YCL040W / GLK1

Glucokinase; catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources; GLK1 has a paralog, EMI2, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YCL040W / GLK1

Click on Significant Values for Screen Details ID:SGTC_49|Compound:0717-0965|FD-Score:4.25|P-value:1.14E-5 ID:SGTC_66|Compound:0109-0187|FD-Score:7.91|P-value:1.54E-15 ID:SGTC_77|Compound:0828-0286|FD-Score:3.56|P-value:2.00E-4 ID:SGTC_137|Compound:0438-0332|FD-Score:3.16|P-value:8.33E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:3.82|P-value:7.09E-5 ID:SGTC_163|Compound:1327-0036|FD-Score:3.67|P-value:1.30E-4 ID:SGTC_315|Compound:k915-0449|FD-Score:-4.60|P-value:1.96E-6 ID:SGTC_330|Compound:1488-0319|FD-Score:3.46|P-value:2.88E-4 ID:SGTC_583|Compound:1488-1091|FD-Score:3.51|P-value:2.37E-4 ID:SGTC_656|Compound:4090-1979|FD-Score:-3.30|P-value:4.61E-4 ID:SGTC_694|Compound:1315-0438|FD-Score:3.38|P-value:3.86E-4 ID:SGTC_700|Compound:3453-2283|FD-Score:-3.36|P-value:3.69E-4 ID:SGTC_716|Compound:1683-5115|FD-Score:3.62|P-value:1.56E-4 ID:SGTC_767|Compound:0929-0023|FD-Score:3.56|P-value:2.02E-4 ID:SGTC_769|Compound:0302-0231|FD-Score:3.20|P-value:7.21E-4 ID:SGTC_784|Compound:4579-0127|FD-Score:4.25|P-value:1.16E-5 ID:SGTC_789|Compound:1309-1135|FD-Score:3.13|P-value:9.21E-4 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:4.54|P-value:3.09E-6 ID:SGTC_1049|Compound:3226-0134|FD-Score:3.53|P-value:2.25E-4 ID:SGTC_1093|Compound:fluphenazine|FD-Score:4.70|P-value:1.44E-6 ID:SGTC_1125|Compound:tnp00248|FD-Score:-3.73|P-value:9.05E-5 ID:SGTC_1189|Compound:1431-2119|FD-Score:3.43|P-value:3.24E-4 ID:SGTC_1209|Compound:2323-2347|FD-Score:4.11|P-value:2.16E-5 ID:SGTC_1343|Compound:1487-0095|FD-Score:3.21|P-value:6.96E-4 ID:SGTC_1486|Compound:3970-0793|FD-Score:3.21|P-value:7.16E-4 ID:SGTC_1625|Compound:st003711|FD-Score:3.68|P-value:1.23E-4 ID:SGTC_1771|Compound:st048882|FD-Score:-4.51|P-value:2.97E-6 ID:SGTC_1773|Compound:st048542|FD-Score:4.04|P-value:2.89E-5 ID:SGTC_1908|Compound:st060353|FD-Score:7.70|P-value:8.22E-15 ID:SGTC_1919|Compound:st067113|FD-Score:-5.86|P-value:2.15E-9 ID:SGTC_1942|Compound:st076330|FD-Score:4.22|P-value:1.31E-5 ID:SGTC_1949|Compound:st076024|FD-Score:3.74|P-value:9.99E-5 ID:SGTC_2017|Compound:5132927|FD-Score:4.39|P-value:6.28E-6 ID:SGTC_2111|Compound:5486399|FD-Score:3.37|P-value:4.02E-4 ID:SGTC_2241|Compound:6772625|FD-Score:3.52|P-value:2.30E-4 ID:SGTC_2274|Compound:7947094|FD-Score:-3.33|P-value:4.16E-4 ID:SGTC_2328|Compound:6201408|FD-Score:-3.55|P-value:1.80E-4 ID:SGTC_2421|Compound:5690543|FD-Score:3.89|P-value:5.37E-5 ID:SGTC_2424|Compound:5537928|FD-Score:4.78|P-value:9.57E-7 ID:SGTC_2530|Compound:3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene|FD-Score:3.69|P-value:1.22E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:4.88|P-value:5.74E-7 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:3.64|P-value:1.44E-4 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:-3.27|P-value:5.08E-4 ID:SGTC_2785|Compound:7756463|FD-Score:4.88|P-value:5.88E-7 ID:SGTC_2853|Compound:9015042|FD-Score:3.24|P-value:6.26E-4 ID:SGTC_2856|Compound:9022925|FD-Score:-3.47|P-value:2.48E-4 ID:SGTC_2857|Compound:9024691|FD-Score:-3.56|P-value:1.76E-4 ID:SGTC_2936|Compound:9038235|FD-Score:3.22|P-value:6.74E-4 ID:SGTC_2995|Compound:9067767|FD-Score:3.55|P-value:2.04E-4 ID:SGTC_3004|Compound:9076122|FD-Score:3.62|P-value:1.60E-4 ID:SGTC_3044|Compound:9093193|FD-Score:4.77|P-value:9.84E-7 ID:SGTC_3056|Compound:9094900|FD-Score:3.28|P-value:5.59E-4 ID:SGTC_3202|Compound:9113761|FD-Score:3.20|P-value:7.43E-4 ID:SGTC_3205|Compound:9113483|FD-Score:3.16|P-value:8.36E-4 ID:SGTC_3209|Compound:9113616|FD-Score:3.74|P-value:1.00E-4 ID:SGTC_3232|Compound:9132874|FD-Score:3.89|P-value:5.47E-5 ID:SGTC_3244|Compound:9134489|FD-Score:3.18|P-value:7.82E-4 ID:SGTC_3285|Compound:9112818|FD-Score:3.13|P-value:9.35E-4 ID:SGTC_3313|Compound:9133968|FD-Score:-3.09|P-value:9.55E-4 ID:SGTC_49|Compound:0717-0965|FD-Score:4.25|P-value:1.14E-5 ID:SGTC_66|Compound:0109-0187|FD-Score:7.91|P-value:1.54E-15 ID:SGTC_77|Compound:0828-0286|FD-Score:3.56|P-value:2.00E-4 ID:SGTC_137|Compound:0438-0332|FD-Score:3.16|P-value:8.33E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:3.82|P-value:7.09E-5 ID:SGTC_163|Compound:1327-0036|FD-Score:3.67|P-value:1.30E-4 ID:SGTC_315|Compound:k915-0449|FD-Score:-4.60|P-value:1.96E-6 ID:SGTC_330|Compound:1488-0319|FD-Score:3.46|P-value:2.88E-4 ID:SGTC_583|Compound:1488-1091|FD-Score:3.51|P-value:2.37E-4 ID:SGTC_656|Compound:4090-1979|FD-Score:-3.30|P-value:4.61E-4 ID:SGTC_694|Compound:1315-0438|FD-Score:3.38|P-value:3.86E-4 ID:SGTC_700|Compound:3453-2283|FD-Score:-3.36|P-value:3.69E-4 ID:SGTC_716|Compound:1683-5115|FD-Score:3.62|P-value:1.56E-4 ID:SGTC_767|Compound:0929-0023|FD-Score:3.56|P-value:2.02E-4 ID:SGTC_769|Compound:0302-0231|FD-Score:3.20|P-value:7.21E-4 ID:SGTC_784|Compound:4579-0127|FD-Score:4.25|P-value:1.16E-5 ID:SGTC_789|Compound:1309-1135|FD-Score:3.13|P-value:9.21E-4 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:4.54|P-value:3.09E-6 ID:SGTC_1049|Compound:3226-0134|FD-Score:3.53|P-value:2.25E-4 ID:SGTC_1093|Compound:fluphenazine|FD-Score:4.70|P-value:1.44E-6 ID:SGTC_1125|Compound:tnp00248|FD-Score:-3.73|P-value:9.05E-5 ID:SGTC_1189|Compound:1431-2119|FD-Score:3.43|P-value:3.24E-4 ID:SGTC_1209|Compound:2323-2347|FD-Score:4.11|P-value:2.16E-5 ID:SGTC_1343|Compound:1487-0095|FD-Score:3.21|P-value:6.96E-4 ID:SGTC_1486|Compound:3970-0793|FD-Score:3.21|P-value:7.16E-4 ID:SGTC_1625|Compound:st003711|FD-Score:3.68|P-value:1.23E-4 ID:SGTC_1771|Compound:st048882|FD-Score:-4.51|P-value:2.97E-6 ID:SGTC_1773|Compound:st048542|FD-Score:4.04|P-value:2.89E-5 ID:SGTC_1908|Compound:st060353|FD-Score:7.70|P-value:8.22E-15 ID:SGTC_1919|Compound:st067113|FD-Score:-5.86|P-value:2.15E-9 ID:SGTC_1942|Compound:st076330|FD-Score:4.22|P-value:1.31E-5 ID:SGTC_1949|Compound:st076024|FD-Score:3.74|P-value:9.99E-5 ID:SGTC_2017|Compound:5132927|FD-Score:4.39|P-value:6.28E-6 ID:SGTC_2111|Compound:5486399|FD-Score:3.37|P-value:4.02E-4 ID:SGTC_2241|Compound:6772625|FD-Score:3.52|P-value:2.30E-4 ID:SGTC_2274|Compound:7947094|FD-Score:-3.33|P-value:4.16E-4 ID:SGTC_2328|Compound:6201408|FD-Score:-3.55|P-value:1.80E-4 ID:SGTC_2421|Compound:5690543|FD-Score:3.89|P-value:5.37E-5 ID:SGTC_2424|Compound:5537928|FD-Score:4.78|P-value:9.57E-7 ID:SGTC_2530|Compound:3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene|FD-Score:3.69|P-value:1.22E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:4.88|P-value:5.74E-7 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:3.64|P-value:1.44E-4 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:-3.27|P-value:5.08E-4 ID:SGTC_2785|Compound:7756463|FD-Score:4.88|P-value:5.88E-7 ID:SGTC_2853|Compound:9015042|FD-Score:3.24|P-value:6.26E-4 ID:SGTC_2856|Compound:9022925|FD-Score:-3.47|P-value:2.48E-4 ID:SGTC_2857|Compound:9024691|FD-Score:-3.56|P-value:1.76E-4 ID:SGTC_2936|Compound:9038235|FD-Score:3.22|P-value:6.74E-4 ID:SGTC_2995|Compound:9067767|FD-Score:3.55|P-value:2.04E-4 ID:SGTC_3004|Compound:9076122|FD-Score:3.62|P-value:1.60E-4 ID:SGTC_3044|Compound:9093193|FD-Score:4.77|P-value:9.84E-7 ID:SGTC_3056|Compound:9094900|FD-Score:3.28|P-value:5.59E-4 ID:SGTC_3202|Compound:9113761|FD-Score:3.20|P-value:7.43E-4 ID:SGTC_3205|Compound:9113483|FD-Score:3.16|P-value:8.36E-4 ID:SGTC_3209|Compound:9113616|FD-Score:3.74|P-value:1.00E-4 ID:SGTC_3232|Compound:9132874|FD-Score:3.89|P-value:5.47E-5 ID:SGTC_3244|Compound:9134489|FD-Score:3.18|P-value:7.82E-4 ID:SGTC_3285|Compound:9112818|FD-Score:3.13|P-value:9.35E-4 ID:SGTC_3313|Compound:9133968|FD-Score:-3.09|P-value:9.55E-4

Top fitness defect scores for YCL040W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_66 0109-0187 7.91 42.4 µM 1.54E-15 3 1.32E-15
2 SGTC_1908 st060353 7.70 56.5 µM 8.22E-15 RPP1 & pyrimidine depletion 3 7.06E-15
3 SGTC_2567 aureobasidin a 4.88 60.0 nM 5.74E-7 60 5.23E-7
4 SGTC_2785 7756463 4.88 72.7 µM 5.88E-7 10 5.36E-7
5 SGTC_2424 5537928 4.78 59.2 µM 9.57E-7 19 8.74E-7
6 SGTC_3044 9093193 4.77 49.5 µM 9.84E-7 13 8.99E-7
7 SGTC_1093 fluphenazine 4.70 8.0 µM 1.44E-6 7 1.32E-6
8 SGTC_915 methyl methanesulfonate (MMS) 4.54 110.0 mM 3.09E-6 DNA damage response 25 2.83E-6
9 SGTC_2017 5132927 4.39 60.6 µM 6.28E-6 20 5.78E-6
10 SGTC_49 0717-0965 4.25 32.1 µM 1.14E-5 60S ribosome export 16 1.06E-5
11 SGTC_784 4579-0127 4.25 173.0 µM 1.16E-5 15 1.07E-5
12 SGTC_1942 st076330 4.22 65.2 µM 1.31E-5 23 1.21E-5
13 SGTC_1209 2323-2347 4.11 50.3 µM 2.16E-5 13 2.00E-5
14 SGTC_1773 st048542 4.04 73.6 µM 2.89E-5 40 2.68E-5
15 SGTC_2421 5690543 3.89 90.7 µM 5.37E-5 21 4.98E-5
16 SGTC_3232 9132874 3.89 49.5 µM 5.47E-5 RPP1 & pyrimidine depletion 15 5.08E-5
17 SGTC_159 3970-0959 3.82 50.0 µM 7.09E-5 80 6.60E-5
18 SGTC_1949 st076024 3.74 69.1 µM 9.99E-5 34 9.31E-5
19 SGTC_3209 9113616 3.74 49.5 µM 1.00E-4 31 9.32E-5
20 SGTC_2530 3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene 3.69 92.6 µM 1.22E-4 34 1.14E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.125 4.17E-13 YKL004W AUR1 het Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance
0.105 1.11E-9 YGR228W_d YGR228W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C
0.103 2.06E-9 YJR013W GPI14 het Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M
0.103 2.58E-9 YOR147W MDM32 hom Mitochondrial inner membrane protein with similarity to Mdm31p, required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MDM10, MDM12, and MDM34
0.102 2.94E-9 YGL074C_d YGL074C_d het Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor
0.097 1.94E-8 YCL033C MXR2 hom Methionine-R-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR1; involved in the regulation of lifespan
0.096 2.67E-8 YDR367W KEI1 het Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene
0.096 3.06E-8 YIL061C SNP1 het Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress
0.095 3.36E-8 YLR456W_p YLR456W_p hom Putative pyridoxal 5'-phosphate synthase; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2; YLR456W has a paralog, YPR172W, that arose from the whole genome duplication
0.095 3.94E-8 YLR044C PDC1 hom Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde; subject to glucose-, ethanol-, and autoregulation; involved in amino acid catabolism
0.094 5.17E-8 YLR429W CRN1 hom Coronin, cortical actin cytoskeletal component that associates with the Arp2p/Arp3p complex to regulate its activity; plays a role in regulation of actin patch assembly
0.092 1.04E-7 YCL009C ILV6 hom Regulatory subunit of acetolactate synthase, which catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria
0.089 2.72E-7 YDR488C PAC11 hom Dynein intermediate chain, acts in the cytoplasmic dynein pathway, forms cortical cytoplasmic microtubule capture site with Num1p; null mutant is defective in nuclear migration, essential in the absence of CIN8
0.088 2.94E-7 YNL216W RAP1 het Essential DNA-binding transcription regulator that binds at many loci; involved in either transcription activation or repression, chromatin silencing, and telomere length maintenance; conserved protein with an N-terminal BRCT domain, a central region with homology to the Myb DNA binding domain, and a C-terminal Rap1-specific protein-interaction domain (RCT domain)
0.087 4.33E-7 YIR008C PRI1 het Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair