:st036774|FD-Score:5.85|P-value:3.36E-8 ID:SGTC_1847|Compound:st056231|FD-Score:5.06|P-value:1.53E-6 ID:SGTC_1905|Compound:st060207|FD-Score:4.14|P-value:7.03E-5 ID:SGTC_1957|Compound:st077035|FD-Score:3.77|P-value:2.63E-4 ID:SGTC_2082|Compound:5219693|FD-Score:3.97|P-value:1.29E-4 ID:SGTC_2270|Compound:7924575|FD-Score:3.96|P-value:1.36E-4 ID:SGTC_2358|Compound:9037157|FD-Score:-3.33|P-value:8.38E-4 ID:SGTC_2367|Compound:9070478|FD-Score:3.78|P-value:2.53E-4 ID:SGTC_2379|Compound:9035213|FD-Score:4.44|P-value:2.15E-5 ID:SGTC_2393|Compound:9031399|FD-Score:3.57|P-value:5.31E-4 ID:SGTC_2425|Compound:5628681|FD-Score:4.11|P-value:7.77E-5 ID:SGTC_2500|Compound:5-alpha-androstan-3,17-dione|FD-Score:4.01|P-value:1.13E-4 ID:SGTC_2542|Compound:2-hydroxy-5 (6)epoxy-tetrahydrocaryophyllene|FD-Score:4.21|P-value:5.31E-5 ID:SGTC_2584|Compound:androsterone|FD-Score:13.30|P-value:3.88E-35 ID:SGTC_2756|Compound:carbadox|FD-Score:3.44|P-value:7.89E-4 ID:SGTC_2815|Compound:7968870|FD-Score:3.48|P-value:7.08E-4 ID:SGTC_2821|Compound:7996764|FD-Score:-3.43|P-value:5.97E-4 ID:SGTC_2828|Compound:7998861|FD-Score:4.09|P-value:8.45E-5 ID:SGTC_2836|Compound:9010338|FD-Score:5.23|P-value:7.12E-7 ID:SGTC_2885|Compound:9057732|FD-Score:-4.83|P-value:2.83E-6 ID:SGTC_2948|Compound:9070189|FD-Score:-3.71|P-value:2.36E-4 ID:SGTC_2958|Compound:9077254|FD-Score:-3.42|P-value:6.21E-4 ID:SGTC_3082|Compound:9117008|FD-Score:6.46|P-value:1.20E-9 ID:SGTC_3194|Compound:9111903|FD-Score:-3.96|P-value:9.49E-5 ID:SGTC_3198|Compound:9112019|FD-Score:3.37|P-value:9.93E-4 ID:SGTC_3210|Compound:9128157|FD-Score:-4.09|P-value:5.90E-5 ID:SGTC_3266|Compound:9136860|FD-Score:5.74|P-value:5.83E-8 ID:SGTC_3285|Compound:9112818|FD-Score:5.38|P-value:3.49E-7 ID:SGTC_3311|Compound:9131146|FD-Score:7.51|P-value:1.89E-12 ID:SGTC_3321|Compound:9137793|FD-Score:3.52|P-value:6.22E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:20.50|P-value:1.80E-81 ID:SGTC_138|Compound:0850-0259|FD-Score:5.66|P-value:8.81E-8 ID:SGTC_218|Compound:alverine citrate|FD-Score:3.58|P-value:5.03E-4 ID:SGTC_266|Compound:formosanin C|FD-Score:3.79|P-value:2.49E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:3.62|P-value:4.48E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:6.50|P-value:9.56E-10 ID:SGTC_309|Compound:k408-0051|FD-Score:4.38|P-value:2.72E-5 ID:SGTC_354|Compound:2783-3500|FD-Score:4.47|P-value:1.94E-5 ID:SGTC_413|Compound:propylparaben|FD-Score:4.33|P-value:3.32E-5 ID:SGTC_514|Compound:4466-0038|FD-Score:6.23|P-value:4.39E-9 ID:SGTC_528|Compound:3492-0019|FD-Score:9.54|P-value:4.99E-19 ID:SGTC_582|Compound:0987-0078|FD-Score:4.13|P-value:7.21E-5 ID:SGTC_863|Compound:0466-0191|FD-Score:4.79|P-value:5.16E-6 ID:SGTC_922|Compound:1866-0035|FD-Score:3.88|P-value:1.79E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:7.15|P-value:1.89E-11 ID:SGTC_971|Compound:1315-0384|FD-Score:4.20|P-value:5.54E-5 ID:SGTC_981|Compound:1488-1094|FD-Score:3.38|P-value:9.75E-4 ID:SGTC_1087|Compound:pterostilbene|FD-Score:3.57|P-value:5.17E-4 ID:SGTC_1175|Compound:0741-0056|FD-Score:3.74|P-value:2.96E-4 ID:SGTC_1255|Compound:0566-0045|FD-Score:4.51|P-value:1.65E-5 ID:SGTC_1294|Compound:1000-1494|FD-Score:4.01|P-value:1.12E-4 ID:SGTC_1393|Compound:3470-5649|FD-Score:4.24|P-value:4.73E-5 ID:SGTC_1472|Compound:k841-0093|FD-Score:3.45|P-value:7.82E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:4.28|P-value:4.03E-5 ID:SGTC_1679|Compound:4130-1276|FD-Score:4.14|P-value:6.99E-5 ID:SGTC_1712|Compound:st032288|FD-Score:4.07|P-value:9.23E-5 ID:SGTC_1733|Compound:st037357|FD-Score:3.90|P-value:1.70E-4 ID:SGTC_1734|Compound:st037168|FD-Score:3.46|P-value:7.48E-4 ID:SGTC_1736|Compound:st036774|FD-Score:5.85|P-value:3.36E-8 ID:SGTC_1847|Compound:st056231|FD-Score:5.06|P-value:1.53E-6 ID:SGTC_1905|Compound:st060207|FD-Score:4.14|P-value:7.03E-5 ID:SGTC_1957|Compound:st077035|FD-Score:3.77|P-value:2.63E-4 ID:SGTC_2082|Compound:5219693|FD-Score:3.97|P-value:1.29E-4 ID:SGTC_2270|Compound:7924575|FD-Score:3.96|P-value:1.36E-4 ID:SGTC_2358|Compound:9037157|FD-Score:-3.33|P-value:8.38E-4 ID:SGTC_2367|Compound:9070478|FD-Score:3.78|P-value:2.53E-4 ID:SGTC_2379|Compound:9035213|FD-Score:4.44|P-value:2.15E-5 ID:SGTC_2393|Compound:9031399|FD-Score:3.57|P-value:5.31E-4 ID:SGTC_2425|Compound:5628681|FD-Score:4.11|P-value:7.77E-5 ID:SGTC_2500|Compound:5-alpha-androstan-3,17-dione|FD-Score:4.01|P-value:1.13E-4 ID:SGTC_2542|Compound:2-hydroxy-5 (6)epoxy-tetrahydrocaryophyllene|FD-Score:4.21|P-value:5.31E-5 ID:SGTC_2584|Compound:androsterone|FD-Score:13.30|P-value:3.88E-35 ID:SGTC_2756|Compound:carbadox|FD-Score:3.44|P-value:7.89E-4 ID:SGTC_2815|Compound:7968870|FD-Score:3.48|P-value:7.08E-4 ID:SGTC_2821|Compound:7996764|FD-Score:-3.43|P-value:5.97E-4 ID:SGTC_2828|Compound:7998861|FD-Score:4.09|P-value:8.45E-5 ID:SGTC_2836|Compound:9010338|FD-Score:5.23|P-value:7.12E-7 ID:SGTC_2885|Compound:9057732|FD-Score:-4.83|P-value:2.83E-6 ID:SGTC_2948|Compound:9070189|FD-Score:-3.71|P-value:2.36E-4 ID:SGTC_2958|Compound:9077254|FD-Score:-3.42|P-value:6.21E-4 ID:SGTC_3082|Compound:9117008|FD-Score:6.46|P-value:1.20E-9 ID:SGTC_3194|Compound:9111903|FD-Score:-3.96|P-value:9.49E-5 ID:SGTC_3198|Compound:9112019|FD-Score:3.37|P-value:9.93E-4 ID:SGTC_3210|Compound:9128157|FD-Score:-4.09|P-value:5.90E-5 ID:SGTC_3266|Compound:9136860|FD-Score:5.74|P-value:5.83E-8 ID:SGTC_3285|Compound:9112818|FD-Score:5.38|P-value:3.49E-7 ID:SGTC_3311|Compound:9131146|FD-Score:7.51|P-value:1.89E-12 ID:SGTC_3321|Compound:9137793|FD-Score:3.52|P-value:6.22E-4

