YCL009C / ILV6

Regulatory subunit of acetolactate synthase, which catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria

Zygosity: Homozygous strain
fixedexpanded
Profile for YCL009C / ILV6

Click on Significant Values for Screen Details ID:SGTC_140|Compound:0062-0056|FD-Score:4.07|P-value:1.50E-5 ID:SGTC_189|Compound:3970-0742|FD-Score:-3.18|P-value:6.08E-4 ID:SGTC_203|Compound:k066-0004|FD-Score:3.05|P-value:8.51E-4 ID:SGTC_259|Compound:usnic acid|FD-Score:3.75|P-value:5.89E-5 ID:SGTC_600|Compound:1495-0418|FD-Score:3.17|P-value:5.64E-4 ID:SGTC_742|Compound:0kpi-0133|FD-Score:3.25|P-value:4.29E-4 ID:SGTC_789|Compound:1309-1135|FD-Score:3.15|P-value:6.14E-4 ID:SGTC_816|Compound:0325-0417|FD-Score:3.50|P-value:1.64E-4 ID:SGTC_824|Compound:1304-0008|FD-Score:3.68|P-value:7.89E-5 ID:SGTC_847|Compound:0342-0274|FD-Score:-3.38|P-value:2.91E-4 ID:SGTC_910|Compound:3454-3003|FD-Score:-3.81|P-value:5.23E-5 ID:SGTC_959|Compound:1222-0044|FD-Score:3.90|P-value:3.10E-5 ID:SGTC_976|Compound:1348-1067|FD-Score:3.50|P-value:1.60E-4 ID:SGTC_1243|Compound:0416-0003|FD-Score:4.22|P-value:7.35E-6 ID:SGTC_1249|Compound:0481-0015|FD-Score:3.45|P-value:1.94E-4 ID:SGTC_1425|Compound:4048-4433|FD-Score:3.37|P-value:2.66E-4 ID:SGTC_1507|Compound:4075-3230|FD-Score:-3.38|P-value:2.96E-4 ID:SGTC_1537|Compound:2',6'-dihydroxyflavanone|FD-Score:3.08|P-value:7.71E-4 ID:SGTC_1541|Compound:2',3',6-trimethoxyflavone|FD-Score:4.64|P-value:9.77E-7 ID:SGTC_1542|Compound:7-hydroxy-3'-methoxyflavone|FD-Score:3.40|P-value:2.44E-4 ID:SGTC_1610|Compound:st001799|FD-Score:3.61|P-value:1.06E-4 ID:SGTC_1685|Compound:st024049|FD-Score:-3.39|P-value:2.82E-4 ID:SGTC_1696|Compound:st025591|FD-Score:3.24|P-value:4.40E-4 ID:SGTC_1784|Compound:st039334|FD-Score:-3.48|P-value:1.98E-4 ID:SGTC_1908|Compound:st060353|FD-Score:5.27|P-value:3.30E-8 ID:SGTC_1916|Compound:st067606|FD-Score:6.72|P-value:2.81E-12 ID:SGTC_1955|Compound:wogonin|FD-Score:3.23|P-value:4.46E-4 ID:SGTC_1994|Compound:st072947|FD-Score:3.41|P-value:2.28E-4 ID:SGTC_2002|Compound:4023503|FD-Score:-3.30|P-value:3.99E-4 ID:SGTC_2114|Compound:5491910|FD-Score:3.70|P-value:7.18E-5 ID:SGTC_2288|Compound:7946289|FD-Score:3.40|P-value:2.40E-4 ID:SGTC_2301|Compound:7769476|FD-Score:-3.17|P-value:6.22E-4 ID:SGTC_2307|Compound:7538926|FD-Score:-4.00|P-value:2.29E-5 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:5.41|P-value:1.44E-8 ID:SGTC_2569|Compound:tetrandrine|FD-Score:3.74|P-value:6.14E-5 ID:SGTC_2748|Compound:diazoxide|FD-Score:3.66|P-value:8.43E-5 ID:SGTC_2817|Compound:7995342|FD-Score:3.31|P-value:3.41E-4 ID:SGTC_2825|Compound:7998094|FD-Score:3.01|P-value:0.00100 ID:SGTC_2854|Compound:9024559|FD-Score:3.32|P-value:3.26E-4 ID:SGTC_2858|Compound:9025781|FD-Score:3.42|P-value:2.25E-4 ID:SGTC_2890|Compound:9046729|FD-Score:5.39|P-value:1.61E-8 ID:SGTC_2939|Compound:9044578|FD-Score:-3.38|P-value:2.97E-4 ID:SGTC_2959|Compound:9079155|FD-Score:-3.30|P-value:3.91E-4 ID:SGTC_2964|Compound:9083345|FD-Score:-4.91|P-value:2.84E-7 ID:SGTC_2982|Compound:9019404|FD-Score:3.56|P-value:1.31E-4 ID:SGTC_2983|Compound:9022671|FD-Score:3.31|P-value:3.42E-4 ID:SGTC_3087|Compound:9117406|FD-Score:-3.32|P-value:3.67E-4 ID:SGTC_3118|Compound:9124250|FD-Score:3.07|P-value:7.95E-4 ID:SGTC_3196|Compound:9111891|FD-Score:4.00|P-value:2.01E-5 ID:SGTC_3233|Compound:9132340|FD-Score:3.66|P-value:8.69E-5 ID:SGTC_3306|Compound:9127676|FD-Score:-4.24|P-value:8.05E-6 ID:SGTC_140|Compound:0062-0056|FD-Score:4.07|P-value:1.50E-5 ID:SGTC_189|Compound:3970-0742|FD-Score:-3.18|P-value:6.08E-4 ID:SGTC_203|Compound:k066-0004|FD-Score:3.05|P-value:8.51E-4 ID:SGTC_259|Compound:usnic acid|FD-Score:3.75|P-value:5.89E-5 ID:SGTC_600|Compound:1495-0418|FD-Score:3.17|P-value:5.64E-4 ID:SGTC_742|Compound:0kpi-0133|FD-Score:3.25|P-value:4.29E-4 ID:SGTC_789|Compound:1309-1135|FD-Score:3.15|P-value:6.14E-4 ID:SGTC_816|Compound:0325-0417|FD-Score:3.50|P-value:1.64E-4 ID:SGTC_824|Compound:1304-0008|FD-Score:3.68|P-value:7.89E-5 ID:SGTC_847|Compound:0342-0274|FD-Score:-3.38|P-value:2.91E-4 ID:SGTC_910|Compound:3454-3003|FD-Score:-3.81|P-value:5.23E-5 ID:SGTC_959|Compound:1222-0044|FD-Score:3.90|P-value:3.10E-5 ID:SGTC_976|Compound:1348-1067|FD-Score:3.50|P-value:1.60E-4 ID:SGTC_1243|Compound:0416-0003|FD-Score:4.22|P-value:7.35E-6 ID:SGTC_1249|Compound:0481-0015|FD-Score:3.45|P-value:1.94E-4 ID:SGTC_1425|Compound:4048-4433|FD-Score:3.37|P-value:2.66E-4 ID:SGTC_1507|Compound:4075-3230|FD-Score:-3.38|P-value:2.96E-4 ID:SGTC_1537|Compound:2',6'-dihydroxyflavanone|FD-Score:3.08|P-value:7.71E-4 ID:SGTC_1541|Compound:2',3',6-trimethoxyflavone|FD-Score:4.64|P-value:9.77E-7 ID:SGTC_1542|Compound:7-hydroxy-3'-methoxyflavone|FD-Score:3.40|P-value:2.44E-4 ID:SGTC_1610|Compound:st001799|FD-Score:3.61|P-value:1.06E-4 ID:SGTC_1685|Compound:st024049|FD-Score:-3.39|P-value:2.82E-4 ID:SGTC_1696|Compound:st025591|FD-Score:3.24|P-value:4.40E-4 ID:SGTC_1784|Compound:st039334|FD-Score:-3.48|P-value:1.98E-4 ID:SGTC_1908|Compound:st060353|FD-Score:5.27|P-value:3.30E-8 ID:SGTC_1916|Compound:st067606|FD-Score:6.72|P-value:2.81E-12 ID:SGTC_1955|Compound:wogonin|FD-Score:3.23|P-value:4.46E-4 ID:SGTC_1994|Compound:st072947|FD-Score:3.41|P-value:2.28E-4 ID:SGTC_2002|Compound:4023503|FD-Score:-3.30|P-value:3.99E-4 ID:SGTC_2114|Compound:5491910|FD-Score:3.70|P-value:7.18E-5 ID:SGTC_2288|Compound:7946289|FD-Score:3.40|P-value:2.40E-4 ID:SGTC_2301|Compound:7769476|FD-Score:-3.17|P-value:6.22E-4 ID:SGTC_2307|Compound:7538926|FD-Score:-4.00|P-value:2.29E-5 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:5.41|P-value:1.44E-8 ID:SGTC_2569|Compound:tetrandrine|FD-Score:3.74|P-value:6.14E-5 ID:SGTC_2748|Compound:diazoxide|FD-Score:3.66|P-value:8.43E-5 ID:SGTC_2817|Compound:7995342|FD-Score:3.31|P-value:3.41E-4 ID:SGTC_2825|Compound:7998094|FD-Score:3.01|P-value:0.00100 ID:SGTC_2854|Compound:9024559|FD-Score:3.32|P-value:3.26E-4 ID:SGTC_2858|Compound:9025781|FD-Score:3.42|P-value:2.25E-4 ID:SGTC_2890|Compound:9046729|FD-Score:5.39|P-value:1.61E-8 ID:SGTC_2939|Compound:9044578|FD-Score:-3.38|P-value:2.97E-4 ID:SGTC_2959|Compound:9079155|FD-Score:-3.30|P-value:3.91E-4 ID:SGTC_2964|Compound:9083345|FD-Score:-4.91|P-value:2.84E-7 ID:SGTC_2982|Compound:9019404|FD-Score:3.56|P-value:1.31E-4 ID:SGTC_2983|Compound:9022671|FD-Score:3.31|P-value:3.42E-4 ID:SGTC_3087|Compound:9117406|FD-Score:-3.32|P-value:3.67E-4 ID:SGTC_3118|Compound:9124250|FD-Score:3.07|P-value:7.95E-4 ID:SGTC_3196|Compound:9111891|FD-Score:4.00|P-value:2.01E-5 ID:SGTC_3233|Compound:9132340|FD-Score:3.66|P-value:8.69E-5 ID:SGTC_3306|Compound:9127676|FD-Score:-4.24|P-value:8.05E-6

Top fitness defect scores for YCL009C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
1 SGTC_1916 st067606 6.72 49.2 µM 2.81E-12 5 8.86E-12
2 SGTC_2567 aureobasidin a 5.41 60.0 nM 1.44E-8 54 3.10E-8
3 SGTC_2890 9046729 5.39 58.4 µM 1.61E-8 Golgi 10 3.45E-8
4 SGTC_1908 st060353 5.27 56.5 µM 3.30E-8 RPP1 & pyrimidine depletion 16 6.86E-8
5 SGTC_1541 2',3',6-trimethoxyflavone 4.64 64.0 µM 9.77E-7 8 1.75E-6
6 SGTC_1243 0416-0003 4.22 5.7 µM 7.35E-6 PDR1 22 1.20E-5
7 SGTC_140 0062-0056 4.07 502.5 µM 1.50E-5 21 2.38E-5
8 SGTC_3196 9111891 4.00 49.5 µM 2.01E-5 mitochondrial processes 15 3.15E-5
9 SGTC_959 1222-0044 3.90 107.0 µM 3.10E-5 20 4.76E-5
10 SGTC_259 usnic acid 3.75 108.9 µM 5.89E-5 24 8.78E-5
11 SGTC_2569 tetrandrine 3.74 100.0 µM 6.14E-5 25 9.15E-5
12 SGTC_2114 5491910 3.70 81.2 µM 7.18E-5 17 1.06E-4
13 SGTC_824 1304-0008 3.68 100.0 µM 7.89E-5 DNA damage response 44 1.16E-4
14 SGTC_2748 diazoxide 3.66 86.7 µM 8.43E-5 10 1.24E-4
15 SGTC_3233 9132340 3.66 49.5 µM 8.69E-5 27 1.27E-4
16 SGTC_1610 st001799 3.61 48.1 µM 1.06E-4 18 1.54E-4
17 SGTC_2982 9019404 3.56 26.0 µM 1.31E-4 29 1.89E-4
18 SGTC_976 1348-1067 3.50 47.6 µM 1.60E-4 60S ribosome export 48 2.28E-4
19 SGTC_816 0325-0417 3.50 81.3 µM 1.64E-4 20 2.34E-4
20 SGTC_1249 0481-0015 3.45 10.2 µM 1.94E-4 41 2.75E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.167 2.65E-22 YKL004W AUR1 het Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance
0.128 9.64E-14 YDR367W KEI1 het Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene
0.112 7.60E-11 YNR074C AIF1 hom Mitochondrial cell death effector that translocates to the nucleus in response to apoptotic stimuli, homolog of mammalian Apoptosis-Inducing Factor, putative reductase
0.102 3.17E-9 YKL151C YKL151C hom NADHX dehydratase; converts (S)-NADHX to NADH in an ATP-dependent manner; homologous to Carkd in mammals, and the C-terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; YKL151C promoter contains STREs (stress response elements) and expression is induced by heat shock or methyl methanesulfonate; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress
0.100 6.85E-9 YLR079W SIC1 hom Cyclin-dependent kinase inhibitor (CKI); inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1
0.094 5.47E-8 YDR425W SNX41 hom Sorting nexin, involved in the retrieval of late-Golgi SNAREs from the post-Golgi endosome to the trans-Golgi network; interacts with Snx4p
0.093 7.38E-8 YOR147W MDM32 hom Mitochondrial inner membrane protein with similarity to Mdm31p, required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MDM10, MDM12, and MDM34
0.092 8.54E-8 YBR233W PBP2 hom RNA binding protein; has similarity to mammalian heterogeneous nuclear RNP K protein, involved in the regulation of telomere position effect and telomere length; relative distribution to the nucleus increases upon DNA replication stress
0.092 1.04E-7 YCL040W GLK1 hom Glucokinase; catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources; GLK1 has a paralog, EMI2, that arose from the whole genome duplication
0.091 1.11E-7 YDL192W ARF1 hom ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication
0.091 1.19E-7 YLL037W_d YLL037W_d het Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential PRP19 gene encoding an RNA splicing factor
0.087 4.03E-7 YOL019W-A_p YOL019W-A_p hom Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
0.087 4.97E-7 YBR244W GPX2 hom Phospholipid hydroperoxide glutathione peroxidase; protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress; induced by glucose starvation; protein abundance increases in response to DNA replication stress
0.086 6.91E-7 YLR285C-A_p YLR285C-A_p hom Putative protein of unknown function; identified by fungal homology and RT-PCR
0.085 7.69E-7 YIL044C AGE2 hom ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in Trans-Golgi-Network (TGN) transport; contains C2C2H2 cysteine/histidine motif