HIPHOP chemogenomics database

YCL040W / GLK1

Glucokinase; catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources; GLK1 has a paralog, EMI2, that arose from the whole genome duplication

Zygosity: Homozygous strain

fixedexpanded
Profile for YCL040W / GLK1

Click on Significant Values for Screen Details

Top fitness defect scores for YCL040W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_66	0109-0187	7.91	42.4 µM	1.54E-15		3	1.32E-15
2	SGTC_1908	st060353	7.70	56.5 µM	8.22E-15	RPP1 & pyrimidine depletion	3	7.06E-15
3	SGTC_2567	aureobasidin a	4.88	60.0 nM	5.74E-7		60	5.23E-7
4	SGTC_2785	7756463	4.88	72.7 µM	5.88E-7		10	5.36E-7
5	SGTC_2424	5537928	4.78	59.2 µM	9.57E-7		19	8.74E-7
6	SGTC_3044	9093193	4.77	49.5 µM	9.84E-7		13	8.99E-7
7	SGTC_1093	fluphenazine	4.70	8.0 µM	1.44E-6		7	1.32E-6
8	SGTC_915	methyl methanesulfonate (MMS)	4.54	110.0 mM	3.09E-6	DNA damage response	25	2.83E-6
9	SGTC_2017	5132927	4.39	60.6 µM	6.28E-6		20	5.78E-6
10	SGTC_49	0717-0965	4.25	32.1 µM	1.14E-5	60S ribosome export	16	1.06E-5
11	SGTC_784	4579-0127	4.25	173.0 µM	1.16E-5		15	1.07E-5
12	SGTC_1942	st076330	4.22	65.2 µM	1.31E-5		23	1.21E-5
13	SGTC_1209	2323-2347	4.11	50.3 µM	2.16E-5		13	2.00E-5
14	SGTC_1773	st048542	4.04	73.6 µM	2.89E-5		40	2.68E-5
15	SGTC_2421	5690543	3.89	90.7 µM	5.37E-5		21	4.98E-5
16	SGTC_3232	9132874	3.89	49.5 µM	5.47E-5	RPP1 & pyrimidine depletion	15	5.08E-5
17	SGTC_159	3970-0959	3.82	50.0 µM	7.09E-5		80	6.60E-5
18	SGTC_1949	st076024	3.74	69.1 µM	9.99E-5		34	9.31E-5
19	SGTC_3209	9113616	3.74	49.5 µM	1.00E-4		31	9.32E-5
20	SGTC_2530	3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene	3.69	92.6 µM	1.22E-4		34	1.14E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.125	4.17E-13	YKL004W	AUR1	het	Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance
0.105	1.11E-9	YGR228W_d	YGR228W_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C
0.103	2.06E-9	YJR013W	GPI14	het	Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M
0.103	2.58E-9	YOR147W	MDM32	hom	Mitochondrial inner membrane protein with similarity to Mdm31p, required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MDM10, MDM12, and MDM34
0.102	2.94E-9	YGL074C_d	YGL074C_d	het	Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor
0.097	1.94E-8	YCL033C	MXR2	hom	Methionine-R-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR1; involved in the regulation of lifespan
0.096	2.67E-8	YDR367W	KEI1	het	Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene
0.096	3.06E-8	YIL061C	SNP1	het	Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress
0.095	3.36E-8	YLR456W_p	YLR456W_p	hom	Putative pyridoxal 5'-phosphate synthase; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2; YLR456W has a paralog, YPR172W, that arose from the whole genome duplication
0.095	3.94E-8	YLR044C	PDC1	hom	Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde; subject to glucose-, ethanol-, and autoregulation; involved in amino acid catabolism
0.094	5.17E-8	YLR429W	CRN1	hom	Coronin, cortical actin cytoskeletal component that associates with the Arp2p/Arp3p complex to regulate its activity; plays a role in regulation of actin patch assembly
0.092	1.04E-7	YCL009C	ILV6	hom	Regulatory subunit of acetolactate synthase, which catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria
0.089	2.72E-7	YDR488C	PAC11	hom	Dynein intermediate chain, acts in the cytoplasmic dynein pathway, forms cortical cytoplasmic microtubule capture site with Num1p; null mutant is defective in nuclear migration, essential in the absence of CIN8
0.088	2.94E-7	YNL216W	RAP1	het	Essential DNA-binding transcription regulator that binds at many loci; involved in either transcription activation or repression, chromatin silencing, and telomere length maintenance; conserved protein with an N-terminal BRCT domain, a central region with homology to the Myb DNA binding domain, and a C-terminal Rap1-specific protein-interaction domain (RCT domain)
0.087	4.33E-7	YIR008C	PRI1	het	Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair