YCL027W / FUS1

Membrane protein localized to the shmoo tip, required for cell fusion; expression regulated by mating pheromone; proposed to coordinate signaling, fusion, and polarization events required for fusion; potential Cdc28p substrate

Zygosity: Homozygous strain
fixedexpanded
Profile for YCL027W / FUS1

Click on Significant Values for Screen Details ID:SGTC_64|Compound:000l-0018|FD-Score:4.04|P-value:1.74E-6 ID:SGTC_98|Compound:0074-0023|FD-Score:3.60|P-value:1.79E-5 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:-2.86|P-value:5.49E-4 ID:SGTC_280|Compound:3448-3607|FD-Score:3.93|P-value:3.11E-6 ID:SGTC_281|Compound:1672-3440|FD-Score:4.96|P-value:6.19E-9 ID:SGTC_688|Compound:0527-0193|FD-Score:3.63|P-value:1.52E-5 ID:SGTC_869|Compound:0564-0080|FD-Score:4.00|P-value:2.19E-6 ID:SGTC_891|Compound:0929-0073|FD-Score:3.60|P-value:1.74E-5 ID:SGTC_1168|Compound:k815-0004|FD-Score:-3.10|P-value:2.06E-4 ID:SGTC_1171|Compound:0368-0077|FD-Score:-4.92|P-value:9.26E-9 ID:SGTC_1177|Compound:0983-0204|FD-Score:3.22|P-value:1.06E-4 ID:SGTC_1201|Compound:1889-3372|FD-Score:3.01|P-value:2.68E-4 ID:SGTC_1239|Compound:0337-0584|FD-Score:2.83|P-value:5.54E-4 ID:SGTC_1269|Compound:0831-0796|FD-Score:2.73|P-value:8.39E-4 ID:SGTC_1384|Compound:3089-0840|FD-Score:3.19|P-value:1.20E-4 ID:SGTC_1537|Compound:2',6'-dihydroxyflavanone|FD-Score:3.80|P-value:6.42E-6 ID:SGTC_1567|Compound:st069303|FD-Score:-2.87|P-value:5.37E-4 ID:SGTC_1604|Compound:st002052|FD-Score:-4.29|P-value:4.81E-7 ID:SGTC_1623|Compound:st003709|FD-Score:-3.38|P-value:5.86E-5 ID:SGTC_1688|Compound:st021091|FD-Score:2.74|P-value:8.17E-4 ID:SGTC_1702|Compound:st027038|FD-Score:3.74|P-value:8.41E-6 ID:SGTC_1751|Compound:st044839|FD-Score:2.79|P-value:6.72E-4 ID:SGTC_1784|Compound:st039334|FD-Score:2.69|P-value:9.82E-4 ID:SGTC_1866|Compound:st057638|FD-Score:3.22|P-value:1.05E-4 ID:SGTC_2036|Compound:5160441|FD-Score:4.30|P-value:4.01E-7 ID:SGTC_2063|Compound:5246145|FD-Score:2.88|P-value:4.57E-4 ID:SGTC_2264|Compound:7948757|FD-Score:-2.85|P-value:5.75E-4 ID:SGTC_2467|Compound:5474540|FD-Score:-3.26|P-value:1.01E-4 ID:SGTC_2470|Compound:5664817|FD-Score:-2.77|P-value:8.01E-4 ID:SGTC_2730|Compound:imipramine|FD-Score:2.70|P-value:9.61E-4 ID:SGTC_2935|Compound:9038016|FD-Score:2.84|P-value:5.40E-4 ID:SGTC_3305|Compound:9127027|FD-Score:-3.10|P-value:2.02E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:4.04|P-value:1.74E-6 ID:SGTC_98|Compound:0074-0023|FD-Score:3.60|P-value:1.79E-5 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:-2.86|P-value:5.49E-4 ID:SGTC_280|Compound:3448-3607|FD-Score:3.93|P-value:3.11E-6 ID:SGTC_281|Compound:1672-3440|FD-Score:4.96|P-value:6.19E-9 ID:SGTC_688|Compound:0527-0193|FD-Score:3.63|P-value:1.52E-5 ID:SGTC_869|Compound:0564-0080|FD-Score:4.00|P-value:2.19E-6 ID:SGTC_891|Compound:0929-0073|FD-Score:3.60|P-value:1.74E-5 ID:SGTC_1168|Compound:k815-0004|FD-Score:-3.10|P-value:2.06E-4 ID:SGTC_1171|Compound:0368-0077|FD-Score:-4.92|P-value:9.26E-9 ID:SGTC_1177|Compound:0983-0204|FD-Score:3.22|P-value:1.06E-4 ID:SGTC_1201|Compound:1889-3372|FD-Score:3.01|P-value:2.68E-4 ID:SGTC_1239|Compound:0337-0584|FD-Score:2.83|P-value:5.54E-4 ID:SGTC_1269|Compound:0831-0796|FD-Score:2.73|P-value:8.39E-4 ID:SGTC_1384|Compound:3089-0840|FD-Score:3.19|P-value:1.20E-4 ID:SGTC_1537|Compound:2',6'-dihydroxyflavanone|FD-Score:3.80|P-value:6.42E-6 ID:SGTC_1567|Compound:st069303|FD-Score:-2.87|P-value:5.37E-4 ID:SGTC_1604|Compound:st002052|FD-Score:-4.29|P-value:4.81E-7 ID:SGTC_1623|Compound:st003709|FD-Score:-3.38|P-value:5.86E-5 ID:SGTC_1688|Compound:st021091|FD-Score:2.74|P-value:8.17E-4 ID:SGTC_1702|Compound:st027038|FD-Score:3.74|P-value:8.41E-6 ID:SGTC_1751|Compound:st044839|FD-Score:2.79|P-value:6.72E-4 ID:SGTC_1784|Compound:st039334|FD-Score:2.69|P-value:9.82E-4 ID:SGTC_1866|Compound:st057638|FD-Score:3.22|P-value:1.05E-4 ID:SGTC_2036|Compound:5160441|FD-Score:4.30|P-value:4.01E-7 ID:SGTC_2063|Compound:5246145|FD-Score:2.88|P-value:4.57E-4 ID:SGTC_2264|Compound:7948757|FD-Score:-2.85|P-value:5.75E-4 ID:SGTC_2467|Compound:5474540|FD-Score:-3.26|P-value:1.01E-4 ID:SGTC_2470|Compound:5664817|FD-Score:-2.77|P-value:8.01E-4 ID:SGTC_2730|Compound:imipramine|FD-Score:2.70|P-value:9.61E-4 ID:SGTC_2935|Compound:9038016|FD-Score:2.84|P-value:5.40E-4 ID:SGTC_3305|Compound:9127027|FD-Score:-3.10|P-value:2.02E-4

Top fitness defect scores for YCL027W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_281 1672-3440 4.96 14.9 µM 6.19E-9 Golgi 3 3.55E-7
2 SGTC_2036 5160441 4.30 105.0 µM 4.01E-7 NEO1 71 8.71E-6
3 SGTC_64 000l-0018 4.04 272.1 µM 1.74E-6 ubiquinone biosynthesis & proteasome 145 2.69E-5
4 SGTC_869 0564-0080 4.00 44.1 µM 2.19E-6 22 3.21E-5
5 SGTC_280 3448-3607 3.93 3.0 µM 3.11E-6 60S ribosome export 10 4.21E-5
6 SGTC_1537 2',6'-dihydroxyflavanone 3.80 78.0 µM 6.42E-6 20 7.34E-5
7 SGTC_1702 st027038 3.74 51.0 µM 8.41E-6 16 9.04E-5
8 SGTC_688 0527-0193 3.63 473.0 µM 1.52E-5 fatty acid desaturase (OLE1) 19 1.43E-4
9 SGTC_891 0929-0073 3.60 133.0 µM 1.74E-5 33 1.58E-4
10 SGTC_98 0074-0023 3.60 107.7 µM 1.79E-5 Golgi 53 1.62E-4
11 SGTC_1866 st057638 3.22 64.0 µM 1.05E-4 29 6.33E-4
12 SGTC_1177 0983-0204 3.22 23.6 µM 1.06E-4 34 6.38E-4
13 SGTC_1384 3089-0840 3.19 101.0 µM 1.20E-4 39 7.04E-4
14 SGTC_1201 1889-3372 3.01 45.9 µM 2.68E-4 Golgi 33 0.00131
15 SGTC_2063 5246145 2.88 200.0 µM 4.57E-4 79 0.00198
16 SGTC_2935 9038016 2.84 30.0 µM 5.40E-4 77 0.00225
17 SGTC_1239 0337-0584 2.83 35.4 µM 5.54E-4 unfolded protein response 127 0.00229
18 SGTC_1751 st044839 2.79 27.2 µM 6.72E-4 Golgi 49 0.00266
19 SGTC_1688 st021091 2.74 57.6 µM 8.17E-4 36 0.00310
20 SGTC_1269 0831-0796 2.73 3.0 µM 8.39E-4 60S ribosome export 65 0.00316

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.120 3.65E-12 YLR021W IRC25 hom Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions
0.115 2.92E-11 YBL045C COR1 hom Core subunit of the ubiquinol-cytochrome c reductase complex (bc1 complex), which is a component of the mitochondrial inner membrane electron transport chain
0.113 4.79E-11 YBL041W PRE7 het Beta 6 subunit of the 20S proteasome
0.112 8.42E-11 YMR022W UBC7 hom Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly
0.110 1.96E-10 YLR199C PBA1 hom Protein involved in 20S proteasome assembly; forms a heterodimer with Add66p that binds to proteasome precursors; similar to human PAC1 constituent of the PAC1-PAC2 complex involved in proteasome assembly
0.109 2.35E-10 YPL144W POC4 hom Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions
0.109 2.56E-10 YER094C PUP3 het Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10
0.108 3.15E-10 YOR301W RAX1 hom Protein involved in bud site selection during bipolar budding; localization requires Rax2p; has similarity to members of the insulin-related peptide superfamily
0.101 4.32E-9 YML014W TRM9 hom tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses
0.098 1.39E-8 YMR314W PRE5 het Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
0.097 1.72E-8 YPR146C_d YPR146C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.093 8.35E-8 YFL040W_p YFL040W_p hom Putative transporter, member of the sugar porter family; YFL040W is not an essential gene
0.092 8.59E-8 YBR106W PHO88 hom Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations
0.089 2.37E-7 YNL111C CYB5 hom Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation
0.089 2.50E-7 YLR239C LIP2 hom Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups