YCL031C / RRP7

Essential protein involved in rRNA processing and ribosome biogenesis; protein abundance increases in response to DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YCL031C / RRP7

Click on Significant Values for Screen Details ID:SGTC_57|Compound:miconazole|FD-Score:9.97|P-value:2.78E-22 ID:SGTC_122|Compound:0236-0016|FD-Score:4.22|P-value:2.39E-5 ID:SGTC_134|Compound:0269-0018|FD-Score:4.32|P-value:1.56E-5 ID:SGTC_168|Compound:3970-0862|FD-Score:5.44|P-value:7.74E-8 ID:SGTC_226|Compound:1,3-diallylurea|FD-Score:4.35|P-value:1.34E-5 ID:SGTC_252|Compound:miconazole|FD-Score:5.90|P-value:5.97E-9 ID:SGTC_256|Compound:clotrimazole|FD-Score:7.49|P-value:2.34E-13 ID:SGTC_365|Compound:0121-0052|FD-Score:3.53|P-value:3.39E-4 ID:SGTC_415|Compound:dibenzyl ketone|FD-Score:3.24|P-value:9.21E-4 ID:SGTC_445|Compound:e6 berbamine|FD-Score:-4.43|P-value:7.91E-6 ID:SGTC_451|Compound:chloral hydrate|FD-Score:-5.10|P-value:3.56E-7 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:4.93|P-value:9.65E-7 ID:SGTC_479|Compound:n-linoleoylglycine|FD-Score:3.21|P-value:9.91E-4 ID:SGTC_481|Compound:bay k-8644|FD-Score:3.67|P-value:2.07E-4 ID:SGTC_539|Compound:4859-0462|FD-Score:5.67|P-value:2.15E-8 ID:SGTC_541|Compound:4513-1321|FD-Score:3.38|P-value:5.60E-4 ID:SGTC_601|Compound:0986-0029|FD-Score:4.95|P-value:8.90E-7 ID:SGTC_682|Compound:0kpi-0099|FD-Score:7.44|P-value:3.22E-13 ID:SGTC_695|Compound:3505-6186|FD-Score:3.62|P-value:2.41E-4 ID:SGTC_829|Compound:1635-0120|FD-Score:3.64|P-value:2.24E-4 ID:SGTC_913|Compound:3487-1245|FD-Score:-3.23|P-value:8.26E-4 ID:SGTC_1018|Compound:4259-0013|FD-Score:5.67|P-value:2.17E-8 ID:SGTC_1072|Compound:oxiconazole|FD-Score:4.24|P-value:2.19E-5 ID:SGTC_1133|Compound:3473-0247|FD-Score:4.58|P-value:4.97E-6 ID:SGTC_1194|Compound:1488-0194|FD-Score:-3.64|P-value:1.92E-4 ID:SGTC_1229|Compound:0139-0193|FD-Score:3.30|P-value:7.55E-4 ID:SGTC_1453|Compound:4237-0541|FD-Score:3.60|P-value:2.59E-4 ID:SGTC_1469|Compound:k284-2957|FD-Score:4.20|P-value:2.61E-5 ID:SGTC_1722|Compound:st034304|FD-Score:5.03|P-value:5.97E-7 ID:SGTC_1850|Compound:st056238|FD-Score:4.16|P-value:3.01E-5 ID:SGTC_2022|Compound:5136654|FD-Score:-3.98|P-value:5.39E-5 ID:SGTC_2069|Compound:5228469|FD-Score:4.18|P-value:2.78E-5 ID:SGTC_2073|Compound:5212005|FD-Score:-3.77|P-value:1.22E-4 ID:SGTC_2093|Compound:5331342|FD-Score:5.18|P-value:2.94E-7 ID:SGTC_2122|Compound:5258151|FD-Score:4.11|P-value:3.75E-5 ID:SGTC_2150|Compound:5556613|FD-Score:3.48|P-value:4.07E-4 ID:SGTC_2153|Compound:5569715|FD-Score:3.25|P-value:8.64E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:4.84|P-value:1.54E-6 ID:SGTC_2496|Compound:diffractaic acid|FD-Score:-3.57|P-value:2.47E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:3.62|P-value:2.47E-4 ID:SGTC_2612|Compound:dictamnine|FD-Score:8.24|P-value:8.63E-16 ID:SGTC_2714|Compound:st079313|FD-Score:-3.36|P-value:5.24E-4 ID:SGTC_2787|Compound:5284540|FD-Score:-3.51|P-value:3.12E-4 ID:SGTC_2790|Compound:5423970|FD-Score:-3.51|P-value:3.11E-4 ID:SGTC_2841|Compound:9004761|FD-Score:5.29|P-value:1.65E-7 ID:SGTC_2850|Compound:9015429|FD-Score:3.53|P-value:3.33E-4 ID:SGTC_2854|Compound:9024559|FD-Score:-3.33|P-value:5.84E-4 ID:SGTC_2977|Compound:9011197|FD-Score:6.04|P-value:2.73E-9 ID:SGTC_2983|Compound:9022671|FD-Score:4.70|P-value:2.85E-6 ID:SGTC_2987|Compound:9018338|FD-Score:4.93|P-value:9.85E-7 ID:SGTC_3054|Compound:9094397|FD-Score:-3.20|P-value:8.95E-4 ID:SGTC_3152|Compound:9099253|FD-Score:3.26|P-value:8.55E-4 ID:SGTC_3187|Compound:9109394|FD-Score:4.54|P-value:6.01E-6 ID:SGTC_3318|Compound:9139014|FD-Score:4.39|P-value:1.17E-5 ID:SGTC_57|Compound:miconazole|FD-Score:9.97|P-value:2.78E-22 ID:SGTC_122|Compound:0236-0016|FD-Score:4.22|P-value:2.39E-5 ID:SGTC_134|Compound:0269-0018|FD-Score:4.32|P-value:1.56E-5 ID:SGTC_168|Compound:3970-0862|FD-Score:5.44|P-value:7.74E-8 ID:SGTC_226|Compound:1,3-diallylurea|FD-Score:4.35|P-value:1.34E-5 ID:SGTC_252|Compound:miconazole|FD-Score:5.90|P-value:5.97E-9 ID:SGTC_256|Compound:clotrimazole|FD-Score:7.49|P-value:2.34E-13 ID:SGTC_365|Compound:0121-0052|FD-Score:3.53|P-value:3.39E-4 ID:SGTC_415|Compound:dibenzyl ketone|FD-Score:3.24|P-value:9.21E-4 ID:SGTC_445|Compound:e6 berbamine|FD-Score:-4.43|P-value:7.91E-6 ID:SGTC_451|Compound:chloral hydrate|FD-Score:-5.10|P-value:3.56E-7 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:4.93|P-value:9.65E-7 ID:SGTC_479|Compound:n-linoleoylglycine|FD-Score:3.21|P-value:9.91E-4 ID:SGTC_481|Compound:bay k-8644|FD-Score:3.67|P-value:2.07E-4 ID:SGTC_539|Compound:4859-0462|FD-Score:5.67|P-value:2.15E-8 ID:SGTC_541|Compound:4513-1321|FD-Score:3.38|P-value:5.60E-4 ID:SGTC_601|Compound:0986-0029|FD-Score:4.95|P-value:8.90E-7 ID:SGTC_682|Compound:0kpi-0099|FD-Score:7.44|P-value:3.22E-13 ID:SGTC_695|Compound:3505-6186|FD-Score:3.62|P-value:2.41E-4 ID:SGTC_829|Compound:1635-0120|FD-Score:3.64|P-value:2.24E-4 ID:SGTC_913|Compound:3487-1245|FD-Score:-3.23|P-value:8.26E-4 ID:SGTC_1018|Compound:4259-0013|FD-Score:5.67|P-value:2.17E-8 ID:SGTC_1072|Compound:oxiconazole|FD-Score:4.24|P-value:2.19E-5 ID:SGTC_1133|Compound:3473-0247|FD-Score:4.58|P-value:4.97E-6 ID:SGTC_1194|Compound:1488-0194|FD-Score:-3.64|P-value:1.92E-4 ID:SGTC_1229|Compound:0139-0193|FD-Score:3.30|P-value:7.55E-4 ID:SGTC_1453|Compound:4237-0541|FD-Score:3.60|P-value:2.59E-4 ID:SGTC_1469|Compound:k284-2957|FD-Score:4.20|P-value:2.61E-5 ID:SGTC_1722|Compound:st034304|FD-Score:5.03|P-value:5.97E-7 ID:SGTC_1850|Compound:st056238|FD-Score:4.16|P-value:3.01E-5 ID:SGTC_2022|Compound:5136654|FD-Score:-3.98|P-value:5.39E-5 ID:SGTC_2069|Compound:5228469|FD-Score:4.18|P-value:2.78E-5 ID:SGTC_2073|Compound:5212005|FD-Score:-3.77|P-value:1.22E-4 ID:SGTC_2093|Compound:5331342|FD-Score:5.18|P-value:2.94E-7 ID:SGTC_2122|Compound:5258151|FD-Score:4.11|P-value:3.75E-5 ID:SGTC_2150|Compound:5556613|FD-Score:3.48|P-value:4.07E-4 ID:SGTC_2153|Compound:5569715|FD-Score:3.25|P-value:8.64E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:4.84|P-value:1.54E-6 ID:SGTC_2496|Compound:diffractaic acid|FD-Score:-3.57|P-value:2.47E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:3.62|P-value:2.47E-4 ID:SGTC_2612|Compound:dictamnine|FD-Score:8.24|P-value:8.63E-16 ID:SGTC_2714|Compound:st079313|FD-Score:-3.36|P-value:5.24E-4 ID:SGTC_2787|Compound:5284540|FD-Score:-3.51|P-value:3.12E-4 ID:SGTC_2790|Compound:5423970|FD-Score:-3.51|P-value:3.11E-4 ID:SGTC_2841|Compound:9004761|FD-Score:5.29|P-value:1.65E-7 ID:SGTC_2850|Compound:9015429|FD-Score:3.53|P-value:3.33E-4 ID:SGTC_2854|Compound:9024559|FD-Score:-3.33|P-value:5.84E-4 ID:SGTC_2977|Compound:9011197|FD-Score:6.04|P-value:2.73E-9 ID:SGTC_2983|Compound:9022671|FD-Score:4.70|P-value:2.85E-6 ID:SGTC_2987|Compound:9018338|FD-Score:4.93|P-value:9.85E-7 ID:SGTC_3054|Compound:9094397|FD-Score:-3.20|P-value:8.95E-4 ID:SGTC_3152|Compound:9099253|FD-Score:3.26|P-value:8.55E-4 ID:SGTC_3187|Compound:9109394|FD-Score:4.54|P-value:6.01E-6 ID:SGTC_3318|Compound:9139014|FD-Score:4.39|P-value:1.17E-5

Top fitness defect scores for YCL031C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_57 miconazole 9.97 162.5 nM 2.78E-22 ergosterol depletion effects on membrane 6 1.07E-23 0.48
2 SGTC_2612 dictamnine 8.24 15.5 µM 8.63E-16 RNA pol III & RNase P/MRP 11 8.97E-17 0.03
3 SGTC_256 clotrimazole 7.49 625.0 nM 2.34E-13 ergosterol depletion effects on membrane 4 3.53E-14 0.52
4 SGTC_682 0kpi-0099 7.44 17.0 µM 3.22E-13 11 4.97E-14 0.29
5 SGTC_2977 9011197 6.04 71.4 µM 2.73E-9 1 7.71E-10 0.65
6 SGTC_252 miconazole 5.90 1.3 nM 5.97E-9 ergosterol depletion effects on membrane 7 1.77E-9 0.35
7 SGTC_539 4859-0462 5.67 2.5 µM 2.15E-8 RNA pol III & RNase P/MRP 10 6.97E-9 0.47
8 SGTC_1018 4259-0013 5.67 28.1 µM 2.17E-8 1 7.02E-9 0.84
9 SGTC_168 3970-0862 5.44 530.5 µM 7.74E-8 22 2.73E-8 0.02
10 SGTC_2841 9004761 5.29 39.0 µM 1.65E-7 amide catabolism 4 6.14E-8 0.66
11 SGTC_2093 5331342 5.18 194.4 µM 2.94E-7 1 1.13E-7 0.70
12 SGTC_1722 st034304 5.03 3.6 µM 5.97E-7 mitochondrial processes 6 2.41E-7 0.01
13 SGTC_601 0986-0029 4.95 62.9 µM 8.90E-7 calcium & mitochondrial duress 11 3.70E-7 0.22
14 SGTC_453 enantio-paf c16 4.93 10.0 µM 9.65E-7 11 4.03E-7 0.08
15 SGTC_2987 9018338 4.93 71.4 µM 9.85E-7 RPP1 & pyrimidine depletion 2 4.12E-7 0.51
16 SGTC_2494 18-aminoabieta-8,11,13-triene sulfate 4.84 3.7 µM 1.54E-6 5 6.63E-7 0.04
17 SGTC_2983 9022671 4.70 13.0 µM 2.85E-6 60S ribosome export 6 1.28E-6 0.02
18 SGTC_1133 3473-0247 4.58 50.0 µM 4.97E-6 RSC complex & mRNA processing 39 2.32E-6 0.04
19 SGTC_3187 9109394 4.54 49.5 µM 6.01E-6 Golgi 3 2.84E-6 0.17
20 SGTC_3318 9139014 4.39 38.6 µM 1.17E-5 7 5.78E-6 0.01

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.212 2.46E-35 YGR090W UTP22 het Component of the small-subunit processome; required for nuclear export of tRNAs; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals
0.196 1.94E-30 YER008C SEC3 het Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress
0.186 2.01E-27 YBR102C EXO84 het Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate
0.180 7.26E-26 YGL233W SEC15 het Essential 113 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; interacts with and functions as a downstream effector of active, GTP-bound Sec4p, a Rab family GTPase
0.177 6.14E-25 YML126C ERG13 het 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis
0.155 1.31E-19 YPR055W SEC8 het Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress
0.143 6.95E-17 YLR424W SPP382 het Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation
0.142 1.21E-16 YIL068C SEC6 het Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p
0.136 2.04E-15 YJL195C_d YJL195C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6
0.136 2.68E-15 YMR277W FCP1 het Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p)
0.132 1.43E-14 YML101C-A_d YML101C-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.132 1.64E-14 YAL038W CDC19 het Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication
0.125 3.60E-13 YLR374C_d YLR374C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF STP3/YLR375W
0.122 1.25E-12 YOR333C_d YOR333C_d hom Dubious open reading frame, unlikely to encode a functional protein; overlaps 5' end of MRS2 gene required for respiratory growth
0.122 1.39E-12 YOR260W GCD1 het Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression