YCL025C / AGP1

Low-affinity amino acid permease with broad substrate range; involved in uptake of asparagine, glutamine, and other amino acids; expression regulated by SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); AGP1 has a paralog, GNP1, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YCL025C / AGP1

Click on Significant Values for Screen Details ID:SGTC_92|Compound:0063-0024|FD-Score:-2.76|P-value:7.38E-4 ID:SGTC_208|Compound:k072-0098|FD-Score:-3.18|P-value:1.23E-4 ID:SGTC_333|Compound:3486-0131|FD-Score:3.46|P-value:2.18E-5 ID:SGTC_350|Compound:k064-0020|FD-Score:2.65|P-value:8.29E-4 ID:SGTC_362|Compound:0310-0100|FD-Score:3.23|P-value:6.55E-5 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:3.04|P-value:1.56E-4 ID:SGTC_522|Compound:1273-0010|FD-Score:3.52|P-value:1.55E-5 ID:SGTC_535|Compound:2293-4129|FD-Score:3.18|P-value:8.31E-5 ID:SGTC_566|Compound:0958-0068|FD-Score:3.06|P-value:1.44E-4 ID:SGTC_622|Compound:1348-1566|FD-Score:3.03|P-value:1.68E-4 ID:SGTC_632|Compound:0781-2353|FD-Score:2.88|P-value:3.17E-4 ID:SGTC_1007|Compound:4101-0031|FD-Score:3.11|P-value:1.17E-4 ID:SGTC_1158|Compound:4092-0494|FD-Score:3.32|P-value:4.30E-5 ID:SGTC_1299|Compound:1079-1439|FD-Score:3.23|P-value:6.49E-5 ID:SGTC_1334|Compound:1486-0610|FD-Score:2.83|P-value:3.90E-4 ID:SGTC_1409|Compound:3909-8004|FD-Score:3.21|P-value:7.42E-5 ID:SGTC_1424|Compound:4048-1941|FD-Score:2.81|P-value:4.27E-4 ID:SGTC_1479|Compound:0549-0158|FD-Score:2.89|P-value:3.04E-4 ID:SGTC_1530|Compound:rhein|FD-Score:3.11|P-value:1.17E-4 ID:SGTC_1541|Compound:2',3',6-trimethoxyflavone|FD-Score:3.50|P-value:1.75E-5 ID:SGTC_1544|Compound:7-methoxy-4-methylcoumarin|FD-Score:3.08|P-value:1.35E-4 ID:SGTC_1655|Compound:st012546|FD-Score:4.50|P-value:5.74E-8 ID:SGTC_1836|Compound:st055642|FD-Score:3.07|P-value:1.39E-4 ID:SGTC_1851|Compound:st056236|FD-Score:3.31|P-value:4.49E-5 ID:SGTC_1970|Compound:st070122|FD-Score:-2.72|P-value:8.74E-4 ID:SGTC_2038|Compound:4100038|FD-Score:2.73|P-value:6.08E-4 ID:SGTC_2327|Compound:6189656|FD-Score:2.65|P-value:8.24E-4 ID:SGTC_2395|Compound:5646831|FD-Score:3.92|P-value:1.84E-6 ID:SGTC_2463|Compound:5473429|FD-Score:3.97|P-value:1.39E-6 ID:SGTC_2559|Compound:tetrahydrosappanone a trimethyl ether|FD-Score:-2.85|P-value:5.07E-4 ID:SGTC_2592|Compound:piperlongumine|FD-Score:-3.79|P-value:5.87E-6 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:-3.98|P-value:2.04E-6 ID:SGTC_2601|Compound:securinine|FD-Score:4.86|P-value:5.04E-9 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:4.82|P-value:6.72E-9 ID:SGTC_2647|Compound:atorvastatin|FD-Score:2.62|P-value:9.55E-4 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:2.87|P-value:3.40E-4 ID:SGTC_2710|Compound:st078562|FD-Score:3.86|P-value:2.62E-6 ID:SGTC_2790|Compound:5423970|FD-Score:2.94|P-value:2.49E-4 ID:SGTC_2799|Compound:7812892|FD-Score:-3.13|P-value:1.52E-4 ID:SGTC_2888|Compound:9058547|FD-Score:3.25|P-value:5.91E-5 ID:SGTC_2902|Compound:5787006|FD-Score:2.76|P-value:5.39E-4 ID:SGTC_2906|Compound:7926292|FD-Score:2.70|P-value:6.89E-4 ID:SGTC_2994|Compound:9053520|FD-Score:6.09|P-value:3.98E-13 ID:SGTC_2995|Compound:9067767|FD-Score:2.72|P-value:6.21E-4 ID:SGTC_3045|Compound:9092950|FD-Score:2.76|P-value:5.34E-4 ID:SGTC_3056|Compound:9094900|FD-Score:-2.69|P-value:9.63E-4 ID:SGTC_3211|Compound:9128781|FD-Score:3.66|P-value:7.81E-6 ID:SGTC_92|Compound:0063-0024|FD-Score:-2.76|P-value:7.38E-4 ID:SGTC_208|Compound:k072-0098|FD-Score:-3.18|P-value:1.23E-4 ID:SGTC_333|Compound:3486-0131|FD-Score:3.46|P-value:2.18E-5 ID:SGTC_350|Compound:k064-0020|FD-Score:2.65|P-value:8.29E-4 ID:SGTC_362|Compound:0310-0100|FD-Score:3.23|P-value:6.55E-5 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:3.04|P-value:1.56E-4 ID:SGTC_522|Compound:1273-0010|FD-Score:3.52|P-value:1.55E-5 ID:SGTC_535|Compound:2293-4129|FD-Score:3.18|P-value:8.31E-5 ID:SGTC_566|Compound:0958-0068|FD-Score:3.06|P-value:1.44E-4 ID:SGTC_622|Compound:1348-1566|FD-Score:3.03|P-value:1.68E-4 ID:SGTC_632|Compound:0781-2353|FD-Score:2.88|P-value:3.17E-4 ID:SGTC_1007|Compound:4101-0031|FD-Score:3.11|P-value:1.17E-4 ID:SGTC_1158|Compound:4092-0494|FD-Score:3.32|P-value:4.30E-5 ID:SGTC_1299|Compound:1079-1439|FD-Score:3.23|P-value:6.49E-5 ID:SGTC_1334|Compound:1486-0610|FD-Score:2.83|P-value:3.90E-4 ID:SGTC_1409|Compound:3909-8004|FD-Score:3.21|P-value:7.42E-5 ID:SGTC_1424|Compound:4048-1941|FD-Score:2.81|P-value:4.27E-4 ID:SGTC_1479|Compound:0549-0158|FD-Score:2.89|P-value:3.04E-4 ID:SGTC_1530|Compound:rhein|FD-Score:3.11|P-value:1.17E-4 ID:SGTC_1541|Compound:2',3',6-trimethoxyflavone|FD-Score:3.50|P-value:1.75E-5 ID:SGTC_1544|Compound:7-methoxy-4-methylcoumarin|FD-Score:3.08|P-value:1.35E-4 ID:SGTC_1655|Compound:st012546|FD-Score:4.50|P-value:5.74E-8 ID:SGTC_1836|Compound:st055642|FD-Score:3.07|P-value:1.39E-4 ID:SGTC_1851|Compound:st056236|FD-Score:3.31|P-value:4.49E-5 ID:SGTC_1970|Compound:st070122|FD-Score:-2.72|P-value:8.74E-4 ID:SGTC_2038|Compound:4100038|FD-Score:2.73|P-value:6.08E-4 ID:SGTC_2327|Compound:6189656|FD-Score:2.65|P-value:8.24E-4 ID:SGTC_2395|Compound:5646831|FD-Score:3.92|P-value:1.84E-6 ID:SGTC_2463|Compound:5473429|FD-Score:3.97|P-value:1.39E-6 ID:SGTC_2559|Compound:tetrahydrosappanone a trimethyl ether|FD-Score:-2.85|P-value:5.07E-4 ID:SGTC_2592|Compound:piperlongumine|FD-Score:-3.79|P-value:5.87E-6 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:-3.98|P-value:2.04E-6 ID:SGTC_2601|Compound:securinine|FD-Score:4.86|P-value:5.04E-9 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:4.82|P-value:6.72E-9 ID:SGTC_2647|Compound:atorvastatin|FD-Score:2.62|P-value:9.55E-4 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:2.87|P-value:3.40E-4 ID:SGTC_2710|Compound:st078562|FD-Score:3.86|P-value:2.62E-6 ID:SGTC_2790|Compound:5423970|FD-Score:2.94|P-value:2.49E-4 ID:SGTC_2799|Compound:7812892|FD-Score:-3.13|P-value:1.52E-4 ID:SGTC_2888|Compound:9058547|FD-Score:3.25|P-value:5.91E-5 ID:SGTC_2902|Compound:5787006|FD-Score:2.76|P-value:5.39E-4 ID:SGTC_2906|Compound:7926292|FD-Score:2.70|P-value:6.89E-4 ID:SGTC_2994|Compound:9053520|FD-Score:6.09|P-value:3.98E-13 ID:SGTC_2995|Compound:9067767|FD-Score:2.72|P-value:6.21E-4 ID:SGTC_3045|Compound:9092950|FD-Score:2.76|P-value:5.34E-4 ID:SGTC_3056|Compound:9094900|FD-Score:-2.69|P-value:9.63E-4 ID:SGTC_3211|Compound:9128781|FD-Score:3.66|P-value:7.81E-6

Top fitness defect scores for YCL025C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2994 9053520 6.09 71.4 µM 3.98E-13 RPP1 & pyrimidine depletion 5 5.73E-10
2 SGTC_2601 securinine 4.86 100.0 µM 5.04E-9 13 5.82E-7
3 SGTC_2637 3-alpha-acetoxydihydrodeoxygedunin 4.82 100.0 µM 6.72E-9 9 7.18E-7
4 SGTC_1655 st012546 4.50 55.1 µM 5.74E-8 17 3.46E-6
5 SGTC_2463 5473429 3.97 200.0 µM 1.39E-6 ERG2 14 3.59E-5
6 SGTC_2395 5646831 3.92 180.2 µM 1.84E-6 22 4.42E-5
7 SGTC_2710 st078562 3.86 53.7 µM 2.62E-6 17 5.74E-5
8 SGTC_3211 9128781 3.66 49.5 µM 7.81E-6 78 1.28E-4
9 SGTC_522 1273-0010 3.52 11.6 µM 1.55E-5 60S ribosome export 33 2.12E-4
10 SGTC_1541 2',3',6-trimethoxyflavone 3.50 64.0 µM 1.75E-5 25 2.33E-4
11 SGTC_333 3486-0131 3.46 40.1 µM 2.18E-5 amide catabolism 17 2.73E-4
12 SGTC_1158 4092-0494 3.32 91.8 µM 4.30E-5 33 4.51E-4
13 SGTC_1851 st056236 3.31 18.9 µM 4.49E-5 36 4.65E-4
14 SGTC_2888 9058547 3.25 13.0 µM 5.91E-5 22 5.70E-4
15 SGTC_1299 1079-1439 3.23 3.7 µM 6.49E-5 TSC3-RPN4 38 6.10E-4
16 SGTC_362 0310-0100 3.23 9.0 µM 6.55E-5 RPP1 & pyrimidine depletion 69 6.14E-4
17 SGTC_1409 3909-8004 3.21 61.9 µM 7.42E-5 54 6.73E-4
18 SGTC_535 2293-4129 3.18 48.2 µM 8.31E-5 NEO1 109 7.32E-4
19 SGTC_1007 4101-0031 3.11 70.0 µM 1.17E-4 46 9.44E-4
20 SGTC_1530 rhein 3.11 70.4 µM 1.17E-4 50 9.44E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.114 4.04E-11 YER069W ARG5,6 hom Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine
0.105 9.14E-10 YBR144C_d YBR144C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR144C is not an essential gene
0.100 6.51E-9 YOL086W-A MHF1 hom Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM, a Fanconi anemia complementation group protein, to stabilize and remodel blocked replication forks and repair damaged DNA; mhf1 srs2 double mutants are MMS hypersensitive; ortholog of human centromere constitutive-associated network (CCAN) subunit CENP-S, also known as MHF1
0.096 2.42E-8 YOR171C LCB4 hom Sphingoid long-chain base kinase, responsible for synthesis of long-chain base phosphates, which function as signaling molecules, regulates synthesis of ceramide from exogenous long-chain bases, localizes to the Golgi and late endosomes
0.090 1.73E-7 YJL066C MPM1 hom Mitochondrial intermembrane space protein of unknown function
0.090 1.84E-7 YMR167W MLH1 hom Protein required for mismatch repair in mitosis and meiosis as well as crossing over during meiosis; forms a complex with Pms1p and Msh2p-Msh3p during mismatch repair; human homolog is associated with hereditary non-polyposis colon cancer
0.086 6.46E-7 YGR122C-A_d YGR122C-A_d hom Dubious open reading frame unlikely to encode a functional protein, similar to YLR334C and YOL106W
0.084 1.16E-6 YER168C CCA1 het ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites
0.084 1.23E-6 YOL140W ARG8 hom Acetylornithine aminotransferase, catalyzes the fourth step in the biosynthesis of the arginine precursor ornithine
0.083 1.66E-6 YLR298C YHC1 het Component of the U1 snRNP complex required for pre-mRNA splicing; putative ortholog of human U1C protein, which is involved in formation of a complex between U1 snRNP and the pre-mRNA 5' splice site
0.082 2.24E-6 YPL081W RPS9A hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9A has a paralog, RPS9B, that arose from the whole genome duplication
0.081 2.70E-6 YLR042C_p YLR042C_p hom Protein of unknown function; localizes to the cytoplasm; YLL042C is not an essential gene
0.079 4.73E-6 YPR062W FCY1 hom Cytosine deaminase, zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU)
0.076 1.18E-5 YHL022C SPO11 hom Meiosis-specific protein that initiates meiotic recombination by catalyzing the formation of double-strand breaks in DNA via a transesterification reaction; required for homologous chromosome pairing and synaptonemal complex formation
0.074 1.70E-5 YIR019C FLO11 hom GPI-anchored cell surface glycoprotein (flocculin); required for pseudohyphal formation, invasive growth, flocculation, and biofilms; transcriptionally regulated by the MAPK pathway (via Ste12p and Tec1p) and the cAMP pathway (via Flo8p); required for the formation of fibrous interconnections between cells in a colony of a wild S. cerevisiae strain