Top fitness defect scores for YCL005W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_58 0957-0393 20.50 9.0 µM 1.80E-81 cell wall 7 4.19E-94
2 SGTC_2584 androsterone 13.30 48.7 µM 3.88E-35 cell wall 9 1.62E-40
3 SGTC_528 3492-0019 9.54 172.0 µM 4.99E-19 calcium & mitochondrial duress 43 7.17E-22
4 SGTC_3311 9131146 7.51 2.9 µM 1.89E-12 cell wall 38 2.97E-14
5 SGTC_951 1171-0579 7.15 82.7 µM 1.89E-11 cell wall 21 4.29E-13
6 SGTC_304 1310-0298 6.50 97.0 µM 9.56E-10 cell wall 13 4.03E-11
7 SGTC_3082 9117008 6.46 49.5 µM 1.20E-9 1 5.23E-11
8 SGTC_514 4466-0038 6.23 2.6 nM 4.39E-9 cell wall 70 2.36E-10
9 SGTC_1736 st036774 5.85 51.0 µM 3.36E-8 8 2.49E-9
10 SGTC_3266 9136860 5.74 49.5 µM 5.83E-8 cell wall 18 4.72E-9
11 SGTC_138 0850-0259 5.66 40.9 µM 8.81E-8 cell wall 117 7.61E-9
12 SGTC_3285 9112818 5.38 20.9 µM 3.49E-7 cell wall 11 3.74E-8
13 SGTC_2836 9010338 5.23 19.5 µM 7.12E-7 RPP1 & pyrimidine depletion 9 8.55E-8
14 SGTC_1847 st056231 5.06 15.0 µM 1.53E-6 13 2.07E-7
15 SGTC_863 0466-0191 4.79 129.0 µM 5.16E-6 cell wall 48 8.48E-7
16 SGTC_1255 0566-0045 4.51 221.0 µM 1.65E-5 excess fatty acid 19 3.26E-6
17 SGTC_354 2783-3500 4.47 4.9 µM 1.94E-5 cell wall 35 3.93E-6
18 SGTC_2379 9035213 4.44 200.0 µM 2.15E-5 9 4.42E-6
19 SGTC_309 k408-0051 4.38 1.5 µM 2.72E-5 38 5.81E-6
20 SGTC_413 propylparaben 4.33 100.0 µM 3.32E-5 amide catabolism 15 7.32E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.469 1.59E-183 YKL076C_d PSY1_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C
0.461 6.50E-176 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.440 1.09E-158 YJR142W_p YJR142W_p hom Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member
0.437 2.94E-156 YDL021W GPM2 hom Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event
0.434 2.92E-154 YDL066W IDP1 hom Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes
0.432 8.16E-153 YKL033W-A_p YKL033W-A_p hom Putative protein of unknown function; similar to uncharacterized proteins from other fungi
0.428 1.93E-149 YNL314W DAL82 hom Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain
0.411 5.63E-137 YLL032C YLL032C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
0.401 5.62E-130 YPL115C BEM3 hom Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly
0.398 1.99E-127 YLR331C_d JIP3_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
0.391 3.18E-123 YBL006C LDB7 hom Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions
0.391 8.91E-123 YOL003C PFA4 hom Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.380 1.83E-115 YDL203C ACK1 hom Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
0.371 3.93E-110 YPL269W KAR9 hom Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase
0.371 8.51E-110 YJL099W CHS6 hom Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication