YCL030C / HIS4

Multifunctional enzyme containing phosphoribosyl-ATP pyrophosphatase, phosphoribosyl-AMP cyclohydrolase, and histidinol dehydrogenase activities; catalyzes the second, third, ninth and tenth steps in histidine biosynthesis

Zygosity: Homozygous strain
fixedexpanded
Profile for YCL030C / HIS4

Click on Significant Values for Screen Details ID:SGTC_319|Compound:1348-1596|FD-Score:3.89|P-value:5.50E-5 ID:SGTC_333|Compound:3486-0131|FD-Score:3.15|P-value:8.67E-4 ID:SGTC_337|Compound:1315-0038|FD-Score:3.32|P-value:4.87E-4 ID:SGTC_402|Compound:epigallocatechin gallate|FD-Score:4.74|P-value:1.19E-6 ID:SGTC_574|Compound:1315-0397|FD-Score:3.49|P-value:2.57E-4 ID:SGTC_579|Compound:4491-1009|FD-Score:3.77|P-value:8.73E-5 ID:SGTC_644|Compound:1498-1197|FD-Score:3.61|P-value:1.63E-4 ID:SGTC_925|Compound:2064-1272|FD-Score:-3.70|P-value:9.97E-5 ID:SGTC_936|Compound:3005-4620|FD-Score:3.59|P-value:1.77E-4 ID:SGTC_1205|Compound:4161-2785|FD-Score:-3.17|P-value:7.06E-4 ID:SGTC_1587|Compound:estradiol valerate|FD-Score:3.73|P-value:1.05E-4 ID:SGTC_1774|Compound:st048371|FD-Score:3.54|P-value:2.13E-4 ID:SGTC_1775|Compound:st081588|FD-Score:4.49|P-value:3.94E-6 ID:SGTC_1795|Compound:st049545|FD-Score:-4.37|P-value:5.80E-6 ID:SGTC_1910|Compound:st062352|FD-Score:-3.23|P-value:5.80E-4 ID:SGTC_1915|Compound:st067105|FD-Score:4.25|P-value:1.15E-5 ID:SGTC_1967|Compound:st070177|FD-Score:3.50|P-value:2.49E-4 ID:SGTC_1977|Compound:st070720|FD-Score:3.25|P-value:6.21E-4 ID:SGTC_2191|Compound:5911152|FD-Score:4.11|P-value:2.20E-5 ID:SGTC_2213|Compound:7173844|FD-Score:3.65|P-value:1.44E-4 ID:SGTC_2233|Compound:sulbentine|FD-Score:3.35|P-value:4.31E-4 ID:SGTC_2330|Compound:7979373|FD-Score:5.45|P-value:2.82E-8 ID:SGTC_2340|Compound:7987427|FD-Score:3.82|P-value:7.14E-5 ID:SGTC_2422|Compound:chlorphenesin carbamate|FD-Score:5.94|P-value:1.62E-9 ID:SGTC_2432|Compound:5630864|FD-Score:6.10|P-value:5.92E-10 ID:SGTC_2503|Compound:beta-caryophyllene alcohol|FD-Score:-3.34|P-value:3.96E-4 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:3.29|P-value:5.43E-4 ID:SGTC_2623|Compound:humulene|FD-Score:4.22|P-value:1.35E-5 ID:SGTC_2789|Compound:5281112|FD-Score:3.28|P-value:5.67E-4 ID:SGTC_2870|Compound:9038528|FD-Score:-3.18|P-value:6.86E-4 ID:SGTC_3031|Compound:9088762|FD-Score:3.80|P-value:7.99E-5 ID:SGTC_3044|Compound:9093193|FD-Score:-3.71|P-value:9.65E-5 ID:SGTC_3066|Compound:9117245|FD-Score:4.90|P-value:5.37E-7 ID:SGTC_3072|Compound:9116419|FD-Score:3.84|P-value:6.71E-5 ID:SGTC_3078|Compound:9116413|FD-Score:3.30|P-value:5.11E-4 ID:SGTC_3085|Compound:9117376|FD-Score:4.25|P-value:1.16E-5 ID:SGTC_3127|Compound:9126946|FD-Score:-3.35|P-value:3.71E-4 ID:SGTC_3134|Compound:9127454|FD-Score:4.61|P-value:2.20E-6 ID:SGTC_319|Compound:1348-1596|FD-Score:3.89|P-value:5.50E-5 ID:SGTC_333|Compound:3486-0131|FD-Score:3.15|P-value:8.67E-4 ID:SGTC_337|Compound:1315-0038|FD-Score:3.32|P-value:4.87E-4 ID:SGTC_402|Compound:epigallocatechin gallate|FD-Score:4.74|P-value:1.19E-6 ID:SGTC_574|Compound:1315-0397|FD-Score:3.49|P-value:2.57E-4 ID:SGTC_579|Compound:4491-1009|FD-Score:3.77|P-value:8.73E-5 ID:SGTC_644|Compound:1498-1197|FD-Score:3.61|P-value:1.63E-4 ID:SGTC_925|Compound:2064-1272|FD-Score:-3.70|P-value:9.97E-5 ID:SGTC_936|Compound:3005-4620|FD-Score:3.59|P-value:1.77E-4 ID:SGTC_1205|Compound:4161-2785|FD-Score:-3.17|P-value:7.06E-4 ID:SGTC_1587|Compound:estradiol valerate|FD-Score:3.73|P-value:1.05E-4 ID:SGTC_1774|Compound:st048371|FD-Score:3.54|P-value:2.13E-4 ID:SGTC_1775|Compound:st081588|FD-Score:4.49|P-value:3.94E-6 ID:SGTC_1795|Compound:st049545|FD-Score:-4.37|P-value:5.80E-6 ID:SGTC_1910|Compound:st062352|FD-Score:-3.23|P-value:5.80E-4 ID:SGTC_1915|Compound:st067105|FD-Score:4.25|P-value:1.15E-5 ID:SGTC_1967|Compound:st070177|FD-Score:3.50|P-value:2.49E-4 ID:SGTC_1977|Compound:st070720|FD-Score:3.25|P-value:6.21E-4 ID:SGTC_2191|Compound:5911152|FD-Score:4.11|P-value:2.20E-5 ID:SGTC_2213|Compound:7173844|FD-Score:3.65|P-value:1.44E-4 ID:SGTC_2233|Compound:sulbentine|FD-Score:3.35|P-value:4.31E-4 ID:SGTC_2330|Compound:7979373|FD-Score:5.45|P-value:2.82E-8 ID:SGTC_2340|Compound:7987427|FD-Score:3.82|P-value:7.14E-5 ID:SGTC_2422|Compound:chlorphenesin carbamate|FD-Score:5.94|P-value:1.62E-9 ID:SGTC_2432|Compound:5630864|FD-Score:6.10|P-value:5.92E-10 ID:SGTC_2503|Compound:beta-caryophyllene alcohol|FD-Score:-3.34|P-value:3.96E-4 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:3.29|P-value:5.43E-4 ID:SGTC_2623|Compound:humulene|FD-Score:4.22|P-value:1.35E-5 ID:SGTC_2789|Compound:5281112|FD-Score:3.28|P-value:5.67E-4 ID:SGTC_2870|Compound:9038528|FD-Score:-3.18|P-value:6.86E-4 ID:SGTC_3031|Compound:9088762|FD-Score:3.80|P-value:7.99E-5 ID:SGTC_3044|Compound:9093193|FD-Score:-3.71|P-value:9.65E-5 ID:SGTC_3066|Compound:9117245|FD-Score:4.90|P-value:5.37E-7 ID:SGTC_3072|Compound:9116419|FD-Score:3.84|P-value:6.71E-5 ID:SGTC_3078|Compound:9116413|FD-Score:3.30|P-value:5.11E-4 ID:SGTC_3085|Compound:9117376|FD-Score:4.25|P-value:1.16E-5 ID:SGTC_3127|Compound:9126946|FD-Score:-3.35|P-value:3.71E-4 ID:SGTC_3134|Compound:9127454|FD-Score:4.61|P-value:2.20E-6

Top fitness defect scores for YCL030C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
1 SGTC_2432 5630864 6.10 12.2 µM 5.92E-10 1 5.20E-10
2 SGTC_2422 chlorphenesin carbamate 5.94 384.2 µM 1.62E-9 5 1.43E-9
3 SGTC_2330 7979373 5.45 200.0 µM 2.82E-8 60S ribosome export 15 2.51E-8
4 SGTC_3066 9117245 4.90 49.5 µM 5.37E-7 4 4.83E-7
5 SGTC_402 epigallocatechin gallate 4.74 10.0 µM 1.19E-6 8 1.07E-6
6 SGTC_3134 9127454 4.61 49.5 µM 2.20E-6 7 1.99E-6
7 SGTC_1775 st081588 4.49 35.2 µM 3.94E-6 60S ribosome export 14 3.58E-6
8 SGTC_1915 st067105 4.25 30.6 µM 1.15E-5 RNA pol III & RNase P/MRP 36 1.05E-5
9 SGTC_3085 9117376 4.25 49.5 µM 1.16E-5 5 1.05E-5
10 SGTC_2623 humulene 4.22 100.0 µM 1.35E-5 13 1.23E-5
11 SGTC_2191 5911152 4.11 200.0 µM 2.20E-5 10 2.01E-5
12 SGTC_319 1348-1596 3.89 45.5 µM 5.50E-5 RSC & ERG11 20 5.06E-5
13 SGTC_3072 9116419 3.84 49.5 µM 6.71E-5 ERG2 20 6.17E-5
14 SGTC_2340 7987427 3.82 183.4 µM 7.14E-5 38 6.57E-5
15 SGTC_3031 9088762 3.80 49.5 µM 7.99E-5 26 7.35E-5
16 SGTC_579 4491-1009 3.77 110.0 µM 8.73E-5 60S ribosome export 27 8.04E-5
17 SGTC_1587 estradiol valerate 3.73 56.1 µM 1.05E-4 15 9.65E-5
18 SGTC_2213 7173844 3.65 200.0 µM 1.44E-4 17 1.33E-4
19 SGTC_644 1498-1197 3.61 63.3 µM 1.63E-4 32 1.51E-4
20 SGTC_936 3005-4620 3.59 401.0 µM 1.77E-4 35 1.64E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.092 8.96E-8 YBR152W SPP381 het mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p
0.092 1.06E-7 YDR225W HTA1 hom Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p
0.090 1.74E-7 YFL034C-A RPL22B hom Ribosomal 60S subunit protein L22B; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22B has a paralog, RPL22A, that arose from the whole genome duplication
0.083 1.63E-6 YCR075C ERS1 hom Protein with similarity to human cystinosin, which is a H(+)-driven transporter involved in L-cystine export from lysosomes and implicated in the disease cystinosis; contains seven transmembrane domains
0.081 2.35E-6 YOR207C RET1 het Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
0.081 2.61E-6 YOR256C TRE2 het Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication
0.078 5.61E-6 YLL016W SDC25 hom Non-essential Ras guanine nucleotide exchange factor (GEF); localized to the membrane; expressed in poor nutrient conditions and on nonfermentable carbon sources; contains a stop codon in S288C, full-length gene includes YLL017W; SDC25 has a paralog, CDC25, that arose from the whole genome duplication
0.078 5.91E-6 YKL088W CAB3 het Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC
0.075 1.26E-5 YBL078C ATG8 hom Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis
0.075 1.33E-5 YEL056W HAT2 hom Subunit of the Hat1p-Hat2p histone acetyltransferase complex; required for high affinity binding of the complex to free histone H4, thereby enhancing Hat1p activity; similar to human RbAp46 and 48; has a role in telomeric silencing
0.075 1.55E-5 YEL011W GLC3 hom Glycogen branching enzyme, involved in glycogen accumulation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
0.071 3.52E-5 YPR052C NHP6A hom High-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to chromosomes; functionally redundant with Nhp6Bp; homologous to mammalian HMGB1 and HMGB2; NHP6A has a paralog, NHP6B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
0.070 4.76E-5 YDL150W RPC53 het RNA polymerase III subunit C53
0.070 5.47E-5 YNL260C LTO1 het Essential protein that forms a complex with Rli1p and Yae1p; ortholog of human ORAOV1, which is overexpressed in solid tumors; inviability of null mutant under standard conditions is complemented by overexpression of ORAOV1; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species
0.069 6.79E-5 YER118C SHO1 hom Transmembrane osmosensor involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p