YCL017C / NFS1

Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria

Zygosity: Heterozygous strain
fixedexpanded
Profile for YCL017C / NFS1

Click on Significant Values for Screen Details ID:SGTC_3|Compound:0833-0587|FD-Score:4.12|P-value:2.95E-5 ID:SGTC_31|Compound:0508-1974|FD-Score:3.46|P-value:3.76E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:-3.21|P-value:8.51E-4 ID:SGTC_68|Compound:0986-0284|FD-Score:4.80|P-value:1.41E-6 ID:SGTC_73|Compound:0327-0325|FD-Score:3.32|P-value:6.10E-4 ID:SGTC_141|Compound:0443-0274|FD-Score:-5.68|P-value:1.38E-8 ID:SGTC_145|Compound:3737-0113|FD-Score:4.43|P-value:7.69E-6 ID:SGTC_158|Compound:k038-0087|FD-Score:-3.19|P-value:9.04E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:3.84|P-value:8.95E-5 ID:SGTC_214|Compound:4373-3496|FD-Score:3.51|P-value:3.14E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:-4.92|P-value:7.61E-7 ID:SGTC_252|Compound:miconazole|FD-Score:3.81|P-value:1.01E-4 ID:SGTC_256|Compound:clotrimazole|FD-Score:3.30|P-value:6.59E-4 ID:SGTC_302|Compound:1094-0063|FD-Score:3.32|P-value:5.97E-4 ID:SGTC_401|Compound:neomycin|FD-Score:-3.27|P-value:6.79E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-4.13|P-value:2.67E-5 ID:SGTC_525|Compound:k781-2675|FD-Score:4.10|P-value:3.24E-5 ID:SGTC_547|Compound:1120-0019|FD-Score:-3.66|P-value:1.69E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:-3.55|P-value:2.58E-4 ID:SGTC_576|Compound:1073-0113|FD-Score:5.03|P-value:4.72E-7 ID:SGTC_733|Compound:4069-0007|FD-Score:-4.64|P-value:2.88E-6 ID:SGTC_741|Compound:4582-0002|FD-Score:-3.33|P-value:5.54E-4 ID:SGTC_743|Compound:1275-0778|FD-Score:4.83|P-value:1.26E-6 ID:SGTC_745|Compound:0469-0705|FD-Score:-4.83|P-value:1.14E-6 ID:SGTC_747|Compound:3448-8087|FD-Score:4.90|P-value:8.77E-7 ID:SGTC_795|Compound:0685-0265|FD-Score:4.20|P-value:2.10E-5 ID:SGTC_805|Compound:3852-0218|FD-Score:-3.84|P-value:8.51E-5 ID:SGTC_965|Compound:1291-5240|FD-Score:-3.65|P-value:1.75E-4 ID:SGTC_1006|Compound:4099-6547|FD-Score:3.65|P-value:1.85E-4 ID:SGTC_1019|Compound:4262-0212|FD-Score:4.81|P-value:1.37E-6 ID:SGTC_1044|Compound:1222-0021|FD-Score:4.71|P-value:2.23E-6 ID:SGTC_1072|Compound:oxiconazole|FD-Score:3.72|P-value:1.41E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:7.62|P-value:5.30E-14 ID:SGTC_1133|Compound:3473-0247|FD-Score:3.67|P-value:1.75E-4 ID:SGTC_1230|Compound:0170-0365|FD-Score:-3.52|P-value:2.90E-4 ID:SGTC_1259|Compound:0671-0132|FD-Score:-3.57|P-value:2.41E-4 ID:SGTC_1423|Compound:4048-0112|FD-Score:4.11|P-value:3.11E-5 ID:SGTC_1667|Compound:st014849|FD-Score:4.61|P-value:3.48E-6 ID:SGTC_1670|Compound:st016595|FD-Score:3.74|P-value:1.35E-4 ID:SGTC_1707|Compound:st032323|FD-Score:3.75|P-value:1.28E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:5.82|P-value:7.03E-9 ID:SGTC_1765|Compound:st044815|FD-Score:-4.98|P-value:5.59E-7 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:10.10|P-value:5.16E-23 ID:SGTC_1787|Compound:fluconazole|FD-Score:5.67|P-value:1.61E-8 ID:SGTC_1802|Compound:st050112|FD-Score:4.00|P-value:4.79E-5 ID:SGTC_1884|Compound:5633444|FD-Score:4.29|P-value:1.43E-5 ID:SGTC_1893|Compound:st059353|FD-Score:3.68|P-value:1.67E-4 ID:SGTC_2049|Compound:5262831|FD-Score:3.69|P-value:1.63E-4 ID:SGTC_2050|Compound:5263056|FD-Score:-4.53|P-value:4.65E-6 ID:SGTC_2160|Compound:5660466|FD-Score:-3.94|P-value:5.80E-5 ID:SGTC_2163|Compound:5671233|FD-Score:4.25|P-value:1.72E-5 ID:SGTC_2348|Compound:9022543|FD-Score:3.26|P-value:7.39E-4 ID:SGTC_2522|Compound:biochanin a|FD-Score:5.22|P-value:1.75E-7 ID:SGTC_2534|Compound:helenine|FD-Score:3.49|P-value:3.39E-4 ID:SGTC_2548|Compound:3',4'-didesmethyl-5-deshydroxy-3'-ethoxyscleroin|FD-Score:5.38|P-value:7.83E-8 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:4.20|P-value:2.08E-5 ID:SGTC_2574|Compound:2',4'-dihydroxychalcone|FD-Score:3.55|P-value:2.68E-4 ID:SGTC_2624|Compound:nerol|FD-Score:3.45|P-value:3.88E-4 ID:SGTC_2671|Compound:danthron|FD-Score:3.48|P-value:3.52E-4 ID:SGTC_2748|Compound:diazoxide|FD-Score:5.61|P-value:2.18E-8 ID:SGTC_2778|Compound:tioconazole|FD-Score:4.43|P-value:7.68E-6 ID:SGTC_2852|Compound:9023854|FD-Score:5.46|P-value:5.12E-8 ID:SGTC_2853|Compound:9015042|FD-Score:3.40|P-value:4.68E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-3.47|P-value:3.40E-4 ID:SGTC_2910|Compound:7948405|FD-Score:-3.32|P-value:5.89E-4 ID:SGTC_2958|Compound:9077254|FD-Score:4.24|P-value:1.79E-5 ID:SGTC_2965|Compound:9082772|FD-Score:-7.40|P-value:2.37E-13 ID:SGTC_3037|Compound:9091026|FD-Score:4.78|P-value:1.61E-6 ID:SGTC_3038|Compound:9091290|FD-Score:-3.64|P-value:1.84E-4 ID:SGTC_3144|Compound:9087057|FD-Score:3.36|P-value:5.29E-4 ID:SGTC_3210|Compound:9128157|FD-Score:3.22|P-value:8.57E-4 ID:SGTC_3254|Compound:9136651|FD-Score:4.55|P-value:4.54E-6 ID:SGTC_3264|Compound:9137442|FD-Score:3.60|P-value:2.29E-4 ID:SGTC_3|Compound:0833-0587|FD-Score:4.12|P-value:2.95E-5 ID:SGTC_31|Compound:0508-1974|FD-Score:3.46|P-value:3.76E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:-3.21|P-value:8.51E-4 ID:SGTC_68|Compound:0986-0284|FD-Score:4.80|P-value:1.41E-6 ID:SGTC_73|Compound:0327-0325|FD-Score:3.32|P-value:6.10E-4 ID:SGTC_141|Compound:0443-0274|FD-Score:-5.68|P-value:1.38E-8 ID:SGTC_145|Compound:3737-0113|FD-Score:4.43|P-value:7.69E-6 ID:SGTC_158|Compound:k038-0087|FD-Score:-3.19|P-value:9.04E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:3.84|P-value:8.95E-5 ID:SGTC_214|Compound:4373-3496|FD-Score:3.51|P-value:3.14E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:-4.92|P-value:7.61E-7 ID:SGTC_252|Compound:miconazole|FD-Score:3.81|P-value:1.01E-4 ID:SGTC_256|Compound:clotrimazole|FD-Score:3.30|P-value:6.59E-4 ID:SGTC_302|Compound:1094-0063|FD-Score:3.32|P-value:5.97E-4 ID:SGTC_401|Compound:neomycin|FD-Score:-3.27|P-value:6.79E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-4.13|P-value:2.67E-5 ID:SGTC_525|Compound:k781-2675|FD-Score:4.10|P-value:3.24E-5 ID:SGTC_547|Compound:1120-0019|FD-Score:-3.66|P-value:1.69E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:-3.55|P-value:2.58E-4 ID:SGTC_576|Compound:1073-0113|FD-Score:5.03|P-value:4.72E-7 ID:SGTC_733|Compound:4069-0007|FD-Score:-4.64|P-value:2.88E-6 ID:SGTC_741|Compound:4582-0002|FD-Score:-3.33|P-value:5.54E-4 ID:SGTC_743|Compound:1275-0778|FD-Score:4.83|P-value:1.26E-6 ID:SGTC_745|Compound:0469-0705|FD-Score:-4.83|P-value:1.14E-6 ID:SGTC_747|Compound:3448-8087|FD-Score:4.90|P-value:8.77E-7 ID:SGTC_795|Compound:0685-0265|FD-Score:4.20|P-value:2.10E-5 ID:SGTC_805|Compound:3852-0218|FD-Score:-3.84|P-value:8.51E-5 