HIPHOP chemogenomics database

YCL017C / NFS1

Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria

Zygosity: Heterozygous strain

fixedexpanded
Profile for YCL017C / NFS1

Click on Significant Values for Screen Details

Top fitness defect scores for YCL017C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_1785	bathophenanthroline	10.10	24.0 µM	5.16E-23	heme requiring	1	4.52E-24	2.14
2	SGTC_1083	didemethylchlorpromazine sulfoxide	7.62	707.4 nM	5.30E-14	redox potentiating	10	1.28E-14	0.18
3	SGTC_1718	roemerine	5.82	11.9 µM	7.03E-9	redox potentiating	8	3.02E-9	0.19
4	SGTC_1787	fluconazole	5.67	80.0 µM	1.61E-8	iron homeostasis	4	7.20E-9	0.55
5	SGTC_2748	diazoxide	5.61	86.7 µM	2.18E-8		1	9.86E-9	1.51
6	SGTC_2852	9023854	5.46	52.0 µM	5.12E-8		2	2.42E-8	0.64
7	SGTC_2548	3',4'-didesmethyl-5-deshydroxy-3'-ethoxyscleroin	5.38	19.9 µM	7.83E-8	redox potentiating	1	3.78E-8	1.33
8	SGTC_2522	biochanin a	5.22	4.9 µM	1.75E-7	RNA pol III & RNase P/MRP	8	8.79E-8	0.50
9	SGTC_576	1073-0113	5.03	22.7 µM	4.72E-7	redox potentiating	25	2.48E-7	0.16
10	SGTC_747	3448-8087	4.90	38.7 µM	8.77E-7		5	4.76E-7	0.03
11	SGTC_743	1275-0778	4.83	158.0 µM	1.26E-6	excess fatty acid	8	6.94E-7	0.21
12	SGTC_1019	4262-0212	4.81	3.4 µM	1.37E-6	ergosterol depletion effects on membrane	8	7.60E-7	0.06
13	SGTC_68	0986-0284	4.80	82.8 µM	1.41E-6		6	7.82E-7	0.54
14	SGTC_3037	9091026	4.78	49.5 µM	1.61E-6	NEO1-PIK1	17	8.96E-7	0.40
15	SGTC_1044	1222-0021	4.71	1.8 µM	2.23E-6	heme biosynthesis & mitochondrial translocase	29	1.26E-6	0.37
16	SGTC_1667	st014849	4.61	34.6 µM	3.48E-6	Golgi	2	2.02E-6	0.20
17	SGTC_3254	9136651	4.55	49.5 µM	4.54E-6	DNA damage response	1	2.67E-6	0.25
18	SGTC_2778	tioconazole	4.43	65.1 nM	7.68E-6	ergosterol depletion effects on membrane	23	4.62E-6	0.07
19	SGTC_145	3737-0113	4.43	38.4 µM	7.69E-6	DNA intercalators	39	4.63E-6	0.12
20	SGTC_1884	5633444	4.29	25.0 µM	1.43E-5	redox potentiating	13	8.86E-6	0.23

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.277	3.55E-60	YPL252C	YAH1	het	Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin
0.262	9.75E-54	YPL251W_d	YPL251W_d	het	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C
0.198	4.35E-31	YDR376W	ARH1	het	Oxidoreductase of the mitochondrial inner membrane, involved in cytoplasmic and mitochondrial iron homeostasis and required for activity of Fe-S cluster-containing enzymes; one of the few mitochondrial proteins essential for viability
0.163	1.76E-21	YDR431W_d	YDR431W_d	hom	Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.130	4.82E-14	YHR042W	NCP1	het	NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately regulated with Erg11p
0.129	5.86E-14	YIL068C	SEC6	het	Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p
0.121	1.77E-12	YMR211W	DML1	het	Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
0.117	9.34E-12	YDR224C	HTB1	het	Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation
0.113	5.50E-11	YJR102C	VPS25	hom	Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome
0.110	1.74E-10	YPL135W	ISU1	hom	Conserved protein of the mitochondrial matrix, performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); isu1 isu2 double mutant is inviable
0.110	1.81E-10	YDR478W	SNM1	het	Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP
0.109	2.68E-10	YMR218C	TRS130	het	One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in ER to Golgi membrane traffic; mutation activates transcription of OCH1; mutants are defective in cytoplasm-to-vacuole targeting (Cvt) pathway and starvation-induced autophagy
0.109	2.71E-10	YOR275C	RIM20	hom	Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation
0.105	9.88E-10	YJL081C	ARP4	het	Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes
0.103	2.28E-9	YMR043W	MCM1	het	Transcription factor involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes