YCL032W / STE50

Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction

Zygosity: Homozygous strain
fixedexpanded
Profile for YCL032W / STE50

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:3.36|P-value:7.17E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:6.17|P-value:2.60E-9 ID:SGTC_73|Compound:0327-0325|FD-Score:3.71|P-value:2.20E-4 ID:SGTC_109|Compound:0132-0036|FD-Score:-4.98|P-value:1.35E-6 ID:SGTC_119|Compound:0121-0043|FD-Score:-5.26|P-value:3.55E-7 ID:SGTC_160|Compound:3453-0671|FD-Score:3.26|P-value:9.95E-4 ID:SGTC_162|Compound:k030-0015|FD-Score:-3.73|P-value:2.22E-4 ID:SGTC_163|Compound:1327-0036|FD-Score:8.19|P-value:4.70E-15 ID:SGTC_170|Compound:4197-0004|FD-Score:-3.29|P-value:9.65E-4 ID:SGTC_210|Compound:3551-0445|FD-Score:4.35|P-value:1.94E-5 ID:SGTC_223|Compound:busulfan|FD-Score:3.27|P-value:9.74E-4 ID:SGTC_226|Compound:1,3-diallylurea|FD-Score:4.22|P-value:3.29E-5 ID:SGTC_261|Compound:5-fluorouridine|FD-Score:6.75|P-value:8.71E-11 ID:SGTC_306|Compound:1672-3436|FD-Score:-3.58|P-value:3.73E-4 ID:SGTC_516|Compound:3992-2719|FD-Score:3.45|P-value:5.36E-4 ID:SGTC_674|Compound:0498-0004|FD-Score:-3.55|P-value:4.09E-4 ID:SGTC_934|Compound:2950-0684|FD-Score:3.59|P-value:3.34E-4 ID:SGTC_936|Compound:3005-4620|FD-Score:3.85|P-value:1.31E-4 ID:SGTC_1061|Compound:3448-9325|FD-Score:-3.68|P-value:2.58E-4 ID:SGTC_1109|Compound:tnp00067|FD-Score:4.17|P-value:3.98E-5 ID:SGTC_1253|Compound:0550-0034|FD-Score:-3.31|P-value:9.21E-4 ID:SGTC_1263|Compound:0774-1208|FD-Score:3.49|P-value:4.75E-4 ID:SGTC_1314|Compound:1222-0010|FD-Score:3.52|P-value:4.23E-4 ID:SGTC_1487|Compound:3970-0795|FD-Score:3.52|P-value:4.26E-4 ID:SGTC_1533|Compound:dihydroergotamine mesylate|FD-Score:6.15|P-value:2.98E-9 ID:SGTC_1551|Compound:6-bromo-3'-methylflavone|FD-Score:3.90|P-value:1.10E-4 ID:SGTC_2005|Compound:4024620|FD-Score:5.94|P-value:9.69E-9 ID:SGTC_2008|Compound:4012253|FD-Score:3.77|P-value:1.78E-4 ID:SGTC_2009|Compound:4012297|FD-Score:-4.08|P-value:6.08E-5 ID:SGTC_2161|Compound:5662504|FD-Score:5.19|P-value:4.53E-7 ID:SGTC_2167|Compound:5876490|FD-Score:3.53|P-value:4.16E-4 ID:SGTC_2336|Compound:9001819|FD-Score:5.42|P-value:1.44E-7 ID:SGTC_2549|Compound:cedrol|FD-Score:4.82|P-value:2.50E-6 ID:SGTC_2553|Compound:4-methylesculetin|FD-Score:3.28|P-value:9.34E-4 ID:SGTC_2594|Compound:a-mangostin|FD-Score:4.40|P-value:1.55E-5 ID:SGTC_2778|Compound:tioconazole|FD-Score:3.82|P-value:1.50E-4 ID:SGTC_2849|Compound:9019321|FD-Score:-3.30|P-value:9.31E-4 ID:SGTC_2870|Compound:9038528|FD-Score:3.28|P-value:9.53E-4 ID:SGTC_2906|Compound:7926292|FD-Score:-5.38|P-value:1.91E-7 ID:SGTC_2908|Compound:7930301|FD-Score:-4.18|P-value:4.14E-5 ID:SGTC_2909|Compound:7941780|FD-Score:-4.86|P-value:2.29E-6 ID:SGTC_2912|Compound:7964298|FD-Score:4.03|P-value:6.89E-5 ID:SGTC_2940|Compound:9060638|FD-Score:3.29|P-value:9.03E-4 ID:SGTC_2973|Compound:9094665|FD-Score:3.83|P-value:1.46E-4 ID:SGTC_3083|Compound:9117465|FD-Score:3.39|P-value:6.59E-4 ID:SGTC_3189|Compound:9110682|FD-Score:4.08|P-value:5.59E-5 ID:SGTC_3264|Compound:9137442|FD-Score:-3.30|P-value:9.37E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:3.36|P-value:7.17E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:6.17|P-value:2.60E-9 ID:SGTC_73|Compound:0327-0325|FD-Score:3.71|P-value:2.20E-4 ID:SGTC_109|Compound:0132-0036|FD-Score:-4.98|P-value:1.35E-6 ID:SGTC_119|Compound:0121-0043|FD-Score:-5.26|P-value:3.55E-7 ID:SGTC_160|Compound:3453-0671|FD-Score:3.26|P-value:9.95E-4 ID:SGTC_162|Compound:k030-0015|FD-Score:-3.73|P-value:2.22E-4 ID:SGTC_163|Compound:1327-0036|FD-Score:8.19|P-value:4.70E-15 ID:SGTC_170|Compound:4197-0004|FD-Score:-3.29|P-value:9.65E-4 ID:SGTC_210|Compound:3551-0445|FD-Score:4.35|P-value:1.94E-5 ID:SGTC_223|Compound:busulfan|FD-Score:3.27|P-value:9.74E-4 ID:SGTC_226|Compound:1,3-diallylurea|FD-Score:4.22|P-value:3.29E-5 ID:SGTC_261|Compound:5-fluorouridine|FD-Score:6.75|P-value:8.71E-11 ID:SGTC_306|Compound:1672-3436|FD-Score:-3.58|P-value:3.73E-4 ID:SGTC_516|Compound:3992-2719|FD-Score:3.45|P-value:5.36E-4 ID:SGTC_674|Compound:0498-0004|FD-Score:-3.55|P-value:4.09E-4 ID:SGTC_934|Compound:2950-0684|FD-Score:3.59|P-value:3.34E-4 ID:SGTC_936|Compound:3005-4620|FD-Score:3.85|P-value:1.31E-4 ID:SGTC_1061|Compound:3448-9325|FD-Score:-3.68|P-value:2.58E-4 ID:SGTC_1109|Compound:tnp00067|FD-Score:4.17|P-value:3.98E-5 ID:SGTC_1253|Compound:0550-0034|FD-Score:-3.31|P-value:9.21E-4 ID:SGTC_1263|Compound:0774-1208|FD-Score:3.49|P-value:4.75E-4 ID:SGTC_1314|Compound:1222-0010|FD-Score:3.52|P-value:4.23E-4 ID:SGTC_1487|Compound:3970-0795|FD-Score:3.52|P-value:4.26E-4 ID:SGTC_1533|Compound:dihydroergotamine mesylate|FD-Score:6.15|P-value:2.98E-9 ID:SGTC_1551|Compound:6-bromo-3'-methylflavone|FD-Score:3.90|P-value:1.10E-4 ID:SGTC_2005|Compound:4024620|FD-Score:5.94|P-value:9.69E-9 ID:SGTC_2008|Compound:4012253|FD-Score:3.77|P-value:1.78E-4 ID:SGTC_2009|Compound:4012297|FD-Score:-4.08|P-value:6.08E-5 ID:SGTC_2161|Compound:5662504|FD-Score:5.19|P-value:4.53E-7 ID:SGTC_2167|Compound:5876490|FD-Score:3.53|P-value:4.16E-4 ID:SGTC_2336|Compound:9001819|FD-Score:5.42|P-value:1.44E-7 ID:SGTC_2549|Compound:cedrol|FD-Score:4.82|P-value:2.50E-6 ID:SGTC_2553|Compound:4-methylesculetin|FD-Score:3.28|P-value:9.34E-4 ID:SGTC_2594|Compound:a-mangostin|FD-Score:4.40|P-value:1.55E-5 ID:SGTC_2778|Compound:tioconazole|FD-Score:3.82|P-value:1.50E-4 ID:SGTC_2849|Compound:9019321|FD-Score:-3.30|P-value:9.31E-4 ID:SGTC_2870|Compound:9038528|FD-Score:3.28|P-value:9.53E-4 ID:SGTC_2906|Compound:7926292|FD-Score:-5.38|P-value:1.91E-7 ID:SGTC_2908|Compound:7930301|FD-Score:-4.18|P-value:4.14E-5 ID:SGTC_2909|Compound:7941780|FD-Score:-4.86|P-value:2.29E-6 ID:SGTC_2912|Compound:7964298|FD-Score:4.03|P-value:6.89E-5 ID:SGTC_2940|Compound:9060638|FD-Score:3.29|P-value:9.03E-4 ID:SGTC_2973|Compound:9094665|FD-Score:3.83|P-value:1.46E-4 ID:SGTC_3083|Compound:9117465|FD-Score:3.39|P-value:6.59E-4 ID:SGTC_3189|Compound:9110682|FD-Score:4.08|P-value:5.59E-5 ID:SGTC_3264|Compound:9137442|FD-Score:-3.30|P-value:9.37E-4

Top fitness defect scores for YCL032W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_163 1327-0036 8.19 7.5 µM 4.70E-15 18 1.30E-16
2 SGTC_261 5-fluorouridine 6.75 15.4 µM 8.71E-11 exosome 1 7.58E-12
3 SGTC_64 000l-0018 6.17 272.1 µM 2.60E-9 ubiquinone biosynthesis & proteasome 77 3.35E-10
4 SGTC_1533 dihydroergotamine mesylate 6.15 29.4 µM 2.98E-9 DNA intercalators 2 3.91E-10
5 SGTC_2005 4024620 5.94 188.0 µM 9.69E-9 3 1.46E-9
6 SGTC_2336 9001819 5.42 198.7 µM 1.44E-7 heme biosynthesis & mitochondrial translocase 14 2.95E-8
7 SGTC_2161 5662504 5.19 8.5 µM 4.53E-7 tubulin folding & SWR complex 7 1.06E-7
8 SGTC_2549 cedrol 4.82 20.9 µM 2.50E-6 6 7.11E-7
9 SGTC_2594 a-mangostin 4.40 100.0 µM 1.55E-5 21 5.40E-6
10 SGTC_210 3551-0445 4.35 56.8 µM 1.94E-5 52 6.92E-6
11 SGTC_226 1,3-diallylurea 4.22 8.3 mM 3.29E-5 10 1.25E-5
12 SGTC_1109 tnp00067 4.17 928.3 nM 3.98E-5 12 1.54E-5
13 SGTC_3189 9110682 4.08 49.5 µM 5.59E-5 32 2.25E-5
14 SGTC_2912 7964298 4.03 10.9 µM 6.89E-5 cell wall 92 2.84E-5
15 SGTC_1551 6-bromo-3'-methylflavone 3.90 63.5 µM 1.10E-4 16 4.79E-5
16 SGTC_936 3005-4620 3.85 401.0 µM 1.31E-4 22 5.82E-5
17 SGTC_2973 9094665 3.83 18.0 µM 1.46E-4 NEO1 32 6.53E-5
18 SGTC_2778 tioconazole 3.82 65.1 nM 1.50E-4 ergosterol depletion effects on membrane 66 6.75E-5
19 SGTC_2008 4012253 3.77 172.0 µM 1.78E-4 30 8.15E-5
20 SGTC_73 0327-0325 3.71 16.2 µM 2.20E-4 DNA damage response 57 1.03E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.152 1.06E-18 YGR193C PDX1 hom Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) of the mitochondrial pyruvate dehydrogenase (PDH) complex, plays a structural role in the complex by binding and positioning E3 to the dihydrolipoamide acetyltransferase (E2) core
0.149 4.23E-18 YJR013W GPI14 het Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M
0.140 3.33E-16 YIR008C PRI1 het Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair
0.125 3.91E-13 YIR012W SQT1 het Protein involved in 60S ribosomal subunit assembly or modification; contains multiple WD repeats; interacts with Qsr1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress
0.121 2.10E-12 YPL209C IPL1 het Aurora kinase subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), involved in regulating kinetochore-microtubule attachments; helps maintain condensed chromosomes during anaphase and early telophase; required for SPB cohesion and prevention of multipolar spindle formation; localizes to nuclear foci that become diffuse upon DNA replication stress; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon SAC arrest
0.119 3.90E-12 YER089C PTC2 hom Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p to limit maximal osmostress induced kinase activity; dephosphorylates Ire1p to downregulate the unfolded protein response; dephosphorylates Cdc28p; inactivates the DNA damage checkpoint; PTC2 has a paralog, PTC3, that arose from the whole genome duplication
0.118 6.03E-12 YDR537C_d YDR537C_d hom Dubious open reading frame unlikely to encode a protein, almost completely overlaps verified ORF PAD1/YDR538W
0.117 9.00E-12 YFL037W TUB2 het Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria
0.116 1.87E-11 YLR101C_d YLR101C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF ERG27/YLR100W
0.116 1.91E-11 YML103C NUP188 hom Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC organization and nucleocytoplasmic transport; homologous to human NUP188
0.114 3.27E-11 YDL189W RBS1 hom Protein of unknown function, identified as a high copy suppressor of psk1 psk2 mutations that confer temperature-sensitivity for galactose utilization; proposed to bind single-stranded nucleic acids via its R3H domain
0.105 1.03E-9 YER052C HOM3 hom Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
0.105 1.27E-9 YLR331C_d JIP3_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
0.104 1.41E-9 YIL129C TAO3 het Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with protein kinase Cbk1p and also with Kic1p
0.104 1.75E-9 YKL048C ELM1 hom Serine/threonine protein kinase that regulates cellular morphogenesis, septin behavior, and cytokinesis; required for the regulation of other kinases, such as Kin4p; forms part of the bud neck ring