ID:SGTC_965|Compound:1291-5240|FD-Score:-3.65|P-value:1.75E-4 ID:SGTC_1006|Compound:4099-6547|FD-Score:3.65|P-value:1.85E-4 ID:SGTC_1019|Compound:4262-0212|FD-Score:4.81|P-value:1.37E-6 ID:SGTC_1044|Compound:1222-0021|FD-Score:4.71|P-value:2.23E-6 ID:SGTC_1072|Compound:oxiconazole|FD-Score:3.72|P-value:1.41E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:7.62|P-value:5.30E-14 ID:SGTC_1133|Compound:3473-0247|FD-Score:3.67|P-value:1.75E-4 ID:SGTC_1230|Compound:0170-0365|FD-Score:-3.52|P-value:2.90E-4 ID:SGTC_1259|Compound:0671-0132|FD-Score:-3.57|P-value:2.41E-4 ID:SGTC_1423|Compound:4048-0112|FD-Score:4.11|P-value:3.11E-5 ID:SGTC_1667|Compound:st014849|FD-Score:4.61|P-value:3.48E-6 ID:SGTC_1670|Compound:st016595|FD-Score:3.74|P-value:1.35E-4 ID:SGTC_1707|Compound:st032323|FD-Score:3.75|P-value:1.28E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:5.82|P-value:7.03E-9 ID:SGTC_1765|Compound:st044815|FD-Score:-4.98|P-value:5.59E-7 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:10.10|P-value:5.16E-23 ID:SGTC_1787|Compound:fluconazole|FD-Score:5.67|P-value:1.61E-8 ID:SGTC_1802|Compound:st050112|FD-Score:4.00|P-value:4.79E-5 ID:SGTC_1884|Compound:5633444|FD-Score:4.29|P-value:1.43E-5 ID:SGTC_1893|Compound:st059353|FD-Score:3.68|P-value:1.67E-4 ID:SGTC_2049|Compound:5262831|FD-Score:3.69|P-value:1.63E-4 ID:SGTC_2050|Compound:5263056|FD-Score:-4.53|P-value:4.65E-6 ID:SGTC_2160|Compound:5660466|FD-Score:-3.94|P-value:5.80E-5 ID:SGTC_2163|Compound:5671233|FD-Score:4.25|P-value:1.72E-5 ID:SGTC_2348|Compound:9022543|FD-Score:3.26|P-value:7.39E-4 ID:SGTC_2522|Compound:biochanin a|FD-Score:5.22|P-value:1.75E-7 ID:SGTC_2534|Compound:helenine|FD-Score:3.49|P-value:3.39E-4 ID:SGTC_2548|Compound:3',4'-didesmethyl-5-deshydroxy-3'-ethoxyscleroin|FD-Score:5.38|P-value:7.83E-8 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:4.20|P-value:2.08E-5 ID:SGTC_2574|Compound:2',4'-dihydroxychalcone|FD-Score:3.55|P-value:2.68E-4 ID:SGTC_2624|Compound:nerol|FD-Score:3.45|P-value:3.88E-4 ID:SGTC_2671|Compound:danthron|FD-Score:3.48|P-value:3.52E-4 ID:SGTC_2748|Compound:diazoxide|FD-Score:5.61|P-value:2.18E-8 ID:SGTC_2778|Compound:tioconazole|FD-Score:4.43|P-value:7.68E-6 ID:SGTC_2852|Compound:9023854|FD-Score:5.46|P-value:5.12E-8 ID:SGTC_2853|Compound:9015042|FD-Score:3.40|P-value:4.68E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-3.47|P-value:3.40E-4 ID:SGTC_2910|Compound:7948405|FD-Score:-3.32|P-value:5.89E-4 ID:SGTC_2958|Compound:9077254|FD-Score:4.24|P-value:1.79E-5 ID:SGTC_2965|Compound:9082772|FD-Score:-7.40|P-value:2.37E-13 ID:SGTC_3037|Compound:9091026|FD-Score:4.78|P-value:1.61E-6 ID:SGTC_3038|Compound:9091290|FD-Score:-3.64|P-value:1.84E-4 ID:SGTC_3144|Compound:9087057|FD-Score:3.36|P-value:5.29E-4 ID:SGTC_3210|Compound:9128157|FD-Score:3.22|P-value:8.57E-4 ID:SGTC_3254|Compound:9136651|FD-Score:4.55|P-value:4.54E-6 ID:SGTC_3264|Compound:9137442|FD-Score:3.60|P-value:2.29E-4

Top fitness defect scores for YCL017C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1785 bathophenanthroline 10.10 24.0 µM 5.16E-23 heme requiring 1 4.52E-24 2.14
2 SGTC_1083 didemethylchlorpromazine sulfoxide 7.62 707.4 nM 5.30E-14 redox potentiating 10 1.28E-14 0.18
3 SGTC_1718 roemerine 5.82 11.9 µM 7.03E-9 redox potentiating 8 3.02E-9 0.19
4 SGTC_1787 fluconazole 5.67 80.0 µM 1.61E-8 iron homeostasis 4 7.20E-9 0.55
5 SGTC_2748 diazoxide 5.61 86.7 µM 2.18E-8 1 9.86E-9 1.51
6 SGTC_2852 9023854 5.46 52.0 µM 5.12E-8 2 2.42E-8 0.64
7 SGTC_2548 3',4'-didesmethyl-5-deshydroxy-3'-ethoxyscleroin 5.38 19.9 µM 7.83E-8 redox potentiating 1 3.78E-8 1.33
8 SGTC_2522 biochanin a 5.22 4.9 µM 1.75E-7 RNA pol III & RNase P/MRP 8 8.79E-8 0.50
9 SGTC_576 1073-0113 5.03 22.7 µM 4.72E-7 redox potentiating 25 2.48E-7 0.16
10 SGTC_747 3448-8087 4.90 38.7 µM 8.77E-7 5 4.76E-7 0.03
11 SGTC_743 1275-0778 4.83 158.0 µM 1.26E-6 excess fatty acid 8 6.94E-7 0.21
12 SGTC_1019 4262-0212 4.81 3.4 µM 1.37E-6 ergosterol depletion effects on membrane 8 7.60E-7 0.06
13 SGTC_68 0986-0284 4.80 82.8 µM 1.41E-6 6 7.82E-7 0.54
14 SGTC_3037 9091026 4.78 49.5 µM 1.61E-6 NEO1-PIK1 17 8.96E-7 0.40
15 SGTC_1044 1222-0021 4.71 1.8 µM 2.23E-6 heme biosynthesis & mitochondrial translocase 29 1.26E-6 0.37
16 SGTC_1667 st014849 4.61 34.6 µM 3.48E-6 Golgi 2 2.02E-6 0.20
17 SGTC_3254 9136651 4.55 49.5 µM 4.54E-6 DNA damage response 1 2.67E-6 0.25
18 SGTC_2778 tioconazole 4.43 65.1 nM 7.68E-6 ergosterol depletion effects on membrane 23 4.62E-6 0.07
19 SGTC_145 3737-0113 4.43 38.4 µM 7.69E-6 DNA intercalators 39 4.63E-6 0.12
20 SGTC_1884 5633444 4.29 25.0 µM 1.43E-5 redox potentiating 13 8.86E-6 0.23

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.277 3.55E-60 YPL252C YAH1 het Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin
0.262 9.75E-54 YPL251W_d YPL251W_d het Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C
0.198 4.35E-31 YDR376W ARH1 het Oxidoreductase of the mitochondrial inner membrane, involved in cytoplasmic and mitochondrial iron homeostasis and required for activity of Fe-S cluster-containing enzymes; one of the few mitochondrial proteins essential for viability
0.163 1.76E-21 YDR431W_d YDR431W_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.130 4.82E-14 YHR042W NCP1 het NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately regulated with Erg11p
0.129 5.86E-14 YIL068C SEC6 het Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p
0.121 1.77E-12 YMR211W DML1 het Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
0.117 9.34E-12 YDR224C HTB1 het Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation
0.113 5.50E-11 YJR102C VPS25 hom Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome
0.110 1.74E-10 YPL135W ISU1 hom Conserved protein of the mitochondrial matrix, performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); isu1 isu2 double mutant is inviable
0.110 1.81E-10 YDR478W SNM1 het Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP
0.109 2.68E-10 YMR218C TRS130 het One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in ER to Golgi membrane traffic; mutation activates transcription of OCH1; mutants are defective in cytoplasm-to-vacuole targeting (Cvt) pathway and starvation-induced autophagy
0.109 2.71E-10 YOR275C RIM20 hom Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation
0.105 9.88E-10 YJL081C ARP4 het Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes
0.103 2.28E-9 YMR043W MCM1 het Transcription factor involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes